pre-miRNA Information
pre-miRNA mmu-mir-466h   
Genomic Coordinates chr2: 10514891 - 10514971
Description Mus musculus miR-466h stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-466h-5p
Sequence 11| UGUGUGCAUGUGCUUGUGUGUA |32
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Slc6a8   
Synonyms AA589632, CRT, CRTR, CT1, CTR5, Creat
Description solute carrier family 6 (neurotransmitter transporter, creatine), member 8
Transcript NM_001142809   
Other Transcripts NM_001142810 , NM_133987   
Expression
Putative miRNA Targets on Slc6a8
3'UTR of Slc6a8
(miRNA target sites are highlighted)
>Slc6a8|NM_001142809|3'UTR
   1 CAGGCACCCCCAGCTCACATCACCAGCTCATCCTTTGGTAGCCATAGCAGCCCCAACTTTAGCCCCCACTCATCCCTCCG
  81 GGGGCTTGCCTTTCCTTGACAGTTTGGGGGTCTGCCTGGGGGAGGGGAGGGAGCACCATGAGTGCTAACAAAAATAACTT
 161 TTTCCATTTTTAATAAAACGCCAAAAATATCACAACCCACCAAAAATAGATGCCTCTCCTCCCCACCCCACAACTAAGCT
 241 GGTATTTGCATTGCTTCCAGGCCCTACCTCCTCTCCTTCTTTCCAGTGCCCAGGACCCATCTCCTAGCCTTGCCAAGCCC
 321 ACCTGCCTGCCTCCCAAGCTCTGCTCTGTAACATACCTTTGCCCTAGAAACAACAGTGGCAGCTTGGGTAACAGGAGAGG
 401 AGGGAAAATAAGGTAGGACAGGAGGAGAAGAGAGGGACAGTGGAACCAATGTGAATATTGCAGCTGAATTAGACCCTTCC
 481 TACCCCTATTCTAGAAGCTAGAGAGCCAGTCAGCAATGGAACTTTGTGTTCCTGCGCCAATTGCCACCAATATTAACTGT
 561 GTGAGCTTGGGTCTGAGTGCACACCCCCCTCTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGTGTGTATGTGTAGGTCT
 641 GTAGATCTCTTATCCCTTAGTGAAGGTGAATACCAGAAGTAAACACAGCCTCTGGGCAAAGGAGGCTTATATTTTGCACA
 721 TTTTATAAGAAAAACTTGAGAGTGAGACTTCTGCTTGTATATTTCTAAAAAGCCCCCACCATCTCCACATTTCCACTCCT
 801 TTCCTTCACATCCTCACCTTTCTTCTCTGACCCTAGCCAAGGAGTGTGAATTTATAGACTTAATTTTCATAGGCAAAACA
 881 AAAGCTTCAGGCTGTTGAGTATATATATGAGTCTGTTGTATGGATGTTCTGTATGGTCTCCAGCCAGAGACTGGAGTGGG
 961 AAAGTGGGAGCCTCAATGGTGTCCCCACACTGCTCCTTTGCCTCTAAGTCTGTGGGACGGAAGGCTGTAACACTGTTCCA
1041 GGGAATCTGGGTCTGCTCACCTGACATGCTCAGACTTGCCCACACCCAGCTCCCTGGGCAGGATTGCAGGGATTTCCCAT
1121 GGACAAAGCCCGGGAAAGGGATCCTGGGCATGGCTCCCACCTCCAAGCTAAAAGGCTGATGTACTTTCGACATCTCAAGT
1201 CTTCCGTGTAGCAGCTTAAACCCACATCTGTGTCCCATCAAGCCTGAAACAGCCAAGTAGCCCTTAGAAAGGCTTCCTTC
1281 ACACAGAGCTGGACTAAGCCAGAGAGGTGGGGCTGGGTAAGGGTGCAAGCCTTTGGGGACATTCTTACTGTGCTAAAAAA
1361 GCCACTGCAAACATAGCAATAAAAACACTTCATTTTCCAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' auguguguucgugUACGUGUGu 5'
                       :||||||| 
Target 5' agcttgggtctgaGTGCACACc 3'
564 - 585 141.00 -10.80
2
miRNA  3' auGUGUGUUC---GUGU---ACGUGUGu 5'
            || | :||   :|:|   ||||||: 
Target 5' ggCAAAGGAGGCTTATATTTTGCACATt 3'
695 - 722 132.00 -9.30
3
miRNA  3' auGUGUGUUCGUGU-ACGUGUGu 5'
            :| | |||| || |||| || 
Target 5' gcTAAAAAAGC-CACTGCAAACa 3'
1352 - 1373 127.00 -9.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions CD4+ T cells (C57BL/6)
Disease MIMAT0004884
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... "HITS-CLIP data was present in GSM1013579. RNA binding protein: AGO2. Condition:CD4+ T cells "HITS-CLIP data was present in GSM1013580. RNA binding protein: AGO2. Condition:CD4+ T cells ...

- Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' auGUGUGUUCGUGUACGUGUGu 5'
            :::::: |::::||::::: 
Target 5' ucUGUGUGUGUGUGUGUGUGUg 3'
7 - 28
Article - Loeb GB; Khan AA; Canner D; Hiatt JB; et al.
- Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
CLIP-seq Support 1 for dataset GSM1013579
Method / RBP HITS-CLIP / AGO2
Cell line / Condition CD4+ T cells (C57BL/6) / CD4+ T cells, 155KO, biological rep5
Location of target site NM_001142809 | 3UTR | CCCCCCUCUGUGUGUGUGUGUGUGUGUGUGUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23142080 / GSE41285
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM1013580
Method / RBP HITS-CLIP / AGO2
Cell line / Condition CD4+ T cells (C57BL/6) / CD4+ T cells, 155KO, biological rep6
Location of target site NM_001142809 | 3UTR | CCCCCCUCUGUGUGUGUGUGUGUGUGUGUGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23142080 / GSE41285
CLIP-seq Viewer Link
87 mmu-miR-466h-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577339 Zfp157 zinc finger protein 157 2 2
MIRT577488 Tns4 tensin 4 2 6
MIRT578067 Oxsm 3-oxoacyl-ACP synthase, mitochondrial 2 2
MIRT578343 Ltf lactotransferrin 2 2
MIRT578743 Gm4841 predicted gene 4841 2 4
MIRT578822 Fermt1 fermitin family member 1 2 2
MIRT579127 Chrnd cholinergic receptor, nicotinic, delta polypeptide 2 2
MIRT579135 Chrna1 cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) 2 2
MIRT579305 BC021785 major facilitator superfamily domain containing 4B5 2 4
MIRT579417 Agtrap angiotensin II, type I receptor-associated protein 2 2
MIRT579732 Zfp92 zinc finger protein 92 2 4
MIRT579978 Wrn Werner syndrome RecQ like helicase 2 4
MIRT581035 Sim1 single-minded homolog 1 (Drosophila) 2 4
MIRT581532 Pten phosphatase and tensin homolog 2 2
MIRT582312 Nab1 Ngfi-A binding protein 1 2 4
MIRT582843 Itga9 integrin alpha 9 2 4
MIRT582976 Igf2 insulin-like growth factor 2 2 4
MIRT583470 Foxk1 forkhead box K1 2 4
MIRT584662 B4galt6 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 2 4
MIRT585220 Zfp488 zinc finger protein 488 2 4
MIRT585299 Zfp26 zinc finger protein 26 2 2
MIRT585311 Zfp248 zinc finger protein 248 2 4
MIRT585835 Slc6a17 solute carrier family 6 (neurotransmitter transporter), member 17 2 4
MIRT586077 Rhobtb1 Rho-related BTB domain containing 1 2 2
MIRT586329 Pgm5 phosphoglucomutase 5 2 4
MIRT587381 Dzip3 DAZ interacting protein 3, zinc finger 2 2
MIRT587611 Cml2 N-acetyltransferase 8 (GCN5-related) family member 2 2 2
MIRT587631 Clec7a C-type lectin domain family 7, member a 2 8
MIRT587725 Cd28 CD28 antigen 2 2
MIRT587758 Cd200r1 CD200 receptor 1 2 2
MIRT588012 Akap7 A kinase (PRKA) anchor protein 7 2 2
MIRT588296 1700019G17Rik N-acetyltransferase 8 (GCN5-related) family member 4 2 2
MIRT588555 Uhrf1bp1l UHRF1 (ICBP90) binding protein 1-like 2 2
MIRT588689 Tet2 tet methylcytosine dioxygenase 2 2 2
MIRT588893 Slc1a2 solute carrier family 1 (glial high affinity glutamate transporter), member 2 2 4
MIRT589021 Rnf11 ring finger protein 11 2 2
MIRT589101 Rasal2 RAS protein activator like 2 2 4
MIRT590106 Etv3 ets variant 3 2 2
MIRT590170 Elovl6 ELOVL family member 6, elongation of long chain fatty acids (yeast) 2 4
MIRT590929 Tacr2 tachykinin receptor 2 2 2
MIRT591061 Ptpre protein tyrosine phosphatase, receptor type, E 2 4
MIRT591144 Nsun3 NOL1/NOP2/Sun domain family member 3 2 2
MIRT591210 Mdm2 transformed mouse 3T3 cell double minute 2 2 2
MIRT591429 Car10 carbonic anhydrase 10 2 4
MIRT591502 Acot2 acyl-CoA thioesterase 2 2 4
MIRT591557 Zfp449 zinc finger protein 449 2 2
MIRT591632 Ubtf upstream binding transcription factor, RNA polymerase I 2 4
MIRT591952 Ceacam1 carcinoembryonic antigen-related cell adhesion molecule 1 2 2
MIRT591973 Ap1ar adaptor-related protein complex 1 associated regulatory protein 2 2
MIRT592040 Tnfrsf13c tumor necrosis factor receptor superfamily, member 13c 2 2
MIRT592091 Smo smoothened, frizzled class receptor 2 2
MIRT592109 Slc25a12 solute carrier family 25 (mitochondrial carrier, Aralar), member 12 2 2
MIRT592174 Oxtr oxytocin receptor 2 2
MIRT592217 Map3k7 mitogen-activated protein kinase kinase kinase 7 2 2
MIRT592224 Magee2 melanoma antigen, family E, 2 2 6
MIRT592250 Lcp2 lymphocyte cytosolic protein 2 2 4
MIRT592267 Kcnj16 potassium inwardly-rectifying channel, subfamily J, member 16 2 2
MIRT592390 Trp53i11 transformation related protein 53 inducible protein 11 2 6
MIRT592420 Stxbp5l syntaxin binding protein 5-like 2 2
MIRT592441 Snx12 sorting nexin 12 2 2
MIRT592515 Npr3 natriuretic peptide receptor 3 2 2
MIRT592626 Mbnl3 muscleblind like splicing factor 3 2 4
MIRT592703 Gfra2 glial cell line derived neurotrophic factor family receptor alpha 2 2 2
MIRT592746 Epas1 endothelial PAS domain protein 1 2 2
MIRT592765 Dmd dystrophin, muscular dystrophy 2 4
MIRT592843 Akap2 A kinase (PRKA) anchor protein 2 2 2
MIRT592989 Cacna2d2 calcium channel, voltage-dependent, alpha 2/delta subunit 2 2 2
MIRT593003 Bend4 BEN domain containing 4 2 2
MIRT593228 Fndc3a fibronectin type III domain containing 3A 2 2
MIRT593415 Neurod2 neurogenic differentiation 2 2 4
MIRT593453 Rab11fip1 RAB11 family interacting protein 1 (class I) 2 2
MIRT593482 Havcr2 hepatitis A virus cellular receptor 2 2 2
MIRT593517 Csf2ra colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) 2 2
MIRT594037 2810006K23Rik RIKEN cDNA 2810006K23 gene 2 2
MIRT594129 Fam104a family with sequence similarity 104, member A 1 1
MIRT595670 Rxrb retinoid X receptor beta 2 2
MIRT595701 Fndc7 fibronectin type III domain containing 7 2 2
MIRT596268 Bnc2 basonuclin 2 2 2
MIRT596281 Slc6a8 solute carrier family 6 (neurotransmitter transporter, creatine), member 8 2 2
MIRT598763 Gpr68 G protein-coupled receptor 68 2 2
MIRT601111 Btrc beta-transducin repeat containing protein 2 2
MIRT601202 Arhgef9 CDC42 guanine nucleotide exchange factor (GEF) 9 2 2
MIRT603151 Ubxn8 UBX domain protein 8 2 2
MIRT603192 Trim65 tripartite motif-containing 65 2 2
MIRT604581 Trim71 tripartite motif-containing 71 2 2
MIRT605447 St18 suppression of tumorigenicity 18 2 2
MIRT605591 Ncam1 neural cell adhesion molecule 1 2 2

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