miRTarBase - #MIRT595160


—
pre-miRNA Information
pre-miRNA mmu-mir-350   
Genomic Coordinates chr1: 176772325 - 176772423
Synonyms Mirn350, mmu-mir-350, Mir350
Description Mus musculus miR-350 stem-loop
Comment Kim et al. cloned 40 new miRNAs from rat E18 primary cortical neurons .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-350-3p
Sequence 61| UUCACAAAGCCCAUACACUUUC |82
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Cbfa2t3   
Synonyms A630044F12Rik, AI465270, AW229127, Cbfa2t3h, ETO-2, Eto2, MTGR2
Description core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human)
Transcript NM_001109873   
Other Transcripts NM_009824 , NM_177289   
Expression
Putative miRNA Targets on Cbfa2t3
3'UTR of Cbfa2t3
(miRNA target sites are highlighted)		 
>Cbfa2t3|NM_001109873|3'UTR
   1 CCTCCAGATCTGACACCCAGCCCATGGACGCCATGCCCTGCCAACCTCCTGGCCCCACCGTGGCCCACCAGTTGCCTGGA
  81 GCCATTGCTGCTACTGCTTCTCTCCAAAAGAAAACACAGATCCAACAGAACTGCATCCGTGCAGCCCCAGCTACCTGACA
 161 AGGTCTGCCGGGACCTCTACAGCCTCTCGTCCATCGCAAGCACCCTCAGAAAGCATCGCAGAGCGTCAGACAGTGGCACC
 241 AGCAGGAGTGTGGCCAGCTCCGCCGCTGTCTGTCCCCTCTGTGTCCGTCTCTCTGTGTCCGTCTGTCTTTCTGCGTCTGC
 321 CTCTCTCTGTGTGTCTCTCCGTGTCTTTGTCTCCTGCCGTTTCCCTGACTCTCCGGCGTCTCTGTCTTTGTAAAGTCCAC
 401 ATGATCTCTCTGTCATCAGAGAAACCTAGTTGGTAGCTTTTCTTTTCCATGAAGAACTTTTGGAGATTTTGTTTTGTTTT
 481 GTTGGCAAACAAGCTACTTAGAAATGGACAAAGAAGACTGTGGGGTTCTCCCCATTCCTCATGAGTAAGGGAAGAAACTG
 561 TGATTTTTCTATCCAGAGTTGCTGTATCGCCCAGCCAGCCCCAGGGGCATCAGCGAGCACACAGCAGAGATAATGAGAGC
 641 AACTGCAATTTCCAACTTAAGAAGCAGCTTTTGTTTCAGGTTTTACTCCTTTAATGTCAAACTCTTTGGCTTTAAGTGAA
 721 AAAAAAAAAAAGGAAAAAAAAATCCAAAAAGTTTTTTTTTTTTTTAAGCAAAAGAAACACACCTGTAAACTACCTTGCCA
 801 GCTAGCCAGCCAAGGATGCCAGACACACCTCGCTCCAAAGGAAGCCCAAAAAAGCAAACTCAAGAAATTGAAATCCAAAA
 881 TTTATTTTTATCACTGCCAAAGTATTTTCTTCATGGTCTCTCCGCCCTGGGTTGGTTTGCATGTCAGTCTTTGATTTGGG
 961 TTTTGATTTTGTTCAGGGGGTGTTGGGATGCTGGGATTTGGTGGGTGGGGCCAAGAAGAAACCTTTTGTTCCTTTTTGAC
1041 TAGTAGGCAGCATCCTTGCTGGGCAGAGCCATCGGCAGCAAGGCCACGTGACCCTCAGCACCGCATCCAGAAGCCCCAGT
1121 CAGCGTGAGCCTGAGCGTTGTGTGCCCTAGGGCTCCGTCCATGCGTTCCCTACATGCAAGGGAGGCACCTCCTTCCATCG
1201 GTCCCCACACCTTCCTGGGACTTCTCATAAGTCATTTCCACTTGTGAAAAAAAAAAAAGTGCTAAAGTGCTCTTCCCAGA
1281 GAGAGCACACTTCCTAAACGAAACTGTGACAATCTTTTTGGTTGATTCTTGACTGCTTTTGAGAGCATAAGGAGACAGCA
1361 AATCACGCTTTCTCAGTTTTCCCTCCTCTACCTCTCTGCTCTTCCATCCCACCCTACCTCCATCTCGGCCCCATCACCCT
1441 GCCCCCAGTGTCTTGCTGTGGCTACCCTCATTTCTTTCAAACTTTTACAGAAAAAAAGGAAAAAGAAAAAAAAAAAACAC
1521 GAAAGCCAGCAGCCACCCGCACCTCACCCGGAAGACTCCAGCCCGGCTGTACATATCCCTGTGTTGTATCCCCAATGTTG
1601 GTCTCCAGTCCTGGGGGTGACGGTGATGCTTGCCCATCTACACTCTAGCCCCTGCCCACCCTGCCCTTCGCCCGCTCGAC
1681 TCCCTCAAGGATTCAAGGAGGAAATGAAGGAAGAAGGCTCATGCCTGTACCAAACAAAAAGAGACAGCAGACTCAGTCCA
1761 GGTCTGCACCTCTGTTGTGGCTGCAGCAAGCCGCAGGCCTAGCGTGCAATATGGGGGATTGCTCCTGAACCCTGGGCCCG
1841 GGGGGGCCCTGCCCAGAGCAAGGTGTGCAGCGTGTGTTAATGTGAATGTACATAGTCTCCACAGAGGTCCCAAACCGTGG
1921 CATCAGTACCGAGGAGATGTTTGCCAAATAAAAAGAAATTAAGACAAACCACGGAAGTCTGTGAGGTTTAGGAAACGCTT
2001 TGGAAGGAGTCTGAGTCCTATAGGCTTCTTTGGTGGCTGGTGTTTCTTCACAGCTGTATGACCATTGTTTAACAGAACGT
2081 TCTATGGATTCTGTTGGGTAGCCCGGAATGGAGCCTGACAGCAACCCTTGCTCGGAAGGCTGGGGTAGATCTCATTCCCT
2161 GGAAGGTTCCCGCGGCCGCAGCCCTCCAAGGCCGCAGAGATGTATTCTTGTGGGTTTCAGAGAAGCCAGGACACAAGGCG
2241 GCCTCCTGCCTGCCCGCCCTTCTCTAGTGGACCAGTCCTCCTGAGCGTCTGCCTGCAAACACTGAGTCTGTGTGCTGGTA
2321 GAGCGAGACCTGCCTCCCGTCGCAAGGAGCATCACTGGATTCCATTCCCAACTGTACCCCATGCGGTACTTCTTGTCTCT
2401 GCTACTCCACTGCATGCAGCAACCTTGGCCCTTCCAGAAGGGCAGGCCAGTGACCACTGGTAGGAAAGAGCTCTGGGTGA
2481 TGGGGTGGTGAGCACCATGTTGGGGGGGGGGGGGAACAAAGCTAAGCCCCTCAGGTTGTGCTTAAGAGCTGTAGTCTCTA
2561 GAAGTCCGGGCCAGGAGTCCTGAGGTGGCATTCCTGATGACCTACGTCCCTGGTGTCAGGACTTCGGAGCACCTGCTCCT
2641 ATGTGGTCCAGATATTCTGAGGTAGGTCCCAAAGGGTCACCAGTTTGCTTTAGTTCAAATACCTCCTTTCTTACCCTTAC
2721 CAGCCTTACCCACCTTTTAGGAACAGAGGGCCCACCACAGACAACAAAATGCTGCCCAGCCAAATTCATCTCCATGAGCC
2801 AGGTTACATATTTAGGGTTTAGCAGAGACACCACCCCCCCATCTATACTCTAGCCGTTGGGAGCGACAGCCCCTCAAGGC
2881 GGCTGTCAGATGGCATTGGACCACAGTGAGTCTCCTGTGCATGGGATTTGACTAGCAATTGTAGACATCGAGGGTGAGAT
2961 GCCATGTCTGCTAGGCAGAGCTCAGGAGTCAAGGCAAGTGCAGAATCAGGGTGGCAGGGAGATGGGGCTGGAAAGGGTTA
3041 GGTGTCGCCTGAGGCAAGGAGGCTGGAGGATAAGGTGGGGTTGCTAAGCCGGGCACCGAGGCCCTGGCTAAGCTGTGAGT
3121 TGTGCTTTGACCCCAGCTACCCAGAGACCTCACAGCACAACCAGACAACCTCACAGCTTTGAAGGGACAGGGACCATGGG
3201 TGTTCTTTATTGGGAGGTATACCCTGGGTGGCCATAACCGCAGCCTACCCTTGGTTTCAACCTACAGTCTGCCTGGGGCT
3281 CTCCACAGAGCTGGGGGCGGGGCATGGTACTCCATCCCCATCCCTGCTACCTTTCTCTGGTTCACACACCATGGACCCAG
3361 GCCCTGCCTCCTGGGAACCACAGCCAAGCATGAACCAAACCCCCTAGGCGATGTGGCTCTCACAGAGCCTTGCTCCCCCA
3441 GAGCCTCCAACTCAGAATACCACTTTACTTCCTGTTCTGTGGCCAAAGCTAAACCAGGAAGGCTGAGTAGGGAGGGCAGG
3521 TAGCAGGCAGAGCTCTCCAACCCCCAAGGCCCAGCCCAGATCCCATCTCATGACCTTATGGCTTCTTGGCACAAGTCCTG
3601 TGTGTCCCAGCCCTGCCTCAGACCCAGACTGGCCTTTCCGTGAGGACTACCCAAGAGACTACTTTCCAGCTCAGTCCTAA
3681 GCTCTGCTCAGGAGCGGAGGCCAGTGTTTCTCCTCCCAGGTGTGGTCACAGCTGGATAGGAACTGGCTGAATGACACCAC
3761 AGCTCAGGTCCCACCCACTGCCTGACAAGTGAGAGGAGCCAGCCCCCAGGGGAGCACCCAGAAGGATTCAGTTAACAGGG
3841 ACATCTGTCAGAAGACCTGAGAGGGTTAGCTGGGGATGGAGAAGGCCAAGAATGGGAGGCGGGGCCTCTGTGGGCAGTGT
3921 CACCTTAAGGCCACAGTGGGCAGGGATCAGGATGGGACCCAAAGTTTAGTTTCTCCAAATAAATCATTAGGCTGACACCA
4001 AAGTATCATCCCGGCACCCATCTGTCCTGGCATCCATCTGTCCACTCTTGTACTCCTCAGCCTAACGGACAGATCAACGC
4081 TTTCCTGAGCTCCTCCTTCTGACACGTGCAGCCATCTTCCCATGAACCCGTTTGCCTCTGACCATTCATCATCCTCTGCC
4161 TCCCTTCCAGCCAACTACCCCCATCTATCTATCTTGACCCATTGGCAAATCCATCTTTCCACCTGTCCATTGCTCCATCC
4241 AAACTGTCTCTCCACCCCACCATCCCAGCATACTATACCTACGCCCTTACACCTTCCAGCCCCATGCACACAACCATGTT
4321 CCCTATCATCTCTCTATTCACTGTACTCAGAGTTCTGTCAGCTCCATCAGTGTACCAAAGACACCAGGTCCTCCTACGCC
4401 CTCTACTCTGCTTGGCCTAAACTGTCCAGGGCTGCTCTAGGAATATCTACCCATGAGGCAACAGGCGTCTCCCACCCCTG
4481 GCACTCTGCCCCACCATGGTGTTCAGATACCTCCAGAAATATGAGTTCTTACCGTAGGCATCGTGGGCAGGTTGGATACA
4561 TTCTTGTGGCAGCTGTGACACATCTGGAGCTGCTTGGGGAGAGAAGGGAACAAGAATTGTGCTGTGGGGTGGGTCTGTAC
4641 GCCTGCTCACGAGCTCTGGAAGCCTGGGTTAGGCAGCCACGTTCCCTGAAATTAGGGCAAAGTCCTTGTTTCCAGGAGTC
4721 TGTCCAGAAGAGAGCAAGCCAGGGCTGGCCTCCCTTGACACCGGGGTGCTCTTAGTCCCCTGTGCCCCTGGACTTTTGTG
4801 CACCATTCCCATCTTCTCTGTTAGGATCCCGAGGAGCTCAGAGTTGGACCTGTCCGGTCTGCCCCTCCTCAATCCACGGA
4881 TGGAAAGGAGTATTTGCTGGGTGTTTGGGACCATTCACAAGTCCCCCTGGGAACTCCTTGCTCATCCTGAAGCTCCTACT
4961 ATGTAGGGAAGCTTCTGAAATGAGGCTGGGCAGAGACCCCTGGGTACCCATCATGCTGGCAAGAGGAAGAGAAGATAGGG
5041 AGAGAAGCCTGAGGCGAGCTGGGCTCAGGGGGACTGTCTATTCCTGGAGACAGCTCACAGATAAAGCCCAGGACAGTCAA
5121 CCAACAACTCGCACAAGTCCCCAAATCCTAGAAACCTCTGTCTTGTGTTCTGTTCCTTTTCCTTCCGTTCAGCTGGCCCT
5201 TAGGGTACCAAGCCAGACTGGGAGACAGGAATGCAGCATCTGAGTCAAAGCATGGCCTGCTCAGCACATCCTCCTGCTCC
5281 CAACCCAGACAAGAGGCCCAGGAGCCCTCAGCTCACACCATACCTGGGGTGAGCATAGCATCACACAGCCTGCCTGGGGA
5361 GCCACTGCTTCTCCCCCTACAGGAGCTGGGAGGAGAGTACTGGATGTTTTGTTCATGAAAAGCTGTGTTCCATGCCCTAC
5441 ACATCTATCTGGGTGCTGGGTCCCGCCACCCACCTCTATCTACTGCTGTCCTATATGTTTGCCCTGTGGGCCACGGATGT
5521 GGCGATCAGGTTAATTACATCTGTGTAATAAAGTAAGCATTTGCTACGACGAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cuuucACAU----ACCCG-AAACACuu 5'
               |||:    ||| | ||||||  
Target 5' tccccTGTGCCCCTGGACTTTTGTGca 3'
 4776 - 4802 131.00 -8.20
2 
miRNA  3' cuUUCACAU-----ACCCG-AAACACUu 5'
            |:| |||     ||||: |||| || 
Target 5' aaAGGAGTATTTGCTGGGTGTTTGGGAc 3'
 4884 - 4911 128.00 -12.73
3 
miRNA  3' cuUUCACAU-ACC--CGAAACAcuu 5'
            :|| ||| |||  |:|||||   
Target 5' agGAGAGTACTGGATGTTTTGTtca 3'
 5391 - 5415 126.00 -11.02
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0000605
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013594. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' cuuucacauacccgaAACACUu 5'
                         |||||| 
Target 5' --------------cUUGUGAa 3'
 		1 - 8
Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
27 mmu-miR-350-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT435011 Lars2 leucyl-tRNA synthetase, mitochondrial 1 2
MIRT577176 Tet1 tet methylcytosine dioxygenase 1 1 5
MIRT577951 Pole4 polymerase (DNA-directed), epsilon 4 (p12 subunit) 1 1
MIRT579173 Cd70 CD70 antigen 1 1
MIRT580234 Tsga10 testis specific 10 1 1
MIRT581630 Prkaa1 protein kinase, AMP-activated, alpha 1 catalytic subunit 1 1
MIRT584029 D230025D16Rik RIKEN cDNA D230025D16 gene 1 1
MIRT588424 Zbtb6 zinc finger and BTB domain containing 6 1 1
MIRT588528 Usp45 ubiquitin specific petidase 45 1 2
MIRT588917 Skor1 SKI family transcriptional corepressor 1 1 1
MIRT589679 Larp1 La ribonucleoprotein domain family, member 1 1 2
MIRT590168 Elp4 elongator acetyltransferase complex subunit 4 1 1
MIRT593065 Ehf ets homologous factor 1 1
MIRT593183 BC068281 WD repeat and coiled coil containing 1 4
MIRT595160 Cbfa2t3 core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) 1 1
MIRT596758 Utp23 UTP23 small subunit processome component 1 1
MIRT596783 Ubxn2a UBX domain protein 2A 1 1
MIRT597289 Slc1a7 solute carrier family 1 (glutamate transporter), member 7 1 1
MIRT597494 Rbm41 RNA binding motif protein 41 1 1
MIRT600204 Ube2j2 ubiquitin-conjugating enzyme E2J 2 1 1
MIRT603448 Ric3 RIC3 acetylcholine receptor chaperone 1 1
MIRT604253 Cercam cerebral endothelial cell adhesion molecule 1 1
MIRT604915 Iqcj IQ motif containing J 1 1
MIRT605131 Cnnm3 cyclin M3 1 1
MIRT605214 Arnt aryl hydrocarbon receptor nuclear translocator 1 1
MIRT605697 Ifnar1 interferon (alpha and beta) receptor 1 1 1
MIRT606256 Polr1e polymerase (RNA) I polypeptide E 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-350 Hesperidin NULL 10621 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-350 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) NULL 16078949 Microarray hepatocytes 23107762 2013 down-regulated
miR-350 Tamoxifen approved 2733526 Microarray rat liver 17343880 2007 down-regulated
miR-350 Ursodeoxycholic acid (UDCA) approved 31401 Microarray rat Liver 20689055 2010 down-regulated

Error report submission