| pre-miRNA Information | |
|---|---|
| pre-miRNA | mmu-mir-1906-1 |
| Genomic Coordinates | chr12: 109544541 - 109544620 |
| Description | Mus musculus miR-1906-1 stem-loop |
| Comment | None |
| RNA Secondary Structure |  |
| pre-miRNA | mmu-mir-1906-2 |
| Genomic Coordinates | chrX: 88759474 - 88759553 |
| Synonyms | mmu-mir-1906, Mir1906 |
| Description | Mus musculus miR-1906-2 stem-loop |
| Comment | None |
| RNA Secondary Structure | |
| Mature miRNA Information | |
|---|---|
| Mature miRNA | mmu-miR-1906 |
| Sequence | 48| UGCAGCAGCCUGAGGCAGGGCU |69 |
| Evidence | Experimental |
| Experiments | Microarray |
| Putative Targets | |
| Gene Information | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene Symbol | Mtf1 | ||||||||||||||||||||
| Synonyms | MTF-1, Thyls | ||||||||||||||||||||
| Description | metal response element binding transcription factor 1 | ||||||||||||||||||||
| Transcript | NM_008636 | ||||||||||||||||||||
| Expression | |||||||||||||||||||||
| Putative miRNA Targets on Mtf1 | |||||||||||||||||||||
| 3'UTR of Mtf1 (miRNA target sites are highlighted) |
>Mtf1|NM_008636|3'UTR 1 ACTTCTCCCTCATCCCTGCCCCCCCCCCCCCCCTCCGCTGGACCCTCTTCCTGTGAGCACAGCAGACAGGCTGAGGCTCC 81 TCAGACCCTCCGAAACGTTAAGTGCCATGGATGTGTCAGAGTTCCTGTCCCTCCAGAGCCTGGACACCCCGTCCAATCCG 161 GTTCACAGTGAAGCACTACTGCAGGGGTAGAGGAAAGAGCCTGGCCGGCAGCTTCTCCAGGGGAAGGGCCTGTGCGCACA 241 CCCCTCCAGGAACAGGGTGAGCAGGAAGTACCGGCTGTGATGCTGCCGTCATGGGGTCAGAAATTGGAAGGATGAAGAAA 321 TCTGCCATCTGAAAGCTCACCTTTCAGACGTATTTTCTTTACTCGTATCCCAGGAACATCCATTTTAAGGAACTGATCTT 401 TGGAGGAAAAAACAAAAAACAAAAACAAAAAAAGAAAAAAAAAAGCTAAGTTATAAGTGAACTGTCTGGCTGCACTGTGT 481 GTCACTTTTGCTTATTGTTATGTGAACTTGGAAACTAAGGTTACGTGTATGCATAAAAGTTCTAAATGAAAGGGTGTGGT 561 TTCCATCACTTTGGTACTGCCCATCATTTGCACTGGGGTCACTGTGGATTGGGCAGGAGAGGCCACTGTGCCTGCCGGGT 641 GTTGCTTCTCTTCTGTGTCTGTTTAATCCGAGGCAGTACCTGGAGGGCCAGACCACCGCTCTATGAAAGCGGGGAGTGGC 721 AAGGGCAGGCGTGAGTTAGACTGGGTGAGTTGCTTTGTTGTTGGCACTTGGTTTCTGTGGAGCTTGGGGTAGATGCAGAG 801 GGGGCTGCCCTGTGTCCTGCTTAGTCCTAGCGGGCAGCTGCAGGCCTCCTGGCCAGAGGGAAGATGTGGTTCTGCAGGGC 881 CCCGAGGCAGTTGTTGACAGCTCTGTTGATAATGTGCTAGACCCTAGAGCTATCTAGCACAGCCACAGTCTTGCCTTCTT 961 GGCTCTTTCCATCTCTCAGTGCTTGTTAGTGCTTGAGGCTTGAAGTTCATCTCTCCTACGGAAGCGCCCTGTTTTATCCT 1041 AAAGTGAATGAGAGGCTTCTGGCCAGTGGCTGCCAGGGTCTTCCTGCCTTCGATGATGTTTGCTTCCTCAGAGCAGAAGG 1121 GCTGCTTTTCCAGAGAAAGTGCAGAGCTAAAGGAGTTAGATGGTGAGGCAGCAGGTGGTCGGCAGGCATTCTGGTACCCA 1201 GTTGTAGCCAGGCTTTGGTTATCCTCTCCCAGCTGCATGTCTCATGTCTCTTAGATTTGCATACAGCCTCATACAGTGCA 1281 AAGGAAGATGTGATCTCTAAACAAAAAGGAATAAAAACCTAAAATGATGATATTCTACTCAGGGTAAAACAAACAAACAA 1361 ACCCCTTCCACCAAAAAGCCTGAAGCTTGCAGTCAGTTTACCTCATTTGGAATGTTTTGTTGAGTTGTGTTACAGGAATT 1441 TTTTTTTATTAGTGTATAAAATTATACCCATCTCCTTGCCTTTGGCTCCTGGTTATTGCCTCTTCAAACATAAGTACAAT 1521 CTATAGGAGAATGGGGGTGTGGCTGTCCTTCGGCTGTTTACTGTAGCCATCAGTGCTTGGCAAGGTCAGAAGGTAGCTGG 1601 GTCTGGGACTGGAGGAGGCGTCTGTCTCCAGCAAAGAGCAGGGTCTGGTGCCCCAACAGGACAACCAGAGAGCTGTGTAG 1681 GTTGCCCTCCTGCAGTCTGGACCTTTCCTCAGAGCCAGCTAGGATGGTGACCCTTGTCTTTAGGCAAGGTATTTAGGGAC 1761 AATTGGCCAAGAGGGTCATGGCAGCACTATTGAGAAATGGCACAGGTACAAGCAGCTGATCTCCCATTCCAGGATTGGAC 1841 CAGGAGTATGAATTTCTGATGTTCTACACCCCCACACCCCACACACACCCAAAAAAGGTTGATGGATTTGGGCAACCATA 1921 GACCTCAGCAGGAATCTGCACGGTGTGAGGAGCCCCCTGTTGGCGCCGTGGTTAGTCCTTCTGGGAACCAGCTGCTTGGG 2001 TGTAGATTGTCCTCAGCTGTCTCTAGGACTTGCACTCACAGGAAGCTTTGCACTCGGGCCCTCACTCGTTTCTCTTCTCT 2081 GAGGAGAGCAGTGAATGGGACCCCCAAAGTGAGCTTGTAACAAGGGGTAGTAATGGCCCTCCCACTCACTCTGCCTATGA 2161 CACCCTGGGAGCTGACATAGGAAACAGGAGCCTAGGGAGGATGTATGGAAGGTTTCAATGGTTTAAATATGGCTTCTTGG 2241 TTTGCTAGGGCTGTGCTCTTAATGGTCCATTCCTAGGTTATTGGCTTTTCACCCTCCACAGTTGGCATGGTGAAGAAACA 2321 TTTGAGCAGTGTTGAGGCTGAAGTGTTGCTAGGTGAAGGAGCTAAGTGATTTTAGAACACAAGAGACTCTCAGAACCTAA 2401 GAGGTTTTGGAAAGTGAGGACTCTGCTTGCAGTTGGTGGTTGTGGAGAGCCAATGTGTGAAACAGGGTAGACAGACGCTT 2481 CGGCCATGCTTGTACCAGTGGTCCTTCCCCCATCTTTCCACAAGCCCAGCCTCTAATGCTAAGGACCTTGGAGAAGAGGG 2561 AACCTCCTGGCTAAACTAGAACCACGAAGACCCTTCATGGAGTGGTTTCGCCAGGGAATGACCTTAGGGTAAGGCCAAGA 2641 AGAGTGAATTCTGGCAAGTCGATTAAGCCTTTCCTTCCACGACAGCCATCACCACTGTCACCCTGTAGACGAGACTTGGG 2721 TTACCACTAGCATCTCCAGTTTAGCCTCTTCCAAGAGACTAGTTGGCAGCAGGGCTGATGGACCTAGGAGGTGGTCAGGT 2801 GATCTTGGTCCCACCCAATTGCCTTATAAATGTGGTCCTTCCCAGGCAGGAGGGTAAGGCTGAGAGAAGAGAGCTCAGTG 2881 CCCTTGGGCAGCACTAGTTTGGCCACAGGAGTACTGTCCTCTGTTGACTTTGGTTCATAAGATGATGGTACAATGCCAAG 2961 GAGAGGCTTGGGGTGTGGAGAGCCCTCTAGGTAGAAGGCAATGGGACTCCCCTTCCCTTTCAGAGTCAGTGGATGGGAAA 3041 AGGTTGTTTTCCGGATCAAGGCAGTGGGCTGATGGGGTAATGGAGGTGCCTGAGTTTTGCCTGAGGCTTTGTATTATGCT 3121 GAATGTGTCCAGAGGGACAAATTTGCAGAACCTCATATTGATATTTTAAATAATAAAATAAAAAGCACTTTAGGTTACTT 3201 TTATCTTTAACCCAATTGCTGCAATTTCTGTTGTGTATGTATATATACATATATATACTTTCCCCAAAGTTTTATTTTTT 3281 GCTCAGAATAAAAGTTAAGTTGAGGTGTAAAAAGAGCACTTACTTGGGTGCAATATATGCGTAGCTTGACAGTCGCTATC 3361 CCACGTGGCCCTGGCCTGGCCTTCTGCTCCATCAGCCCTGTGCTGAAGCTGGCCACAGGGAACCACTATCAGCATCTCAG 3441 CAGCTGCTCAATCTATGCAAGCCTTCCTGTGTGTCCGGGGCGCCCCCCTCAGGCCCTCCAAGGAACTGCTGCAGCTGTTT 3521 TCTTCTGACTGTTGAGGCCCCTTTTCAACTGCTTCTCCGCCGTCCCCTGCTCTTTCCCCTCTTCCCCAGAACAAAATGAT 3601 TCCTGAAAGGAAGGGTCGGTTGTTCCTAGGTAGAAACCTGGCACCTTTAGACTTTCATATTTGTAAACACATCCATTGAA 3681 GGGAAGCGTCTCCAGTGTCACTGAGACTGTTGGTGTGTGGAAGTGCACTACTGTCGCGAATTTACAGCTTTTTCACAGCC 3761 CCAATCAGAAATATGTCGGAAGGCTCTCTGCACCACCTCTGTTGGCTCAAAAGCAGAATGACTTCCACGCTTCATCAAAT 3841 CCATTTAGGAAGCCGTTAATGACCTCCCTCACCGGCTCCCACTTACTCTTAAATTCCCAACTGTAGAGGCATTTTAAATA 3921 TACTCCAGCCAGTGCAAGATTTTCACAGATGAAGTTTGCAAGTGGTTGTGCTGTGACACAGATGGGAAATTGTCCATCTC 4001 TCCTCTGAGGCAGAAGGGGATGAGCCATAAAACTGGATCATACATATTTCTGATTGGTCTGTTTCTTGCATATTCTTGGA 4081 TCTTGTTTCTTATGCCCCTGCTCTTGGGGAGGTTGGTCATTAGCCCAGGCATCTCTCACTGTTCTGGCGGCTTGAGGGCT 4161 TCTCAGTCTGCGTTGAAAATGCAAGTTTAATTGGATCTGTCAAATTATATTATAGTACAACCTTTTTCTAGAACTGGCGT 4241 GTAAAAACCATACACTGCTCCTTGGTGGTGGCTCACTGGGACAAAAATAGGTGATAATGTCATGTCACTTTCTCAATTCA 4321 ATGCAATTTCTACTTTTTTTTTTTTTAAACAGGTAATGTTTTCATAAAACTTTTTTCCACCAAAATCTGGGGTGTTTATT 4401 GTAGTATCCCCGTGTCCCTCGAGGTGCCAAGTCAGGGGCTTGCCCGCCCTTGCCGCGGGTGTTCTCAGGCTTCCCAAGGG 4481 CTGTGTGAGTCAACAGCCTGCATCTTTGGTTGTGCTTTGGAGAAAATGTCACTGAGGTTGGAGTCCCAGGTAGCTAGTCC 4561 CTTTTCCTGGGAATTCCCTGTTGCAGTTTGGGGAAGGTGGCTGGAAAACGGGCTTTTCTGGTTGCCTGGCTCAGATCTAG 4641 CCAGAAAAGGCCACAAACTCTTTTCTAACACAAACTAATCATTGGCCAGTGGTCTTGGTGACAAGTTTTTAAGTCCCAAA 4721 TAGTTTTATTTGAATTATGTAAAAGTACCAAGTTTATTTTAAATGTAAAACATGGGAACAACGGACTTCCACTGAGCGAT 4801 ATGAAAACGTTACAGGTTCAGTACTTCCAAAGGAAGAAACCTCCAACCCCTAAAAAGAATAAATACGAATTTGTATTTTT 4881 GAAGAATGTGAAATAATAGTGTTTGCTTAATTGCTCATTTTGTATAAACTTAATATTGTACTTTAAAATATCTGCTATGA 4961 AGTGAAAATTTAACTTTTTGGAATTGAAAAAGCAATATTAAATACTAATGAAATCTTAATTAAATGCTTATTTAAATCTG 5041 GTAGTACATGTGGCATTACTTCCCATCCCTGTCCCTGGTTGACACTCTCTTCCCACTCCCAGCCATCAAGTCTTGGAGGG 5121 ACAGAAAAGAAAGGTCAGTCACCAGGGTCTGCAGATTTCCTTTTAATCAAGACTCTGCTCAAGTGTTTTGTGGGGCTGAG 5201 AGCCCCCAAAGCATGAAATGAACATGTAATACCACCTGGAACCCCCAAAGCAGGCCAGACCACTCTGGCCAGCACTGCTG 5281 GCTTCCTGAATCCGAGTACTCAAGACTGGATGTTTGTTGGCTCCATTTCAAAGCACAGTACTGCCTTCAGCCAGGACGAC 5361 GTGGGAGTGAACCCAGCTGCTAGTAGAGTTGCCATCCCAGGCTGAGGGCCAAGTACCAGCAACTGCCCGTGAAGACTGGC 5441 CCCTTTTAGTGAAGGGA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
||||||||||||||||||||
| miRNA-target interactions (Predicted by miRanda) |
|
||||||||||||||||||||
| Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
|---|---|---|---|---|---|---|---|
| miRNA:Target | ---- | ||||||
| Validation Method |
|
||||||
| Conditions | C2C12 | ||||||
| Location of target site | 3'UTR | ||||||
| Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
| Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast
... - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell. |
||||||
| miRNA-target interactions (Provided by authors) |
|
||||||
| Article |
- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al. - Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
|
||||||
| CLIP-seq Support 1 for dataset GSM1385342 | |
|---|---|
| Method / RBP | HITS-CLIP / AGO2 |
| Cell line / Condition | C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast |
| Location of target site | NM_008636 | 3UTR | AAUUUCUGUUGUGUAUGUAUAUAUACAUAUAU |
| Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
| Article / Accession Series | PMID: 25083871 / GSE57596 |
| CLIP-seq Viewer | Link |
27 mmu-miR-1906 Target Genes:
Functional analysis:
ID |
Target | Description | Validation methods |
 |
 |
|||||||
| Strong evidence | Less strong evidence | |||||||||||
 |
 |
 |
 |
 |
 |
 |
 |
|||||
| MIRT578439 | Irgq | immunity-related GTPase family, Q |  |
|
2 | 10 | ||||||
| MIRT578971 | Dhdh | dihydrodiol dehydrogenase (dimeric) | |
|
2 | 4 | ||||||
| MIRT580446 | Tln2 | talin 2 | |
|
2 | 2 | ||||||
| MIRT582494 | Mbtps2 | membrane-bound transcription factor peptidase, site 2 | |
|
2 | 2 | ||||||
| MIRT587588 | Cox15 | cytochrome c oxidase assembly protein 15 | |
|
2 | 2 | ||||||
| MIRT589524 | Mtf1 | metal response element binding transcription factor 1 | |
|
2 | 2 | ||||||
| MIRT591281 | Klc1 | kinesin light chain 1 | |
|
2 | 4 | ||||||
| MIRT591935 | Ddx19b | DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b | |
|
2 | 2 | ||||||
| MIRT592084 | Sorcs2 | sortilin-related VPS10 domain containing receptor 2 | |
|
2 | 2 | ||||||
| MIRT592206 | Mapkap1 | mitogen-activated protein kinase associated protein 1 | |
|
2 | 4 | ||||||
| MIRT592349 | Armcx6 | armadillo repeat containing, X-linked 6 | |
|
2 | 4 | ||||||
| MIRT592351 | Angel1 | angel homolog 1 | |
|
2 | 2 | ||||||
| MIRT592365 | 4930444A02Rik | protein-O-mannose kinase | |
|
2 | 2 | ||||||
| MIRT592427 | Spsb4 | splA/ryanodine receptor domain and SOCS box containing 4 | |
|
2 | 2 | ||||||
| MIRT592671 | Itgav | integrin alpha V | |
|
2 | 2 | ||||||
| MIRT592712 | Fbxo21 | F-box protein 21 | |
|
2 | 4 | ||||||
| MIRT593155 | Itsn1 | intersectin 1 (SH3 domain protein 1A) | |
|
2 | 2 | ||||||
| MIRT593662 | Spen | SPEN homolog, transcriptional regulator (Drosophila) | |
|
2 | 2 | ||||||
| MIRT593976 | Cdc14b | CDC14 cell division cycle 14B | |
|
2 | 2 | ||||||
| MIRT597931 | Pacsin2 | protein kinase C and casein kinase substrate in neurons 2 | |
|
2 | 2 | ||||||
| MIRT597949 | Opa1 | OPA1, mitochondrial dynamin like GTPase | |
|
2 | 2 | ||||||
| MIRT598550 | Isoc1 | isochorismatase domain containing 1 | |
|
2 | 2 | ||||||
| MIRT599017 | Fgd4 | FYVE, RhoGEF and PH domain containing 4 | |
|
2 | 2 | ||||||
| MIRT600307 | Tacc1 | transforming, acidic coiled-coil containing protein 1 | |
|
2 | 2 | ||||||
| MIRT603647 | Otop1 | otopetrin 1 | |
|
2 | 2 | ||||||
| MIRT605000 | Fam168b | family with sequence similarity 168, member B | |
|
2 | 2 | ||||||
| MIRT606275 | Oxsm | 3-oxoacyl-ACP synthase, mitochondrial | |
|
2 | 2 | ||||||
| miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|