miRTarBase - #MIRT594593


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pre-miRNA Information
pre-miRNA mmu-mir-465c-1   
Genomic Coordinates chrX: 66825955 - 66826035
Description Mus musculus miR-465c-1 stem-loop
Comment None
RNA Secondary Structure
pre-miRNA mmu-mir-465c-2   
Genomic Coordinates chrX: 66832517 - 66832597
Description Mus musculus miR-465c-2 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-465c-5p
Sequence 11| UAUUUAGAAUGGCGCUGAUCUG |32
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Tfap2b   
Synonyms AI606113, AP-2(beta), E130018K07Rik, Tcfap2b
Description transcription factor AP-2 beta
Transcript NM_001025305   
Other Transcripts NM_009334   
Expression
Putative miRNA Targets on Tfap2b
3'UTR of Tfap2b
(miRNA target sites are highlighted)		 
>Tfap2b|NM_001025305|3'UTR
   1 AAAAAAAAAAATTAAAGAGAAGGAAAATGTTTTAAATACAAAAGGAAAACAGAAAAAAAATAAATTTAGCTTTAACATAT
  81 TGGATTGGCTTTGGAGGAATTATATTAGGTAGAATACACATACAATCAAAATTTAAAAAGAAAGCTAAATAACGTTAAAA
 161 AAAAATAAACTGAGGCATACAACGGAGCAACAAACCGGCTCTCGGTGTCTATTTCAAGATACACGTGGAGACAACCGTCC
 241 GGATTTTCCACTTCGGTTCTTTCGAATTTAGTAATACAGATAAAAAAGGAAAACTATGAATCCCCCTTTTGGAAAATAAA
 321 ACGTGAGACTAAACATGAGAACACGCTAACTTATTGGAAGAAAATTGGAGAAACGTTGGTGTCAATGCTTTTGAGAGCTG
 401 GTTGACTGAGACGCACGAACTTTTAAATCTTTAATTTAATTTTTTTTTTTTTTAAGGAAACTCTCGCACTTCGCGCCCTC
 481 CATTTCTCCTCATTCTCCTCACCGCTGCTTCCCTTTCTCCCTTCCTCACAGCGATCCCGGAAATCTGCTGGGTGATCACG
 561 AGTGTGATTAGCTCTCTGGTGTTAGCCCCTTTGGTGTCCAGACCGCGCCTTGCGTTCCTAACTCCCCTGAGCCCAACTCC
 641 CCTACCTCCCCTCCACCCCCACCCTCACCCTCACCCCGCCCCGGTGTGTTTAATTAGGGAGGCCAAGGGTGGGGGTGGGG
 721 GACAGGCGGAGGCTCTAGCTTTTGATCCAGGCTTGAGAGCCCAGGCTCCCGCGGCAGAGAGGAAACCGATAAGTTGATTC
 801 ATCTTGGTTCCTTTTAGAAAATGTTACTAGTCTCCCAGACGTGACAGCACCTGTTCACAGAGAATATTACATTTGTTATA
 881 CTTGTGTCATCTAGCGGTATAGTTTAAAAAGAAAGGAAAAGAAAGGAAAAAACAAAAACCAAAACATGAAAACTTTGATT
 961 AGTATGTAATTCCTTCGGAGGGGTATGTACCACTGACTCTCTTCTGTTTCCCCAAGCTTTACCCGCATGGTTTATGAGCT
1041 TCTTCAAGGAAGATTTGGGCTTCCTACACAATCTATAAGGTACTGAGAAACAACAGCAACAACCTGATCCTTTTTAAAGT
1121 TAAAGCCTGCCTGTCAGATTCGGCAGGTTTTACTTCTTTAAAGAAGCTCAAAGAGAATCATTTAGGAAGTGGCCAGTGTT
1201 GGGGGGAGCGGCTTTGGATTTCTTGCTAACACACCTCAACGCAGGAATTCCAGCAGGGGCGGGACACTGCAGAATGCATT
1281 TTAAGGGAGTCGAGTAAGGTATCTTGGCTTTGGGACCTATATTGTAATGGTATTGTCTTTACTGGAAAAATGGATTCCAT
1361 TTCCTTGGGCTCTTTGGGGACTGGATGTTTTAATGGGGGAAAGAAGTAGTTTTGCTGAGTGATTGGGGAAGACAGTCTAT
1441 AAACTACCATGACTCTGCTTTATGAATGAAACAGTTTTCCTTCTCAGACACACAGGTATTTTGGCATAAAATGGTACACA
1521 CACACACACACACACACACACACACACACACACACACACCAGAAACACAAGGCAAAGGGAACGAGATTGTATTTCAACTT
1601 TGAATTCCTGTCTTGATTTATTGGGAATATTCCTAATGGATGAAGTTCCATGAATTTAATGTATCTCTCTTCTCTTAGTC
1681 CAACTTTTTACTGTACCCCATCTTAATATTACCACGAAGTAAAACCGGCACCTTTAGCAAGTTCAAGGAGTAGAGTATTT
1761 GATTGGTGCACACATTTAGTTTGGGTACACTTATTTTTTTTCACCTACCTAGACGGGAACTTTATAAAATCCATTAGGAT
1841 ATATGTTCAGATGTTTTCTGTGATTTACACAGTCCCTTCCCCCAGTAGTCTGGACTCTCAGTATTCCTATCTTTTTCAAT
1921 CCACTTTGAAGCAAGGGAAGGGGTTGAAGTTTTAGGGACCCTCTGAGGGAATCGCTCAAAGGATGAAGGTTGTAAACAAA
2001 AATTGAGGTCAGGCAACATCTCTGCTCCTATCTCTAGCTGCTTCTGAGCCCCATAGGTTTCCAGGACACAGCCCTAGACG
2081 AGGCTGAAAGGAGTAGAAAGGGCAGGAAAAAATACATGTGTGAACTATAGATTCTCATCTTTCCTCAGTTTAGAAGCCCA
2161 CCCCATTTGTCCAAGAACCTGGACGCTTGCATTAGTTGAATTCTGATTGCACGAAGCCAGCAGAAAACACATTGAAAAAC
2241 ATCGTCTCCTTTCCTTGCAGGGCAACGCGCCCCACGCGTGTGACATCGAGAGACGCGATGGATGCGCCTTGCTCTTACTG
2321 TGCAGGTCCTGGGAACATGTGGGCCACTGAAGCCCAGTGGTGTTGAGGACATAGAATCAGCAGCTGCTGAGGGCGCGAGC
2401 CGTACAGGCCCTTCTTGCTCTCGGCCTCACCCTAAGGGCTAAGGCAGCCGCGAAGGGCCGGTTCCCGAGTAGGTAAAGGG
2481 GCGGCGCCCCACTGGCTGCTGCGGGGGAGGGATGCCAGAGCTCTGTAAAGCCATTTTGCCGCAAGCGGTCACTAGGGGCT
2561 CTACTCTCAGAGACCTTGTAGCTTGGAAAAGTCCATGGAATTTGAAACTTCCATGTGTTCTTTATTGCCAGGAAGTGTGG
2641 ACATTTTTTTTAATTGCCTGAAGTGTGGACATGAAATAAGAACTGTCATTCTTGGGTAGGTAGGCTCCCTTAGATGCCCT
2721 GATGTTCCTGTGAGCATCAGCGAAGGGGCAGCTAGATAGACCTGGCTTGAGGCTTCCCTCCCCCATCTCCGGGGTTGTCT
2801 TCTGTTTCTGTTCTTTTTCTCTCTCTTCACGTCGGACCTTTCGCCCCCATGCTTTCCTTAGCCAAGCCGGCTACATTTAT
2881 CTGATAATTGAGTTATTAAAAGATTTGGTTTCCTTGGATTTATAAATCAATAAACAGTAGGATTAACGCAAGAGTTTGCC
2961 TTTTAAGTCAAGGAAAAACGTTTAAGGCCAGGCCCTTTGAGTTTTGTTTTCAAACCAAACAGGTGAGTTACAGTCCTATA
3041 CTCTCCCCTGCACTCTGTAGATGGCTTATCTTATCTAGGCCCGCTCCCACCACCCCCAAGCCCTACCAAATGCCGGGGGT
3121 CCAAAACCAAATAGCTAAAGACTGAAGCGTAGGCCGGTGACTGCTGGAGTTCCCCCAAGGTCCTTGGAGCCCCTTCTTTT
3201 TGAATCAAGCATCCTCCTACTTTTATTCTGAGTCTCATGGAGTTTGTTCCTAGGTGAGGATTCCCTCTCCAGAGACCTTT
3281 AATTTTCATCCCAACTACGAAGTAACTTTCTTTTCTTCTTTTCACCAACTTATCTTGCCCCCCCCCACTTCCTCACTTCC
3361 CCACTTACCTGCCCAAAACCTACTCCTTCTTCCATAGCTTTCTCCACCCCCATATAAAGAGCTCAACGGGCTTTTGAAGA
3441 TCCGACCTTTGTTGGCCTTGGCCAGGAACTTTCTCTTAAGCAATTATTTCATAACCTTTGAAAACTGGTCGAATGGGAAA
3521 CCCCAACCTTCCAACTTTTCTACACACTTCGTTCATGGTTACAAGACCCCTCCCTATTTAGAGTATTGATAGAAAATTCA
3601 TCGCCATACCTGATATCCTGTACTTGTAATACATTCTGTGGGAAGGAACTGATGACCTCAAACCATCAGACTCTGCCTCA
3681 AGTGCTCAGAGAAACAAGAGCTTCCCTCCTTTGCTGATATACATTAAATTATTGGTAAAAGAAAAAAAAACCAGAAGTAT
3761 TGGATTGTTTTCTTAAAACAAAACAAAACAAAAACCCAAAAAACACAAAAAAACAACAAAAACAACGGGGTGAACACCAT
3841 TTCTTCTTTAATGCCAAAACCGTTTCTCCTTTTGAGTTGGCCTTTAATTCAGTACCATTTGTTTTGACCACCCAGTCAAG
3921 AGGTAACAACTGCCTCTTCTCGAGGGCACTCTGGAGGGTATCTGTGTTAAGTTTGTATATATTTATTTATGCTTAATTTA
4001 ATGGGAATGTGTAAATATGATGAGCAAGTAGTTTGGGATTATTTATCTGTGACTCTATACCTCTGTGAATGGGTGGTTTA
4081 AAAGAAAAAAAGAACCGCTTGACCCTAGCTAGAATCCTATTTGAATTCTTCTGTTCTTTATAGACAAGCTGTTATGGTAT
4161 TGGGTAGAAATTGGTTTATTGTCCAGTGTTAATCTGATTTACATAAATAAAAAAGATTGTTGTGTTTTTGTTGTGTTCTC
4241 CTCTTCAGTTCCTTAAACATAAAGATTGTTCCATTCTACAGACAGAAACAAAGATATAGCAAAAAAGATTTCTTCCTTTT
4321 GCACCCCCGCCACAGACGTACATACAGTGTTTTTGTTACAACAGAAATATAATAAATTTTGCATATCAGAAAAATGAACA
4401 CTTTTATTTCCAGTAGGAGGTGCTTTTTAAAAAACTCTTAAAACTTCAAATGGATACTAGTCCCAATCATATTTGTAATA
4481 AAAGTTCATGTTAACTGC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' guCU-AGUC-GCG-GU---AAGAUUUAu 5'
            || | || ||| |:   ||:||||| 
Target 5' ttGACTGAGACGCACGAACTTTTAAATc 3'
 402 - 429 136.00 -6.80
2 
miRNA  3' gucUAG-UCGCGGUA--AGAUUUau 5'
             ||| |||| :||  |:||||  
Target 5' gtcATCTAGCGGTATAGTTTAAAaa 3'
 886 - 910 129.00 -8.70
3 
miRNA  3' guCUAGUCGCG---------GUAAGAUUUAu 5'
            |||:| |||         | ||:|||:| 
Target 5' agGATTAACGCAAGAGTTTGCCTTTTAAGTc 3'
 2939 - 2969 124.00 -9.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0004873
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013595. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
20 mmu-miR-465c-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577524 Tirap toll-interleukin 1 receptor (TIR) domain-containing adaptor protein 2 2
MIRT578756 Gm11559 predicted gene 11559 2 2
MIRT579164 Cdc37 cell division cycle 37 2 2
MIRT579596 4930563E22Rik RIKEN cDNA 4930563E22 gene 2 2
MIRT579614 4930430F08Rik RIKEN cDNA 4930430F08 gene 2 2
MIRT581042 Sim1 single-minded homolog 1 (Drosophila) 2 2
MIRT581082 Setd7 SET domain containing (lysine methyltransferase) 7 2 2
MIRT582172 Nkain3 Na+/K+ transporting ATPase interacting 3 2 4
MIRT582549 Map3k1 mitogen-activated protein kinase kinase kinase 1 2 2
MIRT585014 Gid4 GID complex subunit 4, VID24 homolog 1 2
MIRT585345 Ybey ybeY metallopeptidase 2 2
MIRT585446 Veph1 ventricular zone expressed PH domain-containing 1 2 2
MIRT585687 Tbc1d24 TBC1 domain family, member 24 2 2
MIRT588450 Ybx1 Y box protein 1 2 4
MIRT592140 Ppp1r1c protein phosphatase 1, regulatory (inhibitor) subunit 1C 2 2
MIRT593134 Thrb thyroid hormone receptor beta 2 4
MIRT594593 Tfap2b transcription factor AP-2 beta 1 1
MIRT595796 Dapk1 death associated protein kinase 1 2 2
MIRT595818 Arl4a ADP-ribosylation factor-like 4A 2 2
MIRT598674 Hltf helicase-like transcription factor 2 2

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