miRTarBase - #MIRT595762


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pre-miRNA Information
pre-miRNA mmu-mir-664   
Genomic Coordinates chr1: 185242975 - 185243043
Synonyms mmu-mir-664, Mir664
Description Mus musculus miR-664 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-664-3p
Sequence 38| UAUUCAUUUACUCCCCAGCCUA |59
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Otud6b   
Synonyms 2600013N14Rik, AU015433
Description OTU domain containing 6B
Transcript NM_152812   
Expression
Putative miRNA Targets on Otud6b
3'UTR of Otud6b
(miRNA target sites are highlighted)		 
>Otud6b|NM_152812|3'UTR
   1 TTTACAAAATGTTACACTGCTTTATAGAGTGTGCCAATCTACCAAGTGCTAGATTGTTGTAAATGCTACTGAAGAACACA
  81 GCTACCTTACACGAGTTTTATGAATAAGCTTCCTAACAGCTTTAAGAAACACGTTGGATTAACTTTAACCAGTGCTCTCT
 161 CAGTGGCAGTTGTAGAACTTAAAGTCTATTGTGGAGGACGTCGTCATCACACACCAAGGTGTTACTCTTCCCGCTAATTA
 241 TTTTTTAGGTGTCCTGCTCATTCTGGCTTGGAATAAATTTTGTTTTAGATTTTGGCCCCCAGCTTTATGTTCCAAAAATG
 321 AAGATTTCAATTTAATTTTTCTTAAAAGTGTGGCGACTCGGTGTCTGTCATCATAGGAACATATTGTCGTGACAGCAGCT
 401 TTATCAGCCTTATAAAGTGCTGGTGATCCCAGTGACCTTGGTGTTACTAGGGTCAGGAGTGCGCCGCTTTTATCAGCACA
 481 TCTCTGCTGCTAAGAGGAAACTACTACAGCAGTTAAGAATTTTTGAATACTTATGAGAATCAGCATAAAGAAATGAATGA
 561 AATCAGTGGTAAACAGGTGTTAATTGTGTCAGTTTTGAATCTTTCTAACAAACTGGAGATCTCCCTTTTATGTATGTGTA
 641 GCTTCCTAAGTCTGTAACTCCTGCATTGCTGGTTTCTCCAGCAGAGGCTGGAGTCAGGGTTGAACCTTGTTTCTCTTCAA
 721 CTGAGAATTTGTATCCTTGCCCCAACACTACTCAGCTGCTTTGCTTTCTCTGGAGCCTTTCCTACTGAGAGGCATCCAGT
 801 GGCCTCTCACTGCTCACCTGTGCAAGGTGATGCTGAAGTAATGCAGAAAGGGAGGGCCATGGCCACACTCGCTTGTTAAG
 881 TCTTCCCCCCTCCAGCTGTCCTAACAAGACCTCTGCTTGTGCTCACTTTCTAGGATTCAATGAAAACCCTGGCTACACAT
 961 TATTTTGTTAAATAATGTATCACTTGTTTTCACTCAAGTGTTTGATTATTATATTTTATGTATAGCAAGAGTTGAAGGTG
1041 GCAGAAGCAGAGAGCTGTGTGGTAAAATCAGTACAGAAGGATTTTATGCGGAATGACTCGTGTTACTCAGATTTTCAATC
1121 ACTTCGAGAAGTATTCTTTGTAACAGAGCTGTGCCCACACACAAGTCTGTTTGCTCTTCTTATGTAACAAAGTGCCATGG
1201 GATGGGGACACAGCTCTAGCAGAGCACTTGCCTGCCATGTGAGGCTCAAGGTTCAGTTTCTAGCACCAACAAAACAACAA
1281 TTTATGAAAATGAAACGTTTTGTTGCAATCTGTTTTTTTCCAACTTAGAACTACAACTAAGTAGAGCATCATGTGTATAA
1361 TATCTGGGCTTTTGACCCATGTATACTGTCTATGAGTAAACACTGGGCTTATGATGTTAAAAGCAGGTAATAGTCTGGCA
1441 GGCTGGCAGAGACTGCTCAGCAGAGCACGGACTTTCTGAGACCATTCCAGCTGACTAAAACATACTCTGCCTGCTATACA
1521 GTGTATTCTGAAGGGTGTATTCTGCTTTGTAACTTTTGATTCTTTTTTTATTCTGAAATTTATAAAAACATAAAGTTGTA
1601 AAATAAATGCTTTGAACCTATTTTGAAGTAATGTTATCTTCATCTTATAGTATCAGTCTTAAGGAGTAATAAAACAAAAC
1681 ATTATGAAACAGTTATTGAAAATTCCATTTTCAACAAAGATACTCTAAAGTAACTCATTAATGTTTTGTTGTTTTGACAA
1761 GGATTCAAATGTACTTAATTATGTATTTCTAATTTTTGTTTATGTTTTATTTTAAAATGGCCAGTATATTTATGAATAAA
1841 ATGTATGGCCCACTTCTACCATTTTAGTGATAAATGGAAAAGTCTTCTAAAAACGGTAGGTGTTAATTTAAGAACCATGT
1921 ATGCCAATTAAATTTGCTGCTGCTGGTCTGATGGAACTTCCACAGCTTTCTTTAGTCTTGATTAAAAACATTTATATCAA
2001 AATTCTAGAATGTGTCTTCACCAAGAATTTTATCTACTTACATATATTTATAGCCTGTAAAAAGTAAAACAAAGAATGTG
2081 TAACTATTGTGAGCAAGATAGCTTTGCTCATAATATCTGATGTTTACCAAGAGAATAAATGATAAAGGAGTTGAATCAAA
2161 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' auccgaccccUCAUUUACUUAu 5'
                    || |||||||| 
Target 5' atcagcataaAG-AAATGAATg 3'
 539 - 559 147.00 -6.50
2 
miRNA  3' auccgACCCCUCAU----UUACUUAu 5'
               |||  ||||     |||||| 
Target 5' taaaaTGGCCAGTATATTTATGAATa 3'
 1813 - 1838 123.00 -9.50
3 
miRNA  3' auccgaccccucauuUACUUAu 5'
                         |||||| 
Target 5' ccttacacgagttttATGAATa 3'
 85 - 106 120.00 -8.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0012774
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013588. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
34 mmu-miR-664-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577936 Prl5a1 prolactin family 5, subfamily a, member 1 1 1
MIRT578554 Hopx HOP homeobox 1 1
MIRT579006 Cyp19a1 cytochrome P450, family 19, subfamily a, polypeptide 1 1 1
MIRT579669 1200011I18Rik GPALPP motifs containing 1 1 1
MIRT580267 Trim12c tripartite motif-containing 12C 1 1
MIRT580632 Sycp2 synaptonemal complex protein 2 1 1
MIRT580981 Slc12a2 solute carrier family 12, member 2 1 1
MIRT581678 Ppp1r3d protein phosphatase 1, regulatory subunit 3D 1 1
MIRT582092 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1 1
MIRT582126 Nr2c1 nuclear receptor subfamily 2, group C, member 1 1 1
MIRT583126 Heca hdc homolog, cell cycle regulator 1 1
MIRT583594 Fam78b family with sequence similarity 78, member B 1 1
MIRT583662 Fam160b2 family with sequence similarity 160, member B2 1 5
MIRT584399 Cdc27 cell division cycle 27 1 1
MIRT584815 Arid4b AT rich interactive domain 4B (RBP1-like) 1 1
MIRT585915 Slc16a9 solute carrier family 16 (monocarboxylic acid transporters), member 9 1 1
MIRT586057 Rnmt RNA (guanine-7-) methyltransferase 1 1
MIRT586080 Rgs4 regulator of G-protein signaling 4 1 1
MIRT587942 Atl2 atlastin GTPase 2 1 2
MIRT590595 Atp2a2 ATPase, Ca++ transporting, cardiac muscle, slow twitch 2 1 1
MIRT590735 Ado 2-aminoethanethiol (cysteamine) dioxygenase 1 1
MIRT591492 Aqp3 aquaporin 3 1 2
MIRT594084 Scoc short coiled-coil protein 1 1
MIRT594733 Zfp62 zinc finger protein 62 1 1
MIRT595057 Aldh1l2 aldehyde dehydrogenase 1 family, member L2 1 1
MIRT595113 Rgs17 regulator of G-protein signaling 17 1 1
MIRT595239 Rhbdl1 rhomboid, veinlet-like 1 (Drosophila) 1 1
MIRT595762 Otud6b OTU domain containing 6B 1 1
MIRT595778 Mc1r melanocortin 1 receptor 1 1
MIRT596728 Wdr59 WD repeat domain 59 1 1
MIRT598239 Mlph melanophilin 1 1
MIRT600005 4930563E22Rik RIKEN cDNA 4930563E22 gene 1 1
MIRT602439 Emx2 empty spiracles homeobox 2 1 1
MIRT736865 Smad4 SMAD family member 4 4 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-664 Acarbose Approved 444254 Microarray diabetic rats cells 24260283 2014 up-regulated
miR-664 Sorafenib approved 216239 Quantitative real-time PCR hepatocellular carcinoma 21530512 2011 up-regulated
miR-664 Ginsenoside Rh2 NULL 119307 Microarray NSCLC cell line A549 23152132 2013 up-regulated

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