miRTarBase - #MIRT596597


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pre-miRNA Information
pre-miRNA mmu-mir-143   
Genomic Coordinates chr18: 61649196 - 61649258
Synonyms Mirn143, mmu-mir-143, Mir143
Description Mus musculus miR-143 stem-loop
Comment Expression of this miRNA in mouse was independently verified in heart and brain .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-143-3p
Sequence 40| UGAGAUGAAGCACUGUAGCUC |60
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B7G3SK miR-143 Predictive Biomarker (PRD); Safety Biomarker (SAF) Clinical/Experimental Data Expression Lower Blood Real-time polymerase chain reaction
B7G3SK miR-143 Predictive Biomarker (PRD); Safety Biomarker (SAF) Transcriptomic Data . . . .
Gene Information
Gene Symbol Zfp488   
Synonyms Gm1206, Znf488
Description zinc finger protein 488
Transcript NM_001013777   
Expression
Putative miRNA Targets on Zfp488
3'UTR of Zfp488
(miRNA target sites are highlighted)		 
>Zfp488|NM_001013777|3'UTR
   1 CAGGTGGCTACAGAGGAAACCGATGGCCATAGTATCCAGGACACAGTGGTGAGGCACAGGTGACTACCGGCCTTTGCTGA
  81 GAAGGTTGTCATAGACTTCTTCAAGGTTGTTCCTTTTGCTTCCCAGACATGAACTGCTCAGTTATAATTTGTCAGGTGTC
 161 CCAGGCCTCGTGATGTGTTTAATGCACAATATGTTTAGTCATGCATTAAAGTGATAAGCTTCACTCATGGGATAGTGGGA
 241 CCCATGGCTCAGGGAACAGGGCTGCTCCTGTCTCTGTCCCCAGTACTCTGCAAGGAAGCACATGAGTATGACATCGTGGC
 321 TTTGCTGATGAGCAAGCCTGCACTCAATCTATGCTTTCAACTATGAGCCCTGACCTCGGACCTGTTCCTTTAGCTTGCTG
 401 AACCTCACTTTTATTTTGAAAGTGGTGTGTTAGCAGGATGTCATCAGAGTGGCCCGCTTATTCCCTGGTTGACTGTGAGC
 481 ATTAAGCATATGGATTGTATAGTCACTGCTTGCACTAGCCAGCTACGTCATTCATGAAATCTCAGGGATGAACAAACATT
 561 GTGTTTCTCTGCCACAAGGCTGGGGATGGCTTGGTGGACATAGCTCAAGCTGCACTCAAGTCTAGGCCCTTTTGCTGCTG
 641 GGTCATTATGCTGGATATAGTGAAGCCCAGAGTGGAAAATACCGAGAAAATGGGTCACAAGCCTAAGTTTCCCTGGCCCT
 721 ACGTAAATCCAGGCCTAAAGGGGCCGAGTGAAAAAGTGACCTGGCTGAAACCCTAACAAAGGAGTGGGGGCTAGTAATAC
 801 AGCTCCCCCCATTCTCTGGGTATTCACACTGTGAATGTGTGAGAAGAGAGAAACAGAACACACATCATTTATTCTGGATT
 881 TTATCCAGGTGCCATTCATCCTTGTGGTTCTGAGTAGGGCAGGAAAGAGCAAGTGGAGGGTCCTCGGAATGAGGTCCCCA
 961 GGCGGCACTTCACATCCTCTCATCTGTAGGACACAGACAGTGAATTCTCATCCTACTGCAGATGAATTTCAACTTAAAAA
1041 GGCTATTGACTTTCCCAACACCACTTTGTCAGGAGCATTGGTGGAAGAACAAAATCCCATGAAGCCCCAGATCTGAGCTC
1121 AGGTCTTTCCAGCCGCTTCATGTCTGCTTAGCTAGCATGGCCATGGAGCGTACTAAGTGACTCAAGGTCTGGAACTTTGA
1201 GGCTGGGCTCCTTAAGTTCAATTCCAACTTAACCTTGCTACTTTACAAGACGTACACGTGCTCATGCACGCGCATGTGCA
1281 CACACACACACACACACACACACACACACACACACACACACACCACTCTTCTCTTGAGCACTTTTCTCCATCTTGTATAC
1361 TAAATAGCATAGCGACTTATCCTGTCCACTGTAGCTCTCCTCTCTAGAATAAAAGGGAGTTTTGGTGTATTTCCATCATC
1441 TAGAACAACACAGACACTCAACAGGTATCTGTTGAAAGAAAGAATAAACACATAAGTTAACAAATGAACGATCTGCCTGA
1521 CTCTGGCTTTGCTCCTGCCAACTCTTGAGTTGGCTTTGAGGAACAAGAGGTGGCATCTGTCAAAAGCACACTTACCTTGA
1601 GCCCACACATTGGCACTGATTTCTATGCCATCTCTTTGGAAAGGGGAATGGTCCTGTCATAGCATACAGAAGATGCTTAG
1681 AATTGATGTTGACACAAGTTGCTTTGCAGCCAGATTTACAAGTTCAAAGATGTTTAAAAGCCGGTGACTTAGGACTTGTT
1761 AGTGACACAGTCCCTCAGAATAAGCATGCTGGGTTCCCAGGTGTCCTCTGGAAGTCCCACCCCCTCTCCTGTCTCCCTTC
1841 TTCATGCCACTTGCTCCTCCCAGCGAGAAGGCACTTTATTTTTATTTTATTTTTATATTTTTCCATTTTTTATTAGGTAT
1921 TTAGCTCATTTACATTTCCAATGTTATACCAAAAGTCCCCCACAGCCACCCACCCCCACTCCCCTACCCACCCACTCCCC
2001 CTTTTTGGCCCTGGCGTTCCCCTGTACTGGGGCATATAAAGTTTGCAAGTCCAATGGGCCTCTCTTTCCAGTGATGGCCG
2081 ACTAGGCCATCTTTTGATACATATGCAGCTAGAGTCAAGAGCTCCGGGGTACTGGTTAGTTCATTATGTTGTTCCACCTA
2161 TAGGGTTGCAGATCCCTTTAGCTCCTTGGCTACTTTCTCTAGCTCCTCCATTGGGAGCCCTGTGATTCATCCATTAGCTG
2241 ACTGTGAGCATCCACTTCTGTGTTTGCTAGGCCCCGGCATAGTCTCACAAGAGACAGCTACATCTGGGTCCTTTCAATAA
2321 AATCTTGCTAGTGTATGCAATGGTCTCAGCGTTTGGAAGCTGATTATGGGGTGGATCCCTGGATATGGCAGTCTCTACAT
2401 GGTCCATCCTTTCATCTCAGCTCCAAACTTTGTCTCTGTAACTCCTTCCATGGGTGTTTTGTTCCCAATTCTAAGGAGGG
2481 GCATAGTGTCCACACTTCAGTCTTCATTCTTCTTGAGTTTCATGTGTTTAGCAAATTGTATCTTATATCTTGGGTATCCT
2561 AGGTTTGGGGCTAATATCCACTTATCAGTGAGTACATATTGTGTGAGTTCCTTTGTGATTGTGTTACCTCACTCAGGATG
2641 ATGCCCTCCAGGTCCATCCATTTGGCTAGGAATTTCATAAATTCATTCTTTTTAATAGCTGAGTAGTACTCCATTGTGTA
2721 GATGTACCACATTTTCTGTATCCATTCCTCTGTTGAGGGGCATCTGGGTTCTTTCCAGCTTCTGGCTATTATAAATAAGG
2801 CTGCTATGAACATAGTGGAGCATGTGTCGAGAAGGCACTTTAAACCATGACTTCCCAACTTAGGTCTTTCATTAAATAAA
2881 TAAAAAAAATAGTCTGGATATTTTTGTTTTTCAAAATTTATGTGAAAATTCTAAGGTGGGCCGTTATGAGATTGGGACTG
2961 ATGCTTTATGGGTTCTAGAGGGCAAGCTCCTCACTGTCTCTCTGAAGACGCAGCTAAAAAGAGCAATCTCTGAACTAGAA
3041 AATGGCCCTCACGAGAGACATTACATCTGCATACACACGACCCCTTTATGATCCAGAACAATAAATTCCTGTTGTTTATA
3121 AAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' cucGAUGU--CACG-----AAGUAGAGu 5'
             |||||  || |     |||||||| 
Target 5' tctCTACATGGTCCATCCTTTCATCTCa 3'
 2392 - 2419 156.00 -15.80
2 
miRNA  3' cucgaUGUCACGA-AGUAGAgu 5'
               ||| | || ||||||  
Target 5' acttcACA-TCCTCTCATCTgt 3'
 967 - 987 131.00 -6.90
3 
miRNA  3' cucGAUGUC-ACGAAGUAGAGu 5'
             :| |||  ||||||| || 
Target 5' tctTTCCAGCCGCTTCATGTCt 3'
 1124 - 1145 130.00 -13.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
24 mmu-miR-143-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004492 Prkce protein kinase C, epsilon 4 1
MIRT004532 Vcan versican 4 1
MIRT004772 Elk1 ELK1, member of ETS oncogene family 4 1
MIRT006031 Kras Kirsten rat sarcoma viral oncogene homolog 5 1
MIRT006032 Rreb1 ras responsive element binding protein 1 5 1
MIRT006121 Ptn pleiotrophin 3 1
MIRT054755 Igfbp5 insulin-like growth factor binding protein 5 2 1
MIRT586427 Ogfrl1 opioid growth factor receptor-like 1 1 1
MIRT589192 Ptpn3 protein tyrosine phosphatase, non-receptor type 3 1 1
MIRT591160 Myo5c myosin VC 1 1
MIRT593574 Zyg11b zyg-ll family member B, cell cycle regulator 1 1
MIRT594328 Nav1 neuron navigator 1 1 1
MIRT595606 Mapk8 mitogen-activated protein kinase 8 1 1
MIRT596433 Crebzf CREB/ATF bZIP transcription factor 1 1
MIRT596597 Zfp488 zinc finger protein 488 1 1
MIRT599868 Acnat1 acyl-coenzyme A amino acid N-acyltransferase 1 1 1
MIRT600661 March5 membrane-associated ring finger (C3HC4) 5 1 1
MIRT602320 Gas2l3 growth arrest-specific 2 like 3 1 1
MIRT604071 Egln2 egl-9 family hypoxia-inducible factor 2 1 1
MIRT731148 Dnmt3a DNA methyltransferase 3A 1 1
MIRT733322 Sox2 SRY (sex determining region Y)-box 2 2 0
MIRT734441 Map3k7 mitogen-activated protein kinase kinase kinase 7 2 0
MIRT735453 COL5A2 collagen type V alpha 2 chain 1 0
MIRT756168 Npy neuropeptide Y 2 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-143 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 up-regulated
miR-143 Trichostatin A (TSA) NULL 444732 Quantitative real-time PCR myocardial differentiation of mouse ES cells 19521018 2009 down-regulated
miR-143 Sodium butyrate NULL 5222465 Quantitative real-time PCR Burkitt lymphoma cells 24577510 2014 up-regulated
miR-143 XMD8-92 NULL 46843772 Microarray pancreatic ductal adenocarcinoma cell 24880079 2014 up-regulated
miR-143 Medpor NULL NULL Microarray osteoblast-like cells line (MG-63) 18408260 2008 down-regulated
miR-143 17beta-estradiol (E2) approved 5757 Microarray MCF-7AKT breast cancer cells 19528081 2009 down-regulated
miR-143 Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-143 Trichostatin A (TSA) NULL 444732 Microarray apoptosis-resistant breast cancer cells 21971930 2011 up-regulated
miR-143 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-143 Estrogen NULL NULL Quantitative real-time PCR breast cancer cells. 22260523 2012 down-regulated
miR-143 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated
miR-143 Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 up-regulated
miR-143 Conjugated linoleic acid NULL NULL Quantitative real-time PCR retroperitoneal adipose tissue 20886002 2010 down-regulated
miR-143 Ursodeoxycholic acid (UDCA) approved 31401 Microarray rat Liver 20689055 2010 up-regulated
miR-143-3p Docosahexaenoic acid NULL 445580 Quantitative real-time PCR Caco-2 cells 24623846 2014 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-143-3p Doxorubicin 31703 NSC123127 approved sensitive Low Osteosarcoma tissue and cell line (SAOS-2, U-2-OS)

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