miRTarBase - #MIRT598598


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pre-miRNA Information
pre-miRNA mmu-mir-92a-1   
Genomic Coordinates chr14: 115044427 - 115044506
Description Mus musculus miR-92a-1 stem-loop
Comment The predominant miR-92 form cloned by Landgraf et al. has a additional 3' U residue, which is compatible with this precursor sequence, but not with that of mir-92-2 (MIR:MI0000580) .
RNA Secondary Structure
pre-miRNA mmu-mir-92a-2   
Genomic Coordinates chrX: 52741838 - 52741928
Description Mus musculus miR-92a-2 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-92a-3p
Sequence 50| UAUUGCACUUGUCCCGGCCUG |70
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
BE2BW7 miR-92a-3p Monitoring Biomarker (MOI) Clinical/Experimental Data Expression Increase Plasma Polymerase chain reaction
Gene Information
Gene Symbol Il5ra   
Synonyms CD125, CDw125, Il5r
Description interleukin 5 receptor, alpha
Transcript NM_008370   
Expression
Putative miRNA Targets on Il5ra
3'UTR of Il5ra
(miRNA target sites are highlighted)		 
>Il5ra|NM_008370|3'UTR
   1 TGGCATTTTGCCATTCTGAAATGAACTCATACAGGACTCCGTGATAAGAGCAAGGACTGCTATTTCTTGGCAAGGAGGTA
  81 TTTCAAATGAACACTCAGAGCCAGGCGGTGGTGGAGCTCGCCTTTAATACCAGCACCTGGGATGCACAGATGGGAGGATT
 161 TCTGAGTTCGAGGCCAGCTTGGTCTATAAAGTGAGTTCCAGGACAGCCAGAGCTACACAGAGAAACCCTGTCTCGAAAAA
 241 ACAAACAAACAAACAAACAAAAATGAACACTCAGTTTGAATGCAAGTCACCAACCCATCCAGACATGAGTCACCAATGTC
 321 CCATTTCATAAAGTGTGCATGCCTCACTCAAACCTCCTTGCTCACAGCATAGCACCAGACTCACCCAGAGCATGGGCCTT
 401 TGGTTTCCTACCCAGAGTACCATGTTATACCAGTGTGTCTTTGAAAGTTGCTTGACTTACCTTGAACTTTTTGCACAGGA
 481 GACAGTTTTTTTAAGCTAATGTCACACATGTTTACTTTGGGTTAAGTTGCCAGTGGTAGCACTCAGCTACAGTGACAGGA
 561 GGAAAGGATAGAACTCATTGAGAGTGAACCCAAATTCAAGACTGTCTTTCCTGAAGCAAGTGGGAGACACAATTTCATGG
 641 TGCTTTTCCCCTTTGAGTTCTAGAATAGTTTTCTTTCTAGAACTGTGCCTGTGTCTTAAAGCATAAGGTAACATTGAGGC
 721 AAAAACAAAGACTATGTCCCACATGTCCCTGTGTTCCATAGGCCTGTTCAAGGAAATGTCTAAGCCAAAGTAAGTTTAAG
 801 TCACCGTGCCTGGGGTGAAAAAGATGGTTCAGATGACGAAGAAGCATGAGGGCCTGAGATTGATCAACCAGCATCAAGAA
 881 ACAACAACAACAACAGCAGCAGCAACAACAAAACAGTGCAAGAAGCACATTCCTATAACCCCAGAGTTGGGAGATAAAGA
 961 CAAGAGGATCCATGGGAATTGTAGTTCAACCAGTTTAGCCAATTATGTTTTCTCTAGGTTCACTGAGAGAAATGGTCTTA
1041 AAAATTTAAGGTGGAGAGTGACTAGGCAGATCCTCTGATACTGACTTCTGCCCTAAATATGCATACACATGTACACACAC
1121 AACACAAAGACACCATTCCCTATTGAGAGAGAAGACAGAAGCTTGTTCAAGGATTAAATTCTTCAAGGCTTCTAGGTACT
1201 CTGGAAATGACCTGAGAAAGACATTGAAAATAATTCTGCTTTGGAGGTGATTGCTGGATCTAGAATGTACTTCCCAAAGA
1281 GATGTTTGATGAAAGAGCCTTCATGGCAACCTGTTGGTCAACTCATGCTTAGTCAATTCTAATCTCTTAAATTAGGGTTT
1361 CCTATACATATTACAATTGTATAAAAATGTATTCTCTAAATATCTTCATTAATGAAGCTGTATCTATAGGTCTTTTTGAT
1441 GGGCTGAACATAGAAGCAAACACACTTATGTGTTGGGAAGAGGAATAAGTAGTGATAGAGGGACCTAGTGGTGGTTATTT
1521 TACATAGTCCTGAAGAGCTAAAGACAATGAAAGAAGAAATGGTACTCACAAGAGAGAGAGCTATGTCGGGGTCCTGTCAG
1601 CCAAATCTTGCTAGTATATGCAATAGTGTCTGGGTTTGGTGGTTGTATATGGGATGGATCCCTGGGTGGGGCAGTCTCTG
1681 GATGGTCTTTCCTTCCATCACAGCTCTGAAATTTGTCTCTGTAACTCCTTCCATGAGTATTTTGTTCCCCATTCTAAGAA
1761 GCAGTGAAGTATCCACACTTTGGTCTTCCTTCTTCTTGAGTTTCATGTGTTTTGCAAATTGTGTGCCTGGCAAATACAGA
1841 AGCAGATGCTCACAGTCATCTATTGGATGAAACACAGGGCCCCTAATGAAGGAGCCAGAGAAAGTACCCAAGGAGCTAAA
1921 AGGGTCTGCAACCCTATAGCAGGAACAACAATATGAACTACCCAGCAACCCTCAGAAATGTAAATGAAGAAAATATCTAA
2001 TAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' guccggcccUGUUCACGUUau 5'
                   || |||||||  
Target 5' aacaacaaaAC-AGTGCAAga 3'
 904 - 923 127.00 -7.10
2 
miRNA  3' gucCGGCCCUGUUCACGUUAu 5'
             ||: | |:|  |||||| 
Target 5' cttGCTAGTATA--TGCAATa 3'
 1607 - 1625 125.00 -7.30
3 
miRNA  3' guccGGCCCUGUUCACGUUau 5'
              ||| ||:||| ||||  
Target 5' gactCCGTGATAAGAGCAAgg 3'
 35 - 55 121.00 -14.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1 HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
24 mmu-miR-92a-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT002971 Hipk3 homeodomain interacting protein kinase 3 2 1
MIRT002978 Mylip myosin regulatory light chain interacting protein 3 1
MIRT004280 Trp63 transformation related protein 63 4 1
MIRT007244 Map2k4 mitogen-activated protein kinase kinase 4 1 1
MIRT053813 Zfpm2 zinc finger protein, multitype 2 3 1
MIRT054699 Tbx3 T-box 3 3 2
MIRT054792 Shox2 short stature homeobox 2 1 1
MIRT578185 Ncam2 neural cell adhesion molecule 2 1 1
MIRT580680 Stk10 serine/threonine kinase 10 1 3
MIRT583677 Fam136a family with sequence similarity 136, member A 1 1
MIRT584193 Cpeb1 cytoplasmic polyadenylation element binding protein 1 1 1
MIRT587067 Gm5148 predicted gene 5148 1 2
MIRT589036 Rhbdl3 rhomboid, veinlet-like 3 (Drosophila) 1 1
MIRT595174 Zpbp zona pellucida binding protein 1 1
MIRT595805 Chm choroidermia (RAB escort protein 1) 1 1
MIRT595903 Cd69 CD69 antigen 1 1
MIRT596292 Tagap1 T cell activation GTPase activating protein 1 1 1
MIRT596301 Tagap T cell activation Rho GTPase activating protein 1 1
MIRT596475 Btg2 B cell translocation gene 2, anti-proliferative 1 1
MIRT598598 Il5ra interleukin 5 receptor, alpha 1 1
MIRT598840 Gm14420 zinc finger protein 970 1 1
MIRT601409 Uba1y ubiquitin-activating enzyme, Chr Y 1 1
MIRT606063 Zfp300 zinc finger protein 300 1 1
MIRT735310 PTEN phosphatase and tensin homolog 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-92a Vorinostat (SAHA) approved 5311 Microarray A549 human non-small cell lung cancer cells 19513533 2009 down-regulated
miR-92a 5-Fluorouracil approved 3385 Microarray HCT-116 colon cancer cell line 19956872 2010 down-regulated
miR-92a 5-Fluorouracil approved 3385 Microarray HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-92a 5-Fluorouracil approved 3385 Quantitative real-time PCR HCT-116 colon cancer cell line 19956872 2010 down-regulated
miR-92a 5-Fluorouracil approved 3385 Quantitative real-time PCR HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-92a Oxaliplatin (L-OHP) approved 5310940 Microarray HCT-116 colon cancer cell line 19956872 2010 down-regulated
miR-92a Oxaliplatin (L-OHP) approved 5310940 Quantitative real-time PCR HCT-8 colon cancer cell line 19956872 2010 down-regulated
miR-92a 3,3'-diindolylmethane (BR-DIM) NULL 3071 Quantitative real-time PCR Prostatic cancer 22412975 2012 down-regulated
miR-92a Isoflavone NULL 72304 Quantitative real-time PCR Prostatic cancer 22412975 2012 down-regulated
miR-92a Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse liver 19270793 2009 down-regulated
miR-92a Comfrey NULL 6440495 Microarray rat liver 21370286 2011 down-regulated
miR-92a Comfrey NULL 6440495 Quantitative real-time PCR rat liver 21370286 2011 down-regulated
miR-92a Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 down-regulated
miR-92a Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated

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