miRTarBase - #MIRT595083


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pre-miRNA Information
pre-miRNA mmu-mir-677   
Genomic Coordinates chr10: 128085286 - 128085363
Synonyms Mirn677, mmu-mir-677, Mir677
Description Mus musculus miR-677 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-677-5p
Sequence 6| UUCAGUGAUGAUUAGCUUCUGA |27
Evidence Experimental
Experiments MPSS
Putative Targets

Gene Information
Gene Symbol Zfp516   
Synonyms C330029B10Rik, D230016L03, Zfp26l, Znf516, mKIAA0222
Description zinc finger protein 516
Transcript NM_001177464   
Other Transcripts NM_183033   
Expression
Putative miRNA Targets on Zfp516
3'UTR of Zfp516
(miRNA target sites are highlighted)		 
>Zfp516|NM_001177464|3'UTR
   1 AGACACACCTCCAGCGCCCGGCCCCGTGACAGTGTTGACTGTGGCCTCACGGGCGGCTGGAAGGCTCCAGCAGTGACCAT
  81 GGCCGCTTTGGGGAAGAAGATCCAGGGCCTCCTGACCTGATACGTCAGACTCGGGGGCCATTGCGGTAGAGCAGACCCAG
 161 GGACAGCAAAGATGCTATGCCTAGAATTCCGTGTAGCTCTTTCACACACATGTATGTGCTCATCACCCATGGCCTGTGTC
 241 TCCTCGGGATAAGCAGTGGCATTGGCAGGCACAGTGGGAGCTCAAGGAGTCTGGATTACTCAAGACAGGCATGAGACACT
 321 GGAAAAAAGATACTGGTGTGTTTGGAAGAAGTGGACAGCACTGTGGGGAGGTCCAGTGCGTAGGACAGAGATGATGCTGT
 401 CAGGATGTGGGGAGCGGAGGGGGCTGCACTACCGCCTCTCCCCGCCGCCTCAGTGGGGACTTAAAGTAGAAGGTGAGACA
 481 GAGGGGTGTTCAGGTGGTGTTCGAGTGCTGTGGAGAGGAGCTTGGTGAGGCAGGGCAATGAGATAGCTCCTCACGGTTGG
 561 AGTCTGGCCTTGACCCTCTGAGCTGCTTATGCCCCGCCATGCTGCGTCCTCACCGTCCGGGCTGCGTCTGGCTCAGGAAA
 641 CACTGCAGAGGTGGGAACACATATAAATCTGCTTTGGGAAAGAAACATTAGTTGAACACTAAGAGGAGCAGGTGTCTTTT
 721 TTATTCTCAGGACCTTTTTTGTAACAGGGCTACATTCTGCAAACTGCTCACGGAAGGCGACTCCATGTCTTAACAGAGTA
 801 CCGCTGAGTCCTCCCAATTTGGGCCTGTTTTGGTGATGTCAGAGGAACCCTTGAGCAGATTGGGGGCCCTAGACCCTTAG
 881 GGTGCTAGTGCTCTGGAGCCTTCTCTCTCCTCCTCCCACCCCCTTTCCCCTGCACCACCCCAGCAAATAGATGTCTTTCC
 961 CCGTTGGGCCTGGTGAGCACCACCGGATGAGTGACTTTGTTCACAGATTAATGGGGGTCGCCAGGCCTGAGGGTGAAGAC
1041 GGCTTTATATACCTCTTCCTTAGTCACGCAAGTTCACGTTTCTGCGGTGGGGTGAAGGCCCGAGCAGAGGCTGCGAACCC
1121 ACGCGACGTGCGCAGTCGGAACTGTATTCCGCAGGTAGCAGCGATTCCTTTCCCCGTTGCTGTGACACTGTGTGTGATGG
1201 CGATACTTCTGCAAGTTCCAGATGTTTGCACATATGATTTTATGCATTATCACGAATTACCGCTGCCTTGAATGAAAACG
1281 TTCTGTGAAATTTTTTGCAAAAGCTTTACTAGTTTTTTTTTATTGTGAAATTTTGTAAAGGCAGGAAATGGATTAAGACG
1361 AGCATGCTAAATATATTTTTCAAAAAAAAAAGCAATAATTTTACATGTACAGAAATTATCCTAACCTTTAATACTGGTGA
1441 GAGCGACAGTTTACTTAACCCGGTAATGGATCAGCGCGGTTTTTGTAGGAAATGGGCTTCTGATACAAAGACTTGTTTAA
1521 ACACACATGTATACACGAACCCTGGCGCGTGGTTCTCTGTTCTAATGATCTGTTGGATTGTTATTATTATTATTATATTA
1601 TTATTATTATTATTGTTGTTGTTATTGTTAGTTAATGTTTGATTCCAGGTTCTATTTGTTACTGTTTTAATTACTTGACC
1681 GTTCAGGTGACTGCTGCACTTGCAAGCAATGCAATGTTAACTGGCTAGCAGATATATATATATATATACATATATATATA
1761 CACACATATATACATATATATATATATATGTGTGTGTGTGTGTGTGTATATAATTTTTTTTACTTACTTTTCCTGATACT
1841 CCTACAGCAGGTTTGTATGAGAGCGGCTTGCCGTGTCGGAGCGCTTGGGAATAGCCATCATTCTGAGGCGTTACTTGGAG
1921 CAGGAGCAGGTCGTAGAGATACATTCCTCTCTCCGCCTGAGGCAGCGTAGTACCTTTCTGTTGACGGCGTTTGTTCTTTT
2001 TGCTAGGTTCCCCATCATTTCTGCGCTCTCTGGCTTTTTGTTCCCTTGGCTGAGAGTGAAGGTGAGAGTTAGGAATGTCA
2081 GAGCCTGTGATTCGGCCCATCTATTTCTGTGTTTTAGTTATTAAACAGTTCTGTACACAAAGGGACACGAAGGTGTCGTC
2161 TGTGTTTTTCCTGTGTCTGAGGCTGCACTTGGCATTGAGAACTGGAAGGTCTGTCTGTCCCATGAGTCTCCTTCCCAGGG
2241 TCTGGCGGGGAAAGACGGGTGCCTGAGGTCCGTCTGCCTGCCTGGAGAGCAGCCAGTCTCAGGATGACCTGCCCCTCCAA
2321 GCACAGAGAGGCACAATTTTCCTGTAGCCACAAGGCCATGAGGTCCCCAGCGCTCACTCCTGGGACCAGCTGTGTGCTGG
2401 CCTGCTGCATGGTCTCTCTGTCCCGCTGGCCATCAGTATTGGACTGGTTTGTTAATTTGTCTATGTCAGCTCAACAATAG
2481 TATTTAATATGATTTGTGTTTCCTTATTTATTTAGCCAATCTGTTTTGCTTTGCCCCTCCTCCTGTGGTGATTTCCACAT
2561 CTCTGACTGTTACGGACCTCCTACCTACCTCTCTGCATCTTCTGTCTCCTTGTTTCCTGACGGGGTTTTTACAGTGTTGC
2641 GTCTAATGTAACTTAGACAATAAAGGGTTTGGTTGTCTACACTGCAGCCGCTCACTCTGTGTGTCCCCTGCCCTCTCCAG
2721 CTGCCCTCTTCTGACTCCTGCCTCGCTCTCTGGCTCACACCAGCCCAGCCCCCACCATCCCCTGCTTCACTGTGTGACGT
2801 GTCTTCTCTTACGCTTTTCCTCCTTTCAAGTACACATTACACAGAGTTTCTCAAATACTTCAGGTGATTCACTCATTCTT
2881 AATTCCCAGAATGAAGGTTTAACTTCACGAACAATAAATACATTGTGGACTCTTCTCCTCTGGGGAGTTGCTCACTGCTC
2961 CTTTCCACACTTCTGCTGGTTCTTGAAGTCTATGCAGTCTTAGGTGGAGACAGTTTAGCCTCTCCCGCGTAGTACAATTT
3041 CCAAGTCAAGTGGACTTTGCCTTTTTGGTTCTAAAAAGTATTAGATATTTTAAAAAGTTTCTCCAATATTTGCTTATTTA
3121 TTTTTTTAAGTCCAGGAAGACACTGGTTTCTGGAAGTAAGGTGTTCCAGGCGGCACAAGGCAACTCTTTCAACGTAAAAG
3201 AGCTCTGCTGTCCCAGCGGGCTGTAGGGATGCCCCTTCCCCTTGGCTGCTGTGTGCACACCACGGCTTCTCTGCAGCTGG
3281 GACTGGAGTCGCTCTCCATTTATGCTCCACAGGTGACCAGTTCCACCGTGGGCATCACAGGGCTGGCAGCAGTGACCTTT
3361 TCACCTCCTGGGAACCAGTCGAGTTGACACCTGTCTGGGTGGATGAGGGTGTCTTCCAGGTCAAACAGTGCCTGCAGTCC
3441 CCCAGGAAGGACCTGTAAGATGAGACACCTGCCTGTCAGCACCCGAAGAACGAGTTTTGTTGTTTTAATGCTTATAAATC
3521 AGGCAACTTCAAATTGCAACTTTTGTTCACTGAAAAAAAAAAAAACCAAGCATGCCCCACAGACAAGGTTTTCCGGTTAC
3601 ACGTTTTTGACACTAATGATTAACACACACTGCAGAGCCCTGGGACATGCCTGAGTGACGTGGCCGCCAAGCAACATGTC
3681 CCTTTTTCACCGACTCCACTAAGCATTCGTTGAGGTGGGAAGATGCAGAAGCCCATAGAGTTTGTATTTCTTTTTAAAAA
3761 CACAATTTATAATGCTATATTTTGTACTCTTTATATTAGAATTTGTAACTAGATTGATGTATTTAACTATTTCTGAGAAA
3841 AATAATTCAATGTTACAGCTGTGTGATTAAAAAGTATTTAGATAAAACATATTCACTCTATTGGAATTTGAAATAAATAA
3921 AAACATCTTGGAGTTGTAAGACATTTACCCCTCTTTTAGAAGGTGGCTTACATCATTTGACA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aguCUUCGAUUAGUAGUGACUu 5'
             | | ||  |  ||||||| 
Target 5' attGCAACTTTTGTTCACTGAa 3'
 3533 - 3554 147.00 -10.70
2 
miRNA  3' agUCUUCGAUUAGUAGUGACuu 5'
            :|:||:|:  | ||||||  
Target 5' tgGGGAGTTG--C-TCACTGct 3'
 2941 - 2959 131.00 -12.30
3 
miRNA  3' agUCUUCGAUUAGUAGUGACuu 5'
            ||:  ||| |::|:||||  
Target 5' ccAGGTTCTATTTGTTACTGtt 3'
 1645 - 1666 128.00 -12.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0003451
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013594. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' agucuucgauuaguAGUGACUu 5'
                        ||||||| 
Target 5' --------------UCACUGAa 3'
 		1 - 8
Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
44 mmu-miR-677-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT580133 Ubap2l ubiquitin-associated protein 2-like 1 1
MIRT582833 Itgb3 integrin beta 3 1 4
MIRT583171 Grik3 glutamate receptor, ionotropic, kainate 3 1 3
MIRT583782 Elovl7 ELOVL family member 7, elongation of long chain fatty acids (yeast) 1 2
MIRT585951 Sin3a transcriptional regulator, SIN3A (yeast) 1 1
MIRT589768 Iws1 IWS1, SUPT6 interacting protein 1 1
MIRT591164 Mup19 major urinary protein 19 1 1
MIRT591170 Mup12 major urinary protein 12 1 1
MIRT591176 Mup10 major urinary protein 10 1 1
MIRT591807 Mup14 major urinary protein 14 1 1
MIRT592567 Mup17 major urinary protein 17 1 1
MIRT592608 Mup11 major urinary protein 11 1 1
MIRT593180 Clasp1 CLIP associating protein 1 1 1
MIRT594028 5430427O19Rik RIKEN cDNA 5430427O19 gene 1 1
MIRT595083 Zfp516 zinc finger protein 516 1 1
MIRT595535 Fign fidgetin 1 1
MIRT595555 Ubr3 ubiquitin protein ligase E3 component n-recognin 3 1 1
MIRT595557 Tcte1 t-complex-associated testis expressed 1 1 1
MIRT595562 Sptbn1 spectrin beta, non-erythrocytic 1 1 1
MIRT595569 Nrsn1 neurensin 1 1 1
MIRT595571 Mtf1 metal response element binding transcription factor 1 1 1
MIRT595579 Josd1 Josephin domain containing 1 1 1
MIRT595580 Fam101b refilin B 1 1
MIRT595587 4930444A02Rik protein-O-mannose kinase 1 1
MIRT595593 Ago1 argonaute RISC catalytic subunit 1 1 1
MIRT595602 Nbl1 neuroblastoma, suppression of tumorigenicity 1 1 1
MIRT595604 March8 membrane-associated ring finger (C3HC4) 8 1 1
MIRT595625 Nol7 nucleolar protein 7 1 1
MIRT595629 Gnptg N-acetylglucosamine-1-phosphotransferase, gamma subunit 1 1
MIRT595633 Ednra endothelin receptor type A 1 1
MIRT595649 B3galt5 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 1 1
MIRT595667 Rxrb retinoid X receptor beta 1 1
MIRT595743 Ctdspl2 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2 1 1
MIRT595789 Fignl2 fidgetin-like 2 1 1
MIRT595880 Gid4 GID complex subunit 4, VID24 homolog 1 1
MIRT595909 Tapt1 transmembrane anterior posterior transformation 1 1 1
MIRT595919 Trim9 tripartite motif-containing 9 1 1
MIRT595925 Efr3a EFR3 homolog A 1 1
MIRT595937 Acmsd amino carboxymuconate semialdehyde decarboxylase 1 1
MIRT595943 B3gnt5 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 1 1
MIRT596004 Disp2 dispatched RND tramsporter family member 2 1 1
MIRT596008 Ceacam1 carcinoembryonic antigen-related cell adhesion molecule 1 1 1
MIRT596167 Ms4a15 membrane-spanning 4-domains, subfamily A, member 15 1 1
MIRT597273 Slc25a33 solute carrier family 25, member 33 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-677 N-ethyl-N-nitrosourea NULL 12967 Quantitative real-time PCR mouse liver 21029445 2010 up-regulated

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