miRTarBase - #MIRT590630


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pre-miRNA Information
pre-miRNA mmu-mir-452   
Genomic Coordinates chrX: 72262224 - 72262308
Synonyms Mirn452, mmu-mir-452, Mir452
Description Mus musculus miR-452 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-452-3p
Sequence 53| UCAGUCUCAUCUGCAAAGAGGU |74
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Arl5a   
Synonyms 2410015N24Rik, 2810410P22Rik, AA408731, AW610751, Arl5, T25534
Description ADP-ribosylation factor-like 5A
Transcript NM_182994   
Expression
Putative miRNA Targets on Arl5a
3'UTR of Arl5a
(miRNA target sites are highlighted)		 
>Arl5a|NM_182994|3'UTR
   1 TCTCTTCTGACCCCTCCTCACAGACTCTGTATAAAGAAAGTGCTGGACTTTTCCTGAAAGCTGCAAAAAAATGGTTTAGA
  81 TATATTTATAATAAACTGACTTAAGAGTTTTCTATAAGAAGAAAATTAAGACCACTTATTTGAAAACAAAGATGAAATGT
 161 CACCACCCGTTTGCGCTCTCATTCCTTCCAAGCCTGTGACTGGTATTTTCTCATGATGAATCCTCTCAGGACATGGCAGA
 241 GCTTGGCGAGTACAAAGGGAGAGGAAGACATTTGAACTTTAGAACTTTGTTTCAGCGTGACTCTCCCACCCTGCCCCAAG
 321 TTGAATGTTCCCTTCACATTGAATCAAATACAAATCAGCACAGATACTGTTTCCAGTTTTTAAAAATGTAATATTATGGA
 401 AAGTATTTTGCTTCAAGTTGAATATGTATTGTGTATATACCTCAAGTTCAGGTTAATGGCTTTGATTTGTGCTCTAAAGC
 481 AAAGCAAATAACACAGGTTAATGGTAACTTGTTGTTCTCCTGATCTAACTGAGTACCACCTTTGGTGTTAAGTGTCATTG
 561 TTTAGTTTACCCTCTGTTCTCTATGTTGTCCTAACAAGCCAACCCCAACCAACAATTGTTAAGCTGCTCCTTAAAAATGA
 641 AACAGGAGAAAGCCTGGCATTGTATACCTGTTTTTGTTGCTCAGTAATTCAGGTGGAAATATATAACGCAAGTGGGTAGA
 721 GCGTCTGCCAGACTCCTGGCCTGGTGTGCCTGGGTAGAATTACACGATAAGCAATGGTCAGACTTTCCTTCTGTGGATCC
 801 TTATGCCAATTCAGCTACTAATCGGTCATTCATAAATGGTGATTAAAATGATCAGGTAATTTTTAATTGAAAATCATGGT
 881 GTGAAATATATCTACCCAAAGAGGTAACATCCTATAAATAGTAGTAATTAATTTAGCTGCAACCTTTTTAGGTATTAAAT
 961 TACATGACAAACAGTTAAAATGAATTTAAGAATAAACATAAGGCATCGTTTTCAATGAACAGTAATAATTTCCCAATTTA
1041 CAGATTAGTGTCTACAAAGGTTTTCTTACATCTTCTTCCATTGCATAATATGCCTTGTGTAATAGTTAAGAAATCATTTT
1121 TTCTGAATGACTTAAAAAATTTGATTGAAAATTTGGCAAATTACATTATTTGTACTAGAATTTGACCTAAAACACTTTTT
1201 ATGTTTGTTAAAAATATTTGCTTTTTAAAATTTAGTTAAAAATTGAAGTATTTCTGTCATAGACAAAATTACTCTGTTTT
1281 AACATATTCCTGGTGGACTATATCAACCTTTTGTGCCCTGAAAACTTGAAGGTTAACCCAAACTGTCAAGATTTAGGATA
1361 GTCAAGAAAAGGTTTAACTAGTTTTAGTATCAGACTTTAAAATTTCTTAAAGATTTTGGTAAGAAATAGAAGTCCACATA
1441 CAAGCATTGAGGAAACTGAAAAATCTTTGTAAGACCCTTAATGGCTAAAGCCATTGGCTGTAATGAGTGTTACTGTGTGA
1521 GGCTTTGTGTATGAGATCTGTGCTGTAAGGCTCCCAGTGTTCCTTCAGCATTAATGCTTTCCCACCTGCTCCAAGAGAAC
1601 TAGCCCTCAACCAGTGCTGCGGCCTGTTCATATGCTCTCATACTTTTTCAAATCATGATGAGAATTTTTCTCCTATATAC
1681 TAAATCCTCTTAAAGACTCAACTCTTTGTAGTTTTGTCAAAGACAAATACTCTGTAAAGTCCCCCACCTACCCCAGATCT
1761 ACAGCTTTTTAGCTTTTAATTTGAATCATCTGCATAGTTGCTTGGATCATTGTTACATCAGTTCAGCGCATAGTAGCAAT
1841 TCTGGTGTGGCCAAGGTGTTGGCTGAAGTTTATTCTTCATTGGATGTTTAAAGCATTTACTCCATTGTTAAGGTTGGCAT
1921 CATATAGTCAGTTACTGACATGGCATAAGACAGCATGAAACTTTACCACATTTATAAATATGAGGGATTTTTTCTTTTGT
2001 TGGTTTTTAACCCTTTTAGACTAAGCCTCAAAAATATTATCATACCTGTGTACTTGTACATTAAAAACAAAATTATTGCT
2081 GAGTAAGAAGATATTTAATATATGAAACTCTCATAACTGTCATTAACAGTATTAATTAGGTCTAAATACCGTGGAGCAGC
2161 CCTGATATCATGCTGACAGCTTACGTGTCAGAGGGCAGAACTCAGCCAGTCCTGTGGTTTCAGGTGTTAATATGTAAACA
2241 AGCATCTCATTTAACTTCACTGTGCTCAGCTTTGAAAAGAACAAAGATTTATACCTCTGTACATGTGGATAGATAGAAGT
2321 GTCCTGTTTTGATACTAATTTTGAAAGTTGATTATCTTCTCTTGATATCTTGGTTAAAACTGGCCACAAGATAAACCATT
2401 TGGATATATTTGATTTTGTCATAACATTTGTAAAGTATATGTTTCTCATGAGACTGAGGGTTTTATTTTTAAAATCCCCT
2481 TACATTTCTAGCACACCTTGGCCATCACAGTAGACAAAGAATACAGAGAAGGCAGTGGGTTAGGCAGTGGGGGATGGGTT
2561 TTTGGTTTTTTTTTTGTTTTTTTTTGGGTTTTTTTGGTGACATTTTCTCTTAAGAAAGTGCTTTAGCACCGCTGGCTGGC
2641 ATCTTTAATGCAGGTGGTAAACCTAATTCCATAAGCATGGCCTGCCCGAGCCAGAAGGAAGAAGAGCTTGCTCCCTGGCC
2721 TGAGGAAGTGCCTCGTAGGTAGTCATGGAAGTCACAGGTAAGTGCAGGAGCTTGAAGACAGGCAAGAGGTCTCTAAGGTG
2801 TTCCTTTCCTTTGTCTGAGTGTATAGTATTAAGATGCCTAGAAGAGAGATGGTTTTGTTCCACTCTTACAGGCACTTTAT
2881 ATTTCTTGCTGTAACTACAGCAATCACATACAGTACAGACAACCTCAAAAATCTGTTTATATTAGTGTAAATAGAAAAAT
2961 GGTAGACCGCTGTCTAGTTTTCTCCGCAGGCATTTTTGGGAGTGTATCTATAGCCTGTTCTACTTTGTTAATGGGGGATA
3041 ACAGCACATCCTAGATCATGTGTGAACTTTGGGGTTTTTAATTGCCCTTGGTATCCTGATATTCTCATTTTCAAGGTGAG
3121 GTAAAGACAGGGTTTCCTTACTTAACGGATTTGAGCGCTTCTGTTCTCCAAACACAATGGAATTTCTTGTTCAAGAGCAG
3201 CTTCCAGAGTTGAGCCAGCCCTCGCTGCCTTGGGTGCTTGCTTACCACCAGTCAAGGTTGCTCTGAACTTTCCCATAACC
3281 ATTATCCCCCAAGACCACCAGGCTCTGAGTGACCTCTCTAATTCTGTCTTCCCACCCACCAGTGACTTGACTGCTCTGCA
3361 CCTTTGCATCTTCAGAGCTGCTGCGTCTATCAGGAGAGCAGGATCAGATTATGGTATCCAGATACCAGTAGAAGAATTGG
3441 ATGTTATACTATACTTAGCACCTTAATTCTACTTAACATTTACTTCTTAAGCTGACAACTAGATTCAGAAATACAAGAAA
3521 AATAGACTATGTCAGGTCCTCAAGTTATGAGTTAGGACCAACGACTATTAACTTGCATTCACATTATCCTCACAGAACCA
3601 AGAGTGGCAATGCTTTGGAGAATAGCAGTCCAGCCTAGTTAACATGGGGAAAGATGACAACATTGTATGTAGTGTTGAAC
3681 CTGAAGACATCCTACAATGATTTCCCACACTCTATTTTTCCTTATGTTTGCTTAAATAGCAAAAATCAACACTAAATGTC
3761 TAAAGGAATTTGTAATTTGAGACCTAGTATTTTTACACAATAAACCTGCTAAAACAGGCTTTAAAAGACAGCAAAATAAG
3841 CCATATGTAGTGGTGTTAAGACTGTAATCCCAGCACTCAGGAAGCTGAGACAGGAGAATTGCCGGGAGTACAAAGTCAAC
3921 CGGAGCTAAATAATATCAGCCACCAGGACTACATAACGAAAGATTGCCACAGAGGAAAGGAAGGAAGAAAAAGCCAGCAA
4001 TCAACCATCATGTGTTTCTGTAGAATCAGATGTAATAGGAAACATAGTTTCAGATTGCTTCTGACAAAGTCTGTGCCACA
4081 TTCCTCACAGCAGGTGTGACTCTTTAAGACCATGATAGTGGAGTAAGGGAAAGAGTGAGATGTAGCGCTCTGGATGCTTA
4161 AAAAGTAAGTGCTTGGATTTAACCCTAAAGTGGTTTGAAATTGGGAAGAAGTGTGACAGGATACAGTTGGGCTAATTATG
4241 CAATATTTCATCTGTTTAATTTTCTAACTCTGTATTGTTCCTAAAGACGGATGAATGAGTCTCTGTGGTGTTGCCTGCTC
4321 ATTGTGCATACCCTTGGTAGACATTTCTCATAGTGTGTACTTAAAAAGTGCCTGACATTGATTTGTGTTGATTTCTAAGT
4401 CCATGTAATAAATGATACATGTTTTCTATGATA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' uggAGAAACGUCUACUCUGACu 5'
             | ||| |  ||||||||| 
Target 5' ataTGTTT-CTCATGAGACTGa 3'
 2437 - 2457 158.00 -15.01
2 
miRNA  3' uggaGAAACGUCUACUCU-GACu 5'
              |||||:: |||||| ||| 
Target 5' gaggCTTTGTGTATGAGATCTGt 3'
 1519 - 1541 142.00 -17.30
3 
miRNA  3' ugGAGAAACGUCU--ACUCUGACu 5'
            :|| || || :  || ||||| 
Target 5' caTTCCTTCCAAGCCTGTGACTGg 3'
 180 - 203 126.00 -11.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions C2C12
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1385342. RNA binding protein: 聽AGO2. Condition:C2C12_GM_Ago2_CLIP-Seq_myoblast ...

- Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al., 2014, Cell.

Article - Zhang X; Zuo X; Yang B; Li Z; Xue Y; Zhou et al.
- Cell, 2014
MicroRNAs are well known to mediate translational repression and mRNA degradation in the cytoplasm. Various microRNAs have also been detected in membrane-compartmentalized organelles, but the functional significance has remained elusive. Here, we report that miR-1, a microRNA specifically induced during myogenesis, efficiently enters the mitochondria where it unexpectedly stimulates, rather than represses, the translation of specific mitochondrial genome-encoded transcripts. We show that this positive effect requires specific miR:mRNA base-pairing and Ago2, but not its functional partner GW182, which is excluded from the mitochondria. We provide evidence for the direct action of Ago2 in mitochondrial translation by crosslinking immunoprecipitation coupled with deep sequencing (CLIP-seq), functional rescue with mitochondria-targeted Ago2, and selective inhibition of the microRNA machinery in the cytoplasm. These findings unveil a positive function of microRNA in mitochondrial translation and suggest a highly coordinated myogenic program via miR-1-mediated translational stimulation in the mitochondria and repression in the cytoplasm.
LinkOut: [PMID: 25083871]
CLIP-seq Support 1 for dataset GSM4751759
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of iBAT 4
Location of target site NM_182994 | 3UTR | GUUUCUCAUGAGACU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4751763
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 4
Location of target site NM_182994 | 3UTR | GUUUCUCAUGAGACU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM1385342
Method / RBP HITS-CLIP /  AGO2
Cell line / Condition C2C12 / C2C12_GM_Ago2_CLIP-Seq_myoblast
Location of target site NM_182994 | 3UTR | AAGUAUAUGUUUCUCAUGAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 25083871 / GSE57596
CLIP-seq Viewer Link
26 mmu-miR-452-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT429003 Nfib nuclear factor I/B 2 2
MIRT577016 Vmp1 vacuole membrane protein 1 2 8
MIRT580097 Usp54 ubiquitin specific peptidase 54 2 2
MIRT581145 Senp1 SUMO1/sentrin specific peptidase 1 2 2
MIRT581215 Samd4 sterile alpha motif domain containing 4 2 2
MIRT582710 Lcorl ligand dependent nuclear receptor corepressor-like 2 6
MIRT583742 Ephb3 Eph receptor B3 2 10
MIRT583790 Elk4 ELK4, member of ETS oncogene family 2 2
MIRT584103 Csnk1e casein kinase 1, epsilon 2 6
MIRT587976 Armc1 armadillo repeat containing 1 2 2
MIRT590630 Arl5a ADP-ribosylation factor-like 5A 2 2
MIRT591969 Bbx bobby sox HMG box containing 2 2
MIRT591998 Wrn Werner syndrome RecQ like helicase 2 2
MIRT594081 Sema6d sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D 2 2
MIRT594218 Ube2k ubiquitin-conjugating enzyme E2K 2 2
MIRT594859 Nacc1 nucleus accumbens associated 1, BEN and BTB (POZ) domain containing 2 2
MIRT595550 Celf2 CUGBP, Elav-like family member 2 2 2
MIRT595570 Mtf1 metal response element binding transcription factor 1 2 2
MIRT596982 Tango6 transport and golgi organization 6 1 1
MIRT599081 Eid2b EP300 interacting inhibitor of differentiation 2B 2 2
MIRT599176 Dhdh dihydrodiol dehydrogenase (dimeric) 2 2
MIRT600982 Denr density-regulated protein 2 2
MIRT602199 Hlcs holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) 2 2
MIRT603280 Stambp STAM binding protein 2 2
MIRT603947 Grap2 GRB2-related adaptor protein 2 2 2
MIRT606257 Polr1e polymerase (RNA) I polypeptide E 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-452 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-452 Phenethyl isothiocyanate(PEITC) NULL 16741 Microarray neonatal mice liver 20145010 2010 up-regulated
miR-452 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-452 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 down-regulated

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