miRTarBase - #MIRT590168


—
pre-miRNA Information
pre-miRNA mmu-mir-350   
Genomic Coordinates chr1: 176772325 - 176772423
Synonyms Mirn350, mmu-mir-350, Mir350
Description Mus musculus miR-350 stem-loop
Comment Kim et al. cloned 40 new miRNAs from rat E18 primary cortical neurons .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-350-3p
Sequence 61| UUCACAAAGCCCAUACACUUUC |82
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Elp4   
Synonyms A330107A17Rik, Paxneb
Description elongator acetyltransferase complex subunit 4
Transcript NM_023876   
Expression
Putative miRNA Targets on Elp4
3'UTR of Elp4
(miRNA target sites are highlighted)		 
>Elp4|NM_023876|3'UTR
   1 GGACCCTCCGTAGTCACCGAGTGCGAAGTTATCTGACACTCTGATTAGGACTGCTAGTAGTTCATGTAAACATTTGTCTT
  81 AATGATCTGACCCTCCAGACTTTGAGAAATACAAGGTCACAAAGTGGAGCCACCATCCTTAAGTTTGAGTTGTTTCATGA
 161 TTTATTTTTTTCTACTGACTTTTGTACAAACATAATATGGCAGAAATACTGCCATTTGACATTTAGCTGCTGTGGGTTTT
 241 TTTTTAAAATACTAATTACTTTTTAAATAAACACCCAAAAAATCCCCAAAATACAGTTTGCCTTAGAGTTGTCATTAGTT
 321 CAAGTGGACTATAGCAATGGGATGATGGTGACTTGTGTCTTATGGTAACAGGCATGGCACACCCCATAGAGTGTAGAACA
 401 TTGAATTCAAGTTGTGTGTGAATACATTTCAGATGACTTTGTTTTTGAAAAAATCACTACCAAGACTTTTTTTCAAAGAA
 481 TATTTTGTTTGCTAGTATTTGAAAGAGTTAGCATTTGTGCATCCCATTGTTGAAAATGGTGAAGACAAAAATCATATGTG
 561 GTGATTCAGTCTGCATTACCCAGCACATTAGACAATACATCCAGGACTTGATGGGAAGCAGGCAGGAAGACTCTGAAGTT
 641 CTGGGTAATTGACGTCAGCACTGTAACATTGTGTCAACAGACAAAATTCAAGCTGGCAGCTCCACTAATGCCGGGGTCCT
 721 AGAGATGTGTACTGAAGCCTGGTGAGCAAGTTATATAATCTAGGTCTAAGACAACGTAAGTAACTGAGACACACTCAGAC
 801 TTCCATTGTGCCTTTGTTAACCAGGAGCACTGGCTGAGCCTGTAAATGATATCTGACCATGCAAAATGTCTCGGGGACAC
 881 AGCATATGGAGCAGGTTTTCCTGAAAAACACTGGGCTTTGGGGTCATGGGTCCATAGTCTTGAAGGGTTTGACAAGCCAT
 961 AGATAAACAGTGGAAGCCAGAAAGCCACTTGTGTGTGTTTAAGAACTCCAGTTTTTTTAAAAGATATTTAACATCTTTTG
1041 GTAGAAATTGAATTTTCAAATAAATTAGAATATTTTAAAGCACCTTTAACTAGCATTTTAAGCAGTTTCCTTTTAATATA
1121 CCACAAATTATACTCTATTTTCTCTTGACAACATTGTCGAAAACAAAGACTAGGTTTTTAAAACTGTCTCACCAATATGA
1201 TGAATGTCAGTCACTTTAAAAAAAAAGCCCAGTGTAGCATAAACACGCACTGTATACAGTTCTCATTCAGAATTAGAATA
1281 ATTGAATCAATGACAGTGATTTGCCAGGATGTCAAATCTCTCCATCATATCCTGTCACTACCAGTTTATTTTTTGTGATA
1361 AAATCAAAAAGACAACTATTTTGTCACCAGGACTCATTAGCACTAAAAAATCAGGTTGATTTTTTTTTTTTTTTAGAATG
1441 TCTGGTTTATTTTTTTTAATCTCTTTAAATTCTCAAGACAAATTCTTCCCCATTTTCTTTTCAAAATAAAGTTTTAGAAT
1521 TGATCTGTCAGTTAAAATTAAAGGACTTTTGTATTAAAGAAGGGTACTTTTAATATTTAAAACAACCTCAAAAATTGATT
1601 TCCTCTATAAATTTTAAGTCTTGTTGCAGAGGTACCCAGGAAGTCCTGCTGTCCTCTAATGGGTTGTGAATAAACTGAAG
1681 TGAATGCTTGATTGTTTTAATAACTAACCTTTCTCTTACCCTGTTGTGATTCATTTTACACAAACACACAACAGCACAAT
1761 TTCTTGGCAGTTAGCTCTGAAAAGGAAGTGTGAGGCCATCAGGTGTGTAAATTATTCTAAAATATCTAATGAGTCATTGG
1841 TTTCTTTCTTGATTCCTATATCTAAAAAATTAACAATTGACTACTAGTAGTTATGTGCTTTGCAAATAGCTAAATTCCCT
1921 TGAAATTGAGTGAACATATTTGAACCCTCAGTTTAAATAGAGATTAAACTTTTCAAAGTTAATAAACCCACATTTTAAAT
2001 AAATACAGTACCTGGACTTTCCAAAAATACTACATTTACTGCTTTATTTTTCAGACTCAGTCAGCATATTTTAATACTGC
2081 TACTACTAAAAATAATAATGACAACAAAAAGACACTGCTATTGGAAATAGATGTATGGCATATTTTATTTTTTTTTTTGT
2161 CCTCTGACAAAAACCCAGTCTAAAAATATGTTTTCCTTTATTTGGAAATTTTTGTTCTCTAATACTGCACAACTTCCTAC
2241 ATGAAAATATTGGCCTTCAAAATGCTAACACACACACACAAGCACATGTCCTTCTAATATATTATGAAATGTACACTCTA
2321 TTCATAGTTTTTAACTTGTCTTGAGTATAAATACTTAATAACACTGTCAGAGTAACTGAACTGTCTTTGTATCAGCATCG
2401 CAGTGCATGAAGCCTGAGGCCCTGTGAAGTGATTCATTCCTCTGCTAGATCTGCCTTTCCTTAACATGGGTAAAGATTTT
2481 GACCACTCTAGGTTAACAAGGTATATGGATCTGAGACCATCAGATTTTAATAGCCACGAGACAAGATCACTTTTCTCAGC
2561 TTTTTTACTTATTCGTTAGCACTTCCCCAGGAAGAAGCTTGTAAAGTAAAGGCGATTCTTCTATTCTCTGTCTTTTTATT
2641 TTAATGACTGGAACATATACATACACTTACAGCAAGGGCATGTGGGGCAAACCATCGCTTAAGCCCATTATTAATGTCAC
2721 CTCCAAGATCTCATGTTATTATGTCCAAAGATAGTCACCATCAATAGGGTCCGAATTAAGCAAAAGATTTACACTTTGAT
2801 TTTGAAAAATGAGCAGTTTAGTCATCTGTGACAAAAAAAAAGTTACTAAAAAACAGAATAATGTATATTTGGCATCAGTT
2881 AACCAAACATAGAGACAAAAAGATCTTCTGGATAACATTAAGGCCCAGAGCACAAAGAATAAAAATGCTCGGTGCCTTCT
2961 GTGATGGCAGACATTCACAGGACGAGGCTGAACGCTAACGCTCTAGACAGAGTTCTGAGAAGCCTGCCTAGGATGTTTCC
3041 TTTAAGTAGAAAGAGGAGATGATGTGAGCAGTGTGGAGAAGCCGGTTGTTCACCAGATAAAAGTCGTCTCGAGGTGTAGC
3121 TTTGGAGGAGAGGAAATCCTCACAGTTTCATAACAGCGCATGGCAAAATTATTTTCACCTAAAGCAGCGTTCTCAACCTA
3201 TAAGTCATGACCTCTTAAAATCGAAACAGCCTTTCACAGGGGTCGTTCCTGCATATCAGATGTTTGCATTGCAGTTCATA
3281 ATAGTAGCAAGATTGCAGTTATGAAGTAGCAATGCGGTAATTTTATGATTGGGGTCACCCCAACATGAGGAACTCTATTA
3361 AAGAGTCACAGCATTGGGAAAGCTGACAGACCACTGCCCTAGAGTGATTCACCTGTAATTCTGTTCACCCAATGTCTCTC
3441 AGTCAGTGATAAAATGTAATCTGTATGCACCAAGATGTCATAAAATTTGGTCTTTGTCCTGGAAAATGACCCTAAAATAT
3521 CCCTACAGATTTTCTATTGACAAATCTATAAATATAACTGTCAGTAAATAAACAAATGAAAATGACCACTGGGAATCACA
3601 ACTCCCAGATACTGGGATGACACAGCCTGGGATATAGACAGCTCATCTCTCTAGGGTGCATGTGGGAAGAAGGCAAAGCT
3681 CATCTTGCTGAACCAGAGACACCCGCATCTGAGCTTCCTTCCTTTGGGATATACCAGACTGTCCCACTGCACTCTGGCAG
3761 CCTGGACCCTCTGATGACTTGTATATGTCTCAGACTGCACACATCCATATCCTCAGCTTTCATGTCACTCCCAGGTCTTG
3841 CACCCAGAATGCCCTGTGCCTTTCTGTCTGTGTGCTCTATTCAGCAGGGCCATGGGCCAGCACAGGATGCCTGACACCAG
3921 CCCAAATCACAGCCAGTTGAAAACTGCTCTCTTTTTTTTTCAAGGAATAAGTAAACCAGCCAGCAGAGGCTACAACACAG
4001 CTCTTCTCTGACAGTTCCCTCAACACTTGTCCCTCAGGGAACGGGAGGCTGCAAAATATCAGGAGGTGGAAGTGTCTGCA
4081 AGGGTGATTTGTACCAACAGAGGATATGTGCCAGCAGGCAGTGTGGGCTGACATGGACAACAACTGTGCATGCACGCACA
4161 CACACACATGCATACGCACACACACACACACACACACACACACACACACTGCAATCCAGTTAGGATGCTATCTCCGGACA
4241 TGAAGCAACTCCACTAGAATAAAATGGATCTCAATGATCTGTTCAGAAGTACCACATAGATGTCGAACTGCAAGGTTATG
4321 TTGAAATCCTCCAATCAATTTATTTCTGATCATTTGCCTTTTGCGATTTACAAAACCAAAAATCCTCAGAGTTGGGAGTC
4401 AGTGAAGTGCCTTCCCCCTAGGATGAGTCTCCTTGTATTTTTAATCTAGGTGGCCAGGACCCCAGAGTTTCTCTCTCTCA
4481 AGCTCGCTGAAGAAAATGTGTCTATGTGACTTAAGTGTTGCACAGAACAGGCAGAAAATAGCAGGCAGGCCTTTTTCTAA
4561 CCAACAAAAGCTTCCTTTTTTTTCTCTCCATTGCTTGGGCTCAGGCACTCCTGCCCTGCATGCTGCCACTTTAAACATGA
4641 TCAGATCTGTGCTTCTTCGCAAATGACAACTGACCAACAATGGGCCCTGCTTCATAGATTTGGGAATGTTTGGCTTAAGC
4721 TGCCAATGACTGAAGGCCTTTAACTCCCACCGGCCAGTCACAGTCCGCTTTGTTGTTGTCTCTTAGTGTGTCTGTGCTCA
4801 TTATTCCTTGACATGCAACATCCCCTCCACCCTCCAACCACCCACCACAGCACCGAAAGCAAGACACACATGGGAGCAGC
4881 TGGAGGGGTTCAATGGGAGCCGATGCCTCTTATGCAAAGAGTGGGGAGACAGGACAGCAAGAAGGGTTATCTGAGATTGG
4961 CAACAGCTGTCAGAGGATCTTGTTTAAGAACTCAACCTTTGAATATGACTGTAGTGGAATCATTGTGGGGATTGGTTGCC
5041 CAGGTTAAAAATGCCAGCTCTAATTCAAAACAATTCCTAGTGAATCCCTTGTTGCATTTTTTTAATTACTTCTACACTTT
5121 GAGCTTTAAAATCAAATAAATAGATAAATTTGAAATATTACTATTTTCTGTACACATTGTGGGATGCAGACCTACATACA
5201 GCTAGCTGTGTTTTGTTTTAGGTTTTTTTACAATACATTACCAACTTCTGATATCTGTAAAAATTATTTCTTTGAGGACC
5281 ATCAGGTTGTAAAATGGAATATATGAAAGTAACCATTGATTTAGGACATTAACCTAATGTGGGAACAAAATTTGTAACAC
5361 CACACTATAAGGTTAAAAAGAAAATAGTGTAATAAAGAAAATACAAAGGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cuuUCACAUACCCGAAACACUu 5'
             ||| |||   :||||||| 
Target 5' accAGTTTAT--TTTTTGTGAt 3'
 1340 - 1359 155.00 -7.40
2 
miRNA  3' cuUUCACAUACCCGAAACACuu 5'
            ||| || | |  ||||||  
Target 5' gaAAGAGT-TAGCATTTGTGca 3'
 501 - 521 135.00 -9.10
3 
miRNA  3' cuuucacaUACCCGAAACACUu 5'
                  |||||  |||||| 
Target 5' gtcctctaATGGG--TTGTGAa 3'
 1651 - 1670 129.00 -12.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' cuuUCACAUACCCGAAACACUu 5'
             ||| |||   :||||||| 
Target 5' --cAGUUUAU--UUUUUGUGAu 3'
 		1 - 18
Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
27 mmu-miR-350-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT435011 Lars2 leucyl-tRNA synthetase, mitochondrial 1 2
MIRT577176 Tet1 tet methylcytosine dioxygenase 1 1 5
MIRT577951 Pole4 polymerase (DNA-directed), epsilon 4 (p12 subunit) 1 1
MIRT579173 Cd70 CD70 antigen 1 1
MIRT580234 Tsga10 testis specific 10 1 1
MIRT581630 Prkaa1 protein kinase, AMP-activated, alpha 1 catalytic subunit 1 1
MIRT584029 D230025D16Rik RIKEN cDNA D230025D16 gene 1 1
MIRT588424 Zbtb6 zinc finger and BTB domain containing 6 1 1
MIRT588528 Usp45 ubiquitin specific petidase 45 1 2
MIRT588917 Skor1 SKI family transcriptional corepressor 1 1 1
MIRT589679 Larp1 La ribonucleoprotein domain family, member 1 1 2
MIRT590168 Elp4 elongator acetyltransferase complex subunit 4 1 1
MIRT593065 Ehf ets homologous factor 1 1
MIRT593183 BC068281 WD repeat and coiled coil containing 1 4
MIRT595160 Cbfa2t3 core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) 1 1
MIRT596758 Utp23 UTP23 small subunit processome component 1 1
MIRT596783 Ubxn2a UBX domain protein 2A 1 1
MIRT597289 Slc1a7 solute carrier family 1 (glutamate transporter), member 7 1 1
MIRT597494 Rbm41 RNA binding motif protein 41 1 1
MIRT600204 Ube2j2 ubiquitin-conjugating enzyme E2J 2 1 1
MIRT603448 Ric3 RIC3 acetylcholine receptor chaperone 1 1
MIRT604253 Cercam cerebral endothelial cell adhesion molecule 1 1
MIRT604915 Iqcj IQ motif containing J 1 1
MIRT605131 Cnnm3 cyclin M3 1 1
MIRT605214 Arnt aryl hydrocarbon receptor nuclear translocator 1 1
MIRT605697 Ifnar1 interferon (alpha and beta) receptor 1 1 1
MIRT606256 Polr1e polymerase (RNA) I polypeptide E 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-350 Hesperidin NULL 10621 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-350 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) NULL 16078949 Microarray hepatocytes 23107762 2013 down-regulated
miR-350 Tamoxifen approved 2733526 Microarray rat liver 17343880 2007 down-regulated
miR-350 Ursodeoxycholic acid (UDCA) approved 31401 Microarray rat Liver 20689055 2010 down-regulated

Error report submission