miRTarBase - #MIRT589502


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miRNA Infomation
miRNA namemmu-miR-1186a

Gene Information
Gene Symbol Myo1e   
Synonyms 2310020N23Rik, 9130023P14Rik, AA407778, myosin-1e, myr 3
Description myosin IE
Transcript NM_181072   
Expression
Putative miRNA Targets on Myo1e
3'UTR of Myo1e
(miRNA target sites are highlighted)		 
>Myo1e|NM_181072|3'UTR
   1 GGCGCTCGGAAACTGACACGTGGGCAGAGGAGCTCCAGGCGTGGACCGGGGAGGGGACTTTTGGGGACTCCTCTTCTGAT
  81 CGACAGTGAGCAACTGCTACACCAAGGCCTGAAGCTATTCTGACACCTTCCCCCATGGAAGACACAGAAAAGCTTGGGTT
 161 AGGGACAAGGACAAGTTATGGGTCACTCCACAAATGATCCTAAAAGGTAGTCTAGTTCCCAGGAGCGCAAAAAACACCAC
 241 CCACTAAGATTTATTTATTGTATTTAAATCTGGTGTAAGTAGCGGGGACTGCTTGGTGTCTATCCCACAGCCTCCTGCCC
 321 ACTCACCAGGTCCAGGGGACAGCAGGCGGTGACTCGATGACAAGTGCCTTTAGTTGAAGAGCACATCTCACTCATTCCTC
 401 TCTCAGTACCTGATACATTCCTCTGTGCTAACCCCCCCTTGGGGAGGACCCACCCTCTGGAGGCTGGACTTGGGGCGAAC
 481 AGGCACTCACCTGTCACTGCCAAGGGCGGGCAGGCCATCCTTCCGAGCCCATGGGAGCCGGGACCACTAAGACTGCTGGT
 561 GGGAAGAAGTTGGGTGCTGGGCTGATGGTCTTGCTTTCTCTTGGTCTTCGCTTGTAATGTGGCTGGCCCATGTTGGTTTT
 641 ATGTTTAATGCTGTGCTTATAATAAGAAAGAGCCCCCCCAAGCTGTACATTTATAAAAAGTGATCATATACTGTATATAG
 721 AAAAATCTAGAAGCACATATGAATGCAGCAGGTAGTATTCCACTGTACCCATTCATGAAGGTAGGTTTTATTACAGGACT
 801 CGCACCAGGTACTTACAGACGCGCCCTCTCCTCTTTGCCTAGAGAAACAGTCACTGCATTCCCGCACAGTCCCTCAGACC
 881 CCCTTACCCTCTCCCTGTAGGAAATTCTCCTGTGACCCCTCTGCCGTCCTCCCTTACTTCCTAAATAAATGTAACGGAGT
 961 CAGTGCAATGCCTTGTTTCTTCATGACATTTATTGTGGGTTATAATTTTCTCCTAAAAACAAAACCAGTGGTATGGTCAT
1041 ACCCACCATTGTTTCCCCACTTTCCATGACCGTCACAAACATCTGGGATGAGCACCTTGTGAGCAGGAAAAGTTATGCTT
1121 TAAGAAATTTCTGGCCAGGCGTGGTGGCATACACCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGTGGATTTCTGAGT
1201 TCGAGGCCAGCCTGGTCTACAAAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
114 mmu-miR-1186a Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT579774 Zfp568 zinc finger protein 568 1 1
MIRT580629 Sycp2 synaptonemal complex protein 2 1 1
MIRT582212 Nfatc3 nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 1 1
MIRT582800 Kcnmb2 potassium large conductance calcium-activated channel, subfamily M, beta member 2 1 1
MIRT585391 Wfs1 wolframin ER transmembrane glycoprotein 1 2
MIRT589502 Myo1e myosin IE 1 2
MIRT589770 Itgb3 integrin beta 3 1 1
MIRT593622 Tstd2 thiosulfate sulfurtransferase (rhodanese)-like domain containing 2 1 1
MIRT593854 Havcr2 hepatitis A virus cellular receptor 2 1 1
MIRT594849 Npm3 nucleoplasmin 3 1 1
MIRT596507 Zfp933 zinc finger protein 933 1 1
MIRT596526 Zfp882 zinc finger protein 882 1 1
MIRT596548 Zfp866 zinc finger protein 866 1 1
MIRT596772 Usp45 ubiquitin specific petidase 45 1 1
MIRT596785 Ubxn2a UBX domain protein 2A 1 1
MIRT596892 Trp53rk transformation related protein 53 regulating kinase B 1 1
MIRT596930 Tprkb Tp53rk binding protein 1 1
MIRT596959 Tmem88b transmembrane protein 88B 1 1
MIRT597152 Stxbp4 syntaxin binding protein 4 1 1
MIRT597426 Rp2h retinitis pigmentosa 2 homolog 1 1
MIRT597534 Rapgef4 Rap guanine nucleotide exchange factor (GEF) 4 1 1
MIRT597584 Ptpn7 protein tyrosine phosphatase, non-receptor type 7 1 1
MIRT597695 Ppp2r3d protein phosphatase 2 (formerly 2A), regulatory subunit B'', delta 1 1
MIRT598007 Npy1r neuropeptide Y receptor Y1 1 1
MIRT598031 Nol10 nucleolar protein 10 1 1
MIRT598115 Ncl nucleolin 1 1
MIRT598155 Mtif2 mitochondrial translational initiation factor 2 1 1
MIRT598268 Mettl2 methyltransferase like 2 1 1
MIRT598274 Med16 mediator complex subunit 16 1 1
MIRT598308 Mc1r melanocortin 1 receptor 1 1
MIRT598344 Mau2 MAU2 sister chromatid cohesion factor 1 1
MIRT598391 Magt1 magnesium transporter 1 1 1
MIRT598427 Lrrc14b leucine rich repeat containing 14B 1 1
MIRT598438 Lpl lipoprotein lipase 1 1
MIRT598663 Hpgds hematopoietic prostaglandin D synthase 1 1
MIRT598704 Haus2 HAUS augmin-like complex, subunit 2 1 1
MIRT598875 Glyctk glycerate kinase 1 1
MIRT598888 Glrx2 glutaredoxin 2 (thioltransferase) 1 1
MIRT598958 Gemin8 gem nuclear organelle associated protein 8 1 1
MIRT599163 Diap2 diaphanous related formin 2 1 1
MIRT599256 Tmem245 transmembrane protein 245 1 1
MIRT599387 Clmn calmin 1 1
MIRT599416 Chek2 checkpoint kinase 2 1 1
MIRT599521 Cbwd1 COBW domain containing 1 1 1
MIRT599544 Car5b carbonic anhydrase 5b, mitochondrial 1 1
MIRT599748 Anapc16 anaphase promoting complex subunit 16 1 1
MIRT599851 Adarb1 adenosine deaminase, RNA-specific, B1 1 1
MIRT599902 Acat1 acetyl-Coenzyme A acetyltransferase 1 1 1
MIRT599921 A830018L16Rik RIKEN cDNA A830018L16 gene 1 1
MIRT599997 4930579G24Rik RIKEN cDNA 4930579G24 gene 1 1
MIRT600230 Trim56 tripartite motif-containing 56 1 1
MIRT600272 Tns4 tensin 4 1 1
MIRT600359 Srrm4 serine/arginine repetitive matrix 4 1 1
MIRT600536 Pdzd2 PDZ domain containing 2 1 1
MIRT600568 Ociad2 OCIA domain containing 2 1 1
MIRT600688 Lrrc8e leucine rich repeat containing 8 family, member E 1 1
MIRT600869 Gad2 glutamic acid decarboxylase 2 1 1
MIRT600880 Gabpb2 GA repeat binding protein, beta 2 1 1
MIRT600957 Elf1 E74-like factor 1 1 1
MIRT601037 Cntn2 contactin 2 1 1
MIRT601117 Bri3bp Bri3 binding protein 1 1
MIRT601253 Abl2 v-abl Abelson murine leukemia viral oncogene 2 (arg, Abelson-related gene) 1 1
MIRT601372 Ybey ybeY metallopeptidase 1 1
MIRT601482 Tmod1 tropomodulin 1 1 1
MIRT601550 Tbc1d24 TBC1 domain family, member 24 1 1
MIRT601675 Sco1 SCO1 cytochrome c oxidase assembly protein 1 1
MIRT601939 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1 1
MIRT601945 Nwd1 NACHT and WD repeat domain containing 1 1 1
MIRT601976 Nav1 neuron navigator 1 1 1
MIRT602013 Mpp7 membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) 1 1
MIRT602121 Kremen2 kringle containing transmembrane protein 2 1 1
MIRT602189 Hyal1 hyaluronoglucosaminidase 1 1 1
MIRT602215 Hecw1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 1 1
MIRT602246 Grm1 glutamate receptor, metabotropic 1 1 1
MIRT602369 Fbxl3 F-box and leucine-rich repeat protein 3 1 1
MIRT602428 Ercc4 excision repair cross-complementing rodent repair deficiency, complementation group 4 1 1
MIRT602494 Dkc1 dyskeratosis congenita 1, dyskerin 1 1
MIRT602567 Csf2rb colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) 1 1
MIRT602597 Chrnb4 cholinergic receptor, nicotinic, beta polypeptide 4 1 1
MIRT602687 Camk4 calcium/calmodulin-dependent protein kinase IV 1 1
MIRT602764 Asxl2 additional sex combs like 2 (Drosophila) 1 1
MIRT602851 Abcd2 ATP-binding cassette, sub-family D (ALD), member 2 1 1
MIRT602908 2210016F16Rik RIKEN cDNA 2210016F16 gene 1 1
MIRT603036 Chic1 cysteine-rich hydrophobic domain 1 1 1
MIRT603112 Xcr1 chemokine (C motif) receptor 1 1 1
MIRT603146 Ubxn8 UBX domain protein 8 1 1
MIRT603156 Ubxn4 UBX domain protein 4 1 1
MIRT603435 Rpl7l1 ribosomal protein L7-like 1 1 1
MIRT603455 Rhbdl2 rhomboid, veinlet-like 2 (Drosophila) 1 1
MIRT603518 Pxmp4 peroxisomal membrane protein 4 1 1
MIRT603818 Kif5b kinesin family member 5B 1 1
MIRT603872 Il11 interleukin 11 1 1
MIRT603888 Il10rb interleukin 10 receptor, beta 1 1
MIRT603916 Hltf helicase-like transcription factor 1 1
MIRT603974 Gnal guanine nucleotide binding protein, alpha stimulating, olfactory type 1 1
MIRT604054 Erich1 glutamate rich 1 1 1
MIRT604186 Cxcl11 chemokine (C-X-C motif) ligand 11 1 1
MIRT604293 Ccdc36 coiled-coil domain containing 36 1 1
MIRT604399 Aida axin interactor, dorsalization associated 1 1
MIRT604524 Ccdc167 coiled-coil domain containing 167 1 1
MIRT604531 Zmym5 zinc finger, MYM-type 5 1 1
MIRT604577 Trip4 thyroid hormone receptor interactor 4 1 1
MIRT604627 Syt11 synaptotagmin XI 1 1
MIRT604718 Samd8 sterile alpha motif domain containing 8 1 1
MIRT604761 Rab27a RAB27A, member RAS oncogene family 1 1
MIRT604851 Mprip myosin phosphatase Rho interacting protein 1 1
MIRT604966 Gga2 golgi associated, gamma adaptin ear containing, ARF binding protein 2 1 1
MIRT605164 C030039L03Rik zinc finger protein 607B 1 1
MIRT605197 B4galt6 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 1 1
MIRT605486 Slc6a2 solute carrier family 6 (neurotransmitter transporter, noradrenalin), member 2 1 1
MIRT605618 Mpv17 MpV17 mitochondrial inner membrane protein 1 1
MIRT606168 Syt13 synaptotagmin XIII 1 1
MIRT606224 Rft1 RFT1 homolog 1 1
MIRT606518 BC026590 family with sequence similarity 206, member A 1 1

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