pre-miRNA Information | |
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pre-miRNA | mmu-mir-677 |
Genomic Coordinates | chr10: 128085286 - 128085363 |
Synonyms | Mirn677, mmu-mir-677, Mir677 |
Description | Mus musculus miR-677 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
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Mature miRNA | mmu-miR-677-5p |
Sequence | 6| UUCAGUGAUGAUUAGCUUCUGA |27 |
Evidence | Experimental |
Experiments | MPSS |
Putative Targets |
Gene Information | |||||||||||||||||||||
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Gene Symbol | Slc25a33 | ||||||||||||||||||||
Synonyms | 5730438N18Rik, Pnc1 | ||||||||||||||||||||
Description | solute carrier family 25, member 33 | ||||||||||||||||||||
Transcript | NM_027460 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Slc25a33 | |||||||||||||||||||||
3'UTR of Slc25a33 (miRNA target sites are highlighted) |
>Slc25a33|NM_027460|3'UTR 1 TGACAGGGCCACATGGTTGTGCTCTAGAAGAATAAAACTGAAAACCCTAGAGATTTTCTTTTCCGTTGATGTTTAGTGTT 81 CAAAACTGAAACAGCGAAGGCCATAGAATACCTGGCTCATGTCACCTGTTGGACATTTCCTTTTGGATTCTTGTTTCTGG 161 AAGGTTGAAATTCATTAACGTTAATAGTTAATTATAACTTTCTTTTTTAACTTAAGAGGACTGAGGATTGAGGAGCAAGT 241 AAATTAAATCATGCTATTTAATGTAAGTTCGTGCTGGGCTGTGTTCTCCTTTGTGCTTACTGGGCTGCCCTAAGCCGGCC 321 ACGGGGTCTGGCTGGGCGTGGGGAAGTGAGAAGCGTGACTTGTGGGGACTGAGTGGTGGACAGGAAAGAGCGGTCACGCT 401 TGGTGACAACTGTGAGCAAATGGTCCACAGCTTTCCCTCTGTACATCTCCATCTCCCCCCTCCATGGTGGTAGGTTTATC 481 TGTAATCTGTTTCCAGACACTGAATGGGGGTGGGGGTGGGGGTGGAGTGTAACTTCCTTAACCACCCCACCCCCCACCCT 561 TTCTGAGTCAGGGTCTTGGTGCTGTAGCCCAGACTGATCTTCACCTTTAAATCTCCTGCCTCAGCCTCCCAAGGGCTGAA 641 AATGCAGATGTACACCCTGGGCCTGACCTAGCTGGACAATAAAAACTGCTTTTAGCTACATT Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | mESCs |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2
... - Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology. |
Article |
- Leung AK; Young AG; Bhutkar A; Zheng GX; et al. - Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
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44 mmu-miR-677-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT580133 | Ubap2l | ubiquitin-associated protein 2-like | ![]() |
1 | 1 | |||||||
MIRT582833 | Itgb3 | integrin beta 3 | ![]() |
1 | 4 | |||||||
MIRT583171 | Grik3 | glutamate receptor, ionotropic, kainate 3 | ![]() |
1 | 3 | |||||||
MIRT583782 | Elovl7 | ELOVL family member 7, elongation of long chain fatty acids (yeast) | ![]() |
1 | 2 | |||||||
MIRT585951 | Sin3a | transcriptional regulator, SIN3A (yeast) | ![]() |
1 | 1 | |||||||
MIRT589768 | Iws1 | IWS1, SUPT6 interacting protein | ![]() |
1 | 1 | |||||||
MIRT591164 | Mup19 | major urinary protein 19 | ![]() |
1 | 1 | |||||||
MIRT591170 | Mup12 | major urinary protein 12 | ![]() |
1 | 1 | |||||||
MIRT591176 | Mup10 | major urinary protein 10 | ![]() |
1 | 1 | |||||||
MIRT591807 | Mup14 | major urinary protein 14 | ![]() |
1 | 1 | |||||||
MIRT592567 | Mup17 | major urinary protein 17 | ![]() |
1 | 1 | |||||||
MIRT592608 | Mup11 | major urinary protein 11 | ![]() |
1 | 1 | |||||||
MIRT593180 | Clasp1 | CLIP associating protein 1 | ![]() |
1 | 1 | |||||||
MIRT594028 | 5430427O19Rik | RIKEN cDNA 5430427O19 gene | ![]() |
1 | 1 | |||||||
MIRT595083 | Zfp516 | zinc finger protein 516 | ![]() |
1 | 1 | |||||||
MIRT595535 | Fign | fidgetin | ![]() |
1 | 1 | |||||||
MIRT595555 | Ubr3 | ubiquitin protein ligase E3 component n-recognin 3 | ![]() |
1 | 1 | |||||||
MIRT595557 | Tcte1 | t-complex-associated testis expressed 1 | ![]() |
1 | 1 | |||||||
MIRT595562 | Sptbn1 | spectrin beta, non-erythrocytic 1 | ![]() |
1 | 1 | |||||||
MIRT595569 | Nrsn1 | neurensin 1 | ![]() |
1 | 1 | |||||||
MIRT595571 | Mtf1 | metal response element binding transcription factor 1 | ![]() |
1 | 1 | |||||||
MIRT595579 | Josd1 | Josephin domain containing 1 | ![]() |
1 | 1 | |||||||
MIRT595580 | Fam101b | refilin B | ![]() |
1 | 1 | |||||||
MIRT595587 | 4930444A02Rik | protein-O-mannose kinase | ![]() |
1 | 1 | |||||||
MIRT595593 | Ago1 | argonaute RISC catalytic subunit 1 | ![]() |
1 | 1 | |||||||
MIRT595602 | Nbl1 | neuroblastoma, suppression of tumorigenicity 1 | ![]() |
1 | 1 | |||||||
MIRT595604 | March8 | membrane-associated ring finger (C3HC4) 8 | ![]() |
1 | 1 | |||||||
MIRT595625 | Nol7 | nucleolar protein 7 | ![]() |
1 | 1 | |||||||
MIRT595629 | Gnptg | N-acetylglucosamine-1-phosphotransferase, gamma subunit | ![]() |
1 | 1 | |||||||
MIRT595633 | Ednra | endothelin receptor type A | ![]() |
1 | 1 | |||||||
MIRT595649 | B3galt5 | UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 5 | ![]() |
1 | 1 | |||||||
MIRT595667 | Rxrb | retinoid X receptor beta | ![]() |
1 | 1 | |||||||
MIRT595743 | Ctdspl2 | CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2 | ![]() |
1 | 1 | |||||||
MIRT595789 | Fignl2 | fidgetin-like 2 | ![]() |
1 | 1 | |||||||
MIRT595880 | Gid4 | GID complex subunit 4, VID24 homolog | ![]() |
1 | 1 | |||||||
MIRT595909 | Tapt1 | transmembrane anterior posterior transformation 1 | ![]() |
1 | 1 | |||||||
MIRT595919 | Trim9 | tripartite motif-containing 9 | ![]() |
1 | 1 | |||||||
MIRT595925 | Efr3a | EFR3 homolog A | ![]() |
1 | 1 | |||||||
MIRT595937 | Acmsd | amino carboxymuconate semialdehyde decarboxylase | ![]() |
1 | 1 | |||||||
MIRT595943 | B3gnt5 | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5 | ![]() |
1 | 1 | |||||||
MIRT596004 | Disp2 | dispatched RND tramsporter family member 2 | ![]() |
1 | 1 | |||||||
MIRT596008 | Ceacam1 | carcinoembryonic antigen-related cell adhesion molecule 1 | ![]() |
1 | 1 | |||||||
MIRT596167 | Ms4a15 | membrane-spanning 4-domains, subfamily A, member 15 | ![]() |
1 | 1 | |||||||
MIRT597273 | Slc25a33 | solute carrier family 25, member 33 | ![]() |
1 | 1 |