pre-miRNA Information | |
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pre-miRNA | mmu-mir-674 |
Genomic Coordinates | chr2: 117185127 - 117185226 |
Synonyms | Mirn674, mmu-mir-674, Mir674 |
Description | Mus musculus miR-674 stem-loop |
Comment | Landgraf et al. show that the 5' miRNA product is the predominant one . |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
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Mature miRNA | mmu-miR-674-5p |
Sequence | 25| GCACUGAGAUGGGAGUGGUGUA |46 |
Evidence | Experimental |
Experiments | MPSS |
Putative Targets |
Gene Information | |||||||||||||||||||||
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Gene Symbol | Nup133 | ||||||||||||||||||||
Synonyms | mermaid | ||||||||||||||||||||
Description | nucleoporin 133 | ||||||||||||||||||||
Transcript | NM_172288 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Nup133 | |||||||||||||||||||||
3'UTR of Nup133 (miRNA target sites are highlighted) |
>Nup133|NM_172288|3'UTR
1 CATTTGTAGAAATGGTTGTTTCTAAAATTTGTGTAAATATTTGCTTATTAAAAAAAAATATTGAGGCCAAAAAACTTAGG
81 TCTGGATCAACCTGAAGTCTGTTTTGTCTGCAGCAACCAGGTTTTCTATGCTTTATATGGGAGCCGCGATATAAAAGGAG
161 GGGCCCTGATGCTCTTTTTCAGCTGTAAGGTATTTCCTCCTCCTCTTCCACCTGAAGTCACCCTTGCTTCTGTGTAGCTG
241 CCCTTCTCCAGCCTCCCTCGGTCCAAAGAGGCAGCAAAACAAAACAGGTTGCCTCGCTCCCCAACTGGTTGTCCAATACC
321 AGCCCTGAAAAAATATAAGAATGTGTATAATAATATACATGAATTTACATGCAAGTAACGTTCTATGGGCTGAGCAAGTG
401 TGTCTGTTTATTTGGGTCAGCAATTAAAAGATGCTACAGGTTGGAGAGCAGGGCAGCGGAAGTGAGGAAGTGTTGGAGAT
481 GGCAGGGGACGGGGAAAACAGATTGCCCTCTGCATGTGGCACTCTGGGAGTCTCTGAGAAGCTGATCTCTTGGTCGGAGG
561 CAAAGGGAAGTGCCTGCTTAGCAAAGTGAAGATGAGATGTCACTGATTTATACTGTGGCCTAGAAACCAAAACCCTGGGT
641 CTAACTCGGGAGGGTGGGCAGCTGGTAACTCACTGGGAATGTTCTCACTTCCTACATTGATTTAAAAATTACAAGCAAGG
721 GGCAGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCGCTGTCTACTCTTCCAGAGGACTTGAATTCAATTCCCAGCAACCA
801 CATGGTGGCTCACTACCATCTGTAATAGGTCCCAGGGCCCTCTTCTGGTGTGTCTGAAGACAGTGACAGTGTACTCACAT
881 ATATAAAATAAATCTTTTTTAAAAAAAAAGTTAAAACTTAAAAGCCAAAAAAGCCATATATATATGTGTGTGTGTGTGTG
961 TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAAATTAAAGGATTAAAAGTGTGGGCCACTGCATCCATCAATAATGG
1041 ATAAGTCTTAAAGTGTTTTAGTAAGATGTTTGTGTGGCTTTCTGAGCTGTCAGTAAGTTGTCATTGCTACCAGATATGGT
1121 ACTGGCATTGTCTGTGACCCCAGTATTGACTCATAAACCTAGGATGGTTTCTAGTCTCACCGTAGACGGTCCCTTCCTAA
1201 ACATCGTAATGCCAAGGCTGGTCTACCCAGAGATGTTGCTCTGAGAGCTCTGGCCATGGCCTACCTGTCTGAAGAAGGGG
1281 GCTAAACCTTTATGGCAACTATCGCAGCTTCAGAATGGTTGGCATGTAAACTTACTCCAGCAGTAAAAATGAAATTAAGA
1361 CTTTCAGTGTTGAATATCTTTCCCTCCTAAGTTTTAAAAACTGGTCACATAATCCATCTCTGAAGAATGAATGATTTGTT
1441 TCTAAGTCAGGTAGCTAAGACTGTCTCCTGGTACCCGGTGTACATTCTTGGGATCCTTCTCTTGTTGGCCTATCCGTTAG
1521 TTCAGCATGAACTAGTTAGGACTGTGAATGGCAGCAGCCACCCTTGTCCCTGAGGTGAGTGTGCTGTATTCTGAGGATGT
1601 GTGAGTCAGTCAGGACCTGAAAGGGCCCTGGCAACCTGTACAGAGCACAGGGGGAAAGGTTTTGTTGTTTTATTCTCTTG
1681 ACTGGATGCTATGGGGAGAGCCAGAGGTCTGCAAGACAAATTCATGGTGTTTCGGGCTGTCATGGTTGGTCCCACATTCT
1761 GAGTGTTCCCAGTGTGGCCTCCCCCACCACTGCCAGTCTGTCCTCAGAATGGCATAATATTCATGTATAAAATACAATAT
1841 ATGTTGAAAAACAATGTGTATATATGTTGTATATATTTATCATATGTGTATGATATGTGCATATAAACTCTGGCTGGCCT
1921 CAAACTCTCAGCCCCTGTGCCTCAGCTTCCCAAGTCCTAAAACTGCAAGTGTACACACCAGCCCTGGCTAGAACAATTTT
2001 TTTAAGGCGTGTGAAGCCTGACATTGAGCTCATATCCAAAATAAACACAAGTAACTGGTGGACCTGGAAGCTGGGCTTCT
2081 GTTTTGAAATGAGCTTCAGTCGACCACCTTTTGTATTGTGTGGGCAATGGTGAGTACTTAGCACACCTTTGGACTTCTGT
2161 TCTCAGAGGAGCTCCAGTTTTCCTTCTCCTGCTGGGATGGCAGGTCCTTGCATGCATCTGCATTGCCTTAATAAAAACGA
2241 GGTTTTCTTTT
Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | mESCs |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A
HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B
HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2
HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1
HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2
... - Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology. |
Article |
- Leung AK; Young AG; Bhutkar A; Zheng GX; et al. - Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
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94 mmu-miR-674-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT577119 | Prps2 | phosphoribosyl pyrophosphate synthetase 2 | ![]() |
1 | 3 | |||||||
MIRT579212 | Ccdc25 | coiled-coil domain containing 25 | ![]() |
1 | 4 | |||||||
MIRT580735 | Srp72 | signal recognition particle 72 | ![]() |
1 | 2 | |||||||
MIRT581673 | Ppp2r2c | protein phosphatase 2, regulatory subunit B, gamma | ![]() |
1 | 1 | |||||||
MIRT582930 | Il15ra | interleukin 15 receptor, alpha chain | ![]() |
1 | 1 | |||||||
MIRT584530 | Bzrap1 | TSPO associated protein 1 | ![]() |
1 | 1 | |||||||
MIRT584648 | Bach2 | BTB and CNC homology, basic leucine zipper transcription factor 2 | ![]() |
1 | 1 | |||||||
MIRT586666 | Mbp | myelin basic protein | ![]() |
1 | 2 | |||||||
MIRT588993 | Rpl7l1 | ribosomal protein L7-like 1 | ![]() |
1 | 1 | |||||||
MIRT589588 | Magt1 | magnesium transporter 1 | ![]() |
1 | 1 | |||||||
MIRT590893 | Tmem69 | transmembrane protein 69 | ![]() |
1 | 2 | |||||||
MIRT590965 | Slfn5 | schlafen 5 | ![]() |
1 | 2 | |||||||
MIRT591089 | Pola2 | polymerase (DNA directed), alpha 2 | ![]() |
1 | 2 | |||||||
MIRT591253 | Loxl2 | lysyl oxidase-like 2 | ![]() |
1 | 2 | |||||||
MIRT591405 | Churc1 | churchill domain containing 1 | ![]() |
1 | 2 | |||||||
MIRT591425 | Casc4 | cancer susceptibility candidate 4 | ![]() |