miRTarBase - #MIRT599752


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pre-miRNA Information
pre-miRNA mmu-mir-470   
Genomic Coordinates chrX: 66813951 - 66814025
Synonyms Mirn470, mmu-mir-470, Mir470
Description Mus musculus miR-470 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-470-5p
Sequence 9| UUCUUGGACUGGCACUGGUGAGU |31
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Amy2a4   
Synonyms mAmy2-3
Description amylase 2a4
Transcript NM_001160150   
Expression
Putative miRNA Targets on Amy2a4
3'UTR of Amy2a4
(miRNA target sites are highlighted)		 
>Amy2a4|NM_001160150|3'UTR
   1 GAATCTATATTAAAGAGATTTGGATTAAGCATCAATTGCATGTGTTTCTTTTTCATATGCTCTGTACTATATTTTTATTT
  81 CTATTTTGTTTCTTTATTTTTATGTTTCCATGTGTGCTTTGTCTTCTGAGCCCTGGAGTGTATGTAGGATGTTTGAACAA
 161 CCCTGTGCTGTGGCATAACCTCTCTACATTTTCCCATGAGCTGTGCTGATCAAACTAAATCCATGAGGCTTCCCTGGAAC
 241 TCACTTTTATCTGCTATTCTATGTTGTTATCTCATATGCCCTTTAATTCTTGCAGTTTATTTCCTTGTAGTAAAAACCAT
 321 AGACAACTGTCTTAATTAGGGTTTCTATTCCTGCACAAACATCATGACCAAGAAGCAAGTTGGGAAGGAAAGGGTTTATT
 401 CAGCTTAAACTTCTATACTGCTGTTCATCACCAAGGAAGTCAGGACTGGAACTCAAGCAGGTCAGGAAGCAGGAGCTGAT
 481 GCAAGGCCATGGAGTGATGTTCTTTACTGGCTTGTCTCCTCTGGCTTGCTCAGCCTGTTCTCTTATAGAACCAAGACTAT
 561 CAGCTCAGAGATGGCACCACCCACAAAGGAACCTCCCTGCTTGATCACTAATTGAGAAAATGCATTGCAGCTGGATCTCA
 641 TGGAGGCATTTCCCCAATTGAATCTCTTTTCTCTGAGATAACTCCAGCCTGTGTCAAGTTGACACAAAACTAGCCAGTAC
 721 AACAATAAACTTGAATAGGCAAATATCTTAAAACTCACATAAAATCATCTGATAACAACATCCTAAATATCCTAAGCATT
 801 AGCAGAATGTCTAAATGTTTTTTTCTAATAAATTCTCAAATATCTTAATAGTGTTCTGGCAAATATTTTGCTTTACTATT
 881 ACTATAAATTCTTTCATGTAGCCGTTTTAAAGTATGAAAATTTATTTTCAATAAATTTCATTGACAATCTAATTACTCAT
 961 TAAACATGCTTTTGGCTACTTGGAAAATCCATCATTGAATAATTTCTTCTCATGACTGTTTTCTTCAAGTTCCTTTCATA
1041 ATTAATTTTTCCAGGAAAGAAGTTTCCTCATTCCACAATACCCACCTGTTTCTTTTTTGGTGTTCTGTTATGCATGGCCT
1121 GTGGTTCTTGTGATCGGGGAGACCCTTGTTCAGTAGAATGTCTCTGCTGGCGATTTGGCGGCTTGTGTGACCGCTGATGT
1201 TTAGGGTACTGGGGTTTTAGGAACTGGCATAACCTTGGATTCTGGATAAGTCATTGTCTCTGGTAGAGCAAAGAGCTTCT
1281 GCCAGAGTTGTGGTATGGGACATGGAGATGAGTGAAGGGGCCACTGTTGGGGAAAGTTCCACAGGCTCTGAGGAAATGTT
1361 GGTGGTCTGCCTCAGGGCCTGACCTGATAGAGCAGGGTGATTCTGCTGGAGCTATGGGTCAAGATATGGAGACCTGGAAG
1441 GCATGCACTTGTACCTGCTGTGCTATGTTGCTGGCTCATCTGTACTTTGATTTTTAAAGTTTATTTATTTTTTACCAATA
1521 GCCTTAAACAATGAACTTGAATAGGTAGACATGATGAATTGCACATGTAATCATTAGATAACTTCATCCCAAATTTACTA
1601 TGCATTAGGAGAAAATCTAAATTTTTTTTCGAAGAAAATAATCAGAACTTTGGATGGCTTCCCAAGAAATATCTTTTTAC
1681 CATTAATATGATTTCCTTCATGTTCCCCTATATATTTTATGAAAAAGTGTTTCAATATAAATTCACTCACTTATTAAACA
1761 TGCTTTGGTTACTTTAAATCTCATCATTGCTCAATTTCCTCTGGTGGCCATTTTTTAAAAAAAATTGTCTTATAAATAGT
1841 TATTTCAAGAAAAAAATATCTTTTTTTAATTCCACAATACTAACAATACTGACAGCACATATTGTAACCTATCTTGTTAC
1921 TCAGTTAATGGCATTCTTTGTTTGGTGTACAGAAGCATTTTAGTGTCATGAATTCCTGTTCTTATTGTATGAGAAATTGC
2001 AATCACATTCAGTAAAGCCTTCCTTTTGCAAATGAGTTTAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugAGUGGUCA-CGGUCAGGUUCUu 5'
            |:|: | | |::| |:||||| 
Target 5' tcTTATAAATAGTTATTTCAAGAa 3'
 1828 - 1851 141.00 -11.70
2 
miRNA  3' ugAGUGGUCA-CGGUCAGGUUCUu 5'
            |||: | |  ::  ||||:|| 
Target 5' ttTCATAATTAATTTTTCCAGGAa 3'
 1034 - 1057 129.00 -10.61
3 
miRNA  3' ugaGUGGUCACGGU-CAGGUUCUu 5'
             ||| |   :|| | |||||| 
Target 5' ctgCACAAACATCATGACCAAGAa 3'
 351 - 374 124.00 -12.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ugaguggucacggucagGUUCuu 5'
                           ||||  
Target 5' ------------agaagCAAGuu 3'
 		1 - 11
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
26 mmu-miR-470-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT001002 Nanog Nanog homeobox 4 1
MIRT004344 Pou5f1 POU domain, class 5, transcription factor 1 4 1
MIRT005701 Ass1 argininosuccinate synthetase 1 2 1
MIRT579600 4930563E22Rik RIKEN cDNA 4930563E22 gene 1 3
MIRT579658 1810030O07Rik RIKEN cDNA 1810030O07 gene 1 2
MIRT587091 Gldn gliomedin 1 1
MIRT591592 Zbtb44 zinc finger and BTB domain containing 44 1 1
MIRT594490 Dcp2 decapping mRNA 2 1 1
MIRT596386 Wipf2 WAS/WASL interacting protein family, member 2 1 1
MIRT596756 Utp23 UTP23 small subunit processome component 1 1
MIRT597051 Terf2ip telomeric repeat binding factor 2, interacting protein 1 1
MIRT598179 Mrpl19 mitochondrial ribosomal protein L19 1 1
MIRT598583 Intu inturned planar cell polarity protein 1 1
MIRT599191 Ddx19b DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b 1 1
MIRT599360 Colec12 collectin sub-family member 12 1 1
MIRT599386 Cln8 ceroid-lipofuscinosis, neuronal 8 1 1
MIRT599752 Amy2a4 amylase 2a4 1 1
MIRT599756 Amy2a3 amylase 2a3 1 1
MIRT599760 Amy2a2 amylase 2a2 1 1
MIRT599869 Acnat1 acyl-coenzyme A amino acid N-acyltransferase 1 1 1
MIRT600012 2900026A02Rik RIKEN cDNA 2900026A02 gene 1 1
MIRT601650 Siglece sialic acid binding Ig-like lectin E 1 1
MIRT602197 Hmmr hyaluronan mediated motility receptor (RHAMM) 1 1
MIRT605471 Slc7a14 solute carrier family 7 (cationic amino acid transporter, y+ system), member 14 1 1
MIRT605570 Oxsm 3-oxoacyl-ACP synthase, mitochondrial 1 1
MIRT732694 BDNF brain derived neurotrophic factor 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-470 (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 up-regulated
miR-470 (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 up-regulated
miR-470 (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 up-regulated
miR-470 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 up-regulated

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