miRTarBase - #MIRT599076


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pre-miRNA Information
pre-miRNA mmu-mir-205   
Genomic Coordinates chr1: 193507463 - 193507530
Synonyms Mirn205, mmu-mir-205, Mir205
Description Mus musculus miR-205 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-205-5p
Sequence 7| UCCUUCAUUCCACCGGAGUCUG |28
Evidence Experimental
Experiments Cloned
Putative Targets

Biomarker Information
Biomarker ID Name Type Discovered From Mode Level Source Testing Methods
B4QZ0E miR-205 Safety Biomarker (SAF) Clinical/Experimental Data Expression Increase Serum Quantitative reverse transcription PCR
Gene Information
Gene Symbol Eif2ak2   
Synonyms 2310047A08Rik, 4732414G15Rik, AI467567, AI747578, Pkr, Prkr, Tik
Description eukaryotic translation initiation factor 2-alpha kinase 2
Transcript NM_011163   
Expression
Putative miRNA Targets on Eif2ak2
3'UTR of Eif2ak2
(miRNA target sites are highlighted)		 
>Eif2ak2|NM_011163|3'UTR
   1 GGCCTTTCTGAGAAAACATTCCTCTGCCGTGGTTTTCCTTTAACGATCTGCAGTCTGAGGGGAGTATCAGTGAATATTAT
  81 CCTTCTTTTCTTAATACCACTCTCCCAGACAGGTTTTGGTTAGGGTGACCCACAGACATTGTATTTATTAGGCTATGAAA
 161 AAGTATGCCCATTTCCTCAATTGTTAATTGCTGGGCCTGTGGCTGGCTAGCTAGCCAAATATGTAAATGCTTGTTTCTCG
 241 TCTGCCCAAAGAGAAAGGCAGGCTCCTGTGTGGGAAGTCACAGAGCCCCCAAAGCCAACTGGATGAGGAAGGACTCTGGC
 321 TTTTGGCATAAAAAAGAGCTGGTAGTCAGAGCTGGGGCAGAAGGTCCTGCAGACAGACAGACAGACAGACAGACAGACAG
 401 ACAGACAGACAGAGACACAAAGACATGGACTAGAATGGAGGAGGGAGGGAGGAAGGGAGGGAGGGAGAGAGAGAGAGAGA
 481 AAGAAAGAGAGAGAGACCACATGGAGAGACAAAATGGCTTAAGTTAGCTGGGCTACCTGAGAGACTGTCCCAGAAAACAG
 561 GCCAACAACCTTCCTTATGCTATATAGATGTCTCAGTGTCTTTATCATTAAACACCAAGCAGGACTGCTAAAAACTCTGC
 641 AATAGGGTTTTTTTTTTCCTGTTACTTCAAAAGCAATCTTACAAAGTTATTTTTTTGACAATTCCATACATGCATTGTGT
 721 TCTGATCCCACTCTGAACCCTCTGCCATTCATGCCTTGTCTGTCATGTGAACTGTTGCCTCTGAATGTGGGGGTCCAAAT
 801 TAACCCTCTGCCCTTGAGTGGCTTCTCTCAGGTAGTGATTGTGATGAGAAAAGTAATGAGATGCTGGCAAAGATGTGCAG
 881 AAAGAAGAACACTTCTCCACTGCTGGTAGGATTGCAAGCTGGTACAACCACCCTGGAAATCAGACTGGAGGTTCCTCAGA
 961 AACACAGTACTACCTGAGGACCCAACAATACCACTACTGGTCATATACCCAGAAGATGGTCCAACATGTAATATGGACAC
1041 ATGCGCCACTATGTTCATAGTAGCCTTATTTATAATAGCCAGGAGCTGGAAAGAACCCAGATGTCCCTCAGCAGAGGAAT
1121 GGATACAGAAAATGTGGCACATTTACACAATGGAGTACTACTCAGCTATTAAAAATGAATTCATGAAATTCTTAGACAAA
1201 TGGATGGATCTGGAGGATATCATCTTGAGTGAGGTAACCCAATCGCAAAAGAACACACATGATATGCACTCACTGATAAG
1281 TGGATATTAGCCCAAAAGCTCCAAATAACCAAGATACAATTCACAGACTACATGAAGCTCAAGAAGAAGGAAGACCAAAG
1361 TGTGGGTGCTTTGGTCCCTCTTAGAAGGGGAACAAAGTACTCACAGGAGCAAATATGGAGATAGAGTGTAGAGCAGAGAC
1441 TGAAGGAAAGGCCATCCAGAGACTGTCCCATATACAGAGACTGGGAATTCATCCCATACACAGTTACCAAACCCAGACAC
1521 TATTGTGGATGCCAAGACATTCATGCTGACAGGAGCCTGATATGGCTGTCTCCTGAGAGGTCCTGCCAGAGCCTTACAAT
1601 ACAGAGACTGATGCTCACAGCCAACCACTGGACTGAGTGTGGGGTCCCCAATAGAGGAGTTAGAGAAAGGACTGAAGGAG
1681 TTGAAGGGGTTTGCAACCCCATAAGAACAACAATATCAACCAAGCAGACCCCCCAGAGCTCCCAGTGACTAAGCCATCAA
1761 CCAAGGAGTACACATGGCTCCAGCTGCATATGTAGCAGAGGATGGCCTTGTCATGTATCAAAAGGAGGAGAGGTCCTTGG
1841 TCCTATGAAGGTGCGATAGATGCCCCAGTATAGGGGAATCAAGGGCAGATAGGTGGGTTGGAGGAACACCCTCATAGAAG
1921 CAGGGGGAGTAAGGAAGGATATGGGGATTTCTGGGAGGGGTGGAAACTAGGAAAGGGGGTAACATTTGAAATGTAAATAA
2001 AGAAAATATCCAATTAAAAAAAAAAGAAAAAGAAAAAAGAAAAGAATAGTAATAAAATGGTACAGGAAGTAGAGTTATAT
2081 TGCAATAAACCTACTGTTGGGCTTTCAGGACTGGTTTGTGGGAGGAATGTGAAAAAGTTTGAAGCCCCAGGTTAGAGAAG
2161 TCCTCAAATGGTATACGTCAAACTTACTGTGGTAGCTCAAAAGTCTCCTGAGAGGCCCTGCTTGGAGTTAGCCTTGTAGA
2241 GGTCCAGTCTTTCCTTGTTGTTCTTTCAGACTTGCTTTGTAGAATATTGGTAGTTACTTTGTGCCTTTGTATGCTGTAAT
2321 AGTTGTTTTATAGGGCCTCACAGCTAAGAGTTTCTCGCTGCTTCTCAAAGCACTTTGGACCTTTGCATGGAGTTGAGTAT
2401 TAAGATTATGGGAATTTCTGAGGTGGGACTGAAAGCATTTTGCATTATGAGATGGCCATGAGCCAACAGAGACTTGGACA
2481 CACTCCTCCACTGTCAACCGAGGCTTCTGCCAAATCTTCCCTGTCATGAAGGATTGTATCATCTGAAATTGAGTCTAAAT
2561 AGATAAATAAATAAGTAAATAAATCTCTCAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' gucuGAGGCCACCUUACUUCCu 5'
              ||:|  ||  ||||||| 
Target 5' aaatCTTCCCTGTCATGAAGGa 3'
 2512 - 2533 154.00 -13.90
2 
miRNA  3' guCUGAGGCCACCUUACUUCCu 5'
            | | ::|||   ||||||| 
Target 5' agGTCCTTGGTCCTATGAAGGt 3'
 1831 - 1852 152.00 -15.80
3 
miRNA  3' guCUGAGGCC--ACCUUACUUCCu 5'
            |||  :||  | |||||:||| 
Target 5' aaGAC-ATGGACTAGAATGGAGGa 3'
 420 - 442 141.00 -19.30
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1 HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
24 mmu-miR-205-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT003192 Pten phosphatase and tensin homolog 4 1
MIRT007192 Lrrk2 leucine-rich repeat kinase 2 3 1
MIRT013552 Dok4 docking protein 4 1 1
MIRT013553 Atp1a1 ATPase, Na+/K+ transporting, alpha 1 polypeptide 1 1
MIRT054551 Gsk3b glycogen synthase kinase 3 beta 1 1
MIRT578673 Gramd1c GRAM domain containing 1C 1 1
MIRT580923 Slc35a1 solute carrier family 35 (CMP-sialic acid transporter), member 1 1 1
MIRT581415 Rbm47 RNA binding motif protein 47 1 1
MIRT582912 Il1r1 interleukin 1 receptor, type I 1 1
MIRT584261 Clasp1 CLIP associating protein 1 1 3
MIRT584588 Bcl6 B cell leukemia/lymphoma 6 1 1
MIRT587459 Decr2 2-4-dienoyl-Coenzyme A reductase 2, peroxisomal 1 1
MIRT588441 Yme1l1 YME1-like 1 (S. cerevisiae) 1 1
MIRT591352 Fgf5 fibroblast growth factor 5 1 2
MIRT595045 Cacnb4 calcium channel, voltage-dependent, beta 4 subunit 1 1
MIRT595313 Gpr35 G protein-coupled receptor 35 1 1
MIRT596159 Olfr287 olfactory receptor 287 1 1
MIRT596973 Tmem132b transmembrane protein 132B 1 1
MIRT597582 Ptprn2 protein tyrosine phosphatase, receptor type, N polypeptide 2 1 1
MIRT599076 Eif2ak2 eukaryotic translation initiation factor 2-alpha kinase 2 1 1
MIRT600592 Nfib nuclear factor I/B 1 1
MIRT602263 Gm4944 zinc finger protein 994 1 1
MIRT604828 Mylip myosin regulatory light chain interacting protein 1 2
MIRT604972 Gas2l3 growth arrest-specific 2 like 3 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-205 N-ethyl-N-nitrosourea NULL 12967 Quantitative real-time PCR mouse liver 21029445 2010 up-regulated
miR-205 Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated
miR-205 Benzene NULL 241 MiRNA PCR array white blood cell 24780745 2014 up-regulated
miR-205 Benzene NULL 241 MiRNA PCR array blood mononuclear cells 24780745 2014 up-regulated
miR-205 Hydroxamic acid HDACi LAQ824 NULL NULL Microarray breast cancer cell line SKBr3 16452179 2006 down-regulated
miR-205 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 down-regulated
miR-205 Enoxacin approved 3229 Quantitative real-time PCR HCT-116 and RKO colon cancer cell lines 21368194 2011 up-regulated
miR-205 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 up-regulated
miR-205 Acarbose Approved 444254 Microarray diabetic rats cells 24260283 2014 up-regulated
miR-205 Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 down-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-205-5p Cisplatin 5460033 NSC119875 approved sensitive Low Prostate Cancer tissue and cell line (DU-145, PC-3)
mmu-miR-205-5p Gemcitabine 60750 NSC613327 approved sensitive Low Pancreatic Cancer tissue and cell line (MIA-PaCa-2, CAPAN-1)

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