| miRNA Infomation | |
|---|---|
| miRNA name | mmu-miR-1186b |
| Gene Information | |
|---|---|
| Gene Symbol | Magt1 |
| Synonyms | 2410001C15Rik, 2610529C04Rik, 2810482I07Rik, IAG2, IAP |
| Description | magnesium transporter 1 |
| Transcript | NM_001190409 |
| Other Transcripts | NM_025952 |
| Expression | |
| Putative miRNA Targets on Magt1 | |
| 3'UTR of Magt1 (miRNA target sites are highlighted) |
>Magt1|NM_001190409|3'UTR 1 AGAGGATTCCAGAGAAACATTGACACTGGTAATGGAAACTGAAAAATAAAAACTATTTGGTGTTTGAAGAATGCAACTCA 81 CATATTTTATATTACCTCTTTTTGTCAAGTGATCTAAGTAGTTAATCATTAAACCAAAGATGTGTAGTGCCTTAACAAGC 161 AGTTGTAAAGATCATGAAGTGTTTGGAATTGTGCTCCTCCTAACCTTCCCTTCCCAATGAACTTAGGAAACATTTGGGGT 241 TTGTAGTATTAAGTACTTTATAAACATTTAAAACTACTACTTCATTTTACTTAGAGAAAAACTCAAAACTACTATAGTTA 321 ACTTGATTTTATCTCATCTAAGGTTGGGGATGGAAGGTTCTGAGCAGGAATCTCCACCTCTGCCTATTGCAAACAACTGC 401 ATCTGGAAGCCATTGTTCCTTTTCCTTCTTTGTGCAGATGTGTGTGCTTTACAGACCTTTCCTCTTGGGCCCAGGTATCA 481 TATACACTGTGTCATCTGAAAATGGACCGTGTCGCCTTTGGAAAGATACATGTCTTCCTGTGTCTTTCCACCTGAAATCA 561 GCTACATATATGCCTCCTTTTACACATTCAGAAGTACCTCTAACTAAAGACTATGATTTGTGCATGAGTGTGCTATAACT 641 ACCTTGTACATGGAAAGATTTCTCATTCTGTTTCTAGTTTTATTTAAAGTAGCCCTTCTCTAACAATAATAGTTTCTTGC 721 TAAAAATCAGTATAACTTATAAATGGTCTCAAATGTTTGTGTAAGTTACGCATTGTTATTGATTTCATTCTTATGTAACA 801 GAAAAATAAGCTGAGTTGTGTTCATGGAAAGGACTCCCTTAGAAGAATGAGAAAACACAATCATGAAATACTGATTTGTT 881 GCCTAGGTACAGAAATGAAATGGTTTGCCAGGCTGTGGTAGATGAAGGCTGAGCTCTGTGAGTTTGTGGTCAGCCTGGGC 961 TACATAGTGAGACCCTGTATCCAGGGGAAAAGAAAAAAAAAGGAAGGAAGGAAGAAAAGTAGCATGTTTTCAAGCTGAGG 1041 CTGGGGCTCAGAGGTATAGTGCTTGCCTAGCAAGAGTTACACTCTGTGTTCAATCCCAATGCCTTAGAAAGATTTCTTGA 1121 AACCTGGTCTGTGAATTTTTCCTTGTTTTGATAAGGACTGTTCCTTGAAACCTGGTCTTTTGTTTTTTAAAACACATGTT 1201 CGTAAAACATGGGGCTATTTTTTTAAAATAAGTGTTTCTTGAAACCTATTATGGCTCTTGTTTTCATTTTTGTTTTTGTG 1281 TGCCGGTTTTCTAAAAGCAATGTTCTTGAAACCTGTCTTTGAGACAGAGCTCTCGCTGCTCTTACCTCTCTTAGGGAGAG 1361 CTGTCCCGCCTTCAGCTCACATTCATCCTCCTGTCTCAGCTTCTCAAGTGCTGGGGTTGCAGGCTTGTGCCACCATTGAC 1441 TGTAATCTGCTTTTGCTATCAGTTTAGCTTTTTATTTACCCCGTCAATTAGAGATACAAAGAATGGTAGCAGGCACCTGC 1521 CTCTGCTCTTAGCTACCCTGCTGGCTGAAGTAGAATCCAAGAAGACCAAGAACTTGACACATTCTGGCATCATATTAACA 1601 CCTCATCTCAAAAATATGTCAGAAAAGAAAACTATGAAGGGATTTTGTTTGAATTTTATCTTTTTTTCCTGGTAGTGAAA 1681 ATAACCATATATGCTAACCAGTTTTCTATGAGGAATATTTAAATCATGGTTATAATATGGCTGACTCTGGCTTTCACCAT 1761 AGTTATTAAGAATAATCCAGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCTAAGGTCCTGAGT 1841 TCAAATCCCAGCAACCACATGGTGGCTCATAGCTATCCATAATGAGATCTGATGCCCTCTTCTGGTGTGTCTGAAGACAG 1921 CTACAGTGTCTTACATATAACAATAAATAAATCTTTAAAAAAAAATAGGTTTGCCAGGGCTAAAGAGATGGCTCAGTGGT 2001 TAAGAGCACTGACTGCTCTTCCAGAGATCATGAGTTCAATTCCCGGCAACCACATGGTGGCTCACAACCCTCTGTAATGG 2081 AATCTGATGCCTTCTTCTGGTGCACCTGAAGATAGCTACAGTGTACTTACATACTGTACATTAAATAAATAAATAAATAA 2161 ATGATCCAAAATGCTACTGTTAAGAAAGTGTACTCTTAGGCCGGGCAGTGGTGGCGCACACCTCTAACCCCAGCACTTGG 2241 GAGGCAGAGGCAGGCGGATTTCTGAGTTCCAGGACAGCCAGGGCTATAGAGAGAAACCCTGTCTCAAAAAAGAAAGAAAG 2321 AAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAGAGAGAGAAGG 2401 CAGGAGGCTTGGAGTGGAGCTCAGTGCTAGAGCACTTAAAAGGCTGTGGGTTCAATCCCAAATACCACAAAAATCTAGGA 2481 ATGTAACTGAAAAATGGAAAGACATATTAGAAACTACATTTAAAATATAGACATGGATATGTGACTGAGTTGATACAGTG 2561 CTTGCCTAGCCATGGGTTCAAACCACAACATCACATAAGCCGGGTATGGGTGTAAGCACATGTGTGTTATCCCAGTGCAC 2641 TCGGGAGACAGATGGGAACCAGAAGTTCACCCTCATCCTTGAGCATAGCGTGAGTATAAGGCCAACCTGAGCTACATAAA 2721 GACTCTATCCCAAAGACAAAACAAAAAACAAGAAAAGATACTGTGACTATTCCCTGCTATTGATCATGCAATAGCCTTCC 2801 CATGTCTTATATAATGGAGTTTATAGAAAGAGATGGACATAATTGACCCAGGAAAAAAATTTGGGGGTGATTTGTTGTGG 2881 TTGCTACAAATTTGATGACTAGAAGGATCATTCCTTGTAAATTGAGTGATTCTTAATTTCTTATCTGCTATTCATGTACT 2961 TGTCTCTGCCAAATGAATTTACGCTGTGATTCTTAGGAAACTAGTGTGAAGACTTGTCAAGTTGAACTGGGCAACACTCC 3041 TACTGTGTTATCTCTAGCTGCTGGTGCCTCCCGTGGCCAGTGTTTTGTGTGTTTTCTCCTTGTTTAAGAGCAACATTCTT 3121 GTATGGAGAAAGGCTTGCTCTGGCTAAAATCTGTGAAAAGCAGATTTTCTCATTTAAAATAGTGTTACTTTTGTGGAAAT 3201 TTTAAATTTAGCTTTTATCTGAAGTGCCTTAAGTAGATTCTTAATTTACTTTTCTAGTACTGGTTTGAATATAATTCATT 3281 ATGTATTTTTAATATACTGTTCAAAAATGATACATACTATAGCAAAGATAACCAAATGTCTGGCTTACTGCTTTCTGTCA 3361 ATAAACTTTTACAATCTAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
| Experimental Support 1 for Functional miRNA-Target Interaction | |
|---|---|
| miRNA:Target | ---- |
| Validation Method |
|
| Conditions | C2C12 |
| Location of target site | 3'UTR |
| Tools used in this research | TargetScan , miRTarCLIP , Piranha |
| Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast
... - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell. |
| Article |
- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al. - Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
|
| CLIP-seq Support 1 for dataset GSM1385342 | |
|---|---|
| Method / RBP | HITS-CLIP / AGO2 |
| Cell line / Condition | C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast |
| Location of target site | NM_001190409 | 3UTR | UCCUAAGGUCCUGAGUUCAA |
| Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
| Article / Accession Series | PMID: 25083871 / GSE57596 |
| CLIP-seq Viewer | Link |
86 mmu-miR-1186b Target Genes:
Functional analysis:
ID |
Target | Description | Validation methods |
 |
 |
|||||||
| Strong evidence | Less strong evidence | |||||||||||
 |
 |
 |
 |
 |
 |
 |
 |
|||||
| MIRT576999 | Mark2 | MAP/microtubule affinity regulating kinase 2 |  |
|
2 | 2 | ||||||
| MIRT585120 | Zfp935 | zinc finger protein 935 | |
|
2 | 2 | ||||||
| MIRT586283 | Prc1 | protein regulator of cytokinesis 1 | |
|
2 | 4 | ||||||
| MIRT586381 | Pbld1 | phenazine biosynthesis-like protein domain containing 1 | |
|
2 | 2 | ||||||
| MIRT586708 | Loxl2 | lysyl oxidase-like 2 | |
|
2 | 4 | ||||||
| MIRT587092 | Gla | galactosidase, alpha | |
|
2 | 4 | ||||||
| MIRT587538 | Cxcr2 | chemokine (C-X-C motif) receptor 2 | |
|
2 | 2 | ||||||
| MIRT589503 | Myo1e | myosin IE | |
|
2 | 4 | ||||||
| MIRT589590 | Magt1 | magnesium transporter 1 | |
|
2 | 2 | ||||||
| MIRT590954 | Stxbp2 | syntaxin binding protein 2 | |
|
2 | 2 | ||||||
| MIRT591055 | Rab11fip1 | RAB11 family interacting protein 1 (class I) | |
|
2 | 2 | ||||||
| MIRT591076 | Polr2l | polymerase (RNA) II (DNA directed) polypeptide L | |
|
2 | 2 | ||||||
| MIRT591337 | Gm10778 | predicted gene 10778 | |
|
2 | 2 | ||||||
| MIRT592001 | Wdr34 | WD repeat domain 34 | |
|
2 | 4 | ||||||
| MIRT592296 | Foxr1 | forkhead box R1 | |
|
2 | 4 | ||||||
| MIRT593950 | Cpne1 | copine I | |
|
2 | 2 | ||||||
| MIRT593956 | Cpm | carboxypeptidase M | |
|
2 | 2 | ||||||
| MIRT595194 | Tmtc3 | transmembrane and tetratricopeptide repeat containing 3 | |
|
2 | 2 | ||||||
| MIRT595287 | Mef2c | myocyte enhancer factor 2C | |
|
2 | 2 | ||||||
| MIRT596506 | Zfp933 | zinc finger protein 933 | |
|
2 | 2 | ||||||
| MIRT596786 | Ubxn2a | UBX domain protein 2A | |
|
2 | 2 | ||||||
| MIRT597061 | Tcea1 | transcription elongation factor A (SII) 1 | |
|
2 | 2 | ||||||
| MIRT597101 | Taf1a | TATA-box binding protein associated factor, RNA polymerase I, A | |
|
2 | 2 | ||||||
| MIRT597153 | Stxbp4 | syntaxin binding protein 4 | |
|
2 | 2 | ||||||
| MIRT597459 | Rhag | Rhesus blood group-associated A glycoprotein | |
|
2 | 2 | ||||||
| MIRT597814 | Pla2g2d | phospholipase A2, group IID | |
|
2 | 2 | ||||||
| MIRT597836 | Pik3c2a | phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha | |
|
2 | 2 | ||||||
| MIRT598087 | Nek7 | NIMA (never in mitosis gene a)-related expressed kinase 7 | |
|
2 | 2 | ||||||
| MIRT598275 | Med16 | mediator complex subunit 16 | |
|
2 | 2 | ||||||
| MIRT598705 | Haus2 | HAUS augmin-like complex, subunit 2 | |
|
2 | 2 | ||||||
| MIRT598887 | Glrx2 | glutaredoxin 2 (thioltransferase) | |
|
2 | 2 | ||||||
| MIRT598959 | Gemin8 | gem nuclear organelle associated protein 8 | |
|
2 | 2 | ||||||
| MIRT599390 | Clmn | calmin | |
|
2 | 2 | ||||||
| MIRT599417 | Chek2 | checkpoint kinase 2 | |
|
2 | 2 | ||||||
| MIRT599571 | Btc | betacellulin, epidermal growth factor family member | |
|
2 | 2 | ||||||
| MIRT599852 | Adarb1 | adenosine deaminase, RNA-specific, B1 | |
|
2 | 2 | ||||||
| MIRT599903 | Acat1 | acetyl-Coenzyme A acetyltransferase 1 | |
|
2 | 2 | ||||||
| MIRT599922 | A830018L16Rik | RIKEN cDNA A830018L16 gene | |
|
2 | 2 | ||||||
| MIRT599998 | 4930579G24Rik | RIKEN cDNA 4930579G24 gene | |
|
2 | 2 | ||||||
| MIRT600123 | Zfyve27 | zinc finger, FYVE domain containing 27 | |
|
2 | 2 | ||||||
| MIRT600437 | Samd8 | sterile alpha motif domain containing 8 | |
|
2 | 2 | ||||||
| MIRT600468 | Rbm4 | RNA binding motif protein 4 | |
|
2 | 2 | ||||||
| MIRT600872 | Gabpb2 | GA repeat binding protein, beta 2 | |
|
2 | 2 | ||||||
| MIRT601116 | Bri3bp | Bri3 binding protein | |
|
2 | 2 | ||||||
| MIRT601328 | Zfp689 | zinc finger protein 689 | |
|
2 | 2 | ||||||
| MIRT601448 | Tprkb | Tp53rk binding protein | |
|
2 | 2 | ||||||
| MIRT601451 | Tns4 | tensin 4 | |
|
2 | 2 | ||||||
| MIRT601483 | Tmod1 | tropomodulin 1 | |
|
2 | 2 | ||||||
| MIRT601536 | Tet1 | tet methylcytosine dioxygenase 1 | |
|
2 | 2 | ||||||
| MIRT601857 | Pkd2 | polycystic kidney disease 2 | |
|
2 | 2 | ||||||
| MIRT601946 | Nwd1 | NACHT and WD repeat domain containing 1 | |
|
2 | 2 | ||||||
| MIRT602015 | Mpp7 | membrane protein, palmitoylated 7 (MAGUK p55 subfamily member 7) | |
|
2 | 2 | ||||||
| MIRT602020 | Moap1 | modulator of apoptosis 1 | |
|
2 | 2 | ||||||
| MIRT602190 | Hyal1 | hyaluronoglucosaminidase 1 | |
|
2 | 2 | ||||||
| MIRT602217 | Hecw1 | HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 | |
|
2 | 2 | ||||||
| MIRT602370 | Fbxl3 | F-box and leucine-rich repeat protein 3 | |
|
2 | 2 | ||||||
| MIRT602416 | F2r | coagulation factor II (thrombin) receptor | |
|
2 | 2 | ||||||
| MIRT602429 | Ercc4 | excision repair cross-complementing rodent repair deficiency, complementation group 4 | |
|
2 | 2 | ||||||
| MIRT602495 | Dkc1 | dyskeratosis congenita 1, dyskerin | |
|
2 | 2 | ||||||
| MIRT602566 | Csf2rb | colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage) | |
|
2 | 2 | ||||||
| MIRT602668 | Car5b | carbonic anhydrase 5b, mitochondrial | |
|
2 | 2 | ||||||
| MIRT602688 | Camk4 | calcium/calmodulin-dependent protein kinase IV | |
|
2 | 2 | ||||||
| MIRT602852 | Abcd2 | ATP-binding cassette, sub-family D (ALD), member 2 | |
|
2 | 2 | ||||||
| MIRT602997 | Pdzd2 | PDZ domain containing 2 | |
|
2 | 2 | ||||||
| MIRT603145 | Ubxn8 | UBX domain protein 8 | |
|
2 | 2 | ||||||
| MIRT603436 | Rpl7l1 | ribosomal protein L7-like 1 | |
|
2 | 2 | ||||||
| MIRT603456 | Rhbdl2 | rhomboid, veinlet-like 2 (Drosophila) | |
|
2 | 2 | ||||||
| MIRT603635 | Pbxip1 | pre B cell leukemia transcription factor interacting protein 1 | |
|
2 | 2 | ||||||
| MIRT603725 | Mrpl51 | mitochondrial ribosomal protein L51 | |
|
2 | 2 | ||||||
| MIRT603750 | Mfsd2b | major facilitator superfamily domain containing 2B | |
|
2 | 2 | ||||||
| MIRT603819 | Kif5b | kinesin family member 5B | |
|
2 | 2 | ||||||
| MIRT603907 | Hpgds | hematopoietic prostaglandin D synthase | |
|
2 | 2 | ||||||
| MIRT603918 | Hltf | helicase-like transcription factor | |
|
2 | 2 | ||||||
| MIRT603975 | Gnal | guanine nucleotide binding protein, alpha stimulating, olfactory type | |
|
2 | 2 | ||||||
| MIRT604114 | Diap2 | diaphanous related formin 2 | |
|
2 | 2 | ||||||
| MIRT604187 | Cxcl11 | chemokine (C-X-C motif) ligand 11 | |
|
2 | 2 | ||||||
| MIRT604294 | Ccdc36 | coiled-coil domain containing 36 | |
|
2 | 2 | ||||||
| MIRT604400 | Aida | axin interactor, dorsalization associated | |
|
2 | 2 | ||||||
| MIRT604629 | Syt11 | synaptotagmin XI | |
|
2 | 2 | ||||||
| MIRT604707 | Scml4 | sex comb on midleg-like 4 (Drosophila) | |
|
2 | 2 | ||||||
| MIRT604967 | Gga2 | golgi associated, gamma adaptin ear containing, ARF binding protein 2 | |
|
2 | 2 | ||||||
| MIRT605165 | C030039L03Rik | zinc finger protein 607B | |
|
2 | 2 | ||||||
| MIRT605198 | B4galt6 | UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 | |
|
2 | 2 | ||||||
| MIRT605619 | Mpv17 | MpV17 mitochondrial inner membrane protein | |
|
2 | 2 | ||||||
| MIRT606468 | Tcaim | T cell activation inhibitor, mitochondrial | |
1 | 1 | |||||||
| MIRT606659 | Lsm11 | U7 snRNP-specific Sm-like protein LSM11 | |
|
2 | 2 | ||||||