miRTarBase - #MIRT593069


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pre-miRNA Information
pre-miRNA mmu-mir-326   
Genomic Coordinates chr7: 99552269 - 99552363
Synonyms Mirn326, mmu-mir-326, Mir326
Description Mus musculus miR-326 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-326-3p
Sequence 60| CCUCUGGGCCCUUCCUCCAGU |80
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Ccdc25   
Synonyms 2610528H13Rik, NSrp70
Description coiled-coil domain containing 25
Transcript NM_145944   
Expression
Putative miRNA Targets on Ccdc25
3'UTR of Ccdc25
(miRNA target sites are highlighted)		 
>Ccdc25|NM_145944|3'UTR
   1 GCTGAGCAACGGACCCTGGGAAGACATGAATGTGCAGACCATTGCAGAGCTTTTGATTTCATCTAGGTTCTGAATTACAG
  81 AAACCAACCAACCAAAATTCTACCTTCATTCTACATAGACATCATTGGTTTTGGAGTTTAAAAAAAAAAGTCACCTTTTT
 161 GCTGATGAAAAGAACATATGTATAATATAGTTGAACTCGAAGACAACTTAAAGTTTAGGGAAAGGAGAATATTTTTGGCA
 241 GAACATCCGTCAGTACCTCATGAAGGCTGGCTGCCTTTGTCTTTGAGATAGACATTGGAGCAGACGGACTCTGGAAAGTG
 321 CTTCTGTGGCTGTGGTCTGTCCTGGGAACAGACGTGGTAAAATGCGTTTCCTATCTCTAAAATATCTACTTCTGTGAAGA
 401 AGACTGGCTTACTCAGGCCAGGGCCCTCCTGACGACGGTTTCCTGACTAGAAGAAAGGGACGTGGGTTTGAAGGCAGCAG
 481 CAGTGCTTACTTAACCTGCATTTCAGCATTTGGCAAGTTCTCTCTTGCTGGCGTCAGAGTAGCTGGCCCAGCTCTTAGCT
 561 TGTTCTGAGGGTTCTGCTGTGAGGTGAGGGCCCCATCTCAGCATCGTAGTTACATAGTTGATCTTAACTAAGCTTGGGAA
 641 TACGTCACCTGAGATCACAGGTGATCCAAGCCGCAGATGTGACAGCTGGGCTCTGAAGCTATTTCTGTGTGTGGAGGGAG
 721 ACAGGAAAGGGTGCTGTGCTAGTAGTGAGAGTGACACAGATTGCCTCATGATTGTCTGGAGTGTATCATGAAACAAAAGT
 801 CCACCATGGATTGCAAGCTTTAGAACGAATCAAGCATTTTCCCTAGCCAGGCAAGGCATTCGTTTCCTTACTTTGGAATA
 881 GTATTTATGTTGAATTGTAAACCCACAGTTGTAAGCTTAGTGACTTCACATGTATGTAGCATTGGTACTGGTCTGAGCCA
 961 CCTGCTACCCAGTAGATAGATAACCTTTTTGTTAGTGTTTAAGGGAGCCCCTGAAGAGACTCAACAGAACAGTGAGAGGA
1041 GAGAGAGAAAAGCTGGCAGGGTTGTGACTGGCATGCAAATCCATATTCTAGCACACCACACAGTGCCCACAGCTCTGCGG
1121 TGCTGAGCCAGCAGAAATGATTTCTTCAGCAGATGTACCAGCTGTCATCACTGACGTATGCACAAAACCAGACCGTTGAT
1201 CCCTGGTATTCCAAGTTGAGTATGGGTTGAACTGGAAAATTCCCTGAGGCATAACTTGTTAGCCAATTTGAGGAGAGCCA
1281 GTTTGAGGGTTATGAAAGTCAGCACCAGAGATCAGTGAGTGTGAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
1361 GTGTGTGTGTGTGTGTATGTGTGTGATAGTTAGACTGGAGTTCTGTGATTTAAAAAAAATTATAATAAAGTATCATTTTT
1441 GTCAG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugaccuccuUCCCGGGUCUCc 5'
                   || ||||| || 
Target 5' acaccacacAGTGCCCACAGc 3'
 1093 - 1113 120.00 -8.50
2 
miRNA  3' ugaccuccuucccgGGUCUCc 5'
                        |||||| 
Target 5' tatgaaagtcagcaCCAGAGa 3'
 1291 - 1311 120.00 -10.30
3 
miRNA  3' ugACCUCCUUCCCGGGUCUCc 5'
            ||||  ||   ||| ||| 
Target 5' acTGGA--AAATTCCCTGAGg 3'
 1231 - 1249 119.00 -18.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Liver
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in ERR266293. RNA binding protein: AGO2. Condition:A_Liver partial hapatectomy 36h ...

- Schug J; McKenna LB; Walton G; Hand N; et al., 2013, BMC genomics.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ugaccuccuucccgGGUCUCc 5'
                        |||||| 
Target 5' -----------gcaCCAGAGa 3'
 		1 - 10
Article - Schug J; McKenna LB; Walton G; Hand N; et al.
- BMC genomics, 2013
BACKGROUND: Validation of physiologic miRNA targets has been met with significant challenges. We employed HITS-CLIP to identify which miRNAs participate in liver regeneration, and to identify their target mRNAs. RESULTS: miRNA recruitment to the RISC is highly dynamic, changing more than five-fold for several miRNAs. miRNA recruitment to the RISC did not correlate with changes in overall miRNA expression for these dynamically recruited miRNAs, emphasizing the necessity to determine miRNA recruitment to the RISC in order to fully assess the impact of miRNA regulation. We incorporated RNA-seq quantification of total mRNA to identify expression-weighted Ago footprints, and developed a microRNA regulatory element (MRE) prediction algorithm that represents a greater than 20-fold refinement over computational methods alone. These high confidence MREs were used to generate candidate 'competing endogenous RNA' (ceRNA) networks. CONCLUSION: HITS-CLIP analysis provide novel insights into global miRNA:mRNA relationships in the regenerating liver.
LinkOut: [PMID: 23597149]
23 mmu-miR-326-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004432 Smo smoothened, frizzled class receptor 3 1
MIRT005596 Ets1 E26 avian leukemia oncogene 1, 5' domain 3 1
MIRT581306 Rnf150 ring finger protein 150 1 2
MIRT582546 Map3k7 mitogen-activated protein kinase kinase kinase 7 1 1
MIRT582559 Maff v-maf musculoaponeurotic fibrosarcoma oncogene family, protein F (avian) 1 1
MIRT582898 Ildr2 immunoglobulin-like domain containing receptor 2 1 1
MIRT583744 Ephb3 Eph receptor B3 1 5
MIRT590217 Dync1li2 dynein, cytoplasmic 1 light intermediate chain 2 1 2
MIRT590852 Vsx1 visual system homeobox 1 1 2
MIRT593069 Ccdc25 coiled-coil domain containing 25 1 1
MIRT596778 Usp22 ubiquitin specific peptidase 22 1 1
MIRT596972 Tmem179 transmembrane protein 179 1 1
MIRT597063 Tbx5 T-box 5 1 1
MIRT597081 Tars2 threonyl-tRNA synthetase 2, mitochondrial (putative) 1 1
MIRT598144 Mylip myosin regulatory light chain interacting protein 1 1
MIRT600897 Fbxl16 F-box and leucine-rich repeat protein 16 1 1
MIRT600919 Exoc8 exocyst complex component 8 1 1
MIRT604218 Clgn calmegin 1 1
MIRT604799 Phactr1 phosphatase and actin regulator 1 1 1
MIRT605009 Ept1 selenoprotein I 1 1
MIRT605050 Efr3b EFR3 homolog B 1 1
MIRT755982 Th tyrosine hydroxylase 3 1
MIRT756011 Tlr4 toll-like receptor 4 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-326 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-326 Propranolol approved 4946 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-326 Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 up-regulated
miR-326 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 down-regulated
miR-326 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated

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