pre-miRNA Information
pre-miRNA mmu-mir-674   
Genomic Coordinates chr2: 117185127 - 117185226
Synonyms Mirn674, mmu-mir-674, Mir674
Description Mus musculus miR-674 stem-loop
Comment Landgraf et al. show that the 5' miRNA product is the predominant one .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-674-5p
Sequence 25| GCACUGAGAUGGGAGUGGUGUA |46
Evidence Experimental
Experiments MPSS
Putative Targets

Gene Information
Gene Symbol Nup133   
Synonyms mermaid
Description nucleoporin 133
Transcript NM_172288   
Expression
Putative miRNA Targets on Nup133
3'UTR of Nup133
(miRNA target sites are highlighted)
>Nup133|NM_172288|3'UTR
   1 CATTTGTAGAAATGGTTGTTTCTAAAATTTGTGTAAATATTTGCTTATTAAAAAAAAATATTGAGGCCAAAAAACTTAGG
  81 TCTGGATCAACCTGAAGTCTGTTTTGTCTGCAGCAACCAGGTTTTCTATGCTTTATATGGGAGCCGCGATATAAAAGGAG
 161 GGGCCCTGATGCTCTTTTTCAGCTGTAAGGTATTTCCTCCTCCTCTTCCACCTGAAGTCACCCTTGCTTCTGTGTAGCTG
 241 CCCTTCTCCAGCCTCCCTCGGTCCAAAGAGGCAGCAAAACAAAACAGGTTGCCTCGCTCCCCAACTGGTTGTCCAATACC
 321 AGCCCTGAAAAAATATAAGAATGTGTATAATAATATACATGAATTTACATGCAAGTAACGTTCTATGGGCTGAGCAAGTG
 401 TGTCTGTTTATTTGGGTCAGCAATTAAAAGATGCTACAGGTTGGAGAGCAGGGCAGCGGAAGTGAGGAAGTGTTGGAGAT
 481 GGCAGGGGACGGGGAAAACAGATTGCCCTCTGCATGTGGCACTCTGGGAGTCTCTGAGAAGCTGATCTCTTGGTCGGAGG
 561 CAAAGGGAAGTGCCTGCTTAGCAAAGTGAAGATGAGATGTCACTGATTTATACTGTGGCCTAGAAACCAAAACCCTGGGT
 641 CTAACTCGGGAGGGTGGGCAGCTGGTAACTCACTGGGAATGTTCTCACTTCCTACATTGATTTAAAAATTACAAGCAAGG
 721 GGCAGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCGCTGTCTACTCTTCCAGAGGACTTGAATTCAATTCCCAGCAACCA
 801 CATGGTGGCTCACTACCATCTGTAATAGGTCCCAGGGCCCTCTTCTGGTGTGTCTGAAGACAGTGACAGTGTACTCACAT
 881 ATATAAAATAAATCTTTTTTAAAAAAAAAGTTAAAACTTAAAAGCCAAAAAAGCCATATATATATGTGTGTGTGTGTGTG
 961 TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTAAAATTAAAGGATTAAAAGTGTGGGCCACTGCATCCATCAATAATGG
1041 ATAAGTCTTAAAGTGTTTTAGTAAGATGTTTGTGTGGCTTTCTGAGCTGTCAGTAAGTTGTCATTGCTACCAGATATGGT
1121 ACTGGCATTGTCTGTGACCCCAGTATTGACTCATAAACCTAGGATGGTTTCTAGTCTCACCGTAGACGGTCCCTTCCTAA
1201 ACATCGTAATGCCAAGGCTGGTCTACCCAGAGATGTTGCTCTGAGAGCTCTGGCCATGGCCTACCTGTCTGAAGAAGGGG
1281 GCTAAACCTTTATGGCAACTATCGCAGCTTCAGAATGGTTGGCATGTAAACTTACTCCAGCAGTAAAAATGAAATTAAGA
1361 CTTTCAGTGTTGAATATCTTTCCCTCCTAAGTTTTAAAAACTGGTCACATAATCCATCTCTGAAGAATGAATGATTTGTT
1441 TCTAAGTCAGGTAGCTAAGACTGTCTCCTGGTACCCGGTGTACATTCTTGGGATCCTTCTCTTGTTGGCCTATCCGTTAG
1521 TTCAGCATGAACTAGTTAGGACTGTGAATGGCAGCAGCCACCCTTGTCCCTGAGGTGAGTGTGCTGTATTCTGAGGATGT
1601 GTGAGTCAGTCAGGACCTGAAAGGGCCCTGGCAACCTGTACAGAGCACAGGGGGAAAGGTTTTGTTGTTTTATTCTCTTG
1681 ACTGGATGCTATGGGGAGAGCCAGAGGTCTGCAAGACAAATTCATGGTGTTTCGGGCTGTCATGGTTGGTCCCACATTCT
1761 GAGTGTTCCCAGTGTGGCCTCCCCCACCACTGCCAGTCTGTCCTCAGAATGGCATAATATTCATGTATAAAATACAATAT
1841 ATGTTGAAAAACAATGTGTATATATGTTGTATATATTTATCATATGTGTATGATATGTGCATATAAACTCTGGCTGGCCT
1921 CAAACTCTCAGCCCCTGTGCCTCAGCTTCCCAAGTCCTAAAACTGCAAGTGTACACACCAGCCCTGGCTAGAACAATTTT
2001 TTTAAGGCGTGTGAAGCCTGACATTGAGCTCATATCCAAAATAAACACAAGTAACTGGTGGACCTGGAAGCTGGGCTTCT
2081 GTTTTGAAATGAGCTTCAGTCGACCACCTTTTGTATTGTGTGGGCAATGGTGAGTACTTAGCACACCTTTGGACTTCTGT
2161 TCTCAGAGGAGCTCCAGTTTTCCTTCTCCTGCTGGGATGGCAGGTCCTTGCATGCATCTGCATTGCCTTAATAAAAACGA
2241 GGTTTTCTTTT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' auguggugaggguaGAGUCACg 5'
                        ||||||| 
Target 5' ggctggagagatggCTCAGTGg 3'
726 - 747 140.00 -10.50
2
miRNA  3' auGUGG-----UGAGGGU-AGAGUCACg 5'
            ||||     |||:|:: |||||| | 
Target 5' caCACCTTTGGACTTCTGTTCTCAGAGg 3'
2142 - 2169 132.00 -17.50
3
miRNA  3' auguggugaggguAGAGUCACg 5'
                       |:|||||| 
Target 5' atgaaattaagacTTTCAGTGt 3'
1349 - 1370 129.00 -9.50
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1 HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
94 mmu-miR-674-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577119 Prps2 phosphoribosyl pyrophosphate synthetase 2 1 3
MIRT579212 Ccdc25 coiled-coil domain containing 25 1 4
MIRT580735 Srp72 signal recognition particle 72 1 2
MIRT581673 Ppp2r2c protein phosphatase 2, regulatory subunit B, gamma 1 1
MIRT582930 Il15ra interleukin 15 receptor, alpha chain 1 1
MIRT584530 Bzrap1 TSPO associated protein 1 1 1
MIRT584648 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 1 1
MIRT586666 Mbp myelin basic protein 1 2
MIRT588993 Rpl7l1 ribosomal protein L7-like 1 1 1
MIRT589588 Magt1 magnesium transporter 1 1 1
MIRT590893 Tmem69 transmembrane protein 69 1 2
MIRT590965 Slfn5 schlafen 5 1 2
MIRT591089 Pola2 polymerase (DNA directed), alpha 2 1 2
MIRT591253 Loxl2 lysyl oxidase-like 2 1 2
MIRT591405 Churc1 churchill domain containing 1 1 2
MIRT591425 Casc4 cancer susceptibility candidate 4