pre-miRNA Information
pre-miRNA mmu-mir-677   
Genomic Coordinates chr10: 128085286 - 128085363
Synonyms Mirn677, mmu-mir-677, Mir677
Description Mus musculus miR-677 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-677-5p
Sequence 6| UUCAGUGAUGAUUAGCUUCUGA |27
Evidence Experimental
Experiments MPSS
Putative Targets

Gene Information
Gene Symbol Tapt1   
Synonyms 4932414K18Rik
Description transmembrane anterior posterior transformation 1
Transcript NM_173764   
Expression
Putative miRNA Targets on Tapt1
3'UTR of Tapt1
(miRNA target sites are highlighted)
>Tapt1|NM_173764|3'UTR
   1 GTTTGTGACTAATGAGATGAAGATGTCAGGCCCCGGGCCACAAATTCTGGGTGCCAGGACACTTGCTAAAAATGGCACTT
  81 AAATATTTATTTAAAAACTTAAATTATTAATGGCAAGCAAATCTTAGTATCTTTCTTCCAGTAATGTGGCCTTGCAGAAG
 161 GGCAGATCACGGAGCAGCGGTGGCCTCTGGCCCTGACTCTGGATGCTGACACTTGAACACTTCCTCCTGCGTGGCCAACA
 241 GCAGTGCTGCTTCCAGCACCTGGCCAAGCACTGCAGAAGCTCCTTTCCTTCGTCAGCCTGGGCTTCCAGATCGTCGGGTT
 321 CTCCTCAAGGCCTCTTCAGGATAAGGACACACCTGGGAAACTGTGACGCTTCTACCATGAGTTCCCTTTCCAGCCTCAGA
 401 CTGTCTCCACACACTCTTTCCAGACTTGACTCCATCTGCCAAAGCATTGACAAGTTGCTACATCTAAGTCCAGATGACGT
 481 TCTTACCACCAGAGTCATCCTTGGCAATACATCTGAGCTAGTCAGAACAGAAGGCCACTTAACTACAACGTGGTGAATAG
 561 CAGCGTCCTATGAGAAACAGTGAGCTCTTGGCCCAGTCATTTACTTTATAAAGGTCATGTGTCACTGAGAAATGCTTCCT
 641 GCTGCCTCGGTTACTCAGGGCAGAGTGTGAGCTGTGTGACACGGGCCCAGTGACAATAAAAGGCACAGAGCAATGTCAAC
 721 AGAGTAAAAGGCACAAGTGCAGTGTGACCTGGCTTTGCTTTAAGGCTAATTGCTTCATTGGTTATTCCAAGTTAAAGGAT
 801 TTGGTGTTGAATTGGCGAATAGCCATTGCATAGCTGTTGCTAGTGCCAGGCTCGTTTGTTGGGTTTTGTTTTGGTTTGAG
 881 TAACAGTTTTAAAGTATTCCCAGCAGATTGAGCAGTGTCACCTTGCTGGGCTTGTAAAATGCATTTGTCTGCCTGTGGGT
 961 CTGTTCTGTGTGGGGAGGAAACCCTCACATGACCGACCGACCGTAAGTAGAGTGTTTGGGACACTGTAACTAGCAACCAA
1041 AAGGTGCTCTGGGGTTTTCATCTGAGATAAAGATGTTATTCCTGCTTCATTACTACCTATTAGCATCCCAGGACAAATTC
1121 TCGACACCCTACTTTATCTTAAGAGTCTGTTCTGCACTTCTCTTAGACAGAAAGTTGACCATTTCTGATCTGAACTGTCT
1201 AAAAGATGGTACTCTTAACTTCATTTCCTATGCTGTCTTCTGAAACTCAGCTCTCATGCAAATATTTGGGCACAGATGGC
1281 AAATTTTAAATGGTTTGCTGTATCCTGGATAAGTATAAAACTAGTTTGGATGCTTTGGCTTATGCATTTGAGATTCAGGA
1361 GGGTTTCTAATGTTTTGCACTAACAGAATCTAAAGATAGCTGTTGGAGATACTATATATTTATGCCTTGTAAAGCCAGGA
1441 AGCAATTTTAAGTTTAAAATGAACATTTTTATTCTTTTGTGTATGTTTTAGTGGGGCCCCACTTTTAAACTATTCTTTTT
1521 ACCGAAAGAGAGTGTCCATGGAAACCAGGAGCCGTGTGGAAAAAGCTTTGCCAAACGCAGCCCTGGTCAGAACAAGAGCA
1601 CGACCTAGGTGTATCTTTCTGTTCCGCTTGAGTTTTCTGTTTCCGCGCTGTTCTCTGAAGATTGCATTTCCTTTGCAGAT
1681 GCTCTTGGGAGTGTGGATGATACTCACTTCTGTCATAATGGCATTCAGTACTAATTTTATAAGTGCATCTTGTGTGAAAC
1761 TCAATAAATTCAATTTTATAATCTTTTTTAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' agucUUCGAUUA-GUAGUGACUu 5'
              ||| | || ::||||||| 
Target 5' tataAAGGTCATGTGTCACTGAg 3'
607 - 629 158.00 -11.20
2
miRNA  3' agucUUCGAUUA--GUAGUGACUu 5'
              |:||||||  | |||:||: 
Target 5' tttaAGGCTAATTGCTTCATTGGt 3'
759 - 782 136.00 -13.80
3
miRNA  3' agUCUUCGAUUAGUAGUG-ACUu 5'
            :|:||  || | |||| ||| 
Target 5' tgGGGAGGAAACCCTCACATGAc 3'
971 - 993 128.00 -7.10
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0003451
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013581. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013585. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013586. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
44 mmu-miR-677-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT580133 Ubap2l ubiquitin-associated protein 2-like 1 1
MIRT582833 Itgb3 integrin beta 3 1 4
MIRT583171 Grik3 glutamate receptor, ionotropic, kainate 3 1 3
MIRT583782 Elovl7 ELOVL family member 7, elongation of long chain fatty acids (yeast) 1 2
MIRT585951 Sin3a transcriptional regulator, SIN3A (yeast) 1 1
MIRT589768 Iws1 IWS1, SUPT6 interacting protein 1 1
MIRT591164 Mup19 major urinary protein 19 1 1
MIRT591170 Mup12 major urinary protein 12 1 1
MIRT591176 Mup10 major urinary protein 10 1 1
MIRT591807 Mup14 major urinary protein 14 1 1
MIRT592567 Mup17 major urinary protein 17 1 1
MIRT592608 Mup11 major urinary protein 11 1 1
MIRT593180 Clasp1 CLIP associating protein 1 1 1
MIRT594028 5430427O19Rik RIKEN cDNA 5430427O19 gene 1 1
MIRT595083 Zfp516 zinc finger protein 516 1 1
MIRT595535 Fign fidgetin 1 1
MIRT595555 Ubr3 ubiquitin protein ligase E3 component n-recognin 3 1 1
MIRT595557 Tcte1 t-complex-associated testis expressed 1 1 1
MIRT595562 Sptbn1 spectrin beta, non-erythrocytic 1 1 1
MIRT595569 Nrsn1 neurensin 1 1 1
MIRT595571 Mtf1 metal response element binding transcription factor 1 1 1
MIRT595579 Josd1 Josephin domain containing 1 1 1
MIRT595580 Fam101b refilin B 1 1
MIRT595587 4930444A02Rik protein-O-mannose kinase 1 1
MIRT595593 Ago1 argonaute RISC catalytic subunit 1 1 1
MIRT595602 Nbl1 neuroblastoma, suppression of tumorigenicity 1 1 1
MIRT595604 March8 membrane-associated ring finger (C3HC4) 8 1 1
MIRT595625 Nol7 nucleolar protein 7 1 1
MIRT595629 Gnptg N-acetylglucosamine-1-phosphotransferase, gamma subunit 1 1
MIRT595633 Ednra endothelin receptor type A 1 1
MIRT595649 B3galt5 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 1 1
MIRT595667 Rxrb retinoid X receptor beta 1 1
MIRT595743 Ctdspl2 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2 1 1
MIRT595789 Fignl2 fidgetin-like 2 1 1
MIRT595880 Gid4 GID complex subunit 4, VID24 homolog 1 1
MIRT595909 Tapt1 transmembrane anterior posterior transformation 1 1 1
MIRT595919 Trim9 tripartite motif-containing 9 1 1
MIRT595925 Efr3a EFR3 homolog A 1 1
MIRT595937 Acmsd amino carboxymuconate semialdehyde decarboxylase 1 1
MIRT595943 B3gnt5 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 1 1
MIRT596004 Disp2 dispatched RND tramsporter family member 2 1 1
MIRT596008 Ceacam1 carcinoembryonic antigen-related cell adhesion molecule 1 1 1
MIRT596167 Ms4a15 membrane-spanning 4-domains, subfamily A, member 15 1 1
MIRT597273 Slc25a33 solute carrier family 25, member 33 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-677 N-ethyl-N-nitrosourea NULL 12967 Quantitative real-time PCR mouse liver 21029445 2010 up-regulated

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