miRTarBase - #MIRT594345


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pre-miRNA Information
pre-miRNA mmu-mir-1912   
Genomic Coordinates chrX: 147009441 - 147009527
Description Mus musculus miR-1912 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-1912-3p
Sequence 53| CACAGAACAUGCAGUGAGAACU |74
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Mfsd11   
Synonyms 2600014M03Rik
Description major facilitator superfamily domain containing 11
Transcript NM_178620   
Expression
Putative miRNA Targets on Mfsd11
3'UTR of Mfsd11
(miRNA target sites are highlighted)		 
>Mfsd11|NM_178620|3'UTR
   1 TGAGAAAACAAGTGTCTCCAAGAAACACAGCTGGTCACATGGCAGCTGGTTAAATCCGACGGCCTGTCAGTCTCATAATC
  81 GCTAAAGCGAGAACGGAGACTGTGAAATCGGGCGGGGTTGGTAGTAAAGTTTACATATCCTAGCGGCACACGCTAGCGGA
 161 GGAAGGGAATCCCGCTCTATCCGTTACAGCTGTTCTGAGATGTTCGTCCACCCCCTCCCTCAGTTCCTCACAATCAGGGC
 241 TCCTCCATCCTCATTCTCGAGGAACGATCCAGCCTGGCTCGCCATGTGTCACGAGATCCTTGCTGGCTGTGGAGTATGCC
 321 TCACTCTTTCGAAATAGGAAGCAGATGCCACTCCATGTGTGGGTCTGGGAATGACTTTCTGAAACACAGCAAGGATGCTG
 401 AGACACGTTGTTTTGAGACAGGATCTCACTGCGTAGCCCAGGCTGGTCTTGGACTTGCAGCCCTCCCCTGCCTCCAGTGT
 481 TGGGGTTATTGCCCAACACTGGATTTAAAACGCTCCAATCCTAAGAGTTAGGGTCTTGATATGCAGCCTTGGCTGGCCTG
 561 GACTTTGTATACCAGGCAATGCTCCTGCCTTTGTCTCCAGAATGCCTGGGTGGTGAGAATATGCTACCACACACCGCTGA
 641 GACTTCAACTTCTCCTAAAATGCTTTGTTGAACAAGTCTTACCTTGTCACTCCCAGCAAGGGGCTGGTTATGTGTAATGA
 721 GGTAAAGACTGTGTCATTTGTCAACTAATAAAAGCATATTTAGAGAAGGAAAGAGTATTTACTGGGAGGGGGAGGACTCA
 801 TGCTGTGGTTGTCCTGACCGTTACAATAAAGAAAACTCCGGCCATCCTCTGTGGGAAAGTGCGTAAGCACTGCAGACTGT
 881 CTCCCACAAGGACTGACACTCTTACGATTGAGATATTAAATTAATTAAGCATCTAGATCAGGTCTAACATGTATGTACTG
 961 CAGCTTGGCCTCCAACTTGTGCTCTTCCAAGCCACAAACAACCTCTGGCTAAAATTCACCAACTTAGTGTGTATGTGTGG
1041 TGTGTGTGCATAAGGTTGTATAACTGCCACCATATCTAATGACAATATTTTATTTTTAAATTCTTTTTTATTTTATTTAT
1121 TAGTGTATGTGTTTGCCTGTGGCTTGTCTGTATATACATTCTGCCTACAGAACTCAGAGAAGTCAGATGGAGATTGGATC
1201 CCTGCGAACTGCATTTACAGGCAGTTAAAGACCACTGATATGGGGTGCGTTCTCTGGTGTCTTAGTTACTTTCCTCTTGT
1281 GAAGAGATACCAAGGCAACTTGTTTCGAGACAGGGTTTCTCTGTGTAGCACTGGCTGTCCTTGAACTCACGAGGCTGGAG
1361 AATCTGGTTGAGTGCTGGGATTAAAGTGTCCACTATTTCTTTGTTTTGTGTCGATGTACCTTGACGGATGTATTCGCCTA
1441 GCTTTCTTGTTTTTCTTCTGTATTAAAAGTCTGGTGCCTGCTTGGAAAAATTACATTAAGATACACAGTCAAGGGCTGGC
1521 GAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTC
1601 ACAACCATCCCCAACAAGATCTGACTCCCTCTTCTAGAGTGTCTGAAGACAGCTACAGTGTACTTACATATAATCAATAA
1681 ATAAATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ucaagAGUGACGUACAAGACac 5'
               |:|| || |||||||  
Target 5' atccgTTACAGC-TGTTCTGag 3'
 179 - 199 140.00 -12.10
2 
miRNA  3' ucaaGAGUGACGUACAAGACAc 5'
              ||  :| : ||||:||| 
Target 5' tccaCTATTTCTTTGTTTTGTg 3'
 1389 - 1410 134.00 -12.20
3 
miRNA  3' ucAAGAGUGACGUACAAGACAc 5'
            ||||:::| : | |||||| 
Target 5' ctTTCTTGTT-TTTCTTCTGTa 3'
 1442 - 1462 131.00 -7.50
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0014958
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013581. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013594. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013595. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
43 mmu-miR-1912-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577511 Tmem100 transmembrane protein 100 1 1
MIRT577755 Shh sonic hedgehog 1 1
MIRT578803 Foxl1 forkhead box L1 1 1
MIRT579268 Btnl9 butyrophilin-like 9 1 1
MIRT579274 Btn1a1 butyrophilin, subfamily 1, member A1 1 3
MIRT579457 Acsm2 acyl-CoA synthetase medium-chain family member 2 1 1
MIRT581022 Sirpa signal-regulatory protein alpha 1 1
MIRT581556 Ptchd1 patched domain containing 1 1 2
MIRT581946 Pex12 peroxisomal biogenesis factor 12 1 1
MIRT582622 Lrat lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase) 1 1
MIRT583140 Vimp selenoprotein S 1 1
MIRT583710 Ezr ezrin 1 1
MIRT584224 Col24a1 collagen, type XXIV, alpha 1 1 1
MIRT584232 Cntn3 contactin 3 1 1
MIRT584483 Caprin1 cell cycle associated protein 1 1 1
MIRT586095 Reep5 receptor accessory protein 5 1 1
MIRT586114 Rdh1 retinol dehydrogenase 1 (all trans) 1 1
MIRT586222 Pter phosphotriesterase related 1 1
MIRT586885 Htr1a 5-hydroxytryptamine (serotonin) receptor 1A 1 1
MIRT587345 Efcab4a calcium release activated channel regulator 2B 1 2
MIRT587504 D3Ertd254e DNA segment, Chr 3, ERATO Doi 254, expressed 1 1
MIRT587620 Cmklr1 chemokine-like receptor 1 1 4
MIRT588930 Sgms1 sphingomyelin synthase 1 1 1
MIRT589357 Osbpl8 oxysterol binding protein-like 8 1 2
MIRT589494 Mzt1 mitotic spindle organizing protein 1 1 2
MIRT589912 Gng2 guanine nucleotide binding protein (G protein), gamma 2 1 2
MIRT590951 Stxbp4 syntaxin binding protein 4 1 1
MIRT592096 Slc25a42 solute carrier family 25, member 42 1 1
MIRT594345 Mfsd11 major facilitator superfamily domain containing 11 1 1
MIRT595009 Creb5 cAMP responsive element binding protein 5 1 1
MIRT596577 Zfp790 zinc finger protein 790 1 1
MIRT596663 Zfp322a zinc finger protein 322A 1 1
MIRT597521 Rbak RB-associated KRAB zinc finger 1 1
MIRT598049 Nip7 NIP7, nucleolar pre-rRNA processing protein 1 1
MIRT599553 C3ar1 complement component 3a receptor 1 1 1
MIRT600483 Rasal2 RAS protein activator like 2 1 1
MIRT601104 Btrc beta-transducin repeat containing protein 1 1
MIRT601424 Tulp3 tubby-like protein 3 1 1
MIRT601924 Onecut3 one cut domain, family member 3 1 1
MIRT601995 Mrpl15 mitochondrial ribosomal protein L15 1 1
MIRT603982 Arl14epl ADP-ribosylation factor-like 14 effector protein-like 1 1
MIRT604409 Ado 2-aminoethanethiol (cysteamine) dioxygenase 1 1
MIRT606424 Fam96a family with sequence similarity 96, member A 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-1912 5-Fluorouracil approved 3385 Microarray CNE cells 22614822 2012 up-regulated

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