miRTarBase - #MIRT599554


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miRNA Infomation
miRNA namemmu-miR-1194

Gene Information
Gene Symbol C3ar1   
Synonyms AZ3B, C3AR, HNFAG09
Description complement component 3a receptor 1
Transcript NM_009779   
Expression
Putative miRNA Targets on C3ar1
3'UTR of C3ar1
(miRNA target sites are highlighted)		 
>C3ar1|NM_009779|3'UTR
   1 AGATGTGGCCCTGGGAACCTAAGCAGAGTTCTCAGGTGAACAGTGATGGATGACATGTGAGCAGGACACTTTAGACAATT
  81 TGGCGACTCTCAGAGAAAGGTCTCTTATTGACATCAGCATCATTTGAAAACATTAAAGATGCAAAATTTCAAGCCCCATC
 161 CCAGATGTGTTGACTCAGAATCTCTGGCCCATGGGACCAGTGTTTTAACAGGCCTTCTTGTTTCCATCAGTGTTAAGTTT
 241 TACCTCATTTGGCTTAGTCTATTCCCATCCCTGACTACACCATGTGCAATGAATAACTTTTTCATCTGTTTTCAGTATTC
 321 TTTTTTTTTCCTTAGCATCATCTAAACTTCTAGTTTGCATGGAAGGCTGCTCTTATTGTTCTGAATGGAAGATATTCATT
 401 TATTGTACAGTTTTGTGGTGGTGACAAGTGATTTTTAAGTGGGGAAAGAGACACAGTAAGAAAAGATCTATGAAAGCAGG
 481 GAGTGTTGAGTTAGAGTTTGACAGAACACAGTGCCAAATGCCACCCACTAAAAGCAACCTGAGATAATTCCAGTGTTCAT
 561 GTGAGCAAGTGAGCACAGATACACATAAACACTTTCCTACTCCTGGAGTGTTTTAGAAGTTGTAGCTTGGAGCTCTCTCT
 641 TCCCTGTGTATTCTGCTTGTTCATAGAGCCATCTATGTTCCATTTCTTTTTGTTTGTTGGTTTGGCTTCTTGTGTTGGTT
 721 GGTTGGTTGGTTGGTTGGTTGGTTGGTTGGTTGGTTGCTTGGTTGGTTGGTTGGTTGGTTGGTTGGTTGCTTGGTTGCTT
 801 GGTTTACTGTTTTTTCAAGACAGTAGCCCTGGCTGTCCTGAAACTCATTCTGTAGACCAAACTGGCCTGAAACTCAGAGA
 881 TCAGTGTGCCTCTGCCTCCAAGGAGTCTTCTTAAGGTCTTGAATTCCTTTCTGTTTTCCACACAGGGCCTTGCCATCTCC
 961 TGCTGGGATTAGGCGTGTGCAGGTGTGCAGTACGTACCTGGCCCCATCGTCTTCGGCTTTTTACATTAGACTTTGTATCT
1041 CACATAGAATAAAACAATATCCTAATGGGATGATGGGTGGAACTTCAGGATCAATGCATGCTTCAACAGCAATATCAAAA
1121 GATTTTAAACATGAGTCTAGGTATCTTACATGACAAAAGTGTCAGGCACACTCTCTTTTTTTTCTTTTCTTTTCTTTTCT
1201 TTTTTTTTTTTTCAAATTAATGTTTGCCTGGACCATGCTAATATAAAGTACTGTATGCAGCCAATTTTAAAGGAACTAAT
1281 TAAAAATGAAATAAAAAAGGGTGGTTTCTCCAGTGCCCAGTAGTTCCCATCTCTCCCTACTTTGCAAGAATTGTAGCTGT
1361 TCCTTCTGATATTTACCATGGTGTGCCTAAAACACCTCTTATGCTGCTGATCTTTGTAGACAGGAAGAAAAGGCTGTCTT
1441 GATTTTGATTTACTTTACCTACATTTTCATCCAGCACCCCAGTGTTGACTGGTATCACACATTAATCTGTTCGTGATCAT
1521 TTTTCTCTTCCCTTTGTTGTCGGGTTTTGCTTGCCTTCGTGCCATCATCTCAGTGTAGCACACACAATCTAGCTTCAGAC
1601 TCCCAGGTCTCTTGTTATAGTAACCAGATGAGCACCACCACCATCCCCACCCCCAGCTGACCACACAATTTTCCTGTCAA
1681 TACATTTCAATAGCATACAGCTTTTCTCTGTCCTCTCATTTTTCATGCTGACATATATTAATTGTACATATTAATAAGGT
1761 TCCACGTGGCTGATCCATCCATGCACACAACATTCACACTTTCACCCCCAGCCTCTTCTTTATCATTATTCTACTTACTG
1841 ATTGACTTTAAAAAAAAAAAAAAAAGTCTGAAGAATCTCATGCTGGCCTTAAAATCACTATGCAACCAAAAATGAGTTTG
1921 AAATTCAAATCCTCCTGCCTCCCATGGGCTGTGATTATAGGCATGGAACACTATGCCCACTCTTATGTGGTACTGGGACT
2001 CAAAACCAGGGCTTCATGCATAGTCGTTAAGCATCCTACCAACTAAACTACTCTGCACCCGCCCCCCAGCCTGCTCAGGT
2081 TGACATTTCTGGCCCTCATATATGAGACTATGTGATACGTATGTGTCCATTCACATCCATTCTGTTAAAACCAGTATTTC
2161 ACTCATTGTTATGGCTGAATAACACTGCACTGTGTATGTGCATGCACACGCATGTGTGTACATGTGTATGTGCACGTGTG
2241 TGTAGTAAGTGCCATTATCCACCCAACTACCAACCAATGCCTAAGCAGATTCTATATCTTAGCTATTATACATAGTACTA
2321 CACTCAACCTTTAGTTTAAAAAAAGAAAACCAGCAGCTCCCATAATAATCTATTATGTGGATACTTTCCATGTGTTAAAT
2401 CACTAGAAAGTCAAGCTTGTTTGTACAGATAGGTATGTCTAAGATGAACGAGTTAATCTACAAAGACGGAAGATGGAATG
2481 TAGTTTAGTCTTGTGATACATTATTTATTCAGTTGTCAAGAGAAGGGCCTTAGGCTACAACTGTTGTATGAACAATTATG
2561 TGGAAATTTCAAATCACTAAAAGATCTTGTTTACTACGAGGTAATTCTGGGCATAAGACTGCAGTTCTGCCTTTCCAGTA
2641 TGAGCGGTTTACATATTCCCTATGAATAAATGCTTTTATCTAC
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1 HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
CLIP-seq Support 1 for dataset GSM4751761
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 2
Location of target site NM_009779 | 3UTR | CUGAAACUCAUUCUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM4751762
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 3
Location of target site NM_009779 | 3UTR | CUGAAACUCAUUCUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM622570
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1A
Location of target site NM_009779 | 3UTR | AGCCCUGGCUGUCCUGAAACUC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM622571
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1B
Location of target site NM_009779 | 3UTR | AGCCCUGGCUGUCCUGAAACU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 5 for dataset GSM622572
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT2
Location of target site NM_009779 | 3UTR | AGCCCUGGCUGUCCUGAAACUCAUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 6 for dataset GSM622573
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / KO1
Location of target site NM_009779 | 3UTR | UAGCCCUGGCUGUCCUGAAAC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 7 for dataset GSM622574
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / KO2
Location of target site NM_009779 | 3UTR | AGUAGCCCUGGCUGUCCUGAAACUCAUU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
39 mmu-miR-1194 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577399 Usp29 ubiquitin specific peptidase 29 2 2
MIRT578645 Gtf2h2 general transcription factor II H, polypeptide 2 2 4
MIRT583791 Elf1 E74-like factor 1 2 2
MIRT585587 Tox4 TOX high mobility group box family member 4 2 4
MIRT585769 Spg21 SPG21, maspardin 2 2
MIRT586369 Pctp phosphatidylcholine transfer protein 2 6
MIRT586934 H2-T24 histocompatibility 2, T region locus 24 2 4
MIRT587278 Fam154b stabilizer of axonemal microtubules 2 2 2
MIRT589358 Osbpl8 oxysterol binding protein-like 8 2 4
MIRT591418 Cd300a CD300A molecule 2 4
MIRT594881 Lpar6 lysophosphatidic acid receptor 6 2 2
MIRT594899 Hs6st2 heparan sulfate 6-O-sulfotransferase 2 2 2
MIRT595087 Unc5d unc-5 netrin receptor D 2 2
MIRT595608 Jazf1 JAZF zinc finger 1 2 2
MIRT595914 Syne2 spectrin repeat containing, nuclear envelope 2 2 2
MIRT596713 Ybey ybeY metallopeptidase 2 2
MIRT597013 Thoc6 THO complex 6 2 2
MIRT597522 Rbak RB-associated KRAB zinc finger 2 2
MIRT598050 Nip7 NIP7, nucleolar pre-rRNA processing protein 2 2
MIRT598082 Neu3 neuraminidase 3 2 2
MIRT598528 Itsn1 intersectin 1 (SH3 domain protein 1A) 2 2
MIRT598953 Ggps1 geranylgeranyl diphosphate synthase 1 2 2
MIRT598967 Gata5 GATA binding protein 5 2 2
MIRT599086 Efcab4a calcium release activated channel regulator 2B 2 2
MIRT599348 Commd7 COMM domain containing 7 2 2
MIRT599554 C3ar1 complement component 3a receptor 1 2 2
MIRT600166 Xpo7 exportin 7 2 2
MIRT600668 March5 membrane-associated ring finger (C3HC4) 5 2 2
MIRT600971 Dlc1 deleted in liver cancer 1 2 2
MIRT601455 Tnfsf10 tumor necrosis factor (ligand) superfamily, member 10 2 2
MIRT601502 Tmem88b transmembrane protein 88B 2 2
MIRT601514 Tmem151a transmembrane protein 151A 2 2
MIRT601558 T2 brachyury 2 2 2
MIRT601925 Onecut3 one cut domain, family member 3 2 2
MIRT601984 Mzt1 mitotic spindle organizing protein 1 2 2
MIRT602669 Car5b carbonic anhydrase 5b, mitochondrial 2 2
MIRT603331 Slc35d2 solute carrier family 35, member D2 2 2
MIRT605546 Ppp1r16b protein phosphatase 1, regulatory (inhibitor) subunit 16B 2 2
MIRT606291 Nrcam neuronal cell adhesion molecule 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-1 Anthocyanin NULL 145858 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Caffeic acid NULL 689043 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Catechin approved 9064 Microarray apoE−/− mice 22253797 2012 up-regulated
miR-1 Curcumin NULL 969516 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Ferulic acid NULL 445858 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Hesperidin NULL 10621 Microarray apoE−/− mice 22253797 2012 up-regulated
miR-1 Quercetin NULL 5280343 Microarray apoE−/− mice 22253797 2012 down-regulated
miR-1 Galactose NULL 6036 Quantitative real-time PCR lens 22736950 2012 up-regulated
miR-1 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miR-1 Trichostatin A (TSA) NULL 444732 Quantitative real-time PCR myocardial differentiation of mouse ES cells 19521018 2009 down-regulated
miR-1 Sulfonyl-hydrazone-1 (SHZ) NULL NULL Quantitative real-time PCR Murine broblast-derived Induced pluripotent stem cells 21445862 2011 up-regulated
miR-1 Cocaine NULL 446220 Next-generation sequencing ventral striatum 21708909 2011 up-regulated
miR-1 Atorvastatin approved 60823 Quantitative real-time PCR Cardiomyocyte 23860036 2013 down-regualted
miR-1 Glucose NULL 5793 Quantitative real-time PCR endothelial cells 24394957 2014 down-regulated
miR-1 Docosahexaenoic acid NULL 445580 Quantitative real-time PCR Caco-2 cells 24623846 2014 up-regulated
miR-1 Palmitic acid approved 985 Quantitative real-time PCR Caco-2 cells 24623846 2014 up-regulated
miR-1 17beta-estradiol (E2) approved 5757 Microarray MCF-7AKT breast cancer cells 19528081 2009 down-regulated
miR-1 Essential amino acids (EAA) NULL NULL Quantitative real-time PCR skeletal muscle of young adults 19828686 2009 up-regulated
miR-1 Hydrogen peroxide (H2O2) NULL 784 Quantitative real-time PCR Human umbilical vein endothelial cells 21527937 2011 down-regulated
miR-1 Trichostatin A (TSA) NULL 444732 Microarray apoptosis-resistant breast cancer cells 21971930 2011 up-regulated
miR-1 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-1 5-Fluorouracil approved 3385 Microarray CNE cells 22614822 2012 up-regulated
miR-1 Bicalutamide approved 2375 Microarray prostate 22674191 2012 up-regulated
miR-1 Arsenic trioxide approved 14888 Quantitative real-time PCR cardia 22889704 2012 up-regulated
miR-1 Perfluorooctane sulfonate NULL 74483 Microarray zebrafish embryos 20878907 2011 up-regulated
miR-1 Quinidine approved 441074 Quantitative real-time PCR myocardial infarction (MI) rats 19775284 2009 up-regulated
miR-1 Tanshinone IIA NULL 164676 Quantitative real-time PCR myocardial infarction (MI) rats 19775284 2009 down-regulated
miR-1 Tanshinone IIA NULL 164676 Quantitative real-time PCR post-infarction rat cardiomyocytes 21220930 2011 down-regulated
miR-1 Isoproterenol approved 3779 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-1 Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 up-regulated
miR-1 Thiourea (TU) NULL 737139 Quantitative real-time PCR skeletal muscle and heart 22142802 2012 up-regulated
miR-1 Thiourea (TU) NULL 737139 Quantitative real-time PCR skeletal muscle and heart 22142802 2012 down-regulated
miR-1 Isoproterenol approved 3779 Quantitative real-time PCR heart 22847192 2012 up-regulated

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