miRTarBase - #MIRT593891


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pre-miRNA Information
pre-miRNA mmu-mir-743b   
Genomic Coordinates chrX: 66777256 - 66777332
Description Mus musculus miR-743b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-743b-3p
Sequence 46| GAAAGACAUCAUGCUGAAUAGA |67
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Elovl5   
Synonyms 1110059L23Rik, AI747313, AU043003, HELO1
Description ELOVL family member 5, elongation of long chain fatty acids (yeast)
Transcript NM_134255   
Expression
Putative miRNA Targets on Elovl5
3'UTR of Elovl5
(miRNA target sites are highlighted)		 
>Elovl5|NM_134255|3'UTR
   1 CAAGCCGAGCCGAAGCCTCCAGACCGCGGCGTGTGATTGTAAGCACAGCGTGACTTGTGCCCTGACGCTCATAGCAGCTG
  81 CTGTCACTAGTCTGGCCCTACTATCTGCAGTGTGACTCATGTAGGACCTTCTTCATCAGTTCAAAACCCCTAGAATATGT
 161 ATACAGATTATACAAAGTAGGCATACGATTTTCTCACATTTCTGGGCTCTCATCGACCCCCGCGCTAGACTGTGGAAAGG
 241 GAATGTTCTTGTAGTATACAACACTGCTGTTGCCTTATTAGTTGTAACACGATAGGTGCTGAATTGTGGTTCACAATTTA
 321 AAACACTGTAATCCAAACTTTTTTTTTTTAACTGTAGACCATGCATGTGATTGTAAATTTGTACAATGTTGTTAACAGTA
 401 GAAAAACACACATGCCTTAAGACTTAAAAAAGCAGGGCCCAGAGCTTGTTAGTTTAATTAGGGTATGTTTCAACTTTGTA
 481 TTATTCATTTGTATTAGCTCTGTTTAGAAAAATCAAATGTATGATTTATGAAACAAGTGTGTGATGTGTAACTTCAAGTG
 561 ACTTGGAGATGCGAAATCTGATGGCACCCTTGGTTTTATAACGATGGCTTTAAATAAGCAGGTTCAAGTCTAGTGGAACA
 641 GTCAGTTTAACTTTTTAACAGATCTTATTTTTTTATTTTGAGTGCCACTATTAATGTGTTTAAAGAGGTGGGGAAAGGGT
 721 CCAAAGCGTCTGGAGACACTTACGTACCTGTCCCTGGTCCCCCAGAGGCTAGGGTGACTATAAGAGTAGGTCACTTTTCA
 801 CTTCTGAAGTGCTTAGTGTTTTTATACAGGAGATAAAAAGGCCATTCGACTTCCCACCACTTGGAGAGAGAGAACTACCT
 881 TTCAGGAAAGGTGACGAGACATTTATTATCTGACAGACACACACACACACACACACAGTGATAAGGGACTGGTTACCTGG
 961 GTCCACATTTTTTTTTATTCTTTTCTGTAGTGCTACTTTGACATACACTTCCTTTTAAAATGTGCCTAATTTACCAAGAG
1041 ACCAGGAAGCACCATAAGCATTTTGAGGTAAAATTTAAGAGCTGGAGCGCGCAGGAACTGGGTGTTTGGGAGCAACCGCT
1121 TTTTTCAGTGTGAATCATGGTGGCATCTGTAATTTTTTTCTGATTAGCACCTAAAGATCGGTTTAAAGTGCTTGGACTGC
1201 TTATCCTAAAATCGTGTCTAGTGCGCTCGAGGCATCGGTCATACAAATGGGGACTCTTTTCATGTGTGTCATTTTTAAAG
1281 TGTTGAAAGCTTAGCCTTTGAATACCTTCTGCATTGGTAAAAAATCCATGGAACCACTCATGGCACATCTTAGGAGATGA
1361 TTGACAATGTATAAACTAATTGTGGGTTTGATATTTATGTAAATACCAGTTTACCATGCTTTAATTTTGCACATTCATAT
1441 TACAAGGAGCCAGTTGGTCCTAATTAGCCTTGTGGGTTTTCTGTTTGGAAGGAGTTGCGGCATCTGTTTACATTTCCCTT
1521 GTCAGATCTAGAATGTGTGTATGTGTGTGTCTTCTCCCTAGGTACCCGTCTGCAGGTGTCTTTACATATCTCAATACAAA
1601 AATTATAACATTTGATAGTGTGCTGTCAAAGTGTGCTTAGCTCATCTGGATATACCCACACTGTTAAATAATGTCTAAAC
1681 ATTAATCATTAAAACCTTTTTGATTACCCGTAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agauaAGUCGUACUACAGAAAg 5'
               || ||| |:||||||| 
Target 5' acccgTCTGCA-GGTGTCTTTa 3'
 1564 - 1584 160.00 -14.90
2 
miRNA  3' agAUAAGU-CGUACUACAGAAag 5'
            |:| :| |::|| ||||||  
Target 5' tgTGTGTATGTGTG-TGTCTTct 3'
 1534 - 1555 135.00 -8.30
3 
miRNA  3' agauaaguCGUACUACAGAAAg 5'
                  || | :|||:||| 
Target 5' tctgaagtGCTTAGTGTTTTTa 3'
 803 - 824 134.00 -7.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0004840
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013593. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013598. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' agauaaGUCGUACUACAGAAAg 5'
                | ||| |:||||||| 
Target 5' ------CUGCA-GGUGUCUUUa 3'
 		1 - 15
Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
CLIP-seq Support 1 for dataset GSM1013593
Method / RBP HITS-CLIP / AGO2
Cell line / Condition CD4+ T cells (C57BL/6) / CD4+ T cells, WT, biological rep7
Location of target site NM_134255 | 3UTR | CUGCAGGUGUCUUUAC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23142080 / GSE41285
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1013598
Method / RBP HITS-CLIP / AGO2
Cell line / Condition CD4+ T cells (C57BL/6) / CD4+ T cells, WT, biological rep12
Location of target site NM_134255 | 3UTR | CUGCAGGUGUCUUUAC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23142080 / GSE41285
CLIP-seq Viewer Link
101 mmu-miR-743b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577961 Pm20d2 peptidase M20 domain containing 2 2 2
MIRT578387 Klra2 killer cell lectin-like receptor, subfamily A, member 2 2 6
MIRT579831 Zfhx3 zinc finger homeobox 3 2 8
MIRT580616 Syp synaptophysin 2 2
MIRT580731 Srrm4 serine/arginine repetitive matrix 4 2 2
MIRT581440 Rasa2 RAS p21 protein activator 2 2 2
MIRT582031 Pappa pregnancy-associated plasma protein A 2 2
MIRT582678 Lin28b lin-28 homolog B (C. elegans) 2 8
MIRT582831 Itih5 inter-alpha (globulin) inhibitor H5 2 2
MIRT583084 Hlcs holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) 2 8
MIRT583879 Dusp18 dual specificity phosphatase 18 2 6
MIRT583900 Dstyk dual serine/threonine and tyrosine protein kinase 2 4
MIRT585980 Sfxn4 sideroflexin 4 2 4
MIRT590345 Cntn2 contactin 2 2 6
MIRT591765 Ppm1f protein phosphatase 1F (PP2C domain containing) 2 4
MIRT592938 Astn2 astrotactin 2 2 2
MIRT593579 Zfp709 zinc finger protein 709 2 2
MIRT593675 Slc5a3 solute carrier family 5 (inositol transporters), member 3 2 2
MIRT593750 Peg10 paternally expressed 10 2 2
MIRT593771 Nmnat3 nicotinamide nucleotide adenylyltransferase 3 2 2
MIRT593825 Krit1 KRIT1, ankyrin repeat containing 2 2
MIRT593859 Gm5460 predicted gene 5460 2 2
MIRT593871 Galnt10 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 2 2
MIRT593891 Elovl5 ELOVL family member 5, elongation of long chain fatty acids (yeast) 2 2
MIRT593935 Cxcl3 chemokine (C-X-C motif) ligand 3 2 2
MIRT593937 Cttnbp2nl CTTNBP2 N-terminal like 2 2
MIRT594001 Aptx aprataxin 2 2
MIRT594019 Tmem241 transmembrane protein 241 1 1
MIRT594730 Zfp931 zinc finger protein 931 2 2
MIRT594766 Tmlhe trimethyllysine hydroxylase, epsilon 2 2
MIRT594836 Opa3 optic atrophy 3 2 2
MIRT594873 Magt1 magnesium transporter 1 2 2
MIRT594927 Gm14326 predicted gene 14326 2 2
MIRT594941 Fosl2 fos-like antigen 2 2 2
MIRT594980 Eda2r ectodysplasin A2 receptor 2 2
MIRT595014 Cpd carboxypeptidase D 2 2
MIRT595076 Yae1d1 Yae1 domain containing 1 1 1
MIRT595132 Lrrc4c leucine rich repeat containing 4C 2 2
MIRT595148 Epha7 Eph receptor A7 2 2
MIRT595169 Zpbp zona pellucida binding protein 2 2
MIRT595180 Tsn translin 2 2
MIRT595185 Trim25 tripartite motif-containing 25 2 2
MIRT595187 Top2a topoisomerase (DNA) II alpha 2 2
MIRT595193 Tmtc3 transmembrane and tetratricopeptide repeat containing 3 2 2
MIRT595199 Tbc1d2b TBC1 domain family, member 2B 2 2
MIRT595204 St6gal1 beta galactoside alpha 2,6 sialyltransferase 1 2 2
MIRT595210 Snx27 sorting nexin family member 27 2 2
MIRT595226 Serinc1 serine incorporator 1 2 2
MIRT595229 Sema4d sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D 2 2
MIRT595232 Sec24a Sec24 related gene family, member A (S. cerevisiae) 2 2
MIRT595235 Scn3a sodium channel, voltage-gated, type III, alpha 2 2
MIRT595242 Rc3h1 RING CCCH (C3H) domains 1 2 2
MIRT595255 Ppm1k protein phosphatase 1K (PP2C domain containing) 2 2
MIRT595259 Plxna4 plexin A4 2 2
MIRT595264 Phf3 PHD finger protein 3 2 2
MIRT595271 Nxph1 neurexophilin 1 2 2
MIRT595273 Npas3 neuronal PAS domain protein 3 2 2
MIRT595298 Kcnv1 potassium channel, subfamily V, member 1 2 2
MIRT595317 Gna14 guanine nucleotide binding protein, alpha 14 2 2
MIRT595321 Gm10318 predicted gene 10318 2 2
MIRT595328 Galnt4 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 2 2
MIRT595347 Frem2 Fras1 related extracellular matrix protein 2 2 2
MIRT595375 Epb4.1l4a erythrocyte membrane protein band 4.1 like 4a 2 2
MIRT595380 Dusp11 dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) 2 2
MIRT595383 Dek DEK oncogene (DNA binding) 2 2
MIRT595385 Dcun1d1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) 2 2
MIRT595391 Cetn3 centrin 3 2 2
MIRT595394 Cd8a CD8 antigen, alpha chain 2 2
MIRT595398 Asprv1 aspartic peptidase, retroviral-like 1 2 2
MIRT595403 Arid2 AT rich interactive domain 2 (ARID, RFX-like) 2 2
MIRT595414 Ankrd16 ankyrin repeat domain 16 2 2
MIRT595416 Anapc11 anaphase promoting complex subunit 11 2 2
MIRT595421 Akna AT-hook transcription factor 2 2
MIRT595438 9930013L23Rik cell migration inducing protein, hyaluronan binding 2 2
MIRT595441 9030617O03Rik D-glutamate cyclase 2 2
MIRT595459 Zswim6 zinc finger SWIM-type containing 6 2 2
MIRT595465 Tspan13 tetraspanin 13 2 2
MIRT595467 Tmem41b transmembrane protein 41B 2 2
MIRT595472 Sv2b synaptic vesicle glycoprotein 2 b 2 2
MIRT595475 Sgk3 serum/glucocorticoid regulated kinase 3 2 2
MIRT595480 Scaf11 SR-related CTD-associated factor 11 2 2
MIRT595485 Phox2b paired-like homeobox 2b 2 2
MIRT595490 Pgp phosphoglycolate phosphatase 2 2
MIRT595494 Nr1d2 nuclear receptor subfamily 1, group D, member 2 2 2
MIRT595511 Lrig1 leucine-rich repeats and immunoglobulin-like domains 1 2 2
MIRT595524 Grm2 glutamate receptor, metabotropic 2 2 2
MIRT595528 Foxn3 forkhead box N3 2 2
MIRT595536 Fign fidgetin 2 2
MIRT595546 Celf2 CUGBP, Elav-like family member 2 2 2
MIRT595553 Cd28 CD28 antigen 2 2
MIRT595840 Sumf1 sulfatase modifying factor 1 2 2
MIRT595953 Rab6a RAB6A, member RAS oncogene family 1 1
MIRT598445 Lix1 limb and CNS expressed 1 2 2
MIRT600750 Iws1 IWS1, SUPT6 interacting protein 2 2
MIRT601150 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 2 2
MIRT602248 Grm1 glutamate receptor, metabotropic 1 2 2
MIRT603333 Slc35d2 solute carrier family 35, member D2 2 2
MIRT603399 Serpina1a serine (or cysteine) peptidase inhibitor, clade A, member 1A 2 2
MIRT604755 Rad51d RAD51 paralog D 1 1
MIRT605865 Atpbd4 diphthamine biosynthesis 6 2 2
MIRT606713 0610030E20Rik RIKEN cDNA 0610030E20 gene 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-743b Propranolol approved 4946 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-743b-3p (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 down-regulated

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