miRTarBase - #MIRT591253


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pre-miRNA Information
pre-miRNA mmu-mir-674   
Genomic Coordinates chr2: 117185127 - 117185226
Synonyms Mirn674, mmu-mir-674, Mir674
Description Mus musculus miR-674 stem-loop
Comment Landgraf et al. show that the 5' miRNA product is the predominant one .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-674-5p
Sequence 25| GCACUGAGAUGGGAGUGGUGUA |46
Evidence Experimental
Experiments MPSS
Putative Targets

Gene Information
Gene Symbol Loxl2   
Synonyms 1110004B06Rik, 4930526G11Rik, 9430067E15Rik
Description lysyl oxidase-like 2
Transcript NM_033325   
Expression
Putative miRNA Targets on Loxl2
3'UTR of Loxl2
(miRNA target sites are highlighted)		 
>Loxl2|NM_033325|3'UTR
   1 AGAAGATCCTGGGCCAGGCATGATGGCTCATGCCTGTAATCCCTGCACTCATGCTGAGGCAGGAGGATTGCCACAAGATT
  81 TCCACTCTGGACATTAAACCAAGCTTCAGTTTCAAAAGAAATGAATGAAAGAAAGGAAGGAAGGAAGGAAGGAAGGAAGG
 161 AAGGAAGGAAGGAAGGAAGGAAGAAAGGGGAAAGGGAAAGGGAAGAAAAATGACTTAATGGTCACTTACTGACTCCTGGG
 241 GGAATACTGATTACCACCTCTTTTCTAGCCAGATCCAGCTGAGAAGAAAGGTGCTCATTCACTCCCCAGACACTGCCGTG
 321 TGTCCCTGTCCTGAGGCCTTAGGGGCAGGGCTCGGGCACATGGCCATGGAAACTTGATGACAAGCTTAGAGCAGCTTATC
 401 CCATCCGAGCTTTGGCATGTCCCAAGTGTGACATCATCTGTGCTCTGCACAGAGGGGCCGTTTTCTTCTGGGAACACAGC
 481 AGGCATGAACTCAGCAACTGCAGAGGTGATCGGGCTGAACTCCGTTTTTCCCCTTCTTAGGTCATTTCTGGAAAACTTGA
 561 ATATCAAGACCTCTGTATTAAGTTTGTTTGGTTTTTTGTTTTGTTTTGTTTTGTTTTTTCTTCTCATTGTACAAACTGCT
 641 TGGCAAAATCAACTTGAAGAAGGAAGGCTTTATGTGAGCTCGTGGTTTGAGGAACCAATCTGTAGTGGCAAAGAAAGTAT
 721 GACAGCAGGAGCGTGAGGTGTCTGGTTCCAAAACTCAGTTATGGAGCTGAGGATGACCTTGGACCTCTGAGCCTCCCTCC
 801 CGCTTTCAACTCCAGAGTGCTGGGATCAAACCCAGGACTGCACACTTGTAAGGCAACATGCTCTCTATCGGTTGTAGCTG
 881 CTTTCTGAGCACTTACCCTTCTCTGCTGCTCTAAACCTGTACGTGATCTTTGCCTTACCGATCCGCACATTTTTCTGTCT
 961 CTGTGCTCCACTTGCTCATTGTCACTGTGGGTCTGAGTAAGAGCAGTGACCAGAAATGCATAGCACAGCCTGAATGCTAC
1041 CCTGTCTTAAAATGTTCTCTGCCAAGCAAATGTGTCCATTACTCTTGAGTTAAGACTCACTCGAGTGCTCAGAACACAGG
1121 CAGGATGCAGCTGGATTCTTGCCAGGGTGTAACCTGATTGGCTTCTAGCTCAGTTTCAGATAGATAGTCCTTGGTTACCA
1201 CTAAAACCTCAGGTACCAGGCCTCCACTCTCCACGTTCCACTTGGCGCTCTGGTCTTCCAAACTCCCACCACAATAACCC
1281 ATTCAGTTCTGTTCTCTAAAAGCGCTCTAGGGCTTCTGGACCCAAAGTTCTAAGTTCTTCCAGATTCTCCCCCCAAGCCA
1361 ACCCCCAAAGCTCAAGAACTCCACGACCATGCTTCTGACCTCAGTGATCAGTGACAGCTCTAGTCTTCTGTATTATACTT
1441 GACAAAAACAACTTAAGGGAAGGCTACTTTTGGCTTACAGTTCGAGAGTTCAGTCCCTCACAGCGGGTAAAGCATGGCGG
1521 CAGGAGCAGCTGGTCATGTGGCATCCACAGTCAGGAAGCAGAATGACGAATGCAGGTGCTCAACTTGCTCTCACCTTTAT
1601 ATTCAGCCTACAACCCCAGCTCATGGAATGGTGCTACTGGTGTCTAGGGTGGGCCTTCCCTCTTGTGTTAAACCTCTTCT
1681 GGAGACACCCCTATAGACCCACCCAGGGGTGTTTCCATAGTGGTTCTAAATCCCAGCTAGCTGATAATCAAGGTTATCTA
1761 TAACAACCTCTTGCAGTTAAACCCACGTCTGTATTCCATGCAATTATAGCCACGACTTAAAGTACAATAGCAAGGCATAC
1841 ATCCCAAGGCTCCAAGGGATGGCTGAAGACTTGGATGGTTCTGGGACAGGATAGGAAGAGAAAGTCATGGGGGAACAACA
1921 TGCAAGGGGCTCTTCACCTCAGAGCCTGGCTTATGCCAGCTGAGACTCCCAACACAGATAGCATTGTGAATGGTGCTAAT
2001 ATAGGGTCAGTATTCATTTTGCATATTTATATACAATTATAGATATACAAGTATAGATATTTCAAGTAAGAATATTAAAA
2081 TAGCAAGGAATGATGATTAAGGCTGAAGTGTAAATGGCCTTTTTCCTTTCCTGGCTGTAGACATACAATTCCCTACAACT
2161 TTGCCAGAAACCCCACTTACTAAAAGGCATATCTCAAGACCCCTATGCTGGCCCAATGTTTTCATCTTATCAGGGCGCAG
2241 TCCTATCCAAGGTACCTCTGGAGCCACCTTATAATCTTACTATCAAAAGGGGGAAGGGGGGGTGGGACTTAGAAACCAAT
2321 CGAGCAACACCAAAGGCTTCGGGACTCCTGTGTGTAGCTAGTTATTAAGACACTTAAGAACTCTCGTCCTGGGGCTGGTG
2401 AGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCTAGAGTTCAAATCCCAGCAACCACATGGTGGCTCA
2481 CAACCATCTGTAATGAGATCTGACTCCCTCTTCTGGTGTGTCTGAAGACAGCTACAGTGTACTTACATATAATAAATAAA
2561 TAAATCTTTAAAAAAAAAAAAAGAACTCTCGTCCTTGGAGAAGCCCACAGCCCAGCTCTCTGGTGTGTCTTACTCTTTCC
2641 CTGAGCACCTCTTAACCCCTGTAACTTAAATTTATATTTAAAATATTTTAAATAAACGTTTAACTCTCTGCTTTAAAAAA
2721 A
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' augUGGU--GAGGG-UAGAGUCACg 5'
             ||||  ||:|: | ||||||| 
Target 5' acgACCATGCTTCTGACCTCAGTGa 3'
 1383 - 1407 161.00 -17.60
2 
miRNA  3' augUGGUGAGGGUA--GAGUCACg 5'
             :|:: |   ||  ||||||| 
Target 5' gggGCTGGTGAGATGGCTCAGTGg 3'
 2391 - 2414 141.00 -10.50
3 
miRNA  3' auguggUGAGGGUAGAGUCAcg 5'
                :||:|:| ||||||  
Target 5' tgattgGCTTCTAGCTCAGTtt 3'
 1155 - 1176 140.00 -13.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1 HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' augUGGUGAGGGUA--GAGUCacg 5'
             :|:: |   ||  |||||   
Target 5' gggGCUGGUGAGAUGGCUCAG--- 3'
 		3 - 23
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Liver
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in ERR266300. RNA binding protein: AGO2. Condition:B_Untreated ...

- Schug J; McKenna LB; Walton G; Hand N; et al., 2013, BMC genomics.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' augUGGUGAGGGUA--GAGUCACg 5'
             :|:: |   ||  ||||||| 
Target 5' gggGCUGGUGAGAUGGCUCAGUGg 3'
 		3 - 26
Article - Schug J; McKenna LB; Walton G; Hand N; et al.
- BMC genomics, 2013
BACKGROUND: Validation of physiologic miRNA targets has been met with significant challenges. We employed HITS-CLIP to identify which miRNAs participate in liver regeneration, and to identify their target mRNAs. RESULTS: miRNA recruitment to the RISC is highly dynamic, changing more than five-fold for several miRNAs. miRNA recruitment to the RISC did not correlate with changes in overall miRNA expression for these dynamically recruited miRNAs, emphasizing the necessity to determine miRNA recruitment to the RISC in order to fully assess the impact of miRNA regulation. We incorporated RNA-seq quantification of total mRNA to identify expression-weighted Ago footprints, and developed a microRNA regulatory element (MRE) prediction algorithm that represents a greater than 20-fold refinement over computational methods alone. These high confidence MREs were used to generate candidate 'competing endogenous RNA' (ceRNA) networks. CONCLUSION: HITS-CLIP analysis provide novel insights into global miRNA:mRNA relationships in the regenerating liver.
LinkOut: [PMID: 23597149]
94 mmu-miR-674-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577119 Prps2 phosphoribosyl pyrophosphate synthetase 2 1 3
MIRT579212 Ccdc25 coiled-coil domain containing 25 1 4
MIRT580735 Srp72 signal recognition particle 72 1 2
MIRT581673 Ppp2r2c protein phosphatase 2, regulatory subunit B, gamma 1 1
MIRT582930 Il15ra interleukin 15 receptor, alpha chain 1 1
MIRT584530 Bzrap1 TSPO associated protein 1 1 1
MIRT584648 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 1 1
MIRT586666 Mbp myelin basic protein 1 2
MIRT588993 Rpl7l1 ribosomal protein L7-like 1 1 1
MIRT589588 Magt1 magnesium transporter 1 1 1
MIRT590893 Tmem69 transmembrane protein 69 1 2
MIRT590965 Slfn5 schlafen 5 1 2
MIRT591089 Pola2 polymerase (DNA directed), alpha 2 1 2
MIRT591253 Loxl2 lysyl oxidase-like 2 1 2
MIRT591405 Churc1 churchill domain containing 1 1 2
MIRT591425 Casc4 cancer susceptibility candidate 4 1 2
MIRT591686 Slc23a1 solute carrier family 23 (nucleobase transporters), member 1 1 2
MIRT591985 A230046K03Rik WASH complex subunit 4 1 2
MIRT593044 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1 2
MIRT593330 Ddx3x DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked 1 1
MIRT594701 Anks1b ankyrin repeat and sterile alpha motif domain containing 1B 1 1
MIRT595566 Sh3rf3 SH3 domain containing ring finger 3 1 1
MIRT595693 Pcsk6 proprotein convertase subtilisin/kexin type 6 1 1
MIRT595863 Myh10 myosin, heavy polypeptide 10, non-muscle 1 1
MIRT596037 Ubp1 upstream binding protein 1 1 1
MIRT596075 Ywhag tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 1 1
MIRT596182 Med22 mediator complex subunit 22 1 1
MIRT596482 Zyg11b zyg-ll family member B, cell cycle regulator 1 1
MIRT596495 Zfp935 zinc finger protein 935 1 1
MIRT596811 Tyk2 tyrosine kinase 2 1 1
MIRT596873 Tspan15 tetraspanin 15 1 1
MIRT597076 Tbc1d24 TBC1 domain family, member 24 1 1
MIRT597180 Spc24 SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) 1 1
MIRT597235 Slc7a14 solute carrier family 7 (cationic amino acid transporter, y+ system), member 14 1 1
MIRT597281 Slc22a15 solute carrier family 22 (organic anion/cation transporter), member 15 1 1
MIRT597347 Scd3 stearoyl-coenzyme A desaturase 3 1 1
MIRT597359 Sap18 Sin3-associated polypeptide 18 1 1
MIRT597446 Rin1 Ras and Rab interactor 1 1 1
MIRT597514 Rbbp4 retinoblastoma binding protein 4, chromatin remodeling factor 1 1
MIRT597557 Rab33b RAB33B, member RAS oncogene family 1 1
MIRT597808 Pla2g4b phospholipase A2, group IVB (cytosolic) 1 1
MIRT597974 Nup133 nucleoporin 133 1 1
MIRT598205 Mpv17l Mpv17 transgene, kidney disease mutant-like 1 1
MIRT598257 Mfsd2b major facilitator superfamily domain containing 2B 1 1
MIRT598574 Irak1 interleukin-1 receptor-associated kinase 1 1 1
MIRT598860 Gm10778 predicted gene 10778 1 1
MIRT599029 Fam177a family with sequence similarity 177, member A 1 1
MIRT599181 Dennd5b DENN/MADD domain containing 5B 1 1
MIRT599224 Dcaf4 DDB1 and CUL4 associated factor 4 1 1
MIRT599261 D3Ertd254e DNA segment, Chr 3, ERATO Doi 254, expressed 1 1
MIRT599278 Cyp2e1 cytochrome P450, family 2, subfamily e, polypeptide 1 1 1
MIRT599286 Cxcr2 chemokine (C-X-C motif) receptor 2 1 1
MIRT599294 Cx3cr1 chemokine (C-X3-C motif) receptor 1 1 1
MIRT599328 Comt catechol-O-methyltransferase 1 1
MIRT599355 Commd7 COMM domain containing 7 1 1
MIRT599488 Ccr9 chemokine (C-C motif) receptor 9 1 1
MIRT599583 Brip1 BRCA1 interacting protein C-terminal helicase 1 1 1
MIRT599647 Banp BTG3 associated nuclear protein 1 1
MIRT599683 Arl16 ADP-ribosylation factor-like 16 1 1
MIRT599726 Ankfy1 ankyrin repeat and FYVE domain containing 1 1 1
MIRT600098 1700047I17Rik2 RIKEN cDNA 1700047I17 gene 2 1 1
MIRT601167 Atrn attractin 1 1
MIRT601187 Asxl2 additional sex combs like 2 (Drosophila) 1 1
MIRT601347 Zfp202 zinc finger protein 202 1 1
MIRT601378 Wdr89 WD repeat domain 89 1 1
MIRT601554 Tapbpl TAP binding protein-like 1 1
MIRT601574 Ssr1 signal sequence receptor, alpha 1 1
MIRT601739 Rbm34 RNA binding motif protein 34 1 1
MIRT601844 Polr2l polymerase (RNA) II (DNA directed) polypeptide L 1 1
MIRT601894 Pbld1 phenazine biosynthesis-like protein domain containing 1 1 1
MIRT601949 Nol3 nucleolar protein 3 (apoptosis repressor with CARD domain) 1 1
MIRT602826 AK010878 GON7, KEOPS complex subunit homolog 1 1
MIRT602859 Rmi2 RecQ mediated genome instability 2 1 1
MIRT602916 1700016D06Rik RIKEN cDNA 1700016D06 gene 1 1
MIRT603001 Myo1c myosin IC 1 1
MIRT603576 Ppm1k protein phosphatase 1K (PP2C domain containing) 1 1
MIRT603581 Ppil4 peptidylprolyl isomerase (cyclophilin)-like 4 1 1
MIRT603702 Nip7 NIP7, nucleolar pre-rRNA processing protein 1 1
MIRT603710 Naip5 NLR family, apoptosis inhibitory protein 5 1 1
MIRT603942 H2-T23 histocompatibility 2, T region locus 23 1 1
MIRT604103 Dph5 diphthamide biosynthesis 5 1 1
MIRT604202 Cpne9 copine family member IX 1 1
MIRT604229 Clca2 chloride channel accessory 3A2 1 1
MIRT604242 Cflar CASP8 and FADD-like apoptosis regulator 1 1
MIRT604392 Aipl1 aryl hydrocarbon receptor-interacting protein-like 1 1 1
MIRT604542 Ypel2 yippee-like 2 (Drosophila) 1 1
MIRT605321 Zfp605 zinc finger protein 605 1 1
MIRT605421 T2 brachyury 2 1 1
MIRT605424 Synrg synergin, gamma 1 1
MIRT605630 Mfsd11 major facilitator superfamily domain containing 11 1 1
MIRT605750 Exosc2 exosome component 2 1 1
MIRT605832 Cenpf centromere protein F 1 1
MIRT606053 Zfp607 zinc finger protein 607A 1 1
MIRT606349 Lcmt2 leucine carboxyl methyltransferase 2 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-674 Glucose NULL 5793 Quantitative real-time PCR endothelial cells 24394957 2014 down-regulated
miR-674 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated

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