pre-miRNA Information | |
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pre-miRNA | mmu-mir-30a |
Genomic Coordinates | chr1: 23272269 - 23272339 |
Synonyms | Mirn30a, mmu-mir-30a, Mir30a |
Description | Mus musculus miR-30a stem-loop |
Comment | Landgraf et al. confirm that the 5' mature miRNA product is the predominant one . |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
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Mature miRNA | mmu-miR-30a-5p |
Sequence | 6| UGUAAACAUCCUCGACUGGAAG |27 |
Evidence | Experimental |
Experiments | Cloned |
Putative Targets |
Biomarker Information |
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Gene Information | |||||||||||||||||||||
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Gene Symbol | Ing1 | ||||||||||||||||||||
Synonyms | 2610028J21Rik, AA407184, AI875420, mING1h, p33Ing1, p37Ing1b | ||||||||||||||||||||
Description | inhibitor of growth family, member 1 | ||||||||||||||||||||
Transcript | NM_011919 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Ing1 | |||||||||||||||||||||
3'UTR of Ing1 (miRNA target sites are highlighted) |
>Ing1|NM_011919|3'UTR 1 TGAGTGGACACTCACCCGTGGTCAGTGACACAGCCACCAGTGTGTTTATGGTATCGCTGCCTTCGTGGAAGTCCGAGGGC 81 AGTCAGATGAGTATTTTAGAGAATGTTAGCCGTGCCTCCTCTCCTCGGATGGCGGAGACAGCCTGCGTCTTCATGGTGTA 161 CCTTGTTCCCAGACTGGGTCTGCAGAGCCGCAATTTAGAAACTACAAATACAGGTTCGAATTAACCATGTCAGCGAGTGT 241 CAGACTGATTTTGGGGGTTGGGGAGTGACTTGGAAGTAAGCTAACAGCTGTATAAAGAAGAGATTTCATTTGGCTGTTTT 321 GACAAAAATGTTACGGTTTACAAAAAAAAAAAAAAATTAGCCAGGTTGCTGAAGTACAACAAGCGTGCTTCTTGCTACCA 401 TAATGTATATCCACACGACAAGTTGGTGGTCTAAAGTCTAAATATTATTATTTTTTAAAGAGGTAAATGGGTAAATTTTA 481 CATGACATATTTTATACATGGCCTATTCCCTAACTGGCCATTTTTAATGACTGGGTACATTTTTAATAGGTCAGACACAA 561 GTGGTCCAGGCAGTGGGTCCGAGTCTTGCCTTTGCTACCCTAGGTCATGGTGTAGCCACCTTTAACTTATATGAAGTGTA 641 TAAATGTACATCTTGCCCCTGCTGTATCATAACCGGAAGTGCAGCCTGGTGCTGTTATGAAGCCAAGGTGTGCATCCTGC 721 TGCGTGTGTGAGCTGTATAGATGTTGCGTCAAGAAATAAATGAAACTTGGCCAGTTTGTTTCTTTAGCATTTAATTTTAA 801 CATACAAATAATTTTAAATTTTTGTCTTAAAAATTTATACACCAGCAGTTTAGACAAAGCCTTAAGCAAATTGTATATTA 881 TTGATCTCACGTAATAAGGAAGTAGGCATTATGTCCATGCCAGCAAATACATGTCAAAGGTGTTTAGATCAGAGGTTAGG 961 ACGTGACTGTGCTTGTGAGGTCCAGCCCCACCATCTGTTTTTGTATGGCCTGTGAGCTAAGAATGGTCTCTATGTTTCTG 1041 AGTGGTTTTTAAAATCACATTTCATGATACACGAAAGTCCTATGAAATGAAGATTTGCGTGTCTATAAATAAAGACTCAT 1121 TGGAACACCAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | CD4+ T cells (C57BL/6) |
Disease | MIMAT0000128 |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
"HITS-CLIP data was present in GSM1013576. RNA binding protein: AGO2. Condition:CD4+ T cells
... - Loeb GB; Khan AA; Canner D; Hiatt JB; et al., 2012, Molecular cell. |
Article |
- Loeb GB; Khan AA; Canner D; Hiatt JB; et al. - Molecular cell, 2012
MicroRNAs (miRNAs) are essential components of gene regulation, but identification of miRNA targets remains a major challenge. Most target prediction and discovery relies on perfect complementarity of the miRNA seed to the 3' untranslated region (UTR). However, it is unclear to what extent miRNAs target sites without seed matches. Here, we performed a transcriptome-wide identification of the endogenous targets of a single miRNA-miR-155-in a genetically controlled manner. We found that approximately 40% of miR-155-dependent Argonaute binding occurs at sites without perfect seed matches. The majority of these noncanonical sites feature extensive complementarity to the miRNA seed with one mismatch. These noncanonical sites confer regulation of gene expression, albeit less potently than canonical sites. Thus, noncanonical miRNA binding sites are widespread, often contain seed-like motifs, and can regulate gene expression, generating a continuum of targeting and regulation.
LinkOut: [PMID: 23142080]
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57 mmu-miR-30a-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT003714 | Ak1 | adenylate kinase 1 | ![]() |
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4 | 1 | ||||
MIRT003715 | Tnrc6a | trinucleotide repeat containing 6a | ![]() |
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4 | 1 | ||||
MIRT003716 | Abcb1b | ATP-binding cassette, sub-family B (MDR/TAP), member 1B | ![]() |
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3 | 1 | |||||
MIRT003717 | Inhba | inhibin beta-A | ![]() |
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3 | 1 | |||||
MIRT003718 | Egfr | epidermal growth factor receptor | ![]() |
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4 | 2 | ||||
MIRT004770 | Bdnf | brain derived neurotrophic factor | ![]() |
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5 | 2 | |||
MIRT007045 | Snai1 | snail family zinc finger 1 | ![]() |
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2 | 1 | ||||||
MIRT054524 | Ccne2 | cyclin E2 | ![]() |
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2 | 1 | ||||||
MIRT054525 | Celsr3 | cadherin, EGF LAG seven-pass G-type receptor 3 | ![]() |
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2 | 1 | ||||||
MIRT054526 | Mdm2 | transformed mouse 3T3 cell double minute 2 | ![]() |
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2 | 1 | ||||||
MIRT054527 | Mtdh | metadherin | ![]() |
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3 | 2 | |||||
MIRT054528 | Smad1 | SMAD family member 1 | ![]() |
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2 | 1 | ||||||
MIRT054529 | Twf1 | twinfilin actin binding protein 1 | ![]() |
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2 | 1 | ||||||
MIRT054530 | Timp3 | tissue inhibitor of metalloproteinase 3 | ![]() |
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2 | 1 | ||||||
MIRT577999 | Pgr | progesterone receptor | ![]() |
1 | 1 | |||||||
MIRT578189 | Narg2 | interactor of little elongation complex ELL subunit 2 | ![]() |
1 | 1 | |||||||
MIRT578280 | Mesdc2 | mesoderm development LRP chaperone | ![]() |
1 | 1 | |||||||
MIRT578462 | Ints12 | integrator complex subunit 12 | ![]() |
1 | 1 | |||||||
MIRT578487 | Il18r1 | interleukin 18 receptor 1 | ![]() |
1 | 2 | |||||||
MIRT580036 | Wdr89 | WD repeat domain 89 | ![]() |
1 | 3 | |||||||
MIRT580042 | Wdr44 | WD repeat domain 44 | ![]() |
1 | 1 | |||||||
MIRT580299 | Tomm70a | translocase of outer mitochondrial membrane 70 homolog A (yeast) | ![]() |
1 | 1 | |||||||
MIRT581001 | Six4 | sine oculis-related homeobox 4 | ![]() |
1 | 1 | |||||||
MIRT581268 | Rrad | Ras-related associated with diabetes | ![]() |
1 | 1 | |||||||
MIRT582213 | Nfatc3 | nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 | ![]() |
1 | 1 | |||||||
MIRT582448 | Mgea5 | meningioma expressed antigen 5 (hyaluronidase) | ![]() |
1 | 1 | |||||||
MIRT583057 | Hoxb3 | homeobox B3 | ![]() |
1 | 1 | |||||||
MIRT583627 | Strip1 | striatin interacting protein 1 | ![]() |
1 | 1 | |||||||
MIRT583684 | Amer1 | APC membrane recruitment 1 | ![]() |
1 | 1 | |||||||
MIRT583926 | Dpy19l1 | dpy-19-like 1 (C. elegans) | ![]() |
1 | 1 | |||||||
MIRT584694 | Cep170b | centrosomal protein 170B | ![]() |
1 | 3 | |||||||
MIRT585022 | Gskip | GSK3B interacting protein | ![]() |
1 | 1 | |||||||
MIRT585237 | Zfp446 | zinc finger protein 446 | ![]() |
1 | 1 | |||||||
MIRT586099 | Reep4 | receptor accessory protein 4 | ![]() |
1 | 2 | |||||||
MIRT587711 | Cd300a | CD300A molecule | ![]() |
1 | 2 | |||||||
MIRT588338 | Zfp711 | zinc finger protein 711 | ![]() |
1 | 1 | |||||||
MIRT588632 | Tnrc6b | trinucleotide repeat containing 6b | ![]() |
1 | 3 | |||||||
MIRT589298 | Pigh | phosphatidylinositol glycan anchor biosynthesis, class H | ![]() |
1 | 1 | |||||||
MIRT590161 | Eno2 | enolase 2, gamma neuronal | ![]() |
1 | 1 | |||||||
MIRT592638 | Lpar3 | lysophosphatidic acid receptor 3 | ![]() |
1 | 2 | |||||||
MIRT593645 | Tbc1d5 | TBC1 domain family, member 5 | ![]() |
1 | 1 | |||||||
MIRT595042 | Camk4 | calcium/calmodulin-dependent protein kinase IV | ![]() |
1 | 1 | |||||||
MIRT595217 | Slc16a1 | solute carrier family 16 (monocarboxylic acid transporters), member 1 | ![]() |
1 | 1 | |||||||
MIRT595451 | Ptrhd1 | peptidyl-tRNA hydrolase domain containing 1 | ![]() |
1 | 1 | |||||||
MIRT595826 | Fam227a | family with sequence similarity 227, member A | ![]() |
1 | 1 | |||||||
MIRT596025 | Cdk13 | cyclin-dependent kinase 13 | ![]() |
1 | 1 | |||||||
MIRT596356 | Serpine1 | serine (or cysteine) peptidase inhibitor, clade E, member 1 | ![]() |
1 | 1 | |||||||
MIRT596419 | Ing1 | inhibitor of growth family, member 1 | ![]() |
1 | 1 | |||||||
MIRT596513 | Zfp9 | zinc finger protein 9 | ![]() |
1 | 1 | |||||||
MIRT599308 | Csf1 | colony stimulating factor 1 (macrophage) | ![]() |
1 | 1 | |||||||
MIRT603175 | Trp53rk | transformation related protein 53 regulating kinase B | ![]() |
1 | 1 | |||||||
MIRT731329 | IL21R | interleukin 21 receptor | ![]() |
1 | 1 | |||||||
MIRT733494 | Gfra1 | glial cell line derived neurotrophic factor family receptor alpha 1 | ![]() |
1 | 0 | |||||||
MIRT735519 | SOCS1 | suppressor of cytokine signaling 1 | ![]() |
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2 | 0 | ||||||
MIRT755305 | Runx2 | runt related transcription factor 2 | 2 | 1 | ||||||||
MIRT755548 | SOCS3 | suppressor of cytokine signaling 3 | 3 | 1 | ||||||||
MIRT755880 | NCAM1 | neural cell adhesion molecule 1 | 1 | 1 |
miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||
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