pre-miRNA Information
pre-miRNA mmu-mir-203   
Genomic Coordinates chr12: 112130880 - 112130955
Synonyms Mirn203, mmu-mir-203, Mir203
Description Mus musculus miR-203 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations.
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-203-3p
Sequence 48| GUGAAAUGUUUAGGACCACUAG |69
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Rs1   
Synonyms Rs1h, Xlrs1, tmgc1
Description retinoschisis (X-linked, juvenile) 1 (human)
Transcript NM_011302   
Expression
Putative miRNA Targets on Rs1
3'UTR of Rs1
(miRNA target sites are highlighted)
>Rs1|NM_011302|3'UTR
   1 TGTCTATTTCAGCTCAGTTCTGTCACTTGCAGGGAGAGCTTTGGGGGGAGGGCACCCTGAAATACCACTGGGATGAATGG
  81 GGCTATATCTTATAAGCACTTTACAATGTAGATCTGGGTAGAGAATATTTCACTTTTTAAAAGATAGTTTCAAATTTCAA
 161 AGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGCTTTTAAGCTTTTCTTCAACTCAGGGCCAAAGA
 241 AAATAAGAACAAAGAAAGTATCTCTCAAACCAATTTTCTTACACAAACCCAAATAGCAGGGGTAATTTGCTGCTCTGGCT
 321 CCCCATCTTTTTCTCTTTGTCTCAGTGACACCGTCAAAGGTGCAGGCCCAGGGGAACACAAAGCAGCTCTGATAATTTGA
 401 AAATTCTTTTTGGCTCTTAGCACATTCAGAAGAGGAGCGTACTCTTTGGCAAAGCCTCACATGAACACTTGCATGAAATC
 481 CTCACTATAGAGGCCTGGTGACAAGTACATAGGTGTGAAGAAAGCAGAAGGAAGCCTGGCGTATGGCCCACGAGTCTGGA
 561 GCCAGTAACCAACCAACCAACTAACCAACTAACCAACCAGTCAAGGCTTCCCCCTCCCTGGAATTGCTTTTGCCTGGAAG
 641 GGGTGGAGCTCCTTGTGCAGAAACTCCATTCTACAGCCCTTTTGGCAATGTGTCCTTCTGTGATTGCCTGGAGATGAATG
 721 CAAGGGGCAGGCTATATGTGTGTGTGGATAGTGAGGTTTCCCTTAAACTGAGGAAGCCCAAATAACTAGAATACATCACA
 801 CCTTCTTTATCCATGGCTACAGTTTCCCAGAAATTGAACAGGCTTAGAAAACAGCTGAGATAAACCATATCTCTATCTAC
 881 TGGGAAGAGATCATGCTTTGGGGGAAGGGGTTAGGACTCCTATCATAGAGGACTGTACACAGATGATCTGTCAGTAAAGG
 961 GCTTGCTGTACAGCATAGGAACTTGAGATTATATTTCTAGAATGCATGTAAAAAGCTAGGTATGGACAGGTATGGTGGAA
1041 CATGCCATTAGTCTTAGTATTTGGAAGACAGAGGCAAGCAGATCTGAGTTCAGGGCCAGCCTGGTCTACATAGCAGTTCC
1121 AAGCTAGCCAAGGCTACACAGTAAGGCCCTGTTTCAAGAAAAAAAGACCAGGCATGGAGGGGCACACCTGCAAATCCAGG
1201 ACTAGGAGGCAGGCAGAGACAGGATGATGCCTAGAGCTTGTCTGCCAATCAGTACACTCCAGGTTCATGGTCAGACCCTG
1281 TCTCAAAACTAAAGTGAAGAGCTATTGAGGAAGATACCAGATGTCAACCTCTGTCCAACATGCACACATACACATACACT
1361 GGTAGGTTTCAAACCTGGGGCAAATGGCAGAGGTGCCTAGTTAAGGTTCTCCCAGCATCTCTCAGCCCCTACCAGGTCCT
1441 CCTAGTTGGCATACCCCGCCCCCTACCCCGAAACTCTTCAGCCCAGGGGGTTGGGCTTCTTCCCTATATAATCCAACTAT
1521 TTTGGTCACCCTTTCTCTTTGTACCTTTGGGCCTCCTGGTTGCTGCATCTGGTTCCTCTGTTCTCCCTTATCTCTTTTCC
1601 TCCCTCCTCTCCTCACAAGGCCCAGCTTAGAGTGTTCATGTCTATTCTGGACTCTCTCCCGGATGCCCCTGCCTCTGGCT
1681 ATGCTCTCCCACATATCTACCATAAACTCTCTCCTCTACCATACCTAGGACAAGTCATGTTCCTTTCCTTTTCCTTTTTG
1761 TTTCCCATTCAAACATGTAAATAGGAAAAACAAAATACAACAACAACAAACAAAAATAAAACCACAAAAGCCTTCTCTCC
1841 GAGCGGCTTCCTCCCGTCCTGCCCACCCAAACATGCTTACTCAGACATGACATTTGAAACTTCTTTCGGTAGTCATTCTC
1921 TGGCAGGAATAGCTCATTTAATCAGGAGCTTTGGAGCCTGAGAGAATGACTACTGGTCTCTAGCTCATTTCCATCTGTAG
2001 CTCAAGCTGGGTTTGAGGCTCTTCTCTGGGAGAGGCTGTTAACTGGAGTGAGGGTCAAAAAGGCATGCGGGGAGAAGCTG
2081 AGAATGAGAACTGCCCAGAGACAAGGTCTTGGCCTCACACACACACAATACAGCCTTGGTTAGTACTTTTCTCTGAGCTA
2161 TTTTCTATTTCCAGGTATTAAAGGAGATTACATGGATTCACACCAAAATAACTTTGGATGGCCAGACATGGTATCTTCCA
2241 TCTTTAATCCTAGCACTGGGAAAACACAGACAGGTGGATCTCTGTGAGTTCAAGGCCAGGTTTGCATAGCAAGCTCCAGA
2321 CCAGCCAAGGCTATGTAGTGAGACTGTCTCAAAAATCAATCCATCAATCAAATGATCTTGGATGTCACATTCTTCTCAGT
2401 GAATAGAACAACATTTTAATAGCATCAAATTTCCTACCTAGTATTTTGGAGTTCCACAGGTGTGTGTGTATGTGTGGCAA
2481 TGTACAACAATATTCCTGGTATGACTACATATGTACAAATTCATTTTGGATAATATTCCTGACAGCAGACCTTTATTTGG
2561 AAAATTGAGTTACCTTTCTCACCACACTGAGTAATTGTTAGAGAAAAATGGCAATAACTTAAGAATACTGATCAGGGAGA
2641 AAAAAACCCAAATGGTACAAGGCATGGGTAAATAGTCATCAAATTAAAAAATCATGCCATAAATTTGTTTTCTAATAGTT
2721 TCATTAACTTCACATAAACAGTCCTAATTCATTTAAGGTGTGTAATTCAATATAGTATATTCAAACTTGAACAATCATCA
2801 CTACAAGTAACTTTAGACTATTCTTATCACCCCCAAGAGAATCCTCTAACAATGGGCCATCAGCCACCACAGCTCTAGAT
2881 TACTCGTCATCCATTTTCTATTTGTGTGTGGATTTTTTTTCCATTCTGGGCATTTCTCCACACAAATGGAATCTTAGAAT
2961 ATATAGTAGTTTATGACTTGTTTCTTGTACTTATCATAGTGTTTTTAAAGTCCATGTGTTGGAGAAGTACATCCTCCTTT
3041 TTTGTTATTAACATTCTGTGTAGGGTATTGTATTATTATTTATCCATGCATTTGGGTGGGGCTAGTATGAATAATGCTGC
3121 TTCATGAAAATTTATATGTAAGTTATTGCGTGGACATGTTTTATCTTGGGCATACTCACACTGAGAATAGAGAATTGTTG
3201 GGTCTCTTGGGAACTATTTCTCCAGGTGGCTGTACCATCTTCTGTTCCCACCAGCAATGTCTAATGCTTCCAGCCTCTCA
3281 TCTTTAGCAATGCTTCTTGTATTTCATTAGATCTGTCCTAGTATGTATGAAGTAGCATTCGATGATGAATTTGATTTGCA
3361 TTTCTCTAATAACCAATGTTTTTTGAGCATCTTTTCAAGTCACCAGTGACATATTTTTGGAGAAAGTTCTATTCAGACCC
3441 TTGCCCATATAAAGATTTCATTTCACGGATTCATTAAGAGTTCTTTGGGTGTTCTACATACAATTCTCTTATGCAAACAT
3521 TTCTTCCATCCTGTTTTTCTTCTTGTCATTGTCTTGGTTTAGTTTTGCACCAGGATTTCACTATGTAGCTCAGGCTGGCC
3601 TGGAACTTGCAAAGATCCTTCTGTCTCTGCCTCCCAGGTTCTGAGATTCTGGGTATTTGCCACCACACCTGGCCTTTCTC
3681 TGAAGCACAACAAATACCAAATTTTAATGAAGTCCAACCTTCCGGTTTCCTTTTTTGGTGTATTATCTGAGAAACTACTG
3761 TCAAACCATGGTCAAGAAGATTCACTCTTGTGTTTTCTTCTAAATGTTTTGAAAAAAAATTTTTAAAAAGTTTTAAAGTT
3841 TTAACTCTTACTTTGTGAACAGTTATAAAGTAAGGGTGTAATTTCATTCTTTGGATATCAATATGCAGCTGTCTTGGTAG
3921 CTTGTGTTGTCTTCACCACAGTCAATGGTCTTTATTCTTCCAAAAACAACTGACCAAGAATATTAGAGTATATGCTAGGC
4001 CTCATAATTCTATTACCACATAGTCTTCATTACTGTAGCATTGTAGTGAGTTGTGGAATTAGGACATATAAATCTTCCAA
4081 TTCTGGTTATGTTTCAAGACTGAACTAACTATTCTGGGTTCCTTAAACTTCTAAGTAAGTATATCTGTAAATAAGCCAGC
4161 CAGGGGTCTCACAATGGAAAATGTTTTCAATTTCAAAATTAAATAAACAATGAGATATTAAGATCTGCAAGATATTTATC
4241 TGTAGTTAATTTTCTTTCAATAGTTAATGAAAATGTTAGCATTTTCTTCCTTTTGTGTTAGGATATATATATAAAAGAAA
4321 CCCTATTATTAAAGTAAACCTGAAAAAGAATCTTCCTATTAAACAAAAAATATGTTTGCAATATTATTTCTTTTAGGGGG
4401 CAACTAATTCAATAAAGATTATATAATGGAGGAAAGTCATTCCTATCTTAACACATTTTGTTTATTTTTACCACTAGACT
4481 TTGGGTTATTAAGGATTTTATTTTTTATGGTTTTGAGGACATAACCCAGGTTTTTCATACATGGTAGGCAAATACTCTTC
4561 AATTGAGTTCTCTCTGCAGCCTGCAGATCAAAACAAAACAAAACATTATGGTAAAGCATAACAATTTACATACTTTTAGT
4641 TAACTGTGAAGCATTAAATAATGATATTAAGAAAAAAATGGAAGTAATCCTGGTAGTTGATGTGGGAGGTTATATAGTGT
4721 GGTGGTTTGGATATGCTTGGCCTATTAGGAGGTGTGGCTTTGTTGGAGTAGGTGTGGCCTTGTTGGAAGAAGTACATCAT
4801 TGTGGGAGTGGGCTTTGACACCCTCCTCCTCGCTGCCCGAAAGACAGTATTCTGGCTACCTTTGGATCAAGATATAGAAC
4881 TCTTGGCTCCTTCTCCAGCACCATGTCTGCCTGCATGCTGTTGGCTAGGATGATAATTGACTAAACATCTGAAACTGTAA
4961 GCCAGCCCCAATTAAATGTTGTCTTTCTAAGAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' gaucACCAGG---AUUU-----GUAAAGUg 5'
              | | ||   ||||     ||||||| 
Target 5' acccTTGCCCATATAAAGATTTCATTTCAc 3'
3437 - 3466 146.00 -8.60
2
miRNA  3' gaUCACCAG-GAUU-UGUAAAGug 5'
            | ||||| |||:  ||||||  
Target 5' ctACTGGTCTCTAGCTCATTTCca 3'
1970 - 1993 144.00 -17.00
3
miRNA  3' gaucaCCAGGAUUUGUAAAGUg 5'
               |||   :||:|||||| 
Target 5' atctgGGTAGAGAATATTTCAc 3'
112 - 133 141.00 -13.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions Brain (Mouse neocortex)
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in Chi_BrainC_130_50. RNA binding protein: AGO. Condition:Brain C 2A8 P13 130 KD ...

- Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature.

Article - Chi SW; Zang JB; Mele A; Darnell RB
- Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
Experimental Support 2 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1 HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
34 mmu-miR-203-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004247 Trp63 transformation related protein 63 4 3
MIRT004268 Zfp281 zinc finger protein 281 2 1
MIRT053248 Snora62 small nucleolar RNA, H/ACA box 62 2 1
MIRT053309 Cav1 caveolin 1, caveolae protein 2 1
MIRT437354 ZNF148 zinc finger protein 148 4 1
MIRT437361 Rbm44 RNA binding motif protein 44 3 1
MIRT577405 Ung uracil DNA glycosylase 1 3
MIRT579086 Commd10 COMM domain containing 10 1 1
MIRT579797 Zfp300 zinc finger protein 300 1 1
MIRT580486 Thsd7b thrombospondin, type I, domain containing 7B 1 1
MIRT580726 Srsf12 serine/arginine-rich splicing factor 12 1 1
MIRT580745 Srek1 splicing regulatory glutamine/lysine-rich protein 1 1 1
MIRT582530 Mapk8 mitogen-activated protein kinase 8 1 1
MIRT582806 Katnal1 katanin p60 subunit A-like 1 1 1
MIRT582851 Itga4 integrin alpha 4 1 1
MIRT583438 Frmd3 FERM domain containing 3 1 4
MIRT583523 Fgf16 fibroblast growth factor 16 1 1
MIRT584165 Cpox coproporphyrinogen oxidase 1 1
MIRT584578 Bnc2 basonuclin 2 1 1
MIRT584898 Alkbh5 alkB homolog 5, RNA demethylase 1 1
MIRT585047 Ptchd4 patched domain containing 4 1 2
MIRT585735 Stxbp4 syntaxin binding protein 4 1 1
MIRT586903 Hist2h2be histone cluster 2, H2be 1 1
MIRT587655 Ces2g carboxylesterase 2G 1 1
MIRT590502 Uri1 URI1, prefoldin-like chaperone 1 1
MIRT592154 Pcdhb19 protocadherin beta 19 1 1
MIRT593932 Cyld CYLD lysine 63 deubiquitinase 1 1
MIRT595081 Zmiz1 zinc finger, MIZ-type containing 1 1 1
MIRT595094 Tlr4 toll-like receptor 4 1 1
MIRT595712 Ccdc110 coiled-coil domain containing 110 1 1
MIRT595800 Cp ceruloplasmin 1 1
MIRT600445 Rs1 retinoschisis (X-linked, juvenile) 1 (human) 1 2
MIRT604774 Prkcb protein kinase C, beta 1 1
MIRT606394 Gtpbp3 GTP binding protein 3 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-203 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-203 7,12-Dimethylbenz(α)anthracene (DMBA) NULL 6001 Quantitative real-time PCR mouse skin 24792773 2014 down-regulated
miR-203 Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated
miR-203 17beta-estradiol (E2) approved 5757 Microarray MCF-7 breast cancer cells 19528081 2009 up-regulated
miR-203 Diethylstilbestrol approved 448537 Microarray mammosphere-derived epithelial cells (MDEC) 19549897 2009 up-regulated
miR-203 Cadmium NULL 23973 Quantitative real-time PCR human keratinocytes 19759552 2009 up-regulated
miR-203 5-azacytidine (5-AzaC) approved 9444 Quantitative real-time PCR synovial fibroblasts 21279994 2011 up-regulated
miR-203 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 down-regulated
miR-203 Curcumin NULL 969516 Quantitative real-time PCR Bladder cancer 21836020 2011 up-regulated
miR-203 Arsenic trioxide approved 14888 Quantitative real-time PCR acute promyelocytic leukemia 22072212 2012 up-regulated
miR-203 Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 up-regulated
miR-203 Estrogen NULL NULL Quantitative real-time PCR breast cancer cells. 22260523 2012 down-regulated
miR-203 Interleukin 13 (IL-13) NULL NULL Quantitative real-time PCR bronchial epithelial cells 22453679 2012 up-regulated
miR-203 Goserelin approved 47725 Microarray prostate 22674191 2012 down-regulated
miR-203 Glucocorticoid NULL NULL Quantitative real-time PCR Eosinophilic esophagitis 22815788 2012 down-regulated
miR-203 Glucocorticoid NULL NULL TaqMan low-density array Eosinophilic esophagitis 22815788 2012 down-regulated
miR-203 Tamoxifen approved 2733526 Microarray rat liver 17343880 2007 down-regulated
miR-203 Comfrey NULL 6440495 Microarray rat liver 21370286 2011 down-regulated
miR-203 Comfrey NULL 6440495 Quantitative real-time PCR rat liver 21370286 2011 down-regulated
miR-203 Furan NULL 8029 Quantitative real-time PCR liver 22079235 2011 down-regulated
miR-203 Phorbol ester TPA NULL NULL Quantitative real-time PCR human keratinocytes 19759552 2009 up-regulated
miR-203 Vitamin D3 approved 5280795 Quantitative real-time PCR human keratinocytes 19759552 2009 up-regulated

Error report submission