Gene Information
Gene Symbol
Kitl
Synonyms
Clo, Con, Gb, Kitlg, Mgf, SCF, SF, SLF, Sl, blz, contrasted
Description
kit ligand
Transcript
NM_013598
Expression
Putative miRNA Targets on Kitl
3'UTR of Kitl
>Kitl|NM_013598|3'UTR
1 TTGTGGACGTATCAACATTGTTACCTTCGCACAGTGGCTGGTAACAGTTCATGTTTGCTTCATAAATGAAGCAGCCTTAA
81 ACAAATTCCCATTCTGTCTCAAGTGACAGACCTCATCCTTACCTGTTCTTGCTACCCGTGACCTTGTGTGGATGATTCAG
161 TTGTTGGAGCAGAGTGCTTCGCTGTGAACCCTGCACTGAATTATCATCTGTAAAGAAAAATCTGCACGGAGCAGGACTCT
241 GGAGGTTTTGCAAGTGATGATAGGGACAAGAACATGTGTCCAGTCTACTTGCACCGTTTGCATGGCTTGGGAAACGTCTG
321 AGTGCTGAAAACCCACCCAGCTTTGTTCTTCAGTCACAACCTGCAGCCTGTCGTTAATTATGGTCTCTGCAAGTAGATTT
401 CAGCCTGGATGGTGGGGGGAATTTTTTTTTTCACAAAGGGATGTAGAAAACAATTTAAAAAAACAAAACAAAACAATAAC
481 TCCGTAGCATCAGCCACTGTATGGAGTTGAAAGCCATGGGGAGTATTTTTTTTTCTTTTGAGCAGGCTCATCCGCTCTGT
561 AGTTAATGATTAAGGAGAGCAGCCTAAATGTAAGCTCCTCCTGACAGAAGTCATGGTGCACCGTATCCTACTAGAGAAAT
641 GGACATGGGTAGCTAGTTCTATCCATGCGGTGGAGCCCATGCTGAACTGCAGCATAATACCACGGCATATGGAAGACTCT
721 CCAGAAAAGTCCTTACAGGAGAAAATTGTCAACGTGGACCAGTGGAAGAACATGCCAAGGAAGACATTTGGCCCATCGTG
801 AAGTCAGTCTTTTCCCTTGACAGTCACTCTAGCGTGTGTAAATCGTGTTTGCAATAGTATAATGTGACATGCTTATGTTT
881 TGAAACTACTGCAGCGGTATGAAGACAGATATGGGAGGAATGCTGAATGTGCAAGGGTCTCCCCACCCCCACCCCCCCTT
961 TTATTTTAAATCCTGATGGGAGAGAATAAGAACGGATGATGGTATTTAGTACCTAAGATTAGAAATGGACACTTTATTCT
1041 AATACGGGAGTTGCAAACTAAGTCACTCATATTCTAAAAGAATGCAAGGACTCGGAAAACATTAGTTATTTGCAGTATAA
1121 CCGTTATCTCTTGAAACATAGCCACTTATTTAAATCGGGCACTCTGTATTCATTAAGGTTTATGAAACGAAATAAAAAAA
1201 GAGAGAGCTTTATGTAGCTGTGCATGTCAGTTTGCTATTTAAAACGTGTGACAGCATTTCTCATATTAGACAGAAATTGG
1281 CAGTGATTCCACACTGAGCATCGCGCTTTCAAACCAGAATTTAGAGGATGTGCTGTAGATGTCCTCCCCCCCCCCCCCCC
1361 GATAGAGAAAGCAATTGGCTAGACTCAAAGTATGACCTTTTCTTCTGCCTTTATTTCTGGTGAAGGTTCTCTTCACCTAC
1441 CGTAGACATACAAAACTTAAAGCTTTTCACACTAACTCAATTCTTATAGTAATTTTATTTGCCCTGTGCTAAAACCTT GTATAAATTGTATTGAAAGCCT TGTTTAAAACAAACAAAA Target sites
Provided by authors Predicted by miRanda DRVs SNPs DRVs & SNPs
miRNA-target interactions
ID
Duplex structure
Position
Score
MFE
1
miRNA 3' cauauugggcccgGUUUUCGAg 5'
||||||||
Target 5' tgtttaaaacaaaCAAAAGCTa 3'
3969 - 3990
145.00
-8.70
2
miRNA 3' cauAUUGGGCCCGGUUUUCGAg 5'
|: ||:| ||:|||| ||
Target 5' attTGCCCTGTGCTAAAACCTt 3'
1497 - 1518
135.00
-11.90
3
miRNA 3' caUAUUGGGCCCGGUUUUCGag 5'
|||| ::| :::|||||
Target 5' gtATAAATTGTATTGAAAGCct 3'
2523 - 2544
124.00
-8.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target
mmu-miR-208a-5p
-- Functional MTI --
Kitl
Validation Method
miRNA and mRNA specific interaction
Conditions
C2C12
Location of target site
3'UTR
Tools used in this research
TargetScan
,
miRTarCLIP
,
Piranha
Original Description (Extracted from the article)
...
HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast
...- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014,
Cell .
Article
- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.- Cell , 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [ PMID: 25083871 ]
CLIP-seq Support 1 for dataset GSM1385342
Method / RBP
HITS-CLIP / AGO2
Cell line / Condition
C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast
Location of target site
NM_013598 | 3UTR | UGUUUAAAACAAACAAAA
Tools used in this analysis
TargetScan, miRTarCLIP, and Piranha
Article / Accession Series
PMID: 25083871 / GSE57596
CLIP-seq Viewer
Link
miRNA-Drug Associations
miRNA
Small Melocule
FDA
CID
Detection Method
Condition
PMID
Year
Expression Pattern of miRNA
miR-208a
(S)-3,5-dihydroxyphenylglycine (DHPG)
NULL
443586 Quantitative real-time PCR
mouse brain
22309833 2012
up-regulated
miR-208a
Tert-butyl hydroperoxide (t-BHP)
NULL
6410 Microarray
mouse auditory cells
20510889 2010
down-regulated
miR-208a
Sulfonyl-hydrazone-1 (SHZ)
NULL
NULL
Quantitative real-time PCR
Murine broblast-derived Induced pluripotent stem cells
21445862 2011
up-regulated
miR-208a
1,2,6-Tri-O-galloyl-beta-D-glucopyranose
NULL
NULL
Microarray
HepG2 hepatocarcinoma cells.
22506400 2011
down-regulated
