| miRNA Infomation | |
|---|---|
| miRNA name | mmu-miR-1194 |
| Gene Information | |
|---|---|
| Gene Symbol | Hs6st2 |
| Synonyms | 6OST2 |
| Description | heparan sulfate 6-O-sulfotransferase 2 |
| Transcript | NM_001077202 |
| Other Transcripts | NM_015819 |
| Expression | |
| Putative miRNA Targets on Hs6st2 | |
| 3'UTR of Hs6st2 (miRNA target sites are highlighted) |
>Hs6st2|NM_001077202|3'UTR 1 CTGGCACTCAAAGGCTTGTGCACACTGCTTCCCAAAGCATCACCAAAAAGATGGCGTGTATCTTACAAGATGAAAATGTC 81 CAAACACATCCTGCTTCCTTCATTTGGGAAGTTCAAAAAAAAAAAGTGTAGACGTTGCCTTTACAGTTGCCTTTTGATTC 161 AGTGTTATGCTGTGTGTGGGTACAACAAATCTCAGTGTGTAATTAAATTGTCTGTTTTGGGATGAACTACTTCTGAAATC 241 CAAGAGCCAAACCAGACTCACCAGAAATTGCAGCTTAGATATTTTAAGACGTTCTTAAATTAGGTATGGGAGACACAAAG 321 AAAACATGAAATGTGGCCGTTTAAACTTATGGCTAAGAAACAGACTTTAAGTGATTCCGGATACTCTGTTAAAACTCAGT 401 CTTGAAAGCACCCCCCCTCCCTGCAGGGAAGTATAAGTAAAAACATGAACAGAACTAAAAGACACTCAAAACTGTTCATT 481 TCCTTGTTTTAACAAGGAGCCTATTTAAACAGAAAAACAACTGATGATAAATTTTGCCAAACTGTAGAAAATAAACAATT 561 TCTCACTTAAATGTAGCTGGGTTTATGTAAAAATAATGGCTTTTCAAATAGATCGGGATTCGTGTCTTGTAACTTAACAG 641 ATGTTTAAAATTTAGAATTTTTCTACCTTCTGCTGATTAAAAGGTTTTAAACAGGGTATATCTCATATTAACAAAGCATT 721 TTTTCCAAATGGAATACCAATGGAAAGATCCAGTTTCCAGATGGTTTCTGGGCACTGTAATTCCAACAATTTCGATCTTT 801 TTGGCGCTGCTTCTCATTCACTTTAAGGCTTCTCCGAGTTGTGCTCATTCCAAATGAATCCATGTATAGAATTTTTCTTC 881 TTCATCTAAACAACGTGTTGCTAAATCTCTTGTGCTATTTAATCCTGGACTAAAGTCAGGACTTTCTATAGAATTGTCTT 961 ATCAATGTCTGTGTAGTAAGGTCCTTAAGAAGAACTCTCAAACAGAATAATTTGTCTCTTAATATTGCTGAAGTTATTAT 1041 TCTTCATCAGATTGAACTAAACCTGAATGTGAACTGTTAATTATCTTGTTTATTGTAATGTTTCCAGTTTGGCTGGCTAT 1121 TTCTGGCCCATGGTTCTTTTCTTGTATTGACAGATTTGTACACAATAATAAGAGCAAAAAATTTTCTCAAAGACAACAGT 1201 AGACTAATCATTAAGATAGCCAGAGTTGCTCAATAACACCAAAAGCTGCCTCTTGATAATTGAGGGGAACACTTCTACCA 1281 GGATTTTGTTTGTGAAAGATCAAAAATAAAGAATGTTTTGCAGCGAAGCTAAGTGTCAGGGACGTGCAAGTAGAGGAGAA 1361 TAGTTTGATAGTCTGCATCTGTGAAGAGTATTTTGATGGTTTCAATTACAAATGGAACAAAGGAAGAATTATATACACAC 1441 TGTGTTGTGAAATATCATACTGCTTGGCAGTTTACACCTCATGGTTGTTGCTACACTTCTATAAGAGCTTACATTAATCC 1521 CTTGGCTTTCAATCAAAACAAGGTGACCAGAAGTGGTATTCATGCTTTCAAGAGGGACATGCTTAGAAATATGATCATAA 1601 AGTCACATCTGTCATAGTCTGCCCAAGTGCATGACCTGGTTTCATCTGTAATAATATGGCCACAAGTTTGGCAAGAAGTT 1681 GAGTATCTGGCTCTAAAGAGATTTTCTTGACTCTGAGCTCTGTGACAATATTTGCAGCTTTTGTTTTGTGGTATTTGCAG 1761 TGTTGAGAATGATGATACCTAAAAAAAAATCAAAAAACAAAAACAAAAAAATGAAGAAAAAACAAAAACAAACAAAAAAA 1841 AACTGGAAGGAATTTTTAAAAGCACATCTTGAAGTCAAAGTATTTGGTGTACATTGTGTTCTTGAAAACTTCTGTAGATC 1921 ACTATGGTATTTCAGTAATTTTAGTTTTAATTGAAAATGATTGTTCTTAAAACTGACAGTGTTGGAAAGCAGGACGAAGT 2001 CTGAGCCAGTGGAAAAGCTCATTACAGAAAAAAAAAAGTGTTATTGCTGTGGTGTGCATGGTTTGCAGAGATTAAGTGCA 2081 TTTTCTCTGTCTCTACTGATTATTGTATATAGAGAATGTTATAAATATACATTTTTGTCATCATCATGTAAATCCCACGA 2161 TTTCAAACTGTAAACATCTGTTCAGTGGTGTAGCTTTACAAACTGTTCACTGATTTTGTGTAATTTATCAATAGATGTGA 2241 AATAAAGTTTCAATTGCAGGCTGTGAGTAATCTCATGATTTCAAGGTTTATAGCCAGTAGACATTGTGATACCATAAACA 2321 AACCAGAACAGGAATCGTTTCAAAGACAGTGTTTTTCTTTACACTTGACAGCTAAGTATGAAAATGAGATGGCCCATTTT 2401 AATACTCATGCTGAAATGGCCTTAATTTGGGATGCTTTTTCCTTGGAGGCCATGATTTGAATAAAACAAAATTGTTCCTG 2481 AAATAAGGTTTGCCGTGCAAATGAAGTGAACCCACGTTCTCATTTAAAGTGTATATAGTAGATGCTTTCATATATCATAG 2561 AATGCACAAAGGGAAAAAAATAGCACTCTTAATTACTTACCTCCTTTTATCCACAAGAAAAAGGTTAAAGGTAAGCATTA 2641 ATATTTTGGCCTTGGGGTCTGGAGCAGTTAAGAGCACTGACTGCTCTTCTGGAGGTCCTGAGTTCAATTCCCAGCAACCA 2721 CATGGGAATGAACCCCAGCTCACAGCCATCTGTAATGGGATCTGATGCCCTCTTCTGGTGTGTCGGAAGATACCTACAGT 2801 GTACTCATATAAATAAAATAAATTTGACAAAAC Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
| Experimental Support 1 for Functional miRNA-Target Interaction | |
|---|---|
| miRNA:Target | ---- |
| Validation Method |
|
| Conditions | CD4+ T cells (C57BL/6) |
| Disease | MIMAT0005852 |
| Location of target site | 3'UTR |
| Tools used in this research | TargetScan , miRTarCLIP , Piranha |
| Original Description (Extracted from the article) |
...
"HITS-CLIP data was present in GSM1013594. RNA binding protein: AGO2. Condition:CD4+ T cells
... - Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell. |
| Article |
- Loeb GB; Khan AA; Canner D; Hiatt JB; et al. - Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
|
| CLIP-seq Support 1 for dataset GSM1013594 | |
|---|---|
| Method / RBP | HITS-CLIP / AGO2 |
| Cell line / Condition | CD4+ T cells (C57BL/6) / CD4+ T cells, WT, biological rep8 |
| Location of target site | NM_001077202 | 3UTR | UUACAGAAAAAAAAA |
| Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
| Article / Accession Series | PMID: 23142080 / GSE41285 |
| CLIP-seq Viewer | Link |
39 mmu-miR-1194 Target Genes:
Functional analysis:
ID |
Target | Description | Validation methods |
 |
 |
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| Strong evidence | Less strong evidence | |||||||||||
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 |
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 |
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| MIRT577399 | Usp29 | ubiquitin specific peptidase 29 |  |
|
2 | 2 | ||||||
| MIRT578645 | Gtf2h2 | general transcription factor II H, polypeptide 2 | |
|
2 | 4 | ||||||
| MIRT583791 | Elf1 | E74-like factor 1 | |
|
2 | 2 | ||||||
| MIRT585587 | Tox4 | TOX high mobility group box family member 4 | |
|
2 | 4 | ||||||
| MIRT585769 | Spg21 | SPG21, maspardin | |
|
2 | 2 | ||||||
| MIRT586369 | Pctp | phosphatidylcholine transfer protein | |
|
2 | 6 | ||||||
| MIRT586934 | H2-T24 | histocompatibility 2, T region locus 24 | |
|
2 | 4 | ||||||
| MIRT587278 | Fam154b | stabilizer of axonemal microtubules 2 | |
|
2 | 2 | ||||||
| MIRT589358 | Osbpl8 | oxysterol binding protein-like 8 | |
|
2 | 4 | ||||||
| MIRT591418 | Cd300a | CD300A molecule | |
|
2 | 4 | ||||||
| MIRT594881 | Lpar6 | lysophosphatidic acid receptor 6 | |
|
2 | 2 | ||||||
| MIRT594899 | Hs6st2 | heparan sulfate 6-O-sulfotransferase 2 | |
|
2 | 2 | ||||||
| MIRT595087 | Unc5d | unc-5 netrin receptor D | |
|
2 | 2 | ||||||
| MIRT595608 | Jazf1 | JAZF zinc finger 1 | |
|
2 | 2 | ||||||
| MIRT595914 | Syne2 | spectrin repeat containing, nuclear envelope 2 | |
|
2 | 2 | ||||||
| MIRT596713 | Ybey | ybeY metallopeptidase | |
|
2 | 2 | ||||||
| MIRT597013 | Thoc6 | THO complex 6 | |
|
2 | 2 | ||||||
| MIRT597522 | Rbak | RB-associated KRAB zinc finger | |
|
2 | 2 | ||||||
| MIRT598050 | Nip7 | NIP7, nucleolar pre-rRNA processing protein | |
|
2 | 2 | ||||||
| MIRT598082 | Neu3 | neuraminidase 3 | |
|
2 | 2 | ||||||
| MIRT598528 | Itsn1 | intersectin 1 (SH3 domain protein 1A) | |
|
2 | 2 | ||||||
| MIRT598953 | Ggps1 | geranylgeranyl diphosphate synthase 1 | |
|
2 | 2 | ||||||
| MIRT598967 | Gata5 | GATA binding protein 5 | |
|
2 | 2 | ||||||
| MIRT599086 | Efcab4a | calcium release activated channel regulator 2B | |
|
2 | 2 | ||||||
| MIRT599348 | Commd7 | COMM domain containing 7 | |
|
2 | 2 | ||||||
| MIRT599554 | C3ar1 | complement component 3a receptor 1 | |
|
2 | 2 | ||||||
| MIRT600166 | Xpo7 | exportin 7 | |
|
2 | 2 | ||||||
| MIRT600668 | March5 | membrane-associated ring finger (C3HC4) 5 | |
|
2 | 2 | ||||||
| MIRT600971 | Dlc1 | deleted in liver cancer 1 | |
|
2 | 2 | ||||||
| MIRT601455 | Tnfsf10 | tumor necrosis factor (ligand) superfamily, member 10 | |
|
2 | 2 | ||||||
| MIRT601502 | Tmem88b | transmembrane protein 88B | |
|
2 | 2 | ||||||
| MIRT601514 | Tmem151a | transmembrane protein 151A | |
|
2 | 2 | ||||||
| MIRT601558 | T2 | brachyury 2 | |
|
2 | 2 | ||||||
| MIRT601925 | Onecut3 | one cut domain, family member 3 | |
|
2 | 2 | ||||||
| MIRT601984 | Mzt1 | mitotic spindle organizing protein 1 | |
|
2 | 2 | ||||||
| MIRT602669 | Car5b | carbonic anhydrase 5b, mitochondrial | |
|
2 | 2 | ||||||
| MIRT603331 | Slc35d2 | solute carrier family 35, member D2 | |
|
2 | 2 | ||||||
| MIRT605546 | Ppp1r16b | protein phosphatase 1, regulatory (inhibitor) subunit 16B | |
|
2 | 2 | ||||||
| MIRT606291 | Nrcam | neuronal cell adhesion molecule | |
|
2 | 2 | ||||||
| miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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