miRTarBase - #MIRT600185


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pre-miRNA Information
pre-miRNA mmu-mir-449c   
Genomic Coordinates chr13: 113035983 - 113036091
Description Mus musculus miR-449c stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-449c-5p
Sequence 26| AGGCAGUGCAUUGCUAGCUGG |46
Evidence Experimental
Experiments MPSS
Putative Targets

Gene Information
Gene Symbol Wipf2   
Synonyms 1110014J05Rik, 5730509C05Rik, AA407487, Gm1176, Wich, Wire
Description WAS/WASL interacting protein family, member 2
Transcript NM_197940   
Expression
Putative miRNA Targets on Wipf2
3'UTR of Wipf2
(miRNA target sites are highlighted)		 
>Wipf2|NM_197940|3'UTR
   1 AGCTTGGCGTAGTCCTGCTCCTCAGGAAAAGGATGGACCTCCTCTTCTCAGATGGCCTCCCAACCCTAATCCCTGCATGA
  81 GAGCTCCTGCAGTGTTTCTCCAGTGCAGCCAACCCAGGCTCTGAGCATCCTCCAGCCCAGCTCCATCTATGCATCTCCTC
 161 TCTGGACTCGGTGATTGGACTCTTTCTATTGGCAGTAGGGTCTACTCCCATCTCCACCTTCTTCCAGCAAGCCCTGCTAG
 241 CCAGAGGAGGAGAAAGCTTCCACGCCTCCTGCTTTCTTCCAGGGAAAGGTGCCTTGTTGTGGTGAATGGACTCACATTGG
 321 GCAGGGCAGAAAGTGTAACCCCTGTCTCTGTCATTCACCGAGGGCGAAGTGTGGTGTGTGCATTATAGCCCATAGTTTAG
 401 GAGGTTGGTGTGGCCAGAACTTCTGAGGAGAGGTGTTCCATGATGCAGGGAGGGGGTTTGCAGAGAAGTGATCTGTTTTA
 481 AAGGTCGGGTTACAGAAAGGACAAGGACATGGGTCTTCTTCATTTTCAAGTGTTTTGGTTTTGTTCTCATCCCCTTCCCC
 561 CATCCCAAGAGATGAGCAGTGCATACACACTAAGTACTGATCCGCTAACTAAACGGTAAAGGGCACGAAGCAACGGAGGG
 641 TCATTTCAGAGCCAGTCAGCTCTTGTGCAGCTAAGGGCCAGGGCTGTCCGAGCTCTGGGACCTCCTGCCCCTTCCCCAGG
 721 GTGCTAGGCTGAACTGTGTCCCTTCCCGATCCCACAAGAGAAGAGCCACTTTGTTGAGGGCATTGTCTATTCTCAAGTCA
 801 CAAACCCTACAGACTTCCTATAAGACATGGAAAGATAGGGCCTTAGCCACAGCCTCCTGATGGCTTCAGTTTTTTCTGAT
 881 TAACCCTGAAGTGGCCAGCAGCCTGAGTCCTTCAGCCCTTGGGGAAAGCTCCAGGGAGACTTAAGAAGGGAACCGAAGCC
 961 CTGGCTTCATGGGAGTGTCTGGCTCTTCAGGGCCAGTAAGGTCTTGGCAGCCTGCTCATAGCGAGACTCTCACACCCATC
1041 AGACGGCAGGCTCTTCCTGTTTTCTCTGCCACCCACCCATACCTTACTGAGACTGCAGGGTTTAAGGCCACAGCGTAGAA
1121 AAGGGAGCTTCAGGAGGAATACAGAGTCCTGTAAGCCAAGCACAAAGACATAGGGAGGCATGCCGCCCGCCATCTAGGAG
1201 GTGGGTCTTCCCTGGCGGCCAGCAATACATCGTTTCTCAGTCTTCCAAGGTGTAGGGAAACAGCACAGGAAGTGGGTGTT
1281 CCAACGGAGGCCGCTCTGTTGGCCAGGAAGTGTCTCTTCTCCACTAGCAGTGTGGGGCGCCTGAACTCCACACCCAGAGA
1361 AGTTGGTTTCTGACAGTCCTTGACCTGGGCACAAGCTGCTCAGATCTTACATCACGGTAGGGAGACCAGGCTGTTCTCTG
1441 AGTTGGGTAGGGGAAGCTAGTGAAAAACTTCCAGATACACCATGGGGTTAAATTATCGTAGGGGCTGTCCTTTTTTTTTT
1521 TATTATTATTATTATTTTTATTTTTATTCTCCGCCCACCCCACTCCACTCCCACCCCCTAAGACAGGGTTTCTCTGTGCA
1601 TTCCTGGCTGTCCTGGAACTTATTTTCTAGACCAGGCTGGCCTCAAACTCAGAGATCCCCTGTCTCTGCCTCCCAGGTGC
1681 TGGGACTAAAGGTGTGCGCCACCACGCTTGGCTCATAGGGACCTTTCTAATCTAGTGGACCATAGGCTAAGGATCTGTGT
1761 GTAATGCCAGGTCAGACCCAAATCTTCTAGGACTGGAGAAAGTGGACCTAACTTAGTTCATTTATTTATTGAGTGTTGCC
1841 CATTGCCAGCTCTTGGACCACTTGGACTTTTGGGACAGGCAGTGGTTAGATCTTACCGGACTTTTAATTGTTCACTATTT
1921 ATCTTCTTCTCAAATCTGTTAGCAGTTACGCTCCCAGCATCCTAACTTGAACAAAGGGAAGAGAGGGAAAAAAAGCTGAC
2001 ACTTGGAGAGTTCCAGGGTAGATTCTAATGGTTGAACTGTGAAGTCTTCCCATTTGTTGTTGAAATGGGAGCTGATGCCT
2081 TTTGTACAATGTTATCAAAGTGTGAAAATTTTCCCTTACTGAAGCATAGACCATCAGATATATGCGTGCGTATTTATGTA
2161 TGTGTATATATATATATATGTATATACACACACACATATAGATTTGTATACACACACATACATCTACTCACAAAGCCAAA
2241 CCCGGGCTTTTTGCTTGGGCTGATGGTACCTTCCTCGACTTGACCTGCCACCCTTTCTAATCCCCAGAATCAGACTGTGC
2321 CGCGTGGGTATTGATGATATATTTTCGTTGTCTTTGCCTGGTGGAACCACCACCAAAAAAAGGCTTGAATTTAGATCTAC
2401 ATGAGTGAGTTTGCTCCTCCAGTTAACGGATTGTGTTGATGACACAACCCCTAACTTAAATGTCTTTGCCTTATTCATCA
2481 TTTGGTCCTGCTGTTAATAACAAATTTATTATCATGTATATTTGCTATTGAAGATGGGAGTGTGATCCTCAAAATTGTTG
2561 GTCTGTTTTATATATTGTACACAGCTTGTAATGCAGGTTTAAGGTAGCCGGGCTGTGAAAACCCTGACACTGTTCCCCTT
2641 TAAACTGTCCTTGATGTCCTCATGGGAATGTCGTCTCTTCAGTGGAAGATTGGGTGGTCTCATGTTGAGGCTATTACTTA
2721 GTCCCTTTAATCCCCTTGTCCCCTCCCAAAGGGGAGAGACATTGCCCAGCTAAGCAGCCAGAAGCTCTGTCACAGCTTTC
2801 GTGGTATTTTTCCCTAGTGGGAAACGCTGGATGGCTGTTTCTTACTGCCCTGCCCTGTCAGGCGGTGGTTCTGATTTTCA
2881 AATGAGCTAGATGCTCCTCTTTCCCTTTCTCCAGTCACCAAACCTGGACCAAAGTACTTCATATTCCAGGTGTGGGTTCC
2961 TCTCTCCTGGGATTTGCTGCAGCTGCAGAGCCCTGCCCTTTTCGCAGCCTTGGCCACTTGTGTGAGCCACTAGGGCCATC
3041 CCAGCCTGATGGACGGGAGGCGGTCCTCACCAGGAATTCAATTCATTCCCTGCCCCCTTTCCTAGCCTAGGCAGAGAAAC
3121 TAGTCACTGGCCATTGCCCTGGGGGAATCCCCTCACGCAGTGGCTTCCCATCTTAGAACCCGGACTTACCTCCAGGCGGA
3201 GGTGAGGGGTGGGGAGTACTGTAAATAGACTTGCTGCAGAGCAACTCAGGGTTGGGGTATGTTTGAGTTCTCCTGATCAC
3281 TTGAAAACTAATCTGTAGGCTGAATGCTTCTGGAGTGTGGGGGAAATGTGACTCCGGGGTCCTTTGTCCTCTGAGGCTCT
3361 GCGGGTGGAGGGTAGGACTTCAGAGGCCCTTTGATAGCACGGCTGCGCTGTCTGCCCTTCTGGAAACCCAACCTGCCATC
3441 AGCTGTAAATCAGATTCTTCACATTCCAGGTGTAAGCTTTCCCTTCCCTGTGACTCTCAGTCCCTAGCTACATGGTCAGG
3521 GGGACTCTGTTAAACCACCCAAATTTAGGGAATGAGAGGGGAGAGAGGAGGCCATTAGGAGTCTGCCTTCAAGACTCATT
3601 TCTTGAAAACAAAATGGAAAAAAAAAAAGATAACCACCTTCAATCACAGGCAAAAACTAAACCAAGAAAAAAAAAAAAAA
3681 AGCAAACAAACCAAAAAACCCAACAGCAACCATGTAACTGAAGGAAGGGTCTGGTTCCGCTCCATCCCGTGTTCATTGTG
3761 CCTTTACTTGTGTTAGATCTCTGTGCTTTCTTCCTCCCCCTCTATGACGTCATTAATTAAAGCCTTCCTTGATAACCGCT
3841 GTTTCCTTTCTGCTCCTGTTTCTTACAGCTTGGTGGGTTTCTTCTCTGATTGTCTTCCATCTTACTCGCCTGGTGGCAGA
3921 GGGGCTTAGCGCACACACGCACCCTCTTTTTTTTTTTCTAACTTCTCCTTTCCCACAGTTTCCAGCCTGGAAGTTACAGT
4001 CAACACTGAGTAAGAGACCAGAGTGATTGTGTGCATGTGCGCGTGTGTGTGTCCATCTGCATGTGCGTCAGTTTCTTTGA
4081 AAAATAATTTATTGTGTGATTTCCTGTGGAGTTTTTGTTCAGGTTACAGTGCTGCTCCACAATAGCACGGATCCCTGTCC
4161 CCCTACCCCCGGCATAAAATGTATTATCTGGTCTCAGGTTGGATTCCTTGGTACGTTTCTTTCTGTGCAGTTTATTAAAC
4241 CTTAAAAGGAAAAAAACAAAAAGGAAAACCTCTGTGTATTCTTGTCGTGAGCAATACTGTTTCAAGTGACTGCAGGGAGA
4321 GAGGAGGAAACCCCATGGTCTCCTCTTCTGGACAAACGTGGCAGTTTGAGTTCAGGCTCCCTTAAGAGAGGACCTGTGGG
4401 TGGGTGTGGCAGAAACACTTGCAGCTTTCTGGGGCCTTTCTGCCTGTGCGTTTTATTTTTGTTTTTGTTTTTTGTTTGTT
4481 TGTTTGGTTGGTTGGTTTTTCAAGACAGGGTTTCTCTGTGTAGCTCTGGCTATCCTGGAACTCACTCTGTAGACCAGGCT
4561 GGCCTCGAACTCAGAAATCCGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCGTGCACCACCACTGCCCGGCTGCC
4641 TGTGCGTTTTTTAGAAGTCACCCTTTTGGCCTGGCCAACCAGTGATTCTGTTTTGTATTTTCTGCTTCATTCCCCTTGCC
4721 TTTCTCTCAAGGAGCCCCAGAGGCTCCTTTCCGTGGGGTCTGTTCCTTGTGACCCCTTAGACTCTGTCTTCCTGTGCCCG
4801 TGCTTCCAGGCCGATTCGCGCCAGACAGTGGAAGAAAAGGTGGGGGCAGGGCCCACATCTTGTGAAGCTCTGCATAGTCC
4881 TGCTGATCCTGGAACTCACTGTGTAGACCAGGATAGCCTTGAACTAGATCCTCTTGCTTCTTCCTGTGCAAGGTTTGGAC
4961 CACCACAACTGCCTTTTCAGCCGAGATCTTAGCCGGTTTTCTTGAGCTTTGACTTTAGGTGTTAAAATTCTACCAGGCCT
5041 CTTTGTTTCTGTGTTTGCCACTGATAGTAACTGCAGCTGAGTCCACTGAATTTGACTGCACTTTCACTGATAGCAGACCA
5121 GCCAGTTACTTCCCTCTGGGTTTATAATGGGTGTGTGTGTGTAGATAGCTCTCAAGGAATCTAGGTAGATATAATGGGGG
5201 TGTGTGTCTGTCTGTCTGTCTGTCTGTCTCTTGCTCTCAGGGAATCTAGATTAGGAAAACTTGCTGGGGAAAGGTTACTA
5281 CCAGAGTGGCTGAATTCCTGATTATTTGATCCATTATTGTTATTAATATGAATAATAAACTATTTATTGATTTCATTATT
5361 CTTTCTGACTTAATAGCCTGCATTATGGCATTTGCTTAAATGACCCCCAACTTCCAGCCCTGTAAATGACCTCACTCCGA
5441 TAGTATCGTTGGTCCTTGTGTGTACCTTTGCACATGCTCAAAGAAAGATGCCAGACAGGTCTTTCCAGTGTGACTTGATT
5521 CCTGCTCTGTGACCCCGCTTGAGTTAAGTGATAAGAACACCAGCTTGCTCACTGCTGATTGCTCAGGCACAGCTGTGTGT
5601 GCTGTGAATGTGTCTCGAGTATTTCCTGGCCCAGACCATGAACTGCAACTCGTGGGACGGAAGCTACCTTCCTCTTGCTT
5681 GTGAATTCTCCGCAGATGGTAAAATGCCAATCTCAGGATCATGACCACACCAGAGAAGAACTACAAGAAGGCTTTTTCAC
5761 CTTTGACACCACGGACGCCCAGTCTGATCTGTTTTTGCACATAATTAAATGTATTCTTCTACGTTTATGGCTTTGAGATT
5841 TAAAAGACTGTGTATGTGCTTTCCAACCATAAAAATCATTCCTGCACACAGTG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ggUCGAUC-GUUAC--GUGACGGa 5'
            | |||| || ||  ||:|||| 
Target 5' gaAACTAGTCACTGGCCATTGCCc 3'
 3116 - 3139 145.00 -15.40
2 
miRNA  3' ggUCGAUCGU--UACGUGACGGa 5'
            :||  |||  |  ||||||| 
Target 5' aaGGCGTGCACCA-CCACTGCCc 3'
 4612 - 4633 144.00 -14.40
3 
miRNA  3' ggUCGAUCGUUACGUGACGGa 5'
            |||| |||: || ||||| 
Target 5' gcAGCT-GCAGAGCCCTGCCc 3'
 2979 - 2998 138.00 -21.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ggUCGAUCGU--UACGUGACGGa 5'
            :||  |||  |  ||||||| 
Target 5' aaGGCGUGCACCA-CCACUGCCc 3'
 		15 - 36
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
41 mmu-miR-449c-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577806 Rnmtl1 mitochondrial rRNA methyltransferase 3 1 1
MIRT581641 Prex2 phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2 1 2
MIRT587396 Dnase2a deoxyribonuclease II alpha 1 1
MIRT588268 Smco1 single-pass membrane protein with coiled-coil domains 1 1 2
MIRT595436 9930013L23Rik cell migration inducing protein, hyaluronan binding 1 1
MIRT595972 Tbc1d2 TBC1 domain family, member 2 1 1
MIRT596032 Vps9d1 VPS9 domain containing 1 1 1
MIRT596042 Ubp1 upstream binding protein 1 1 1
MIRT596048 Shisa7 shisa family member 7 1 1
MIRT596070 Ywhag tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 1 1
MIRT596093 St6galnac3 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 3 1 1
MIRT596128 Rgs17 regulator of G-protein signaling 17 1 1
MIRT596176 Med22 mediator complex subunit 22 1 1
MIRT596199 Fyco1 FYVE and coiled-coil domain containing 1 1 1
MIRT597335 Sema4b sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B 1 1
MIRT598319 Mbd4 methyl-CpG binding domain protein 4 1 1
MIRT599424 Cercam cerebral endothelial cell adhesion molecule 1 1
MIRT599598 Bloc1s3 biogenesis of lysosomal organelles complex-1, subunit 3 1 1
MIRT599659 B3galtl beta-3-glucosyltransferase 1 1
MIRT600185 Wipf2 WAS/WASL interacting protein family, member 2 1 1
MIRT600239 Trim36 tripartite motif-containing 36 1 1
MIRT600385 Slc35e2 solute carrier family 35, member E2 1 1
MIRT600677 Mapre1 microtubule-associated protein, RP/EB family, member 1 1 1
MIRT601685 Sco1 SCO1 cytochrome c oxidase assembly protein 1 1
MIRT601718 Rheb Ras homolog enriched in brain 1 1
MIRT601800 Rab11b RAB11B, member RAS oncogene family 1 1
MIRT601868 Pin1 protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 1 1
MIRT602435 Epb4.2 erythrocyte membrane protein band 4.2 1 1
MIRT602459 Dusp11 dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) 1 1
MIRT602620 Cenpl centromere protein L 1 1
MIRT602637 Cd93 CD93 antigen 1 1
MIRT602799 Ankrd28 ankyrin repeat domain 28 1 1
MIRT603072 Zfp446 zinc finger protein 446 1 1
MIRT603468 Trmt10a tRNA methyltransferase 10A 1 1
MIRT604685 Sgk3 serum/glucocorticoid regulated kinase 3 1 1
MIRT605120 Cnnm3 cyclin M3 1 1
MIRT605335 Zfp317 zinc finger protein 317 1 1
MIRT606441 Fam118a family with sequence similarity 118, member A 1 1
MIRT606475 Ctsa cathepsin A 1 1
MIRT606636 Phc3 polyhomeotic-like 3 (Drosophila) 1 1
MIRT736224 TUBB4B tubulin beta 4B class IVb 3 0

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