pre-miRNA Information | |
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pre-miRNA | mmu-mir-1906-1 |
Genomic Coordinates | chr12: 109544541 - 109544620 |
Description | Mus musculus miR-1906-1 stem-loop |
Comment | None |
RNA Secondary Structure | |
pre-miRNA | mmu-mir-1906-2 |
Genomic Coordinates | chrX: 88759474 - 88759553 |
Synonyms | mmu-mir-1906, Mir1906 |
Description | Mus musculus miR-1906-2 stem-loop |
Comment | None |
RNA Secondary Structure |
Mature miRNA Information | |
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Mature miRNA | mmu-miR-1906 |
Sequence | 48| UGCAGCAGCCUGAGGCAGGGCU |69 |
Evidence | Experimental |
Experiments | Microarray |
Putative Targets |
Gene Information | |||||||||||||||||||||
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Gene Symbol | Tacc1 | ||||||||||||||||||||
Synonyms | 4833447E04Rik, AA960202, B230378H13Rik | ||||||||||||||||||||
Description | transforming, acidic coiled-coil containing protein 1 | ||||||||||||||||||||
Transcript | NM_177089 | ||||||||||||||||||||
Other Transcripts | NM_199323 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Tacc1 | |||||||||||||||||||||
3'UTR of Tacc1 (miRNA target sites are highlighted) |
>Tacc1|NM_177089|3'UTR 1 GAGCCGTCCCGTGAGCTGTGCAGATCCGAGTCTGGCTGCTTCTGTCGTGACCACATTATCTTGCCTTATCCAGAAATAAT 81 GCCCCCCACCCCCCTTTGCAGAGAGAAGTAATTGTTTCAAAGCACATGCCTGCTGCTGCCATCGTCCCCGCTTCGCCGCT 161 GCGCTGCCGCTGCTGCTGCTGCTGCTGCTGCTGCGGCCTGGCTGGTGGAGAGGGAGTCACAAGACAGCTCTGCCCTGCGA 241 CCTGGCTGCCAGCTCCCGTTAGAGCTGCTGCTGGGTCTGGCTTGTGCTTCTACAGCCTGAGTTCATCTAGCTGTCGTCGT 321 CGTCTTTGCTTTTCCCAAGTTTGTGACATTTCTAATTTTGTGGCAACTGTGTGGGTTTATCTTCCCAGCTCCTGTTTGAT 401 GTTTTTATGACCTAGTTAATGTTTTAAGTTTTCAGTATCAGCAGTTTTACTGAAGGGGGCAGTCTGAGTCATCGTCACTA 481 ACACTTGGGGCTCCGAACTCCCTTTTAGTTACAGACGGACTCATGCATGTGTTCTCTACATTCTGCCGCTTTTCCCCTGA 561 GGCATATCCAGATAAGACCCTCTGGGGAGGAGCGTAGTTACAGAAAGAAATGTAGGCTCTTATTTTGAAACAGAGCCCTA 641 GGTCTAAAAGCCAGCTTCAGACTCGGTAGGGGCTGGGAACCTAGCTCTACTGGGGAGTGCTTCCGGTACACAGAGCCCTG 721 GGTTCGGCCCCCAGAACTACAAACACTGGATATTGGAGTGCATGCCTGTGATCTCAACTCTCCAGAGGTGGAGGCAGGAT 801 GACCATAAGTCCAAGGTCACCCTCAACTACATAGCCTCTTGGAGGCCAGCCTGGACTATGTGAGTTCTTGCTTCAAACAA 881 GGAGTGAGTGGAAGCATGGGGTATCCCAGCTAGTGGGAGGCAGGACAGGAAGATCACTTGAGCCCAGAGTTTAAGGACAG 961 CAGTGCACTAGGCAAACAGTCCTTTTCTTAGAAATCAAACACCCAAACCAAACCTTGAGTCAGGGTGGAAAGGCTGAGGA 1041 TACGAGGGCCAGGGGTCATAGCTTTCATGGCTAAGGAAGATTTGCCGTGCTGTCTGCTGCCCCGGTGTTTGGATACCCCA 1121 GGACCTCAGCATGGTCCCTTATGCTCATACCAAAAGCAGCAGCGTGGCCTCCCTCAGAGCTGGCCTTGGATGCATGCGTC 1201 CACCCCGGGGCCGATGAGTCTCTAAAGCAAAGTCAACTTCTGTTATTTATTTGAACCGCACACCAGTGGGATGTGGGAAA 1281 GGTTTAGTTGATCTTGTTAAAAGATGACAGAAGCCCACATTACCAGCCAGAGAGTGAAGAGCCTAGGTTTTCCCTGTTTA 1361 AAGGCACGGCACCCTTTTAGAGACTTAAAAATAACAGCAACAGCAACTGCTTTCATACTGGAGATGGGCTTTTAAACAGA 1441 ACGGGCCGGCCGTAAAGAGTCCTCTGGCTCGTTCTCCAAACCAGCCCCTGGGAATATTCTAAACAAAGACTGCTTGAATG 1521 CTGGGAAGGGGAGCAGGGGGGGAGGTCCACAAGACAAAGCAAAATTCTATGAACTCAGACTGGACTTACCCTGAGGGCGT 1601 TAATCCCCAAATAGACATTTCCCTCTTTGATGCTAGTGACTTCTTTCCATCTATCTAACAGGCAAAGTCAAGTCCACTCA 1681 AGTTTTGATGCCTTTTTTTTTTTTTTTTTTTTTTTTTTGCCTTAATCTTATACCCTGTGTCTTCATGGTAAATGTGCACT 1761 ATTTAGCACACAGGCATATCCCTCCTTGTAAAACCATTGCATGAGCCGACTTCAGCAATGAATTGTGCCTCATGGAGAAC 1841 CTCCATAGATCTTAAAAACAGTGTTCCTTTAGCAGTGTGTTGCCATGTGCTGTAACTTTTAGCCCCAGGGAAGTGAGTAA 1921 TGTCTTTTCAAGCTAGAAATCACATTTTTGTCAATATGCATTTATTATAACCGGTGCTTAGGTTAGGCTATATTCTGAGG 2001 CCTACTGTCTTTTAACATTTTGTACAAAAAAGAAGAAAAAAAACCCCAACAACCCACAAGCAGACAGAAGTTATCTGTGG 2081 TTGGAGAAATGGCTTGACAATCATTTGGGCTCTGAATTTTGTCACAGTGGCTTAATGCAAAGCCCATAGCAGTAGACATA 2161 GTGTCATGTTCAAGTGTCTTCAAGGGTCATAACCAATGTCAGCTTAGCCGTCTTCCGCAAGGGAAGTTAATTTTGCATTT 2241 TAAGAAGCAACGCAGGAATTCTTGCTAGTGTTACGGCGGCATCAGTGTGCGCTCATTTTATATTGACTTATTTTCAGTAT 2321 GGAAAGCTTAAGAGGGTCTCCATCGGAAATCTCTTGTTCCCTGATTTTTTTTTTCTTTTAATAGTACTGAGATTGTACCC 2401 AGGGTCTTGAACATGCTAAGCAAGTGCTTTATCATGGAACTGTATCTTCCCCTGTCATCTTTCTAAGCATGCATATTTTT 2481 AAAGTGTCCTGTGCTGTGCTGTATGTTAGGCATAGGAGAGGAGCAGTGGCTGTCCCTCCCTGCACACTGGCAGACGGGCC 2561 TTGAATAACAGGCCCTGCTAAATGAAGCTGCCGTACAGGGGACATGAACTAGCCCTCTAAGCAGTGTTTGCCAGTGGCCT 2641 CTTAGTCATCACCATTACCAGGAAAATGTTCACAACGTCCAGCTTTCGAAGATCCTCCGCCAAGAATAAGAATGTAGCTT 2721 GCACAACTGGCAAATTAAGAAAATATACTAGGCAGAGTAAAGTCTATTTATTTATTTTGTTTTAATTATATAAGCCCTAA 2801 AAAGCTTTTTAGCACCCTGGAGAGTAAAAAATATAAAGTCGTCTTCAACACACGCTAGGGGCCAAAAGCTTCTTTGTTAG 2881 GTGATTAGAAGGGGGGACCAGGCATGGTGGCACATGCCTATAACCCTAGCACCCTGGAGGCAGAGGCAGGGACTAAAAGT 2961 TCAGGCCTCGACTGGGCTATACCGTGGGGCCCTTGTTTTCAAAGACTTGAAAGCTGTGTCTGGTGGTAGCGTTTGCCGAC 3041 CATGTGCCAGGCACTGTTGCCAATCCCCAGCACTGTCAAACAAGATATAAAACACAGGAGAAAGGCTCACAGTGGCTTGT 3121 GGGATATTAGACGATCTCATTGAGAACCGTTTCTTCAGTTCTGCTTTGCCAAAGTACAGGCAATTCTTAACCTGTCCGTC 3201 AGCATTACAGACTGACCCAGCTTACGGTGGGTCGGAAAGCAGTGCTCGCTCTGTTTGGAGACCGGGAATTCCCCTACACT 3281 TCATATTCTCTGAAGCTCTAATGTGGATTCAGGCATCTTGAGAAACAGACCAAATTAGGAAGTCCTGAAGCACAGGGCTG 3361 GGAGGCCCAGAGTGGATGGGATGAAATCCCTGATTTCAGGGTTATAGCTTAACGCTAAACTTCCTTTCCAGCCAGTGGCC 3441 CTGTTCCCCCTGCAGACATGCCTGTGTACAAAAGCTTGCTCAATCGCTCGCTCACTGGGCAGCCCTGGGCTGAGCATGGC 3521 AGGCCAAAGACTTCCCTTTCAGCAAAGATGGCGCAGGCTAGAAAGGGCACTCTGCGGCTCACAGTCTCTTTAGGATCTGT 3601 GGCCTAAGTTCAGGGCATGCTGAATTGAAAGTGGCGTGGCCCTTCTGAAGCCCCCTTCCCAATTCCCCGGGGCTCCATTT 3681 CAGATGATCTCTCTGCTCAGCTGTTCCTGCTGTGTCTCTGTAGGACTGTGGCCCTCTAAGCTCCCTCTCAGGTTTTGTTT 3761 GTTTGTTTTTTTTTTAAAGTCTTCTTGGGAAGAGAGAATATAGATGTTGAAAGAGTAAATGATTTAGGCAAGGAATTTAA 3841 AGATGTATCTATTATAGAGAATTTTAAAGGTAACAGATTACTATTTACTTACTAATGAACAGATAGGGTCTGGGAGTCCC 3921 AGAACACCAAGCCAAAGGTTGAAGAAATCACCTTTCAAAAAGTTTTAATAATGAATTTATTTCCTGATGTCCAGGAAAGG 4001 TTTGACTGGCCTCCAGGTTACGATTTTTTTTTAAGCAATTTAACAGGGCTGGGGATGTAGCTCAGTTGGTGACACACTTG 4081 CTCAGCATGCACACGTCCCTGGATGTCATCCTCCACCCCCCCACCCCCCAGTTCTGGGGGAAAAAAAAAGAAAAATTTAC 4161 TACTAAGTCCTTCCTGTGTGGAGAGAAAAGGGAACTGCATATAGAGCCAGTGCCTCTCCATAGGCCTTCTGCTAGAACAG 4241 AGTAAGGCAAGCACTTGGCACTGCATAGACTGCACTTCTCGTAGTTTCCATCTGAATGCAGCCCCCCTGTCCGTGCCTCT 4321 CCGCATGCAGTTAGATTTGGACAAATAAGATTCCTGAGGAATGACTCTCTTAATTGCGCCGCGGTTCCAGCTTTATGTAA 4401 ACACACACACACCCCAGTTATAGTTCTAGTTTGGAGATGCTGCTGCCATGTTGGCCTCAGGTAGGCTGTGTGATGTTTTC 4481 ACCACTCAGAGGCCGCCAAGTGTGGGGCTGAGGTCTGGCCTGAACCTTCCCTGTATGGTGTCTTCAATACCAACTGTCAG 4561 GATGCCTCTCTTAGGCCCCTGCCTTCTCGTAGTTTCTGAGAAGGCCCAGCAAAAATGCTTCCCACCCTCAGTTAATAGTA 4641 ACTGATTCTACTATTTGTAATCTTTCAAATGGACACATAGAAATCTGAACTCTGAAACCACATCCAGACCAGATACAGGG 4721 CAGTGTGACGGTCACACCCGCAGCCTTGGCTTTGACCCTTGCAGTCCTTGGGACAGGATTCAGGCACCCTGTTAAGTCTC 4801 CAAGGCATCGGTCAGCTTTCCAGTCCTGCTGTTAGCGAGTCTGTGTTTGCCAATACATACCCACTGTACTAACGTGCCAA 4881 GTGTTTATTGCACATTTGCTCCCACTGTGTCCCAACAACCCCAGCCCCTCCCCTGCCTCCTCGTGCCCTGGAGTGCGTGA 4961 GGAGACCCTCACTGGGGTGGAGGTGGAGGGAGGCACACACTGCCTTGACTCCTGCTGTCAGGACGGTGTTGTGTGGAGCA 5041 ATCATCCTCATAGATTTTATACACATAGTAATTTCCCTTTTTATATGTCAGGTAAATATTTGTAAAAGTTATACTCACAC 5121 AAAAGAAATTATTATGATAATTACTAATATATTTTTTCCATGTTTCATTGCCTGAATAAAAACCGTTTACCACTGTTGCA 5201 AAAAAAAAAAAAAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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Experimental Support 1 for Functional miRNA-Target Interaction | |||||||
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miRNA:Target | ---- | ||||||
Validation Method |
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Conditions | mESCs | ||||||
Location of target site | 3'UTR | ||||||
Tools used in this research | TargetScan , miRTarCLIP , Piranha | ||||||
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2
HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2
... - Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology. |
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miRNA-target interactions (Provided by authors) |
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Article |
- Leung AK; Young AG; Bhutkar A; Zheng GX; et al. - Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
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CLIP-seq Support 1 for dataset GSM4656408 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | mouse liver / P7 |
Location of target site | NM_199323 | 3UTR | GUUAGAGCUGCUGCUGG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE153876 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM4751756 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | adipose tissue / RNA sequencing of iBAT 1 |
Location of target site | NM_177089 | 3UTR | GCUGCUGCUGCUGCUGCUGCUGCUGCG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE142677 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset GSM4751757 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | adipose tissue / RNA sequencing of iBAT 2 |
Location of target site | NM_177089 | 3UTR | CUGCUGCUGCUGCUGCUGCG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE142677 |
CLIP-seq Viewer | Link |
CLIP-seq Support 4 for dataset GSM4751758 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | adipose tissue / RNA sequencing of iBAT 3 |
Location of target site | NM_177089 | 3UTR | GCUGCCGCUGCUGCUGCUGCUGCUGCUGCUG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE142677 |
CLIP-seq Viewer | Link |
CLIP-seq Support 5 for dataset GSM4751760 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | adipose tissue / RNA sequencing of eWAT 1 |
Location of target site | NM_177089 | 3UTR | GCUGCCGCUGCUGCUGCUGCUGCUGCUGCUGCG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE142677 |
CLIP-seq Viewer | Link |
CLIP-seq Support 6 for dataset GSM4751761 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | adipose tissue / RNA sequencing of eWAT 2 |
Location of target site | NM_177089 | 3UTR | CUGCUGCUGCUGCUGCUGCG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE142677 |
CLIP-seq Viewer | Link |
CLIP-seq Support 7 for dataset GSM4751763 | |
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Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | adipose tissue / RNA sequencing of eWAT 4 |
Location of target site | NM_177089 | 3UTR | CUGCUGCUGCUGCUGCUGCGGCC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE142677 |
CLIP-seq Viewer | Link |
CLIP-seq Support 8 for dataset GSM622572 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | mESCs / WT2 |
Location of target site | NM_199323 | 3UTR | UGCCGCUGCUGCUGCU |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21258322 / GSE25310 |
CLIP-seq Viewer | Link |
CLIP-seq Support 9 for dataset GSM622574 | |
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Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | mESCs / KO2 |
Location of target site | NM_199323 | 3UTR | CGCUGCUGCUGCUGCUGC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 21258322 / GSE25310 |
CLIP-seq Viewer | Link |
27 mmu-miR-1906 Target Genes:
Functional analysis:
ID | Target | Description | Validation methods | |||||||||
Strong evidence | Less strong evidence | |||||||||||
MIRT578439 | Irgq | immunity-related GTPase family, Q | 2 | 10 | ||||||||
MIRT578971 | Dhdh | dihydrodiol dehydrogenase (dimeric) | 2 | 4 | ||||||||
MIRT580446 | Tln2 | talin 2 | 2 | 2 | ||||||||
MIRT582494 | Mbtps2 | membrane-bound transcription factor peptidase, site 2 | 2 | 2 | ||||||||
MIRT587588 | Cox15 | cytochrome c oxidase assembly protein 15 | 2 | 2 | ||||||||
MIRT589524 | Mtf1 | metal response element binding transcription factor 1 | 2 | 2 | ||||||||
MIRT591281 | Klc1 | kinesin light chain 1 | 2 | 4 | ||||||||
MIRT591935 | Ddx19b | DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b | 2 | 2 | ||||||||
MIRT592084 | Sorcs2 | sortilin-related VPS10 domain containing receptor 2 | 2 | 2 | ||||||||
MIRT592206 | Mapkap1 | mitogen-activated protein kinase associated protein 1 | 2 | 4 | ||||||||
MIRT592349 | Armcx6 | armadillo repeat containing, X-linked 6 | 2 | 4 | ||||||||
MIRT592351 | Angel1 | angel homolog 1 | 2 | 2 | ||||||||
MIRT592365 | 4930444A02Rik | protein-O-mannose kinase | 2 | 2 | ||||||||
MIRT592427 | Spsb4 | splA/ryanodine receptor domain and SOCS box containing 4 | 2 | 2 | ||||||||
MIRT592671 | Itgav | integrin alpha V | 2 | 2 | ||||||||
MIRT592712 | Fbxo21 | F-box protein 21 | 2 | 4 | ||||||||
MIRT593155 | Itsn1 | intersectin 1 (SH3 domain protein 1A) | 2 | 2 | ||||||||
MIRT593662 | Spen | SPEN homolog, transcriptional regulator (Drosophila) | 2 | 2 | ||||||||
MIRT593976 | Cdc14b | CDC14 cell division cycle 14B | 2 | 2 | ||||||||
MIRT597931 | Pacsin2 | protein kinase C and casein kinase substrate in neurons 2 | 2 | 2 | ||||||||
MIRT597949 | Opa1 | OPA1, mitochondrial dynamin like GTPase | 2 | 2 | ||||||||
MIRT598550 | Isoc1 | isochorismatase domain containing 1 | 2 | 2 | ||||||||
MIRT599017 | Fgd4 | FYVE, RhoGEF and PH domain containing 4 | 2 | 2 | ||||||||
MIRT600307 | Tacc1 | transforming, acidic coiled-coil containing protein 1 | 2 | 2 | ||||||||
MIRT603647 | Otop1 | otopetrin 1 | 2 | 2 | ||||||||
MIRT605000 | Fam168b | family with sequence similarity 168, member B | 2 | 2 | ||||||||
MIRT606275 | Oxsm | 3-oxoacyl-ACP synthase, mitochondrial | 2 | 2 |
miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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