pre-miRNA Information
pre-miRNA mmu-mir-186   
Genomic Coordinates chr3: 157544279 - 157544349
Synonyms Mirn186, mmu-mir-186, Mir186
Description Mus musculus miR-186 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-186-5p
Sequence 7| CAAAGAAUUCUCCUUUUGGGCU |28
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Frmd4a   
Synonyms 2700017I06Rik, 5330420J21, AI594879, C230040M21Rik, Frmd4, Gm13190, mKIAA1294
Description FERM domain containing 4A
Transcript NM_001177843   
Other Transcripts NM_001177844 , NM_172475   
Expression
Putative miRNA Targets on Frmd4a
3'UTR of Frmd4a
(miRNA target sites are highlighted)
>Frmd4a|NM_001177843|3'UTR
   1 AGGAGCTACAGTGACAGCTGCTTCCTGGATTCCTCCCTCTATCCAGAGCTAGCTGATGTCCAATGGTACGGGCAGGAAAA
  81 AGCCAAGCCCGGGACCCTCGTGTGAGCCGGCCAGGCTGGATGTGACCACCGCCACGCCATTCCAAGTCACCTCACTGCCT
 161 CTCATTTGCCTTACCCAGATGCACTGTCACCTGCACCAGCTTCGGCCCTCAGCACTTTTCTTCTCCTGTCTCCACATCCC
 241 TTTCACCCTCAAAAACCTGACTGAGGAGACATTCTGGAAGGTTCCGAACCCACTGTGTGTCCCCTGGCTCTCTTGCCCAG
 321 AGAGCCAGACAACAATCCTCAATGGCACCTTGGTGGTTTCCGTCTGCCATGATACCCAGGCCACAAACTGTCAGGAAAGC
 401 CAACACATGCAATTCCTGTAGAAGAGAACAAGAAAAAAGCTAGCCCTGCCTGCAGATACTGAAGCTGTTTCTGGCTGCCA
 481 CCCTATAGGCCGGCCGAGTGCTTCTAGAGGAGAGTGCTCAGGGGATATCAGTTTACACCTGAAATAAATCTGCCATAGTG
 561 AGACAGCCAGCAAGAGCTCCTAAGGGGACGTGTGAGAAAGAAGACTGGTGCTTCAGAGACGCATCAGAGGATCACAGTTG
 641 GTTCTATGCTGCCAAAGATTAGACACATTCAACAATGAACAGATAGGAGGGGCCAAGAGGAACACACTTTTTCTGCAGCG
 721 AAATTTCAATTCTAAACTGCTCCTACGCAGCACAAGTGAAAGTCAACCATCTTAGACCACGTCCTGTCGTGACCCTTCTC
 801 CTGGAGCACTCCTGAGAGCTGGGAACAAACCCAACGGGACAGTGAAGATGAAGGAGGCTCGGCTGGCCTGGCAGACTCGG
 881 ACTCTATCTGTCTGGACATGGGAGGAGCAGGGTACCATTAGGACTGCCCTATGCAGGGGCTTCAGCCTACGCCCCCAAAG
 961 CTTCCTTCCTTTTGGGATGGGGAGGAAGCCAACGGTGACCATAGCCAAAAGCAGTTCTCTCGTTAAAACCACCAGTAAAA
1041 GTGTAACCTTTCTGTTTTGTACAAGGCCTTTCAGTTTTTGTTTTTGGGAAACCAAGGGAAAAAGCACATTGCAATCTACT
1121 CAAGTGTTTTACCGTTGTGGTTCATTTTATGTTTGTTAACACTTGTGTGCACTCGGACAGACCTCGCCTGTGTGTCGCTT
1201 AGTCAGAGAACTCACGTACGCCCTTAGGTAGGAAGACTTTTTACACACGTCTACCAGTTGGCAGGCAGAGCAGAGTTGCA
1281 ACAACTCAGCTCCCAAAGGGCCCAGACCCCTCCAGCAGTATCTGTGACCTCGATACACCACCTCCGTCCACAGATACCAA
1361 GAGGATAAAAGGCAAAAAACATAAAAGCACGCGTGCAAGCTGCTTGTTTACATGTGTTTTGAGCTATGCTTAACACACAA
1441 CCAAAAAGCCATCAATCTCCAAAGGCCTCAGAACCACTTCTATACTAACTAACCAGTGCACAGTCTTAGTGGGGCATGGC
1521 ACTAACGATGGCACAAGAGTCTCTGTGGAGGTAGTGTGGCTTGCAGGGGTTACCTTGTCATGACTTCCTGTGGGGAAGAC
1601 ATCTAGGACCAGGAGCCAGGGTGGGAAGAAAGCAAATCTAAAAGTTGGCTGTGGCATCAAAAGAAAAAAACTTCCTCATG
1681 TATTTAAATCCAAGAATAACAATAGACATTACCAGACTGGGAAGGTAAGAAATGAACAGCAGAAAGGACTGACCCTCACT
1761 TTGCAGTGGCTTGGAAACCTCTGGAAGTCAAGTGGGAGCATTCATCCCATGTGGCCTGAGGCCTTTTCTCTCCCATTCAA
1841 GGCTGGGTACCCCAGAGCTGGGCATGGTTGGCGTGATGAGCCCAGCTTCCAGAAGCAGTACTCTTCCCAGACACTTGAGG
1921 GATAACCCTGTAGGGTGAGTGGGGACGGGATATGATGGTACAAAATGACCCTGGGGCTCCGTGGAGACGGGGCTACCTTT
2001 CTTGCTCCTAGTATGATAGTCGGAGCTACATAAAGCAGGTTTATTTGGGGACTATTTCTTCCTTCCTTAGCTAATGGAAA
2081 TGTGACACATTGTGCCCCATTTTCTTTCTTCCAAAAGGAGATGCCTGAATAATTTGCCTGAACAGAAAACGTCCAAAAGG
2161 AAACAAAGAACAAGACAAGGTTACATGGTTACAGGTTATAACAGAGACAGCAGTTTGAAAATTGATTCTGAAGTCCTTCG
2241 GATCTATGAGAGATGCAGAAAGAATCTAAATGTAATAGATTTGGGGAGCAGAGGGAAACGGAATGGGAGCTGTGAACACC
2321 ATCACGATAGTCTGGCAGTAAGGACAGAGATTTAAGTAAAACAGGTTTTACTGTTTAGCTGTGTTCGGTTAATACACTGT
2401 ACATAATATAATACGCATTAGTCCTTTGAGACTGACATAATAAATGGTCGCTGTGGTTGGTGATGAACGCTCTAGGTATT
2481 GTATCCAAGCACCTGCAGATTCTTTACACATCCCTGTTTCTTTTAAGGGAGATGAAGTCCCCAGTTTAAAGCCCCTATTG
2561 GATTCGTTGGATACATGTAAGAAATTGCTTGTTTCAGCCAGAAGACTATGTTGTGAGCCAGGTTGGTTTATTTTGTTATA
2641 TGCAGGTGAGTGTTGGAGTGTTCAAAGCCCAATTTGTTTTCATCCAGTATTAGTTTAGTTCTAAATATAGCAAGCCTCAC
2721 CCAGGTGCTATCAGATGACCAATTACTGCTTAGTTAACTAGGTGTAAAGTTTTACATATCCATTCATGTCAATAGTTTAT
2801 TACAAGTTGTGTAAAACGGACTCTAGTTTAATAATTGGGGGGGTGGTAAGATTAGGTTGCTCCTGAAACTGACTGTAGAG
2881 CATGTAAAATGATTTTACTGGATTCTGTTCAACTGTAATCAATGACAAAGATGTATGTTGTAGACAAAGTTGCAGAATTA
2961 AAAAAAAAAGGAAATCTGCTTTTAATTTATTCTTTTTGTATTAAGAATTTGTATAGTACCTTTACATTTTGCAGAACAGT
3041 GTTGTCAACACTTATTAAAGCATTTTCAAAATGAAAACAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' ucGGGUUU-U---CCUCUUAAGAAAc 5'
            :||||: |   | || ||||||| 
Target 5' taTCCAAGCACCTGCAG-ATTCTTTa 3'
2482 - 2506 151.00 -8.10
2
miRNA  3' ucgggUUUUCCUCU--------------UAAGAAAc 5'
               ||||||| |              ||||||| 
Target 5' aaaaaAAAAGGAAATCTGCTTTTAATTTATTCTTTt 3'
2961 - 2996 143.00 -6.47
3
miRNA  3' ucGGGUU--UUCCUCU-UAAGAaac 5'
            ||::|  :|||||| |||||   
Target 5' aaCCTGACTGAGGAGACATTCTgga 3'
254 - 278 130.00 -14.40
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0000215
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013593. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
46 mmu-miR-186-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577608 Stxbp4 syntaxin binding protein 4 1 1
MIRT577884 Qpct glutaminyl-peptide cyclotransferase (glutaminyl cyclase) 1 1
MIRT578137 Noc3l NOC3 like DNA replication regulator 1 1
MIRT578249 Mnat1 menage a trois 1 1 1
MIRT579147 Ces2g carboxylesterase 2G 1 1
MIRT579219 Ccdc138 coiled-coil domain containing 138 1 1
MIRT579578 4933427D06Rik RIKEN cDNA 4933427D06 gene 1 1
MIRT580348 Tmem33 transmembrane protein 33 1 1
MIRT580592 Tbl1xr1 transducin (beta)-like 1X-linked receptor 1 1 1
MIRT580668 Stk32a serine/threonine kinase 32A 1 1
MIRT580744 Srek1 splicing regulatory glutamine/lysine-rich protein 1 1 1
MIRT580779 Sp6 trans-acting transcription factor 6 1 1
MIRT580847 Sltm SAFB-like, transcription modulator 1 1
MIRT580944 Slc26a5 solute carrier family 26, member 5 1 1
MIRT581214 Samd4 sterile alpha motif domain containing 4 1 1
MIRT581276 Rprd2 regulation of nuclear pre-mRNA domain containing 2 1 1
MIRT581520 Ptprb protein tyrosine phosphatase, receptor type, B 1 1
MIRT582203 Nfia nuclear factor I/A 1 1
MIRT582893 Ildr2 immunoglobulin-like domain containing receptor 2 1 1
MIRT582996 Id4 inhibitor of DNA binding 4 1 1
MIRT583079 Hnrnpa3 heterogeneous nuclear ribonucleoprotein A3 1 1
MIRT583125 Heca hdc homolog, cell cycle regulator 1 1
MIRT583739 Epm2aip1 EPM2A (laforin) interacting protein 1 1 1
MIRT584127 Crispld2 cysteine-rich secretory protein LCCL domain containing 2 1 1
MIRT584166 Cpox coproporphyrinogen oxidase 1 1
MIRT584531 C230081A13Rik pseudopodium-enriched atypical kinase 1 1 1
MIRT584646 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 1 1
MIRT587978 Armc1 armadillo repeat containing 1 1 1
MIRT588832 Sp1 trans-acting transcription factor 1 1 2
MIRT590758 Acbd5 acyl-Coenzyme A binding domain containing 5 1 1
MIRT593146 Mmp12 matrix metallopeptidase 12 1 4
MIRT593495 Gjc3 gap junction protein, gamma 3 1 1
MIRT594880 Lrig2 leucine-rich repeats and immunoglobulin-like domains 2 1 1
MIRT594898 Ifi204 interferon activated gene 204 1 1
MIRT594932 Gdi2 guanosine diphosphate (GDP) dissociation inhibitor 2 1 1
MIRT594945 Fmnl2 formin-like 2 1 1
MIRT595197 Emc3 ER membrane protein complex subunit 3 1 1
MIRT595343 Frmd4a FERM domain containing 4A 1 1
MIRT595402 Arsk arylsulfatase K 1 1
MIRT595430 A630033H20Rik RIKEN cDNA A630033H20 gene 1 1
MIRT595947 Nelfa negative elongation factor complex member A, Whsc2 1 1
MIRT596153 Opn5 opsin 5 1 1
MIRT601390 Utp23 UTP23 small subunit processome component 1 1
MIRT602153 Intu inturned planar cell polarity protein 1 1
MIRT602577 Colec12 collectin sub-family member 12 1 1
MIRT604817 Nr5a2 nuclear receptor subfamily 5, group A, member 2 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-186 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 down-regulated
miR-186 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-186 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated
miR-186 Ginsenoside Rh2 NULL 119307 Microarray NSCLC cell line A549 23152132 2013 up-regulated
miR-186 Nicotine approved 89594 Microarray Rat adrenal pheochromocytoma PC12 cell 18845019 2009 down-regulated

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