miRTarBase - #MIRT594490


—
pre-miRNA Information
pre-miRNA mmu-mir-470   
Genomic Coordinates chrX: 66813951 - 66814025
Synonyms Mirn470, mmu-mir-470, Mir470
Description Mus musculus miR-470 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-470-5p
Sequence 9| UUCUUGGACUGGCACUGGUGAGU |31
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Dcp2   
Synonyms 2410015D23Rik, 5730537H01Rik, AL118268
Description decapping mRNA 2
Transcript NM_027490   
Expression
Putative miRNA Targets on Dcp2
3'UTR of Dcp2
(miRNA target sites are highlighted)		 
>Dcp2|NM_027490|3'UTR
   1 GCCAGCAAGAGTATTGCAGAAGTCCCTCGATCTGAGACCTGTTGCGTGTGAGTGGCTGTCTCCTCAAGCCTTACCTTTCT
  81 CAGGTATCTGAAAGAAGTGCAGGGAGGCCATGTTTCTAAAGAGATGTTCTGTACAGACGAGAAACTAGAAAGAAACACAA
 161 GTTTGCACTGTAAATGCATTTAGAACTTTTTATATGGATTTACCATTTCAGTGTTTGGAACCAAGTTTTAGTTGCCTGTG
 241 TTTTTTTTTGTCTCTCCCTTATTTTTGTGTGACTGTGTTTTGTTTTATATTCTCATCAAGAGTTGTTTTGATTGCATCAT
 321 CAGCAAGTAGCCATGTCAATAGGAGTGTTTCACCTCCCCATCTTAGTGATTGCAAAAAGCTTGCTTATTGAAAACTTAAA
 401 GGAACTTAAAACCCCAGTTACCAATGTACTGGGCATCAGAGCTGAACCGTGAGGTGAGGATGCAGGCGTTTACTCATCTC
 481 TGCCTCTAACCACTGAGAGTCTAACCTTCCAACTGGAGCTGCACAGTGAACCTCGAGCACTTCATCAGGACCACATCCAC
 561 AGCCTGGAGAGAGATGGAGTCAGTGGGGAGCTGATGCTGTGGATATTGAGCTCTGATGTTTGCTGTCAAAAACCTGTGAG
 641 TGCTGGAGCAGAGAGCATGGAAGCAATAGCTGACAGTTACTCCAGGTCATGCCTTGACTCTCCTGCCTCCGTCCGTTCAG
 721 TATTGTGTCGTGATTATGCCTGTGTGAAACGTCTATGCGTATTCTAGTTGTGAGTCTGTGTGGAAAACTTCATTCTTAGT
 801 TCTATATTGCATCTCAACGTTGATCTGTGTAAGCTGGTATTATGTTAGGTTCCAGTTTGCAGTAATACTAGACTGACATG
 881 CTTTTCATTAATATCTTGAAGCCCCTGAGTTACAACGTGAGAAAGAAGGGGATCTGCAGTTTACCAGTAAGTCCAGCATG
 961 TCACTAGGGGACCGACCCTTGAAGTAATGTACTTAGATCACACGTAGGAATGTATTTTGTATTTTGCTCCTTCAGAATTC
1041 TTAAACCAAACACGGGTGACAGTGCCCACTTTGCAGAGATCGCCTGTTGTTGAGGACAATCACCAGAGCTTTAAGGAGGC
1121 AATATGATGTTGGCTGCCCCTGACCTCGCTGAGAATCTCTTCTGGGGTGGACTGTGCTGTTAGAGTGCATGTGTACACGT
1201 GCGTCTGTCGTGTGCGGTTTTAACAAGTGGTATCCACAAAGTATATCTCTGCTTATAAATAGTACTATAGAAATCTCTAT
1281 GCTGTATATGTAGCCTATTTAACTGGGGGTTCTGCCTAACCCCATGGGACTTAAAAGGAAGGGCTATTATTGTAAGGAAT
1361 TCACTTGGTATTACTACTTTGCTTTAGCTTTTAAATGACTGATTTTAAAGAAGAAATTATATTTATTCATGAAAGTGTCA
1441 CTCGGTGCTAAGCAGGAGACTTCACAGTGATACAGTATGTTTGGAATCTTCTATGCGACTACTTTGTGAATCCTTCTGTG
1521 ACCTTTGCTTTTTAATGCATTATATGATTGTGTTTGGAAGCCCTTACTGTTCTTGTCAGAAATACAGCTGCTTTGCTTGG
1601 GTCTTCTTGAGCCATATTTATAATTGGTCCCAGCATTCCAGATTTCTGTTAAATGGTTAAGGGAGTGAAAACTGAAGCTT
1681 GCCTGGCAGTGAGCCACTGTCACTCCCTGACTGTCACCAAGAAACCATTGTGTGTGTGTGTGTGTGAGAGAGAGAGAGAG
1761 AGAGAGAGAGAGAGAGAGAGAGAGAGATATTCTTCTTAAGTGAGCACTTCAGTCAGTCCCTGGTGCCTGTAGAGGTTGGA
1841 TTCTCAGCTCTCCATACTAGAGCAAAATCAACTAGCTAGCACAAGGTCCATGGCCCTTAAAGTGGTTGACTGTAAGAACC
1921 AACCAGTGATGTGCTGCTTCATTTAATGCACTGTGGAACAAAGCAAGGGCATGGTTGGGGTGCATTTACAATAACTTTAG
2001 GCTTTAGGTCTCTCAACAGGCTGAAATGTTGAGAGAGTAAGGTTTGTATGTGTGATGTGACTCAACTATGCAGAAAATGT
2081 TAAGTTGGTGTTCTTGTTAATGTAAGTCCAGCGTACACACTTCTTCACTGTACAGTTTAGCTGGGAGACCATATTTAGCA
2161 CAGGACGGGATTTCTTCTCTGAGCTATGTTAGCGTTTCTTCATAGGATCTAGTGATGAGGACTCTGAATATGCAGTAATG
2241 TGCTTCTGTGTAGTGCCGCATTTAGAGACAGACTCTAGAGGGAACAGTAGATACGTCGGCCTTATTATTATTTGTGTTTG
2321 AGAGGTGAGACTTTAAAATCGCTTTTAAACATCCTTAGGATCCAACTGCATGTGTTCCGAGGTGCAGTGCACTGTGAATC
2401 TTTTTAAGTCTAAGTTTGTAGGTGCTTTTATGTGTAACTTAAGTGGTTGGAAGAATGTTTAATAACTTTGTCCTCAACCA
2481 TTGTACTCAAAGAGACAAAGGATACAGTTTACATACTTTCTGTAAGATTTTGTTAAGAAATAGTGAATGAATGACTCTAG
2561 GATTTCAAACAGAGATTCCATCCTGAGACATGAGCACTTGGAGGGGCTGGAAAACCCAATAGATCGGATAGCTTGGAAAA
2641 CTTGGTGAGCCATGTTGAAAGAGCTAACTAGATTTCTGTACCAATATGTGAAAGATAGTTGCCCAGAACTGTCAGAGGGA
2721 TTGTGGTCACTGCTTGCTGCTTTGACCTTTGAATATCATTATTTTTATATTAAATACTAATTAGTAATTAGCTATATAGC
2801 AGATTAATATTATGCTGTAAGACTTGGCTAGGCTTTTTTTGGGAGGCTTTAATAATTTACATGTAATGTCAATAAAAATA
2881 CAAATCTAAGAGAATGGCCACAGGAATCAGATTCCTTTATAAAATACTGTGTTTTCTAAAAATGTCAAGGACTGGCACCT
2961 GTGTGCATTAAAAAACATTTCATTACCAAATTCCTTACCAGTCCTGTTTCTCTAGCATTAGTTCAACAGTAAGGAGAGAG
3041 TGGGACCTCGAAATGATAACTGGCTGGACAGGTTTTATTCCCATAATGCCTCTTGGCCTTTGCTGTGTCTATGCGTGCCA
3121 TGTTGGAGATTTTAATGACAGCTGTTGTGTCCATGTTCTCTGAGGCAGTATTTTCTTTGTATTATTATTCATGGTTGTAT
3201 TCATTAATACTTCTTCTGTTTTTGAGTTGGGTTATTGTGTATTATTATTATTGAGAAAAGAGGTTCAAACGTGTTATAAC
3281 TAGAATTGACCATCAGCAAAGGACACCACTCCCAGGTCTAATGGTATTCTTCACCTTTGTGTATGTAGTGTTACTCCTTA
3361 GAGCTCAGTACAGCTGGTGTCCTAACACTTCAGAATACATTTGTATAGTTTCTTAAAAGCCTAGAAATGGGCTTATAGAA
3441 GGCCTCTCCACACCTTCCTATCAATATATGTAGGATTGTCACTCCTGAAGCCTAGGTCAGAAGGCAGGTTGAGATCAGCA
3521 GAATGAGATTTGTCATAAGGGTTTTGCTGTTTTAAGTGTGTTGTGGGAAAGGATCTTGAAAAGAACAGATAGCAGTTGCT
3601 GGGAGGAGTGAGGAAGAAACTACTACGCATTTGGCACTTGAGTGCTTCCTGGAAGGATGCTCAGGGGAGAAATGTGGTCA
3681 CCCCCTGCCCAAGCAGAATTGAGCATGCAGTTCTTTCCAGGCATTTTCTATTCCTTGGTGAAGTATTGTTTTCTAAGTAT
3761 GATCGCCAAGCAGTCCATGGTTGCTAATGGAATTTTTTTCGCTCTAAGGACCAGTGTTCGAGTCCTTTCTGTGTTGATTC
3841 TGTCAAGCTCTAGGCTGAGTTTGGGTTTTGTTTGTTTGTTTGGTTTGTCATTACTTTAATTTGTTTTTAATGTGTATGTA
3921 TGTTTTGCCTGTATATTTGTTTGTATACCACATGTGTGCCTGTGGTGCCCAGGGAGGTCAGAAAAAGTGTGTCGGATAAC
4001 CCGCCCCCCTCCACCCCCCGGAACTGGGGTTCAGAGCAATTGGAAATGGAACCCAGGCCCTCTGCCAGAGCTCTCACCCT
4081 CTCTGTTGTTGATAACAAGGTAGCCCAGAGTGTTGCACAGTCATGTGCAAATGCTGGCAGCACTTTCCTTGTGACAAACA
4161 GCATTTGTCACTGAAGTACTCTACAGCCATTGAATAATGGAGACTCAGCCCACCCTCCTGGGTAGAACTGGATGAAAGGG
4241 CATTTCTGTCAACGTAGTTCATCAGGAAGGTTGTTTGTTTAGGTTTTCCCTGTATACATCTTTGTACTTCGTGTTAAAGC
4321 TCAGAGGCTTCATTTTGAGACATGTTCAGAGTGGAATATCTAATTTGAAGCCATCCATAAAGAGATTACATTAATCTGGA
4401 GTCTTAGTGTAGCACAGGGGTTGGGTCTGTGTCTCTGTCACAGTTTAAAAGGAATTCACCTCAAGATGTGACTAGTTGGG
4481 CAGGATTGACTAAGGGCCCTCTGGGAAAAGGAACCCCAGGAGTATCAGTTTCAGGGTGTGACTTGGTCCTCCTAGTGGAC
4561 ATCAGCTCTTTGGTGGAAGGTAAGTGTGTCAGCTAGGGGGCGGGGGTGGTTTTGTTGATATTGTTACTAGTTTGTTAAGT
4641 GTCCTTTTCTGAGCATCTGTTTCAGTATTTCTCAGGCTTTTTGTTTGTGCTACGTCTCTAATACATCTGACACCATGAGA
4721 ATTTGCTGTAAGTTTAACTCCTGAGCCTCAAGTAGATAAAAGGGCTGTGTTCTCTCAAGTCAGTGTCTATAGCTGTGAAA
4801 TTGGTGTGGGAGCACAGAAAACACTTTGAGGTTCTCGTTGGTAGCTTTAGAGTGGAACGGGCAGCATTTCCTAACACTCA
4881 GTTCTTAGGATAGTGAGCTTTTAAAGTAGAAGGTAGGGTGCTTTCTAATTTTAATTATTTGATCCAAATGTGTAAAACGG
4961 TAGCTCTAGACTGGTGAAGCATTTGGGACACAGTTGGACATTTTAAAAGCCATTTCTCAAGCTTTAAGGCATTTAGCACA
5041 GCCTTTGCAGTGCTCAGCAGAGGCGCGAATGCAAGCAGAGGCGCGGCCATGAGGTCGGTGTCCGACGCTGGCCGTGGCTG
5121 GAGAGATGCAGTAATACTTGTGGAGTGTGAGCGGCAGTGGATAGGACACGTGACGTGCACGGTGCCTTGGGAGAGCATGG
5201 GCTGGTCCTGCAGGACTCTGCATTTCACTGTGACTGTGCAGCACATTTTAGGCTGTGTTTGAATGTCTCACAAGTTACTG
5281 TTAAGTGTCAAATGCAAGAATCCAAGAAGGAGCTGAGCCTTTCATTTTACATGTTGTTTGAAATTAGGACAAGAGACAAT
5361 TCACTTCTGTTGTGTAAATAGTAATTTTTTTTTCAGTTCAGCAAATAGGGCAAAACAGTGAAACCATTAACTTCTAGTTT
5441 TTGAAAACTATTTTTTTATATTTACTGATAAGTTTTAAATCTTATGGTAGTTGTAAAAATGAGTATTGACTTACTAGACT
5521 TATGTATAATTCAAATAATCTGTTGGCTTCAATGAAACCGTAATGAATCTTCATGAATGTTCATGTATTTAGCTATTTTA
5601 AGTGATATTCTCTTAATTTGGATAGTCTTAGGTCAGGCCAGTAGAGATGATGCCCACCTGTAATCCCAGCACTTGGGAGG
5681 CAGAGGCAGGAGAGTCTCTGAGTTTGAGGCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCAGGGCTACATAGAGAA
5761 ACCCTGTCTGGAGGCAGGGGTTGGGGGTGGGGTTGGGGGGGTGTTGTGGGTGGAAAATGGGCGGGGGTAAGTTTAACAAA
5841 ATTGAAGCATAGTTTGGGTGCAAAAGTGAATAAAATTTGGATATTTTTATGCACTGTGTCTTTGGTTTGTAGTAGTTTAC
5921 ACTCTGCTGTAAAGACCTGATGTTCCTAAACAGTGTAGTTTTCAAACAGTTTAATTCTAGGTATGTCTGGGAAAGTTGTA
6001 AACATGCAGAGTCATAGAAGCTACAAAAAGCTGGTACATGAATTTTGGTGAAATTGCTTCATTAATGATACAATATTCAG
6081 TGAAAAACCTAGGCTTCAGAATAAGTAGATTTGTAAACTGTTTCTTCATTAATTGGTTTTGTCTTATATAAAATGATTTA
6161 ACATAGAATCTCTGCCAGACTTATAACGACGCTTTATATTTTATTGGGAGAAAGATGTAAGCAAATCCTTTACATGATTA
6241 TGTCTATGATAATAGGAAAGTATTTGCTTTACTGTAGTTATTAGTCAAACATTTAGGGAAACCAAAGAGGAACCCCCAAA
6321 ATAGCTCGTTGGGTTTACTGTAGTGTGACAACATCCTTTAGTGCTACATTTATTTAGTGAGCCCCTCCCCCAAGTTTGGT
6401 GTTTATGAATAATTGGAACTGCTTTTCACCTACATCGATGTAACTCATAAATTTCTAGTGACTCGGGTCTGTGAATTAGT
6481 GATGCTGGAGCACCCACCGCCCTTCCAAAGATGTGGGGTGACCCGACACCTGTGCTGTAACATCTAACAGCTTTTCAGTG
6561 TGATGCACACTAGTTTAGGAGCTAAAACCATACTGTAAAGAATGCACTGACAGTGATGGAAGGCCACCCACGCTGGCTTT
6641 CTGTCGAATACTGTGAGCTTTGCTCTAACTCTGTCTGTAAGGAATTACCCATAAACTGGGCTGCTTGGGCACAGTTACTG
6721 CTCTGCCTTTGGTTCTGTTCTGTTTAAAGTCATTGCTGTTACTGGTTATTACTCTGGACCAGAAAGGCTAGGAATGTATA
6801 GGATAAACATGAGAAGTTGTTCGCATTCATAGAAATATAAAAGGTAGAATTTATTCAGAGATGAAAGTGGCCTGTGGGAA
6881 TGTCACCTTTATCTCAGGTCGGCTTGGCTCTTAAGCTGCAGTACAGTGGTGGGCAGGAGATAAAAATCCGACCTGTAACG
6961 TGCAGTGGTTCTTAGGCTTGAGCCAGAACCAGGCAGAATGTCCAGCAGAGTGTCCTATTGCCCCTCCCTCTCAGGCAGGA
7041 GAGTCCTCAGCTACGTAACTATGTTGTCATCTTTCCATGGAGCAGAAAGTCTGTGTTTCGACACTCCTGATGTGCTATGA
7121 AACTGCTTGCTATCTCCTAGGTTTAGACTTCTTGAAACTTTTTTTCCTGTTTTCAGTAGAGAAACTTCTGTTTATGCTGT
7201 ATGTGAAGACAACCAAATAAAAATTGATGATAAATGAGAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugAGUGGUCACGGUC--AGGUUCUu 5'
            |||  | ||| ||  ||||||| 
Target 5' tgTCA--AATGCAAGAATCCAAGAa 3'
 5286 - 5308 159.00 -12.90
2 
miRNA  3' ugAGUGGUCACGGUC-AGGUUCUu 5'
            ||  ||| |: || ||||:|| 
Target 5' ggTCTACAGAGTGAGTTCCAGGAc 3'
 5717 - 5740 145.00 -15.30
3 
miRNA  3' ugAGUGG---UCACGGUCAGGUUCUu 5'
            ||| :   | | | | ||:|||| 
Target 5' tgTCAATAAAAATACAAATCTAAGAg 3'
 2867 - 2892 133.00 -7.90
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0002111
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013575. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013576. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013579. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013580. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013594. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013595. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
26 mmu-miR-470-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001002 Nanog Nanog homeobox 4 1
MIRT004344 Pou5f1 POU domain, class 5, transcription factor 1 4 1
MIRT005701 Ass1 argininosuccinate synthetase 1 2 1
MIRT579600 4930563E22Rik RIKEN cDNA 4930563E22 gene 1 3
MIRT579658 1810030O07Rik RIKEN cDNA 1810030O07 gene 1 2
MIRT587091 Gldn gliomedin 1 1
MIRT591592 Zbtb44 zinc finger and BTB domain containing 44 1 1
MIRT594490 Dcp2 decapping mRNA 2 1 1
MIRT596386 Wipf2 WAS/WASL interacting protein family, member 2 1 1
MIRT596756 Utp23 UTP23 small subunit processome component 1 1
MIRT597051 Terf2ip telomeric repeat binding factor 2, interacting protein 1 1
MIRT598179 Mrpl19 mitochondrial ribosomal protein L19 1 1
MIRT598583 Intu inturned planar cell polarity protein 1 1
MIRT599191 Ddx19b DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b 1 1
MIRT599360 Colec12 collectin sub-family member 12 1 1
MIRT599386 Cln8 ceroid-lipofuscinosis, neuronal 8 1 1
MIRT599752 Amy2a4 amylase 2a4 1 1
MIRT599756 Amy2a3 amylase 2a3 1 1
MIRT599760 Amy2a2 amylase 2a2 1 1
MIRT599869 Acnat1 acyl-coenzyme A amino acid N-acyltransferase 1 1 1
MIRT600012 2900026A02Rik RIKEN cDNA 2900026A02 gene 1 1
MIRT601650 Siglece sialic acid binding Ig-like lectin E 1 1
MIRT602197 Hmmr hyaluronan mediated motility receptor (RHAMM) 1 1
MIRT605471 Slc7a14 solute carrier family 7 (cationic amino acid transporter, y+ system), member 14 1 1
MIRT605570 Oxsm 3-oxoacyl-ACP synthase, mitochondrial 1 1
MIRT732694 BDNF brain derived neurotrophic factor 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-470 (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 up-regulated
miR-470 (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 up-regulated
miR-470 (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 up-regulated
miR-470 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 up-regulated

Error report submission