pre-miRNA Information | |
---|---|
pre-miRNA | mmu-mir-363 |
Genomic Coordinates | chrX: 52741693 - 52741767 |
Synonyms | Mirn363, mmu-mir-363, Mir363 |
Description | Mus musculus miR-363 stem-loop |
Comment | The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations. |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
---|---|
Mature miRNA | mmu-miR-363-5p |
Sequence | 7| CAGGUGGAACACGAUGCAAUUU |28 |
Evidence | Experimental |
Experiments | Illumina |
Putative Targets |
Gene Information | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | Paxip1 | ||||||||||||||||||||
Synonyms | D5Ertd149e, PTIP | ||||||||||||||||||||
Description | PAX interacting (with transcription-activation domain) protein 1 | ||||||||||||||||||||
Transcript | NM_018878 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Paxip1 | |||||||||||||||||||||
3'UTR of Paxip1 (miRNA target sites are highlighted) |
>Paxip1|NM_018878|3'UTR 1 TGGCCTCTTGCTGCTGCTAGCCTGGAGCCTGGAGCACAAGGGTAGCTGCTGGCAGCATGAACCAGGACTCCCTCCCCGCA 81 GAGGGCTTGTTTTCCAGCTCTGCCTTAGGGAGAGACCTCGACCAGGAAGACAGATGTAATACTGACTGCATCGTGTTAAG 161 ATGTGTGATCTTATTCTAGGAAACATAAATTATGTTTTGTATTATATGCCTAAGAGCCCACATTAAGTCTTCTGATTAAT 241 TTGCCAGGTTTTTAATGTGTTTTCAGAAAGCTGTGTGAAATTTCATCTACAAATAAAAATGATGTAAATAAGACTTTGCT 321 GTCTAAATTATGGCTGTTAAAGATATGAAATGTTTTGTACTTTGATTATTTTTATTTCTTACACTTTTCTTTTATATTGA 401 TAACTTGCTCACAGTTAAATAAATGTACTTTTGAACTTAGAATTGGGTGATTATTTAAAATTTCATATTCTTTGCATAAC 481 ATAAATGATTTTCAACAAGCTGGATAGCATTTTGATAAATTTCACAGCAGAGTATTTTTGAAGTAACTTTTCTCCTGGAG 561 AAATAAGTTTTGATAGTATTTCATAAATAGTCACCTCTTACTGATATGGCAAGGGGGCTCCAGACAGGTGGACTGGAAGC 641 CTGGACAGCCACTAAATGCAAACTGTGAGCCTGTTCCCAGCCTGGGCAGAGCCTACCCTACTGTACTGATGTTGTACTCC 721 AAAGCATGGTGGGGACAGTGCTCTGTTCTACGGGAAGGCCCGGTGTCTTTTGCCTACATGTTTCCTGGCTAGCCCTGATC 801 TCTGGTGACTTTGCATAGGACTGTTACTATCTGCAGAAACATCCTTCTGTTGGACATTCCTTGTGGCAGGTGTGTCTTCA 881 CAGGCTGCTTTGGAGAGGCTGTCTAAGCAACCTGAGAGACTTCCTACAGAGCTGGCCTTTATCTGGTTGAAGTTGTCACT 961 ATGAAATACTTCCAAATGTGGAAATTGGGGTGAGCAGAAGAAGAGCTCTGTACGTGGGGACAGTCGGATTTGCCAGCATG 1041 CCACATGCCATCCTGTGTCCTTGATCCTAGAACAAAGTGGATGTAGATTCAATAGCCTTCTCTGTGGAATGTCACACATC 1121 TGTTACCTGTGCAGGAGACAGGATGTTTCTTTAAGAGAGATATGACTGATTTTTTTTTTTTTCTGGTTTTTCGAGACAGG 1201 GTTTCTCTGTGTAGCTCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAGAAATCCACCTGCCTC 1281 TGCCTCTCAAGTGCTGGGATTAAAGGCGTGGGCCACCACGCCCAGCTTGATTTTTTTTTTTATTTATGTGTATGAGTACT 1361 CTGTTGCTGTCTTCAGACACTCCAGAAGAGGGCATCCAATTCCAATTCCATTACAGATGGTAATGAGCCACCATGTGGTT 1441 GCTGGGAATTGAACTCAGGACCTCTGGAAGAGCAGTCAGCGCTCTTCCAGCCCTTGATTAACCTTTCAATTAGCATCTTC 1521 TGTAAGCTGCCAAGAAATGCAACTTGAGGAGCTGTCCAGAACCACAGCTTACACAGCTGTTCCACCCGAGGTTGGCTGGG 1601 AGGGCAAGGTGACAAACAAATGCTGGTTATGAAGTAAATCAGAAAGGTGGTGGTTTCAAAAATGGTGCACATCTGGAGTG 1681 CCAACAGTCGAATGCTGAGATACAGTGTTCTACATGGCAGACCCTGGGCTATCCAGGGCTACATGGGTGAGATCCTGTCT 1761 CAAAAAGTAAATAATTATATTTTGCCCACATTGTTTAAAATACAGTTAACTCTTTTCTGGTAATTGTATCTGTTTTTAAT 1841 TAATGTTTTATAACATCAAAGTTTCAGTCTCTGACCCCTAGGTACATGTTAGTGCTCAGCTGGTTACGTTTGATGGTGAA 1921 CAGTCATCATGAATACATCACTTGTAGTCCTGTGATTTGCAACACTGGCCAGAGGTAGTAGGTGGAAGGTTAGTGGCAGG 2001 ACTCAGGGGGTGTGTTCAAACCATTACAGAATGGTTTCATTGTTTCCTGAAGTTAAAGGGGAGTGACCTGTGCGGTTTTG 2081 GTTTGGTTTGGGAGGAAAGAGTGCCTTGGCACTCTTTGATAGTCTTTAAAGATCTGGTAGTCTTTAGTCTTGGAGTCTGG 2161 AGTGTCAGGAACCATCACCACACACCATCAGTGGGTTTTCAACAAAGTGCTTAAGTTGCATAGGAAAGTTTCTAGTAGAA 2241 GCTGCACAGCTTGCTTCCTTTAAACATTTTTACTGGAAATTGGAACACAGTTCATCAGAAGGTATCTGTCCTGGGCACCT 2321 TCTGAGAGTGCTGTTTCACTCCACAAAGCATTTGTGTAATAATAAAAAAAAAAAATCAGTATTAAAGTCCTGATGCTGAG 2401 AGTCTAAAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
||||||||||||||||||||
miRNA-target interactions (Predicted by miRanda) |
|
Experimental Support 1 for Functional miRNA-Target Interaction | |
---|---|
miRNA:Target | ---- |
Validation Method |
|
Conditions | mESCs |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B
HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2
HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1
HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2
... - Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology. |
Article |
- Leung AK; Young AG; Bhutkar A; Zheng GX; et al. - Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
|
CLIP-seq Support 1 for dataset GSM4751756 | |
---|---|
Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | adipose tissue / RNA sequencing of iBAT 1 |
Location of target site | NM_018878 | 3UTR | CUGGUUUUUCGAGACAGGGUUUCUCUGUGUAGCUCUGGCUGUCCUGGAACUCACUUUGUAGACCAGGCUGGCCUCGAACUCAGAAAUCC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE142677 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM622571 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | mESCs / WT1B |
Location of target site | NM_018878 | 3UTR | UCCACCUGCCUCUGCCUCUCAAGUGCUGGGAUUAAAGGCGUGGGCC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21258322 / GSE25310 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset GSM622572 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | mESCs / WT2 |
Location of target site | NM_018878 | 3UTR | CCACCUGCCUCUGCCUCUCAAGUGCUGGGAUUAAAGGCGUGGGCC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21258322 / GSE25310 |
CLIP-seq Viewer | Link |
CLIP-seq Support 4 for dataset GSM622573 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | mESCs / KO1 |
Location of target site | NM_018878 | 3UTR | AGAAAUCCACCUGCCUCUGCCUCUCAAGUGCUGGGAUUAAAGGCGUGGGCC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21258322 / GSE25310 |
CLIP-seq Viewer | Link |
CLIP-seq Support 5 for dataset GSM622574 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | mESCs / KO2 |
Location of target site | NM_018878 | 3UTR | AGAAAUCCACCUGCCUCUGCCUCUCAAGUGCUGGGAUUAAAGGCGUGGGCCACC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21258322 / GSE25310 |
CLIP-seq Viewer | Link |
76 mmu-miR-363-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
![]() |
![]() |
|||||||
Strong evidence | Less strong evidence | |||||||||||
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
|||||
MIRT577239 | Zfp933 | zinc finger protein 933 | ![]() |
![]() |
2 | 4 | ||||||
MIRT578026 | Pfas | phosphoribosylformylglycinamidine synthase (FGAR amidotransferase) | ![]() |
![]() |
2 | 2 | ||||||
MIRT578034 | Pde4c | phosphodiesterase 4C, cAMP specific | ![]() |
![]() |
2 | 2 | ||||||
MIRT578102 | Nup133 | nucleoporin 133 | ![]() |
![]() |
2 | 2 | ||||||
MIRT578143 | Nfatc4 | nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 4 | ![]() |
![]() |
2 | 6 | ||||||
MIRT578431 | Nmrk2 | nicotinamide riboside kinase 2 | ![]() |
1 | 1 | |||||||
MIRT579036 | Cts8 | cathepsin 8 | ![]() |
![]() |
2 | 4 | ||||||
MIRT579251 | Car5b | carbonic anhydrase 5b, mitochondrial | ![]() |
![]() |
2 | 4 | ||||||
MIRT579607 | 4930519G04Rik | RIKEN cDNA 4930519G04 gene | ![]() |
![]() |
2 | 4 | ||||||
MIRT581397 | Rbms2 | RNA binding motif, single stranded interacting protein 2 | ![]() |
![]() |
2 | 4 | ||||||
MIRT585497 | Ttc26 | tetratricopeptide repeat domain 26 | ![]() |
![]() |
2 | 4 | ||||||
MIRT585507 | Tspan31 | tetraspanin 31 | ![]() |
![]() |
2 | 4 | ||||||
MIRT585583 | Tox4 | TOX high mobility group box family member 4 | ![]() |
![]() |
2 | 4 | ||||||
MIRT585842 | Slc3a2 | solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT586452 | Nploc4 | NPL4 homolog, ubiquitin recognition factor | ![]() |
![]() |
2 | 4 | ||||||
MIRT587010 | Gngt1 | guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1 | ![]() |
![]() |
2 | 4 | ||||||
MIRT588115 | Aco1 | aconitase 1 | ![]() |
![]() |
2 | 4 | ||||||
MIRT589171 | Rab11b | RAB11B, member RAS oncogene family | ![]() |
![]() |
2 | 2 | ||||||
MIRT589338 | Ostf1 | osteoclast stimulating factor 1 | ![]() |
![]() |
2 | 4 | ||||||
MIRT589488 | Mzt1 | mitotic spindle organizing protein 1 | ![]() |
![]() |
2 | 4 | ||||||
MIRT589617 | Lrrc58 | leucine rich repeat containing 58 | ![]() |
![]() |
2 | 4 | ||||||
MIRT589816 | Hnrnpab | heterogeneous nuclear ribonucleoprotein A/B | ![]() |
![]() |
2 | 4 | ||||||
MIRT590086 | Evi2b | ecotropic viral integration site 2b | ![]() |
![]() |
2 | 2 | ||||||
MIRT590799 | 2810474O19Rik | RIKEN cDNA 2810474O19 gene | ![]() |
![]() |
2 | 2 | ||||||
MIRT590993 | Slc28a3 | solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 | ![]() |
![]() |
2 | 4 | ||||||
MIRT592906 | Mmachc | methylmalonic aciduria cblC type, with homocystinuria | ![]() |
![]() |
2 | 4 | ||||||
MIRT593320 | Slc25a51 | solute carrier family 25, member 51 | ![]() |
1 | 2 | |||||||
MIRT594758 | Ttn | titin | ![]() |
![]() |
2 | 2 | ||||||
MIRT594872 | Magt1 | magnesium transporter 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT595323 | Gfap | glial fibrillary acidic protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT595614 | Abce1 | ATP-binding cassette, sub-family E (OABP), member 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT596863 | Ttc23 | tetratricopeptide repeat domain 23 | ![]() |
![]() |
2 | 2 | ||||||
MIRT597329 | Senp5 | SUMO/sentrin specific peptidase 5 | ![]() |
![]() |
2 | 2 | ||||||
MIRT597745 | Polr1b | polymerase (RNA) I polypeptide B | ![]() |
![]() |
2 | 2 | ||||||
MIRT597779 | Plin3 | perilipin 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT597900 | Paxip1 | PAX interacting (with transcription-activation domain) protein 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT598014 | Nop16 | NOP16 nucleolar protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT598196 | Mrgprb1 | MAS-related GPR, member B1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT598360 | Man2a2 | mannosidase 2, alpha 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT598933 | Git2 | G protein-coupled receptor kinase-interactor 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT599064 | Epb4.2 | erythrocyte membrane protein band 4.2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT599072 | Eif2b1 | eukaryotic translation initiation factor 2B, subunit 1 (alpha) | ![]() |
![]() |
2 | 2 | ||||||
MIRT599119 | Dynll1 | dynein light chain LC8-type 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT599271 | D14Abb1e | family with sequence similarity 208, member A | ![]() |
![]() |
2 | 2 | ||||||
MIRT599613 | Birc5 | baculoviral IAP repeat-containing 5 | ![]() |
![]() |
2 | 2 | ||||||
MIRT599909 | Abcc9 | ATP-binding cassette, sub-family C (CFTR/MRP), member 9 | ![]() |
![]() |
2 | 2 | ||||||
MIRT600060 | Smco1 | single-pass membrane protein with coiled-coil domains 1 | ![]() |
1 | 1 | |||||||
MIRT600067 | Knop1 | lysine rich nucleolar protein 1 | ![]() |
1 | 1 | |||||||
MIRT600320 | Stxbp5l | syntaxin binding protein 5-like | ![]() |
![]() |
2 | 2 | ||||||
MIRT600337 | Strbp | spermatid perinuclear RNA binding protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT600524 | Ppargc1a | peroxisome proliferative activated receptor, gamma, coactivator 1 alpha | ![]() |
![]() |
2 | 2 | ||||||
MIRT600646 | Med18 | mediator complex subunit 18 | ![]() |
![]() |
2 | 2 | ||||||
MIRT600683 | Mapre1 | microtubule-associated protein, RP/EB family, member 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT600802 | C5ar2 | complement component 5a receptor 2 | ![]() |
1 | 1 | |||||||
MIRT601265 | 9930104L06Rik | RIKEN cDNA 9930104L06 gene | ![]() |
![]() |
2 | 2 | ||||||
MIRT601726 | Rfxank | regulatory factor X-associated ankyrin-containing protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT601751 | Rasl12 | RAS-like, family 12 | ![]() |
![]() |
2 | 2 | ||||||
MIRT601888 | Pctp | phosphatidylcholine transfer protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT602148 | Irgm2 | immunity-related GTPase family M member 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT602904 | Fam219b | family with sequence similarity 219, member B | ![]() |
1 | 1 | |||||||
MIRT603138 | Ufd1l | ubiquitin recognition factor in ER-associated degradation 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT603213 | Tnfrsf26 | tumor necrosis factor receptor superfamily, member 26 | ![]() |
![]() |
2 | 2 | ||||||
MIRT603268 | Svopl | SV2 related protein homolog (rat)-like | ![]() |
![]() |
2 | 2 | ||||||
MIRT603291 | Spib | Spi-B transcription factor (Spi-1/PU.1 related) | ![]() |
![]() |
2 | 2 | ||||||
MIRT603922 | Hist1h2br | histone cluster 1 H2br | ![]() |
![]() |
2 | 2 | ||||||
MIRT603924 | Hist1h2bq | histone cluster 1, H2bq | ![]() |
![]() |
2 | 2 | ||||||
MIRT604014 | Fut4 | fucosyltransferase 4 | ![]() |
![]() |
2 | 2 | ||||||
MIRT604105 | Dll3 | delta-like 3 (Drosophila) | ![]() |
![]() |
2 | 2 | ||||||
MIRT604166 | Cyb5d2 | cytochrome b5 domain containing 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT604313 | Ccdc127 | coiled-coil domain containing 127 | ![]() |
![]() |
2 | 2 | ||||||
MIRT605041 | Efr3b | EFR3 homolog B | ![]() |
![]() |
2 | 2 | ||||||
MIRT605716 | Gpr157 | G protein-coupled receptor 157 | ![]() |
![]() |
2 | 2 | ||||||
MIRT605741 | Gipc1 | GIPC PDZ domain containing family, member 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT605747 | Fam96a | family with sequence similarity 96, member A | ![]() |
![]() |
2 | 2 | ||||||
MIRT606125 | Tnfrsf10b | tumor necrosis factor receptor superfamily, member 10b | ![]() |
![]() |
2 | 2 | ||||||
MIRT606693 | Cnnm3 | cyclin M3 | ![]() |
![]() |
2 | 2 |
miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|