miRTarBase - #MIRT596495


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pre-miRNA Information
pre-miRNA mmu-mir-674   
Genomic Coordinates chr2: 117185127 - 117185226
Synonyms Mirn674, mmu-mir-674, Mir674
Description Mus musculus miR-674 stem-loop
Comment Landgraf et al. show that the 5' miRNA product is the predominant one .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-674-5p
Sequence 25| GCACUGAGAUGGGAGUGGUGUA |46
Evidence Experimental
Experiments MPSS
Putative Targets

Gene Information
Gene Symbol Zfp935   
Synonyms 8430426H19Rik, Es113
Description zinc finger protein 935
Transcript NM_001136496   
Other Transcripts NM_178875   
Expression
Putative miRNA Targets on Zfp935
3'UTR of Zfp935
(miRNA target sites are highlighted)		 
>Zfp935|NM_001136496|3'UTR
   1 CACCCAGTCATCTTAAAAGGCATGAAAGAATTCATACTGGAGAGAAACCCTACAAATGTAATCAGTGTGATAAAGCCTTT
  81 TCCCAATACGTTCATCTCCAAATTCATAGAAGCACACATACTGGAGAGAAACCCTTCAAATGTGATGAATGTGATAAAGC
 161 CTTTTCAAAACACTTTCACCTCCAAAATCATAAAAGAACACATACTGGAGAGAAGCCTTACAAATGTAATCAATGTAACA
 241 AAGCCTTTTCACAACACACTAATCTTCACATTCATAGAAGAACACATACAGGAGAGAAACCCTTCAAATGTAATGAATGT
 321 GATAAAGTCTTTTCCCAATTTGGTCATCTCCAAATTCATATAAAAACACATACTGGAGATAAACCCTACAAATGTAATCA
 401 ATGTAATAAAGCCTTCTACCAGAAACGTAGTCTCCAAACACATATAAGAATACATACTGGAGAAAAACCCTACAAATGTA
 481 ATCAATGTGATAAAGCCTTTTCCCAATACGGTCATCTCTATATTCATAGAAGAATACATACTGGATAGAAACCCTTCAAA
 561 TGTATTGAATGTGATAAACCATTTTCAAAACAATTTTGCTTCCAAAATCATAAAAGAACACATACTGGAGAGAAGCCATA
 641 CAAATGTAATCAATGTGACAGCCTTTTCACAACACAGTTATCTTCAAATTCATAGAAGAAGACATACAGGAGAGAAACCC
 721 TTCAAATGTAATCAATGTGATAAAGCCTTTTCCCAACACAGTAATCTCCACATTCATAGAAGAACACATACGGGAGAGAA
 801 GCCTTACAAATGTACTCAAAGTGACAAAGCCTTTCACAACACAGTTATCTTCAAATTCATAGAAGAAGACATACAGGAGT
 881 TAAACCCTACAAATGGAATCAGTGTGACAAAGCCTTTTCCCAACCTTGTAACCTCCAATCCGTTGAAGAATTTATACTGG
 961 AGTGAAATCTTGCAAATGAAATGAATGTGACAAAGCCTTTCCCCATCATTATAATCTACAAATTCATAAAGGACATACTA
1041 GAGAGAAACCTTACAAGTGTAATCAGTGTGTGAAAGTCTTCAAAACATGGTCATCTTCAAAAGAATAAAAGAATACATAC
1121 TGGAGGGAAAAGCTACGAAGGAAATGGATGTGAGCAACCTTTTTACAACACCATCATTTCTGAATGTATAAGTGAACATA
1201 CTGAAGAGAAAGACTATGAATGTGATCATAGTCTAAATCGTCATGAACACATTTGTAGGGGCCAGAATCCTGAGTATAAG
1281 CAATGTGGAAAAATCTATGCTGTGCAAAGTTATCTCCTAATGCAGGAAGGAGCACATACAGGAGAGAGAGAGAGAGACAC
1361 AGAGAGAGAGAGAATGAATAAATATACACAATGTGGTAAACCTTTGCATATCACAGTTATCTTTGAATACATAAAAGAGC
1441 ACATAATATATCTCAGAATACTTAGAAGAAGACACTGGAGAGAGAAACTCTGTATGAGTAATGTGGTAAGGCCTCTGCAG
1521 TTGACATTTATCTCATAATGTATAAATTAACACAGACATAGCAGGGCGTGGTGGTGCACTCCTTTAAACCCAGCACTCGG
1601 GATGCAGAGGCAGGCGGATTTCTGAGTTCGAGGCCAGCCTGGTCTAAAAAGTGAGTTCAAAAAAAAAGGGGGGGGGGCTG
1681 GTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCTGGAGATCAAATCCCAGCAACCACATGGTGGC
1761 TCACAACCATCTGTAACTAGATCTGACGCCCTCTTCTGGAGTGTCTGAAGGCAGCTACAGTGTACTTACATATAATAAAT
1841 AAATAATAATAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' augUGGUGAGGGUA--GAGUCACg 5'
             :|:: |   ||  ||||||| 
Target 5' gggGCTGGTGAGATGGCTCAGTGg 3'
 1674 - 1697 141.00 -10.50
2 
miRNA  3' auguggugaggguagAGUCACg 5'
                         |||||| 
Target 5' accctacaaatgtaaTCAGTGt 3'
 47 - 68 120.00 -10.90
3 
miRNA  3' auguggugaggguagAGUCACg 5'
                         |||||| 
Target 5' accctacaaatggaaTCAGTGt 3'
 884 - 905 120.00 -10.90
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1 HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
94 mmu-miR-674-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577119 Prps2 phosphoribosyl pyrophosphate synthetase 2 1 3
MIRT579212 Ccdc25 coiled-coil domain containing 25 1 4
MIRT580735 Srp72 signal recognition particle 72 1 2
MIRT581673 Ppp2r2c protein phosphatase 2, regulatory subunit B, gamma 1 1
MIRT582930 Il15ra interleukin 15 receptor, alpha chain 1 1
MIRT584530 Bzrap1 TSPO associated protein 1 1 1
MIRT584648 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 1 1
MIRT586666 Mbp myelin basic protein 1 2
MIRT588993 Rpl7l1 ribosomal protein L7-like 1 1 1
MIRT589588 Magt1 magnesium transporter 1 1 1
MIRT590893 Tmem69 transmembrane protein 69 1 2
MIRT590965 Slfn5 schlafen 5 1 2
MIRT591089 Pola2 polymerase (DNA directed), alpha 2 1 2
MIRT591253 Loxl2 lysyl oxidase-like 2 1 2
MIRT591405 Churc1 churchill domain containing 1 1 2
MIRT591425 Casc4 cancer susceptibility candidate 4 1 2
MIRT591686 Slc23a1 solute carrier family 23 (nucleobase transporters), member 1 1 2
MIRT591985 A230046K03Rik WASH complex subunit 4 1 2
MIRT593044 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 1 2
MIRT593330 Ddx3x DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked 1 1
MIRT594701 Anks1b ankyrin repeat and sterile alpha motif domain containing 1B 1 1
MIRT595566 Sh3rf3 SH3 domain containing ring finger 3 1 1
MIRT595693 Pcsk6 proprotein convertase subtilisin/kexin type 6 1 1
MIRT595863 Myh10 myosin, heavy polypeptide 10, non-muscle 1 1
MIRT596037 Ubp1 upstream binding protein 1 1 1
MIRT596075 Ywhag tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 1 1
MIRT596182 Med22 mediator complex subunit 22 1 1
MIRT596482 Zyg11b zyg-ll family member B, cell cycle regulator 1 1
MIRT596495 Zfp935 zinc finger protein 935 1 1
MIRT596811 Tyk2 tyrosine kinase 2 1 1
MIRT596873 Tspan15 tetraspanin 15 1 1
MIRT597076 Tbc1d24 TBC1 domain family, member 24 1 1
MIRT597180 Spc24 SPC24, NDC80 kinetochore complex component, homolog (S. cerevisiae) 1 1
MIRT597235 Slc7a14 solute carrier family 7 (cationic amino acid transporter, y+ system), member 14 1 1
MIRT597281 Slc22a15 solute carrier family 22 (organic anion/cation transporter), member 15 1 1
MIRT597347 Scd3 stearoyl-coenzyme A desaturase 3 1 1
MIRT597359 Sap18 Sin3-associated polypeptide 18 1 1
MIRT597446 Rin1 Ras and Rab interactor 1 1 1
MIRT597514 Rbbp4 retinoblastoma binding protein 4, chromatin remodeling factor 1 1
MIRT597557 Rab33b RAB33B, member RAS oncogene family 1 1
MIRT597808 Pla2g4b phospholipase A2, group IVB (cytosolic) 1 1
MIRT597974 Nup133 nucleoporin 133 1 1
MIRT598205 Mpv17l Mpv17 transgene, kidney disease mutant-like 1 1
MIRT598257 Mfsd2b major facilitator superfamily domain containing 2B 1 1
MIRT598574 Irak1 interleukin-1 receptor-associated kinase 1 1 1
MIRT598860 Gm10778 predicted gene 10778 1 1
MIRT599029 Fam177a family with sequence similarity 177, member A 1 1
MIRT599181 Dennd5b DENN/MADD domain containing 5B 1 1
MIRT599224 Dcaf4 DDB1 and CUL4 associated factor 4 1 1
MIRT599261 D3Ertd254e DNA segment, Chr 3, ERATO Doi 254, expressed 1 1
MIRT599278 Cyp2e1 cytochrome P450, family 2, subfamily e, polypeptide 1 1 1
MIRT599286 Cxcr2 chemokine (C-X-C motif) receptor 2 1 1
MIRT599294 Cx3cr1 chemokine (C-X3-C motif) receptor 1 1 1
MIRT599328 Comt catechol-O-methyltransferase 1 1
MIRT599355 Commd7 COMM domain containing 7 1 1
MIRT599488 Ccr9 chemokine (C-C motif) receptor 9 1 1
MIRT599583 Brip1 BRCA1 interacting protein C-terminal helicase 1 1 1
MIRT599647 Banp BTG3 associated nuclear protein 1 1
MIRT599683 Arl16 ADP-ribosylation factor-like 16 1 1
MIRT599726 Ankfy1 ankyrin repeat and FYVE domain containing 1 1 1
MIRT600098 1700047I17Rik2 RIKEN cDNA 1700047I17 gene 2 1 1
MIRT601167 Atrn attractin 1 1
MIRT601187 Asxl2 additional sex combs like 2 (Drosophila) 1 1
MIRT601347 Zfp202 zinc finger protein 202 1 1
MIRT601378 Wdr89 WD repeat domain 89 1 1
MIRT601554 Tapbpl TAP binding protein-like 1 1
MIRT601574 Ssr1 signal sequence receptor, alpha 1 1
MIRT601739 Rbm34 RNA binding motif protein 34 1 1
MIRT601844 Polr2l polymerase (RNA) II (DNA directed) polypeptide L 1 1
MIRT601894 Pbld1 phenazine biosynthesis-like protein domain containing 1 1 1
MIRT601949 Nol3 nucleolar protein 3 (apoptosis repressor with CARD domain) 1 1
MIRT602826 AK010878 GON7, KEOPS complex subunit homolog 1 1
MIRT602859 Rmi2 RecQ mediated genome instability 2 1 1
MIRT602916 1700016D06Rik RIKEN cDNA 1700016D06 gene 1 1
MIRT603001 Myo1c myosin IC 1 1
MIRT603576 Ppm1k protein phosphatase 1K (PP2C domain containing) 1 1
MIRT603581 Ppil4 peptidylprolyl isomerase (cyclophilin)-like 4 1 1
MIRT603702 Nip7 NIP7, nucleolar pre-rRNA processing protein 1 1
MIRT603710 Naip5 NLR family, apoptosis inhibitory protein 5 1 1
MIRT603942 H2-T23 histocompatibility 2, T region locus 23 1 1
MIRT604103 Dph5 diphthamide biosynthesis 5 1 1
MIRT604202 Cpne9 copine family member IX 1 1
MIRT604229 Clca2 chloride channel accessory 3A2 1 1
MIRT604242 Cflar CASP8 and FADD-like apoptosis regulator 1 1
MIRT604392 Aipl1 aryl hydrocarbon receptor-interacting protein-like 1 1 1
MIRT604542 Ypel2 yippee-like 2 (Drosophila) 1 1
MIRT605321 Zfp605 zinc finger protein 605 1 1
MIRT605421 T2 brachyury 2 1 1
MIRT605424 Synrg synergin, gamma 1 1
MIRT605630 Mfsd11 major facilitator superfamily domain containing 11 1 1
MIRT605750 Exosc2 exosome component 2 1 1
MIRT605832 Cenpf centromere protein F 1 1
MIRT606053 Zfp607 zinc finger protein 607A 1 1
MIRT606349 Lcmt2 leucine carboxyl methyltransferase 2 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-674 Glucose NULL 5793 Quantitative real-time PCR endothelial cells 24394957 2014 down-regulated
miR-674 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated

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