miRTarBase - #MIRT594207


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pre-miRNA Information
pre-miRNA mmu-mir-325   
Genomic Coordinates chrX: 105379082 - 105379179
Synonyms Mirn325, mmu-mir-325, Mir325
Description Mus musculus miR-325 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-325-3p
Sequence 54| UUUAUUGAGCACCUCCUAUCAA |75
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Wdr12   
Synonyms 4933402C23Rik, Ytm1, Ytm1p
Description WD repeat domain 12
Transcript NM_021312   
Expression
Putative miRNA Targets on Wdr12
3'UTR of Wdr12
(miRNA target sites are highlighted)		 
>Wdr12|NM_021312|3'UTR
   1 AAATGAATGGCTGAGAAGAGACTTCCTTGTTGGGTCTTGTCTTAATTCTTAGTGTCTCACTGTTATTTCATAAACTGAAT
  81 CAAATTTGAGAGAGGACTTATAGTACAGCTCAGCTGGTAGAATGCTTGCCTAAAATGTGCACAGCCCTGCATGCTATCTC
 161 CAGCACTGCCCAAAGAAAAATGGGCATGGTAGCACATTCCTGTAATCCCAGTCCTAGAGGTTGGAAGCAAGAGCATCAGA
 241 AATTCAAGGTCACCCTCTGCTGTATAGTGAGCTTAAAGGCAGCCAGCCATGGTTCCCTGAGTCTCTGTCTCCATAAGAAA
 321 GAAATCAATGGATAATCCTCTGATATCATTGTCAATGCAGAGACAGGAAGCAGCCTTTTAAAATTTGGATAACAGTTCAT
 401 CTTTCATAATAGCAACATTTTACTGTCTTTGTCCTTCGTGCTTATAAAATACAGTAAGTATGTGGTCTGAATTCTGTCTA
 481 CATTTTACCCTATGTAGACTCCTAAAACTTGAATTTCAGAAAGTATACTTTTTAAAGGGATATAGTACAAATGTAGTAAA
 561 TTTGTTTTTTTTTGTCTTTTTGTTTTGTTTTAGTTTGACTTTTTAGTTAGCTATTTGAAGAGAAACATCTAGAAAAGACT
 641 AATAATGCTATAGGAGCCAGGCATCATAGCACACACTTATAACCCTAGCATTTAAGAAGTAGAAGCAAAAGGCTTGGGAC
 721 TTGAGTAGGATTGGCTTCATAACTAGTTCAAGTCAGGAGCAGGATGAGACTCGGTCTCAAATAAAGAAAATGAAAACTTA
 801 TAGGAGGGTCATCAAAATGGCTCAGTGACTAAAGATGCTTGCTGCCAATTCTGATAGCGTGAGTTCAGTTCTCAGGTCTG
 881 AACTCCTGCAAGCTGTCCTTTTCCCCACACACATGCACACATACTGTGGCATGCATACAACTAACCTGTAAATAAATTTA
 961 AAGAAAAAAGAAGTTTATAAAAGTATGGGGAAAACAGTCATCTAACATTAAAAGCAAATAGTAAAAAACAACAACAACAA
1041 AACACCACACACTAAAAGGTAGTGTGTATAAGAAAATAAGACTGTTGAGTGTTCAGAGCATTAAAACAATGTAGATGTTA
1121 ATTTGTCCATTCATTGAGCAGCAGGGCAGTGATGGTTCACATCTTTAATGCCAGCACCTGGGAAGCAGGTGGATGTCTGT
1201 GGGTTCAAGGATAGCCTGGTGAGTTTCAGGACAGCCAGGGATAAACAGAGAGACCCTGACTCAAACGCAATAAAAAAAAA
1281 AAATAGTCTTTCCTGAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aaCUAUCCU---CCACGAG-UUAUUu 5'
            |||: ||   ||| ||| ||||| 
Target 5' agGATGAGACTCGGT-CTCAAATAAa 3'
 761 - 785 131.00 -10.70
2 
miRNA  3' aacUAUCCUCC----------A-CGAGUUAUUu 5'
             ||||||||          | |||||:|:| 
Target 5' cttATAGGAGGGTCATCAAAATGGCTCAGTGAc 3'
 797 - 829 129.00 -19.90
3 
miRNA  3' aaCU-AUCCUCCACGAGUUAUUu 5'
            || |||||   |||: |||| 
Target 5' ttGAGTAGGATTGGCTTCATAAc 3'
 721 - 743 120.00 -10.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0004640
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013581. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013588. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013594. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013595. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
27 mmu-miR-325-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT054600 Arc activity regulated cytoskeletal-associated protein 3 1
MIRT577829 Rinl Ras and Rab interactor-like 1 1
MIRT577905 Prr11 proline rich 11 1 1
MIRT578380 Krtap6-1 keratin associated protein 6-1 1 1
MIRT578550 Hsd17b1 hydroxysteroid (17-beta) dehydrogenase 1 1 1
MIRT578770 Gemin8 gem nuclear organelle associated protein 8 1 1
MIRT580288 Trhr thyrotropin releasing hormone receptor 1 1
MIRT581518 Ptprd protein tyrosine phosphatase, receptor type, D 1 1
MIRT582779 Kif1c kinesin family member 1C 1 1
MIRT585752 Stard6 StAR-related lipid transfer (START) domain containing 6 1 1
MIRT587310 Ephx3 epoxide hydrolase 3 1 4
MIRT588735 Taok3 TAO kinase 3 1 1
MIRT592550 Mup7 major urinary protein 7 1 1
MIRT592592 Mup13 major urinary protein 13 1 1
MIRT594207 Wdr12 WD repeat domain 12 1 1
MIRT594462 Epyc epiphycan 1 1
MIRT594693 Atp11b ATPase, class VI, type 11B 1 1
MIRT594929 Gjb2 gap junction protein, beta 2 1 1
MIRT594934 Frem3 Fras1 related extracellular matrix protein 3 1 1
MIRT595784 Hlf hepatic leukemia factor 1 1
MIRT595846 Set SET nuclear oncogene 1 1
MIRT595897 Cd69 CD69 antigen 1 1
MIRT603010 Klk8 kallikrein related-peptidase 8 1 1
MIRT603579 Ppm1k protein phosphatase 1K (PP2C domain containing) 1 1
MIRT604052 Esf1 ESF1 nucleolar pre-rRNA processing protein homolog 1 1
MIRT736870 GHRHR growth hormone releasing hormone receptor 2 0
MIRT756335 Gsdmd gasdermin D 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-325 Mistletoe lectin-I NULL NULL Microarray colorectal cancer cells CLY cells 20955366 2011 down-regulated
miR-325 Mistletoe lectin-I NULL NULL Microarray colorectal cancer cells HT-29 cells 20955366 2011 down-regulated
miR-325 Cisplatin approved 84093 Microarray CNE cells 22614822 2012 up-regulated
miR-325 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) NULL 16078949 Microarray hepatocytes 23107762 2013 up-regulated
miR-325-3p Urocortin 2 NULL 56843276 Quantitative real-time PCR anterior pituitary cells 22252941 2012 up-regulated

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