>Tmlhe|NM_138758|3'UTR
   1 AATCGAAAGTGCTAGGATTATGAAAACACTTGGCATCCTCCTACCAGAATTTTATATCAACAGAATAATATTGTGTTAAA
  81 ATTTGGTTAATGTTGTCTATTTTCCCATTCTACAAAACAAAGTTTTTCCTTACTTTTCTAGGAGAAGCCTGTTAAAGAAA
 161 GAGGGCTTCTGAGTAGTCTAATATCAGCCATCTAGTAGGTTAGATTTCTCCCCAAGTTATAACATAAGTCTAATCTTTTC
 241 CTAATCTTTTTTATATAATTATAATTACTGTACTTAAGAAATATAATCTCAGCCCGGCAGTGGTGGTGCACGCCTTTAAT
 321 CCTAGCACTTGGGAGGCAGAGGCAGGCAGATTTCTGAATTCAAGGCCAGCCTGGTCTACAAACTGAATTCCAGGACAGCC
 401 AGGGATACACAGAGAAACCTTGTCTCAAAAAACCAAATATATATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT
 481 GTGTGTGTGTGATCTCCATATTAAAAGGAAACCAATGGTATTACATAATTTGTCTTTAGCTTATCAGAATACAAGACATT
 561 CTAGAAATGACAAAATACTGATCTGATAAAGCAACATTTGTAAAAAGTTTTTACCTTTTATAATTCATTTGACTAAGAAT
 641 ATATGCTTACTTATATTTTGAATATATCCTATAATATCTTCTAGCACCTTCCAGTTTCAGAGGTTGATGGGATATTGTTT
 721 TTCTTTTTGATGAATGAATTCCATTGTGCTACTATGGATATATATATATATATATATATATATATATATATATATATATA
 801 CATACTATGGATTACCTACTATGGATAATATATCAATAAACATGGACATGCAGATAAAAAAAAAAAAAAA

ID	Duplex structure	Position	Score	MFE
1	miRNA 3' agAUAAGUCGUACU--ACAGAAAg 5' |||| | ||| | ||||||| Target 5' ggTATT-A-CATAATTTGTCTTTa 3'	517 - 538	156.00	-7.10
2	miRNA 3' agaUAAGUCG--UACUACAGAAAg 5' |||::|: ||| ||||| | Target 5' aaaATTTGGTTAATGTTGTCTATt 3'	78 - 101	133.00	-7.32
3	miRNA 3' agaUAA--GUCGUACUACAGAAAg 5' :|| ::| || |||:||| Target 5' gagGTTGATGGGATATTGTTTTTc 3'	700 - 723	133.00	-8.00

- Molecular cell, 2012

MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.