miRTarBase - #MIRT595113


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pre-miRNA Information
pre-miRNA mmu-mir-664   
Genomic Coordinates chr1: 185242975 - 185243043
Synonyms mmu-mir-664, Mir664
Description Mus musculus miR-664 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-664-3p
Sequence 38| UAUUCAUUUACUCCCCAGCCUA |59
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Rgs17   
Synonyms 6430507P11Rik, Rgsz2
Description regulator of G-protein signaling 17
Transcript NM_001161822   
Other Transcripts NM_019958   
Expression
Putative miRNA Targets on Rgs17
3'UTR of Rgs17
(miRNA target sites are highlighted)		 
>Rgs17|NM_001161822|3'UTR
   1 TTTTCAGTTGACAGGCCAAAAAAAAAAAAAAATCCATTTCAGAGGGCTGGGATGGGAAATAAAAGTAATTAAATAACACC
  81 AGAAATTGAGTTCCTGAAAAACTACAGGTTGACTGCTGGGGAAATACAATGAGGAAGGTCTCTTGTCTCCATTTTTATCA
 161 AGGTTATCCATGATTCTGTTTGGGGCAACAACAACAAAAAAAGCCTATATGAGACAATGAATTGCCAAATTAAGTTTGTT
 241 TGATTCAACACTTGCTCTACTTGCAAGCAAATTGTGTTCCTAGTCCTCTGAACAATCCCCTAGTGCATCTCTAGAGGCCG
 321 CATGTGCAAAGTAAAACTGCTTCGTCTGCCCTTTACTTGTGCTATTAAATCAGTAGCATACCTTGTATCTGTATTTAAGG
 401 ACTTTTGTGCAATATGGTCTCTTAGAAACAATTGCCAACAAATTGGCCGTGGTTTGCATTTTTTAAAAGCATAATCCAAT
 481 ACACACAAAGGCTATTTTAAACATGTAACAGTGATATTTAACCATGCTACATACTGTGTTTAGTATACCGTCTCTGAAGC
 561 CAATTTTTCTGTACATGTTTAAAAAAAATAGAAGCTGTTAAAGGATCTTACTGTGTGAGCCCTCTGGTTGTTCACTGCCC
 641 AAATTCTGGCATTTTTGTTACAGAAGAGTTTACTATATAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA
 721 GAAAGAAAAGAAGAGAAAAAAAGGAAAGAAAGGAAAAGAAAGAAAGAAGGAAAGAAGAAAAAGAAAGAGAGAGAAGACAA
 801 AACTTTATGATAAGAAAAATTTTAATAAGCACAAAACAATCTGCAGATATCCTGTCTCATTGAAAATTCCAAGAGTGATA
 881 CTGTTTTTAGGTCTGAAAGAAGAAAAGAGTCTATGTATATATGAAATGTCCAGTACTATAATTGAGTTATTAAAGAATGG
 961 CTCCTCAGTTCTAAAAGAGTGATGCAAACCTCTGAATGTCAGCAGGTAAACAATAATAAGTTCGATAATCCAGTTTGGGA
1041 ATATAAAGGGAAAAGAACTAGTAAAGGAATGCCTACATGTAGCACTGTCAAAAGCATAGTGAGGAATTGCATCTGACTTG
1121 TGGCGTATTCACCTTGAACATATAATCCAGTGAAAAGCTTAAGTTTTTCCTAAGTAACACTTTATAAAGAATTACAAAGA
1201 AGAAAACCTTCAATGTCAGACCTTAACTGTTTTCACATAGTCTCTCAAGAAAGACTTATTTTAACATTGTTAAAAACAAA
1281 CTCTTAACCCTCATCCATAATTAAAAGGCAGTACAGTGCTCAAACCTTCTAGCTGCCAATATTATTTTTTTATAATTACA
1361 TTAACTCTTTACATGAAATACTCCCCTGACCACAGCATGTATTTATGACCTTCTGAACAGTATCGTCTTGTGCACAAAAC
1441 CAAAATTACAATCAATGTCATATTTATATCAAGGTCTTACCTTTCAATATCAAAATCCCCTGGAGCCTATTTTTTTAATC
1521 TTAAACTCAATGAGTATATGTTGAGTAACCTTTAAAAATGAAAGATTTAAAAAGATTTATATCCACTGAGAATACAGGCC
1601 TTATATTAAGGATGATATTAAAATTATAGGTTTTATTTGTCATTTTTAACTACCTCTCATATTTTTAATTTATTAAACAT
1681 ATTTTTTTAAAGCACAAGTTTGGAGTTTTCTTTTTAACTTTATTTTACTTGAAAGGTTGGCATCATGTTTCTTTTTCCAT
1761 AAATTTAAAACATTTCAAGAATTGTTTTTTAAGTGAATATGATTTTCTAATTTATATAGAATTCAGGAGCTATGATTCAA
1841 AGGGCTATTACATATTCCCTATGCAAATATTTTAACTGTTGCAGTGTTTGACAAAATGGGATTTTAAAAAAACAATTTGC
1921 AGAGTGGAAAAACAAAACGTTGTGTTTACACTGGCGCCGCTGACTATCCTACCATGCTCAGCACTTTTAAATACATTATT
2001 TATGACAATGTAGTTTAAATGCAAGATATTTATAATATTCATCTTTTAAAAAATGCCTGTGGATTTTTTTTTTTCCTGAA
2081 AGTGTTAGTGTGTTTAAGATACGCTTTGCTTATGTCACCTAGATGTTTACTATTTGAATAGTTTTGGTAATCTGTTGAGA
2161 AAAAAAGATAGTTATGAAGATGTTTACAAAAATATAATCCTATTTTTTCCTAAAGAGTCAAGAAAACGTAAAAGCATCAT
2241 GGCTTCTATTGTGAAGTCCCACCGTTCTGAATGATTTGATTCTTTCCTTGATTAGTGTTGATTATGCTAATAGGATGTAT
2321 ACACATGTATAGTGTACCATCATCCTCGTTCTGTGTTTGGGGCACAATGAGTTGGTGCACATGACCAGGAAAAATGTAAT
2401 ATATTACCACACCCTGCATGCATGATTGTTTTCGATGTAGACTTTATTATATCCCTGTTCCATGGATGCCAGTGACTGAC
2481 TGTCACAGGCGATCTTTACAATACGCTCTGGACTATTGGGCGAGCTGTCTGTGTGACAATGCATTTAATATTTTACCAAA
2561 TGGAAGAGATAAGGCTCCTTGAGAGAGTCCTCATCTCTAACCTAGGCATTTGCAGTGTTTGATTTCAAAGTTGCTGTGTC
2641 TTTGGAACCTGTGTACAAGGAGGTTCCAAAGCTGATTTGCACAGTCCCTGCCATTAGCCATTGCTATGTGAGCAGTTCTA
2721 TGCTAAATTCATATTTCAGGGGGATGGCTTCTTAGTTCACTTGAGAATGTTTCAAGTTTTGAAGAGTGTGTCTGTGGACT
2801 TGTGACTATGCCAGTCTTTAAAATATGGTGTGGTTTCTTCTGTTTAAGTCACAGAGCTATAATAAACAAACAAAAGCCCA
2881 CAACTGGTGTCTGCCACCATTTCAAGAGCACAACACTTGATATAGTCAAGTTTGATTTAATTCCTAGTGGGTGAATCGCT
2961 CCCTGGGGAGACCCTAGCACTTTAAATGAAGCACCTTATATTTACGATTGCCAAGATAACTTTGCATATTGTCAGAATCT
3041 GTTAATGCACACACAAGAGGATAACGCACTTTCTATAGTACCTGTCTATAGGCATATCTATTGTGGAAAGCAAAGGAGAT
3121 TGTGTAGGTGATGTATACAGCGGTGACTCAAAGGCAGATGTCTTCATAGGTGGATCTTACACATAGACATGCTAATGCTG
3201 AAGGGGTGACTCTTCTCTTTCTGACTTATTATGACATCATCCAAAGTTAGCACCGATGCTCTGATGGCATGAAAAGGGCA
3281 TATGAATGTTAACAAAACAGGATTTTGATACTGAAGTTATAAATGTAAAAGTAAAAATTACAATATGGCCTTTAAAACAG
3361 TCTTACAATATTTATATATGACCAGTTCTAACAGTGTGTCACTGCCTAGAACATTTATTAGTGCCCTTCAATGTCTCCTA
3441 GTATACAGAATGTGCTATATTTATTTGATAGTTAAGTGTTAAAGGACTATAGAAATAGATAAAGGCTTATCTGTTGAAAA
3521 CAAATGTACATCTCTTCCCATTATGCTAATTTAAAATACTTGAATCTTAATTTAAGTTCATTCTTTTATTCTAGAATTTT
3601 TTTAATAGTCTCAGAAAAATGTCATATTGTAATTGTGGAAACTACACTTGCAACAAATAAAAAACTTCAGGTGCTTTGGT
3681 GAAGGTTTTTAAGTGGAAGGAATGAAAATAAAGCATAGAAAAATGAACTTTTTGAAGAACCATCAATTTGTTTTTAAACA
3761 GGTAGAAGATACACCTAGAACCCGACATAACATGAATTTTTGAATACTTTCAGCTTTTATATATTGACATATTATTTTGA
3841 AACAGGAAATACGGTCAACAGATTGGGTTACCTATCTATTTAGATTGGCAGTTAATGACCAATCTCCAAAGTATCTATTT
3921 TTAAGTCTGTTTGAGTACTCTTTTCTGTTCTTAAATTTCTGATGATCATAAAATGCTGTCCCTAGAAAGGAGCATACCAT
4001 GTGTAATAATCTTAGCAATTCTCCACTTGCCACAATTGCAAATATTTACCACTAAAACCTACCAAACCTTCCTTCAGAAC
4081 ATGTCTGCAAGATTATAATATCGTCTTAACATCTTTGCAGTGCACACAGTGCATGGAGGAGCACTGGCTTTGATTTCTGT
4161 GGGTGTTAAAAAATATTTCTTTTACAAATAAAGTCCTAAGCAGCCATAAGGGACATGTTTTCTTTCCCACTGATTGAAGA
4241 GACTATACCAGACCATCATTCTGTAGAAAACAGGGAGATGGAGTGTAGCCTTCGTTGGGCAAATGGGGTTGTGGACTCTG
4321 ATTCTCATGACATAAAGCTCTAAGTTGAGAAAAAGCCATGACCTAAAGTATTCGTGATGATAGCCACATGTACTATTTTA
4401 ATGTCGTGCATGAAATCTGAAATTCAGTCCCCTCCTCATGTCTTATATCGTATGTCTTCTGTTGCAAAGTTTTCCATTGA
4481 TTCGTGCCTTTATCACAGCATTTTCAGCTTTTTAAACAGTTAGAAATATGTGAGCTTTAAACCGATGATTAAACAAACTG
4561 TCTAAGATTTGGGCACATTTCCTTTTGAAATGACAGCTGATTTCGAGTTGTATTTCTATCCAGAGGTGGTAGGGAAAGTC
4641 ACGTAACCTTTCTGAATCTGAATCTGTTTCTTTGTCTTAAAATATTGACTATGGGGTGTCCATGGGAACAGTAATGTTGC
4721 ATATAATAATTGACCGGAGTTCTTCAGCCTAGTAACAATAACATTTTTTGGAAGGTCTGTAGTTGGTGTCATTTCACTGT
4801 ATTCTCTCCTCGTGTTTCCTTGGAAAGTTTAGGTTAGCACACAAAGAGGGTTCATCATGACATTTTCATACCAGTGTCTC
4881 ATGATATCTTCTTCTCATTTGTTCCTCACTGCCTTTCCCAGGGCCTTCTACTTTCTTTGCTTATTTTTTTTTAAACATAC
4961 TTGAAACTTTAAAATGTGCATAGGTATGGACAAACACCATACACACACACACACACACACACACATGCATACAGAGACAC
5041 ATATACACACAGACACAGACATACAGTTATGCCGCCCACAGACACATGCACAAGCACACATACACACAGACACATCGAAA
5121 CATCCACACACACCACACACACACATACAGAAATACACACTCAAAGATGGAGACATAGGCAATAAAATGACCATCCCTGT
5201 GGGTGGGAATACTATAGAATCAAGTTAGTTACCAGTATGCCAAGTATGCCAGTGGGCAACAGAGCTGAAAGCAATGTCAT
5281 AAATCATGATTCGAAAATAATCCCTTCAATATTAGTGTGACTTTGTCGATTCTATCATGAAACTATGAATAGAAAGATGA
5361 ATACAAGTGGCTAAGTGTTCATTAACAGTTTCACCAAACTTTTGAATATTTTCAGCTTTATAGCAAAATGATGAGGCAGT
5441 AAATGCCCTCATGTGTTCTATGTGTCTGGGAAGCAATTTTCCCAAATAATAACTAAAATTGACCCATCGAGTCTGTGTTC
5521 CACTCCATCATCATCCTTCACCTCTTGCCTTCTCTGCCCTTCCCATCCTGTAACCACACGACCTCCTGTTGTTTCCTCTC
5601 TTTGTGTTTCTTTCAGCTGTGACTCTCTCTAAATGGTCTTGTTTTTTCAAAACCTTGACAGTTGTGAGTGGAAGTGATGT
5681 CTATTTTACAGAGCATCGCTCTGCAGGGTGGGGTTTGTCAAGACTTCTCCTGTTTATATGAGTGGGGATTTTTTTTTTGT
5761 TTTTGTTTGTTTGTTTTTAGTTTCTGAAAGGAATAATGTTATTCTGTTCTTTCTATCATAAATATTTGTTTTTATTTTAT
5841 GTGATTGAGTGCTTTGTCTGCATGTATGTATGTGACCACATGTGTGCAGCACTTGAGGCCAGAAGAGTCCTCTGGAATTG
5921 GAGCAACAGATGGTCTTAAGCCTCCATATGGGTTCTGGGTACTGAATCAGGGTCTTCGGTTCTCTTAACTGTTGAGCCAC
6001 CTGTCCAGCCCCGAATAGCCAACCTTATTACATGATATCTACTTGCCACTAGGGTAAGTACTCTTGCTTTGGGCTCCTCC
6081 TTCCTATTGCTGTTGCTGTTCGCCCTGTCACCTGATTTAAGCATTGCCTGTTGGGTACAGCCTCTGCTGCCAAGTGATCC
6161 ACATGCAAAGGTGTCTTAGAGGGAACTAGACCCTGCATTTACATTGAGCAAGAAAAACAACCAACCCCACCCCCCATAAA
6241 AACAACTCTGGCCAATTTCTTAGCATGAATACTAGGCTCCATTAAAGGATTACTAATAAATACTATAAAGAAAATGCAAT
6321 GGATTAAGAAGTGAAATAAGTCCGAAGCAGTGTTAGCTTGGCTATCTACCTAATAAAAGTGCAACTGTTCTCTCTTCCCT
6401 GAAGCACCCTGCATACTTATCTAGTTCCTGCACACATGCTGGATTTGAAGATTTTAGCAAGAGGTTATTTATTACTCGTG
6481 TTTAATGCCTTCCTATATACCATCCCCTGAGCAATGTATACAATGCTGAAGTATAGAAAATTTGATGTTGTTCCTCAAGC
6561 TTTAACCCGTTTGTCATAGTGTTTCTTGTGTGTGTTTTTAAAGAGTTTGCAAAATACTAAAAAAAAGTATTTTAATGAAT
6641 TTGATTCTGTGAAATACATCATTTTGAACATGTATAATTTCTTACTTTCATGCTCATTCTATTTAGTGATCTATTTCCAT
6721 ATTTTGTATTCTATCAATATATTTATTAGTGGACCACAAAAAGGTTCCGAGTACATGAATATAAGAGTAGCCCTGCTTGG
6801 CTATTTTATAATACCAAAATACCTGTGGGTACTCATAGTTGAATAGATACTCCAGATTCTTTTCCTTGCAGCTCAAGTTT
6881 GTCTGGGTTTTTCTTCCATTGAACAGATTTCATCATTTCCCTATGTACAATAGTAGACTTCCTGAAAGTGCTACAGTTTT
6961 TACCATGTAATAGACTTGAGCATGACAATACTGTAAGAAAATACAGAAGTCTCTAAATATGTCTGAGTTTTCAAAGGAAA
7041 TCAAATAATAGGTGTTTTCATTTGACTATATTGTGGCATTTATGGGTATTACTGTAAAATGCATATGCATTACCTGATTT
7121 TCTTAACATGTTTTGAACTAATAAAGAATTCTCCCATGTATCCTTAGACAAGTTATTGTTTGCTTATTGGTGTAATGCTT
7201 ATCTGAGTATAACAACTTCTGGGTGGAGTCTTCACATTGTTATGTAACATTAGAGTACCTTTTTATAAGAAATAAAATAT
7281 GTTCTTGAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' auccgacccCUCAUUUACUUAu 5'
                   |||  ||||||| 
Target 5' agcctatatGAGACAATGAATt 3'
 202 - 223 149.00 -6.50
2 
miRNA  3' auccGACCCCUC------------AUUUACUUau 5'
              ||||||||            ||||||||  
Target 5' ctccCTGGGGAGACCCTAGCACTTTAAATGAAgc 3'
 2959 - 2992 140.00 -17.90
3 
miRNA  3' aucCGACCCCUCAUUUACUUAu 5'
             |:| |||:   |||||:| 
Target 5' tgtGTTTGGGGCACAATGAGTt 3'
 2352 - 2373 139.00 -13.40
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0012774
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013594. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
34 mmu-miR-664-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577936 Prl5a1 prolactin family 5, subfamily a, member 1 1 1
MIRT578554 Hopx HOP homeobox 1 1
MIRT579006 Cyp19a1 cytochrome P450, family 19, subfamily a, polypeptide 1 1 1
MIRT579669 1200011I18Rik GPALPP motifs containing 1 1 1
MIRT580267 Trim12c tripartite motif-containing 12C 1 1
MIRT580632 Sycp2 synaptonemal complex protein 2 1 1
MIRT580981 Slc12a2 solute carrier family 12, member 2 1 1
MIRT581678 Ppp1r3d protein phosphatase 1, regulatory subunit 3D 1 1
MIRT582092 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1 1
MIRT582126 Nr2c1 nuclear receptor subfamily 2, group C, member 1 1 1
MIRT583126 Heca hdc homolog, cell cycle regulator 1 1
MIRT583594 Fam78b family with sequence similarity 78, member B 1 1
MIRT583662 Fam160b2 family with sequence similarity 160, member B2 1 5
MIRT584399 Cdc27 cell division cycle 27 1 1
MIRT584815 Arid4b AT rich interactive domain 4B (RBP1-like) 1 1
MIRT585915 Slc16a9 solute carrier family 16 (monocarboxylic acid transporters), member 9 1 1
MIRT586057 Rnmt RNA (guanine-7-) methyltransferase 1 1
MIRT586080 Rgs4 regulator of G-protein signaling 4 1 1
MIRT587942 Atl2 atlastin GTPase 2 1 2
MIRT590595 Atp2a2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 1 1
MIRT590735 Ado 2-aminoethanethiol (cysteamine) dioxygenase 1 1
MIRT591492 Aqp3 aquaporin 3 1 2
MIRT594084 Scoc short coiled-coil protein 1 1
MIRT594733 Zfp62 zinc finger protein 62 1 1
MIRT595057 Aldh1l2 aldehyde dehydrogenase 1 family, member L2 1 1
MIRT595113 Rgs17 regulator of G-protein signaling 17 1 1
MIRT595239 Rhbdl1 rhomboid, veinlet-like 1 (Drosophila) 1 1
MIRT595762 Otud6b OTU domain containing 6B 1 1
MIRT595778 Mc1r melanocortin 1 receptor 1 1
MIRT596728 Wdr59 WD repeat domain 59 1 1
MIRT598239 Mlph melanophilin 1 1
MIRT600005 4930563E22Rik RIKEN cDNA 4930563E22 gene 1 1
MIRT602439 Emx2 empty spiracles homeobox 2 1 1
MIRT736865 Smad4 SMAD family member 4 4 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-664 Acarbose Approved 444254 Microarray diabetic rats cells 24260283 2014 up-regulated
miR-664 Sorafenib approved 216239 Quantitative real-time PCR hepatocellular carcinoma 21530512 2011 up-regulated
miR-664 Ginsenoside Rh2 NULL 119307 Microarray NSCLC cell line A549 23152132 2013 up-regulated

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