miRTarBase - #MIRT596128


—
pre-miRNA Information
pre-miRNA mmu-mir-449c   
Genomic Coordinates chr13: 113035983 - 113036091
Description Mus musculus miR-449c stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-449c-5p
Sequence 26| AGGCAGUGCAUUGCUAGCUGG |46
Evidence Experimental
Experiments MPSS
Putative Targets

Gene Information
Gene Symbol Rgs17   
Synonyms 6430507P11Rik, Rgsz2
Description regulator of G-protein signaling 17
Transcript NM_001161822   
Other Transcripts NM_019958   
Expression
Putative miRNA Targets on Rgs17
3'UTR of Rgs17
(miRNA target sites are highlighted)		 
>Rgs17|NM_001161822|3'UTR
   1 TTTTCAGTTGACAGGCCAAAAAAAAAAAAAAATCCATTTCAGAGGGCTGGGATGGGAAATAAAAGTAATTAAATAACACC
  81 AGAAATTGAGTTCCTGAAAAACTACAGGTTGACTGCTGGGGAAATACAATGAGGAAGGTCTCTTGTCTCCATTTTTATCA
 161 AGGTTATCCATGATTCTGTTTGGGGCAACAACAACAAAAAAAGCCTATATGAGACAATGAATTGCCAAATTAAGTTTGTT
 241 TGATTCAACACTTGCTCTACTTGCAAGCAAATTGTGTTCCTAGTCCTCTGAACAATCCCCTAGTGCATCTCTAGAGGCCG
 321 CATGTGCAAAGTAAAACTGCTTCGTCTGCCCTTTACTTGTGCTATTAAATCAGTAGCATACCTTGTATCTGTATTTAAGG
 401 ACTTTTGTGCAATATGGTCTCTTAGAAACAATTGCCAACAAATTGGCCGTGGTTTGCATTTTTTAAAAGCATAATCCAAT
 481 ACACACAAAGGCTATTTTAAACATGTAACAGTGATATTTAACCATGCTACATACTGTGTTTAGTATACCGTCTCTGAAGC
 561 CAATTTTTCTGTACATGTTTAAAAAAAATAGAAGCTGTTAAAGGATCTTACTGTGTGAGCCCTCTGGTTGTTCACTGCCC
 641 AAATTCTGGCATTTTTGTTACAGAAGAGTTTACTATATAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA
 721 GAAAGAAAAGAAGAGAAAAAAAGGAAAGAAAGGAAAAGAAAGAAAGAAGGAAAGAAGAAAAAGAAAGAGAGAGAAGACAA
 801 AACTTTATGATAAGAAAAATTTTAATAAGCACAAAACAATCTGCAGATATCCTGTCTCATTGAAAATTCCAAGAGTGATA
 881 CTGTTTTTAGGTCTGAAAGAAGAAAAGAGTCTATGTATATATGAAATGTCCAGTACTATAATTGAGTTATTAAAGAATGG
 961 CTCCTCAGTTCTAAAAGAGTGATGCAAACCTCTGAATGTCAGCAGGTAAACAATAATAAGTTCGATAATCCAGTTTGGGA
1041 ATATAAAGGGAAAAGAACTAGTAAAGGAATGCCTACATGTAGCACTGTCAAAAGCATAGTGAGGAATTGCATCTGACTTG
1121 TGGCGTATTCACCTTGAACATATAATCCAGTGAAAAGCTTAAGTTTTTCCTAAGTAACACTTTATAAAGAATTACAAAGA
1201 AGAAAACCTTCAATGTCAGACCTTAACTGTTTTCACATAGTCTCTCAAGAAAGACTTATTTTAACATTGTTAAAAACAAA
1281 CTCTTAACCCTCATCCATAATTAAAAGGCAGTACAGTGCTCAAACCTTCTAGCTGCCAATATTATTTTTTTATAATTACA
1361 TTAACTCTTTACATGAAATACTCCCCTGACCACAGCATGTATTTATGACCTTCTGAACAGTATCGTCTTGTGCACAAAAC
1441 CAAAATTACAATCAATGTCATATTTATATCAAGGTCTTACCTTTCAATATCAAAATCCCCTGGAGCCTATTTTTTTAATC
1521 TTAAACTCAATGAGTATATGTTGAGTAACCTTTAAAAATGAAAGATTTAAAAAGATTTATATCCACTGAGAATACAGGCC
1601 TTATATTAAGGATGATATTAAAATTATAGGTTTTATTTGTCATTTTTAACTACCTCTCATATTTTTAATTTATTAAACAT
1681 ATTTTTTTAAAGCACAAGTTTGGAGTTTTCTTTTTAACTTTATTTTACTTGAAAGGTTGGCATCATGTTTCTTTTTCCAT
1761 AAATTTAAAACATTTCAAGAATTGTTTTTTAAGTGAATATGATTTTCTAATTTATATAGAATTCAGGAGCTATGATTCAA
1841 AGGGCTATTACATATTCCCTATGCAAATATTTTAACTGTTGCAGTGTTTGACAAAATGGGATTTTAAAAAAACAATTTGC
1921 AGAGTGGAAAAACAAAACGTTGTGTTTACACTGGCGCCGCTGACTATCCTACCATGCTCAGCACTTTTAAATACATTATT
2001 TATGACAATGTAGTTTAAATGCAAGATATTTATAATATTCATCTTTTAAAAAATGCCTGTGGATTTTTTTTTTTCCTGAA
2081 AGTGTTAGTGTGTTTAAGATACGCTTTGCTTATGTCACCTAGATGTTTACTATTTGAATAGTTTTGGTAATCTGTTGAGA
2161 AAAAAAGATAGTTATGAAGATGTTTACAAAAATATAATCCTATTTTTTCCTAAAGAGTCAAGAAAACGTAAAAGCATCAT
2241 GGCTTCTATTGTGAAGTCCCACCGTTCTGAATGATTTGATTCTTTCCTTGATTAGTGTTGATTATGCTAATAGGATGTAT
2321 ACACATGTATAGTGTACCATCATCCTCGTTCTGTGTTTGGGGCACAATGAGTTGGTGCACATGACCAGGAAAAATGTAAT
2401 ATATTACCACACCCTGCATGCATGATTGTTTTCGATGTAGACTTTATTATATCCCTGTTCCATGGATGCCAGTGACTGAC
2481 TGTCACAGGCGATCTTTACAATACGCTCTGGACTATTGGGCGAGCTGTCTGTGTGACAATGCATTTAATATTTTACCAAA
2561 TGGAAGAGATAAGGCTCCTTGAGAGAGTCCTCATCTCTAACCTAGGCATTTGCAGTGTTTGATTTCAAAGTTGCTGTGTC
2641 TTTGGAACCTGTGTACAAGGAGGTTCCAAAGCTGATTTGCACAGTCCCTGCCATTAGCCATTGCTATGTGAGCAGTTCTA
2721 TGCTAAATTCATATTTCAGGGGGATGGCTTCTTAGTTCACTTGAGAATGTTTCAAGTTTTGAAGAGTGTGTCTGTGGACT
2801 TGTGACTATGCCAGTCTTTAAAATATGGTGTGGTTTCTTCTGTTTAAGTCACAGAGCTATAATAAACAAACAAAAGCCCA
2881 CAACTGGTGTCTGCCACCATTTCAAGAGCACAACACTTGATATAGTCAAGTTTGATTTAATTCCTAGTGGGTGAATCGCT
2961 CCCTGGGGAGACCCTAGCACTTTAAATGAAGCACCTTATATTTACGATTGCCAAGATAACTTTGCATATTGTCAGAATCT
3041 GTTAATGCACACACAAGAGGATAACGCACTTTCTATAGTACCTGTCTATAGGCATATCTATTGTGGAAAGCAAAGGAGAT
3121 TGTGTAGGTGATGTATACAGCGGTGACTCAAAGGCAGATGTCTTCATAGGTGGATCTTACACATAGACATGCTAATGCTG
3201 AAGGGGTGACTCTTCTCTTTCTGACTTATTATGACATCATCCAAAGTTAGCACCGATGCTCTGATGGCATGAAAAGGGCA
3281 TATGAATGTTAACAAAACAGGATTTTGATACTGAAGTTATAAATGTAAAAGTAAAAATTACAATATGGCCTTTAAAACAG
3361 TCTTACAATATTTATATATGACCAGTTCTAACAGTGTGTCACTGCCTAGAACATTTATTAGTGCCCTTCAATGTCTCCTA
3441 GTATACAGAATGTGCTATATTTATTTGATAGTTAAGTGTTAAAGGACTATAGAAATAGATAAAGGCTTATCTGTTGAAAA
3521 CAAATGTACATCTCTTCCCATTATGCTAATTTAAAATACTTGAATCTTAATTTAAGTTCATTCTTTTATTCTAGAATTTT
3601 TTTAATAGTCTCAGAAAAATGTCATATTGTAATTGTGGAAACTACACTTGCAACAAATAAAAAACTTCAGGTGCTTTGGT
3681 GAAGGTTTTTAAGTGGAAGGAATGAAAATAAAGCATAGAAAAATGAACTTTTTGAAGAACCATCAATTTGTTTTTAAACA
3761 GGTAGAAGATACACCTAGAACCCGACATAACATGAATTTTTGAATACTTTCAGCTTTTATATATTGACATATTATTTTGA
3841 AACAGGAAATACGGTCAACAGATTGGGTTACCTATCTATTTAGATTGGCAGTTAATGACCAATCTCCAAAGTATCTATTT
3921 TTAAGTCTGTTTGAGTACTCTTTTCTGTTCTTAAATTTCTGATGATCATAAAATGCTGTCCCTAGAAAGGAGCATACCAT
4001 GTGTAATAATCTTAGCAATTCTCCACTTGCCACAATTGCAAATATTTACCACTAAAACCTACCAAACCTTCCTTCAGAAC
4081 ATGTCTGCAAGATTATAATATCGTCTTAACATCTTTGCAGTGCACACAGTGCATGGAGGAGCACTGGCTTTGATTTCTGT
4161 GGGTGTTAAAAAATATTTCTTTTACAAATAAAGTCCTAAGCAGCCATAAGGGACATGTTTTCTTTCCCACTGATTGAAGA
4241 GACTATACCAGACCATCATTCTGTAGAAAACAGGGAGATGGAGTGTAGCCTTCGTTGGGCAAATGGGGTTGTGGACTCTG
4321 ATTCTCATGACATAAAGCTCTAAGTTGAGAAAAAGCCATGACCTAAAGTATTCGTGATGATAGCCACATGTACTATTTTA
4401 ATGTCGTGCATGAAATCTGAAATTCAGTCCCCTCCTCATGTCTTATATCGTATGTCTTCTGTTGCAAAGTTTTCCATTGA
4481 TTCGTGCCTTTATCACAGCATTTTCAGCTTTTTAAACAGTTAGAAATATGTGAGCTTTAAACCGATGATTAAACAAACTG
4561 TCTAAGATTTGGGCACATTTCCTTTTGAAATGACAGCTGATTTCGAGTTGTATTTCTATCCAGAGGTGGTAGGGAAAGTC
4641 ACGTAACCTTTCTGAATCTGAATCTGTTTCTTTGTCTTAAAATATTGACTATGGGGTGTCCATGGGAACAGTAATGTTGC
4721 ATATAATAATTGACCGGAGTTCTTCAGCCTAGTAACAATAACATTTTTTGGAAGGTCTGTAGTTGGTGTCATTTCACTGT
4801 ATTCTCTCCTCGTGTTTCCTTGGAAAGTTTAGGTTAGCACACAAAGAGGGTTCATCATGACATTTTCATACCAGTGTCTC
4881 ATGATATCTTCTTCTCATTTGTTCCTCACTGCCTTTCCCAGGGCCTTCTACTTTCTTTGCTTATTTTTTTTTAAACATAC
4961 TTGAAACTTTAAAATGTGCATAGGTATGGACAAACACCATACACACACACACACACACACACACATGCATACAGAGACAC
5041 ATATACACACAGACACAGACATACAGTTATGCCGCCCACAGACACATGCACAAGCACACATACACACAGACACATCGAAA
5121 CATCCACACACACCACACACACACATACAGAAATACACACTCAAAGATGGAGACATAGGCAATAAAATGACCATCCCTGT
5201 GGGTGGGAATACTATAGAATCAAGTTAGTTACCAGTATGCCAAGTATGCCAGTGGGCAACAGAGCTGAAAGCAATGTCAT
5281 AAATCATGATTCGAAAATAATCCCTTCAATATTAGTGTGACTTTGTCGATTCTATCATGAAACTATGAATAGAAAGATGA
5361 ATACAAGTGGCTAAGTGTTCATTAACAGTTTCACCAAACTTTTGAATATTTTCAGCTTTATAGCAAAATGATGAGGCAGT
5441 AAATGCCCTCATGTGTTCTATGTGTCTGGGAAGCAATTTTCCCAAATAATAACTAAAATTGACCCATCGAGTCTGTGTTC
5521 CACTCCATCATCATCCTTCACCTCTTGCCTTCTCTGCCCTTCCCATCCTGTAACCACACGACCTCCTGTTGTTTCCTCTC
5601 TTTGTGTTTCTTTCAGCTGTGACTCTCTCTAAATGGTCTTGTTTTTTCAAAACCTTGACAGTTGTGAGTGGAAGTGATGT
5681 CTATTTTACAGAGCATCGCTCTGCAGGGTGGGGTTTGTCAAGACTTCTCCTGTTTATATGAGTGGGGATTTTTTTTTTGT
5761 TTTTGTTTGTTTGTTTTTAGTTTCTGAAAGGAATAATGTTATTCTGTTCTTTCTATCATAAATATTTGTTTTTATTTTAT
5841 GTGATTGAGTGCTTTGTCTGCATGTATGTATGTGACCACATGTGTGCAGCACTTGAGGCCAGAAGAGTCCTCTGGAATTG
5921 GAGCAACAGATGGTCTTAAGCCTCCATATGGGTTCTGGGTACTGAATCAGGGTCTTCGGTTCTCTTAACTGTTGAGCCAC
6001 CTGTCCAGCCCCGAATAGCCAACCTTATTACATGATATCTACTTGCCACTAGGGTAAGTACTCTTGCTTTGGGCTCCTCC
6081 TTCCTATTGCTGTTGCTGTTCGCCCTGTCACCTGATTTAAGCATTGCCTGTTGGGTACAGCCTCTGCTGCCAAGTGATCC
6161 ACATGCAAAGGTGTCTTAGAGGGAACTAGACCCTGCATTTACATTGAGCAAGAAAAACAACCAACCCCACCCCCCATAAA
6241 AACAACTCTGGCCAATTTCTTAGCATGAATACTAGGCTCCATTAAAGGATTACTAATAAATACTATAAAGAAAATGCAAT
6321 GGATTAAGAAGTGAAATAAGTCCGAAGCAGTGTTAGCTTGGCTATCTACCTAATAAAAGTGCAACTGTTCTCTCTTCCCT
6401 GAAGCACCCTGCATACTTATCTAGTTCCTGCACACATGCTGGATTTGAAGATTTTAGCAAGAGGTTATTTATTACTCGTG
6481 TTTAATGCCTTCCTATATACCATCCCCTGAGCAATGTATACAATGCTGAAGTATAGAAAATTTGATGTTGTTCCTCAAGC
6561 TTTAACCCGTTTGTCATAGTGTTTCTTGTGTGTGTTTTTAAAGAGTTTGCAAAATACTAAAAAAAAGTATTTTAATGAAT
6641 TTGATTCTGTGAAATACATCATTTTGAACATGTATAATTTCTTACTTTCATGCTCATTCTATTTAGTGATCTATTTCCAT
6721 ATTTTGTATTCTATCAATATATTTATTAGTGGACCACAAAAAGGTTCCGAGTACATGAATATAAGAGTAGCCCTGCTTGG
6801 CTATTTTATAATACCAAAATACCTGTGGGTACTCATAGTTGAATAGATACTCCAGATTCTTTTCCTTGCAGCTCAAGTTT
6881 GTCTGGGTTTTTCTTCCATTGAACAGATTTCATCATTTCCCTATGTACAATAGTAGACTTCCTGAAAGTGCTACAGTTTT
6961 TACCATGTAATAGACTTGAGCATGACAATACTGTAAGAAAATACAGAAGTCTCTAAATATGTCTGAGTTTTCAAAGGAAA
7041 TCAAATAATAGGTGTTTTCATTTGACTATATTGTGGCATTTATGGGTATTACTGTAAAATGCATATGCATTACCTGATTT
7121 TCTTAACATGTTTTGAACTAATAAAGAATTCTCCCATGTATCCTTAGACAAGTTATTGTTTGCTTATTGGTGTAATGCTT
7201 ATCTGAGTATAACAACTTCTGGGTGGAGTCTTCACATTGTTATGTAACATTAGAGTACCTTTTTATAAGAAATAAAATAT
7281 GTTCTTGAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ggucGAUCGUUAC---GUGACGGa 5'
              ||| ||:||   ||||||| 
Target 5' agttCTAACAGTGTGTCACTGCCt 3'
 3384 - 3407 161.00 -18.70
2 
miRNA  3' ggucGAUCGUUACGUGACGGa 5'
              ||:|: :| ||||||| 
Target 5' ccctCTGGTTGTTCACTGCCc 3'
 620 - 640 157.00 -17.70
3 
miRNA  3' ggucgaucguuacGUGACGGa 5'
                       ||||||| 
Target 5' ctcatttgttcctCACTGCCt 3'
 4894 - 4914 140.00 -12.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0003460
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013581. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ggucgaUCGUUAC---GUGACGga 5'
                | ||:||   ||||||  
Target 5' ------AACAGUGUGUCACUGC-- 3'
 		1 - 16
Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
41 mmu-miR-449c-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577806 Rnmtl1 mitochondrial rRNA methyltransferase 3 1 1
MIRT581641 Prex2 phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 1 2
MIRT587396 Dnase2a deoxyribonuclease II alpha 1 1
MIRT588268 Smco1 single-pass membrane protein with coiled-coil domains 1 1 2
MIRT595436 9930013L23Rik cell migration inducing protein, hyaluronan binding 1 1
MIRT595972 Tbc1d2 TBC1 domain family, member 2 1 1
MIRT596032 Vps9d1 VPS9 domain containing 1 1 1
MIRT596042 Ubp1 upstream binding protein 1 1 1
MIRT596048 Shisa7 shisa family member 7 1 1
MIRT596070 Ywhag tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 1 1
MIRT596093 St6galnac3 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 3 1 1
MIRT596128 Rgs17 regulator of G-protein signaling 17 1 1
MIRT596176 Med22 mediator complex subunit 22 1 1
MIRT596199 Fyco1 FYVE and coiled-coil domain containing 1 1 1
MIRT597335 Sema4b sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B 1 1
MIRT598319 Mbd4 methyl-CpG binding domain protein 4 1 1
MIRT599424 Cercam cerebral endothelial cell adhesion molecule 1 1
MIRT599598 Bloc1s3 biogenesis of lysosomal organelles complex-1, subunit 3 1 1
MIRT599659 B3galtl beta-3-glucosyltransferase 1 1
MIRT600185 Wipf2 WAS/WASL interacting protein family, member 2 1 1
MIRT600239 Trim36 tripartite motif-containing 36 1 1
MIRT600385 Slc35e2 solute carrier family 35, member E2 1 1
MIRT600677 Mapre1 microtubule-associated protein, RP/EB family, member 1 1 1
MIRT601685 Sco1 SCO1 cytochrome c oxidase assembly protein 1 1
MIRT601718 Rheb Ras homolog enriched in brain 1 1
MIRT601800 Rab11b RAB11B, member RAS oncogene family 1 1
MIRT601868 Pin1 protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 1 1
MIRT602435 Epb4.2 erythrocyte membrane protein band 4.2 1 1
MIRT602459 Dusp11 dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) 1 1
MIRT602620 Cenpl centromere protein L 1 1
MIRT602637 Cd93 CD93 antigen 1 1
MIRT602799 Ankrd28 ankyrin repeat domain 28 1 1
MIRT603072 Zfp446 zinc finger protein 446 1 1
MIRT603468 Trmt10a tRNA methyltransferase 10A 1 1
MIRT604685 Sgk3 serum/glucocorticoid regulated kinase 3 1 1
MIRT605120 Cnnm3 cyclin M3 1 1
MIRT605335 Zfp317 zinc finger protein 317 1 1
MIRT606441 Fam118a family with sequence similarity 118, member A 1 1
MIRT606475 Ctsa cathepsin A 1 1
MIRT606636 Phc3 polyhomeotic-like 3 (Drosophila) 1 1
MIRT736224 TUBB4B tubulin beta 4B class IVb 3 0

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