miRTarBase - #MIRT600919


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pre-miRNA Information
pre-miRNA mmu-mir-326   
Genomic Coordinates chr7: 99552269 - 99552363
Synonyms Mirn326, mmu-mir-326, Mir326
Description Mus musculus miR-326 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-326-3p
Sequence 60| CCUCUGGGCCCUUCCUCCAGU |80
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Exoc8   
Synonyms AI414418, EXO84, Exo84p, R74783, SEC84
Description exocyst complex component 8
Transcript NM_198103   
Expression
Putative miRNA Targets on Exoc8
3'UTR of Exoc8
(miRNA target sites are highlighted)		 
>Exoc8|NM_198103|3'UTR
   1 GGCTCTGCTCTGTGTCTTTGTGTGTGTACGTGTACACGTGCTACAGATGCGTCATTTGTATTTACATTACACAATGTCAG
  81 CACATTCCTCAGAGACACAGACAGCATATAAAAGTGCCCTCTGAAAAATGCAGTCAAAAGGAGAAAGATAATGTGTTAAG
 161 CATCACATACAACCCCCCAATACTGGGACTGTCAAGAGCACATACCATGTCTTGCTCTGTTTTTTAATAATTCTTTAAGT
 241 AGAGTGTATGGTCACACCAGAACATTTCAGGCTACTCTTTAACTTGAAAGACACTTCTCACGTTAGCACATCTAGGAGCA
 321 GTGCACTTCCATGCTGCCTAGAAAAATATTAGGTCCACAGGGTTTAAGTATGTTGTAAAACATTCCAAGTCAACAAAATG
 401 GCTTCGTAATGGTCAGTGCTGTGTGACACACCTGTGTCCTGTACTGAGACAGATCAACATGAAAAGAAATGAGTTGGGAA
 481 AACTGGTTTGGGATAAATTTCAATATGTGGGCCTCAGGTCTGTCAGTATTTGTAGCATGTGGAAGGAAGCCTTTATAAAA
 561 CTTTGCAGGCATCCAAAAGTCACTGAAAAGTAGCATGTTCCATGGCTAATTGGATGCATCTTTTTGGGGGAGGTGGGAGA
 641 ATCACAAGGACTACGTTGAATTTTTAACATATTCCAGAACTGAATGGATTTGCTGCTGCAGCGTGTTTTGCCATGTTCAG
 721 GAAGTAAGTCTTGCTGAAAAGCCACACCAAGGATCCTAGCAGTAGGCGGGTAAGTGACTCCAGCCCCTGCAGACACTTCA
 801 CCACCAGCCTGGGTTTGGAGGCACTGAGACTTCTGGCAAATGCAGAACTCCCTGTCTGAGAAGAGTTCAGGCTGTGTGTA
 881 GGTTAGGAGTTTTAAAAAAAAGAATGCAATGTCACTCCTTAAGAATGCCAGAGATTTGGTTTTGGTCATTTTGTTTTCTT
 961 GAGATAAGTTTCAGCCCAGACTGGTTTTGGGCTGGTGCTGTAACCAAGGATGACCTTGAATTTCTAATCCTGACTCCACA
1041 TCCCAAGGGCTGGGATTACAGGAATGTGCCATAATGCTATTTATGCTGTGTTGGAGAAAAAAAACCCTGGCTTTCTCATA
1121 CTAGGCAAGCACCCTACCCACTGCTACACCTGCTGCCCTGATGGTTTTTGAAACTTAGTAACTGAGTAGAGATGCACATA
1201 CCTCAAGCACAGGGAAGGGACCTTCTGTGACTCTTCAAATGCTGTGCTGCATTTTAAAAAACAAGAGCCTGGCATGCTGG
1281 TGCACACTTTTAATCAGAGCACTGGGAGGCAGGAGCAAGCGGATCTCTGAGTTCAAGGCCAGCCTGATCAACAAGGTGAA
1361 TTTCAGGACAGCCAGGGATCTGTAGAGACCCTGCCTTAAAAACAACTTTTAAGAGAATTCCAGATTGTAATACTTACTTC
1441 AGGTTGTTGAGTAGGGATAGGTGGGTAGGTGGAGCCCCTAAGCTTTTATTTTTTTTTCTTTTTCTTTTTTTGGTTTTTTT
1521 TTTGTTTTTTTGTTTTGTTTTTTGTTTTTTCAAGGGTTTCTCTGTATAGCCTTGGCTGTACTGAAACTCACTCTGTAGAC
1601 CAGGCTATCCTTGAACTCAGAAATCCTCCTGCCTCTGCCTCTGCCTCCCAAGTGCTGGGATCAAAGGCATGCGCCACCAT
1681 GCCCGGCTTGCCCCTGATTTCTAAAGTGAGCTCTGCTCTGATCACTTAATGTCCTGTTAGAATGTGGGTGCTGAAACCCA
1761 TTTTCCCACTAGCTTGTAGCTGTGGGTTTTGTCAACGATGCCATGTTTTTATGTCCATTGGGTCTAAAGGCTATTTCTTG
1841 TCTCTTGAATAAAAGAGGTAGTAGTAAGCCATAAAATGATGTAAAACCCTAAATGGCTTCTGCAGTGATTCAGGGTTGTT
1921 AAGACTCCTCAGCTTCATTAACTCATCAGGCCAGCAGTTTCTCTGTCTTTTTCTAGCTCTCCATGCATAGAAAGATCGCA
2001 GAGGTCACCACTGCAGCCTTCCACTTTCCCATGTTAGGGTTTGTTGATTTAAATCAACAATGAAGACCAAGCGGGTCTTG
2081 AACTCACAGAGGTCATCCTGCCTCTGCCTCCTGAGTGCTGGGATTAAATTATGCTCCACTATGCCTTGCTAAGTTAGAGT
2161 TTTCTACATAACCATAACTGGATGTCCCAGCCAAACAAATGTACTCATTTCTCTTAAGCTCACTGGACTTGGATTTGATG
2241 AAATTGTGTGGCTGCGATGCCGATTTGGTGCCACTTGGAATTTAATAGATAAACCGTGCATAAGGCCTCGAGGTGTAAGA
2321 AGGCTAACATGTTTCCCCACATAATAAATGGCTGTATCTTTATGGAAATGGAAGTAGAGAACTTTATTCTGCTAAGAACG
2401 GGCTAATGTGTTCCTCTGTGTAGCTGAGGGAATGCAGTCTGGGAAATCTGCAGGAAGCTTGAAGGATTGTCTAGCAGTGG
2481 TAGTTCCACACATGCTGACGTGCTGTGACATTAACCACCTTTTGTTTGCTTTAAAACTAACTGCATAGACCAGGAATCAC
2561 AGACTGGGTTAATTTCAGTTAGAGAATTCAGCCTCTGATGAGAGCCTCCTGTCATGAAATGCTCAAAGTCATTGCTGTGA
2641 CTTTGGTATTACCGTGATTGCCAGCACATTCATCCTGCACCTGGTATTAGTTAGGGCTAAGCAGCTGGGGCTTGCTGCAG
2721 ATGCAAGCATTGCTGAACACTACACTTTGATGTACTGTGAGAAACAACCTTTAAACTGATAAAGCTTTATAGCATTCTCA
2801 CTGGATAGAACTGATTGCTATAACATTCTGTTTATTTACTTGTTTTGAGACGGGTCTCACTCTAGTCCTCTGCCTCCTGA
2881 GTGCTGGGATTAAAGACATGCTCAGCTAGGTGTGGTGACACACAGTTTTTATTCCAGAATTGCAGAGGCAGAAGCAAGAG
2961 GATCTTTCGGTAGCCAGGGTTACCTAGTGAGACAGACCCTGTCTGAGAGAGAGAGCCAGGCCTCTCGGCACACCCTTTAA
3041 TCCCCAGCACTTGGGAAGAAGCAGGCAGATCTGAGTTTGAGGCCAGCCTTGTCTACATAATGAATTCCAGGACAACTAGG
3121 ACTACATAGATGCTGTTACCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAGAGAACTGGAGAGATGGCTT
3201 AGTGGTTAAGAGCACTGGCTGCTCTTCTAGAAGTCCTGAGTTCAATTCCCAGGAACCACATGGTGGCTTAAAAAGAAAAC
3281 AAGCAAACAAAAAATAACATTTGCTGCTCTCGAGGGCAGGCTCAGGATTAGTACGCAAAATCATTAGTCCACTTTCAGCA
3361 AATGACACCTTCTGGCCTTGGGTACTGCACACGTGTTCATACACAATGTAAAAAATCTTTGAAAACTTGTAGTAAAAGTT
3441 ATACGTATGGATAGATTGTGTGCGCAATATAAGCCTTTCCTTGCTTATCTTGTTTATGGGAATACATGTATAAAGTTCAT
3521 GTCTAGCTAGGTGCTGCTATGCCAAACCAGGCCCTTTTTGTTGTTACATACATTAACCCATCTACTGTGTCCTGCAGATG
3601 TCTCCAACGGTAGTGTTTCTCCTGGCCTTTTAGCTCCTCTGGTGGCAGACTTGACTGTGGCAGAAGGAATTGTCTTATGC
3681 ATCCAGGTCCCTCTTGTCACGGTTTTGATACAGGAACCACCATGTCAGATGTCCATGGTCCACTTCTTGGCCACAGGAGC
3761 ATTAAACTTGGTGTGCTGGCTGATTTCACCTTTACTCACCATTTTCCTGGAGTTACCCACGATGCCTTCTTGGTGCATTT
3841 GCTGTGACACAGAGAACCAAATCCCAAAGAACCAAACTATCCTTTCTTAATTTAGATAAACACTGCCAAACTTGACCACC
3921 AGGAAAGTTGTGTAGTCTCCCCAAACGACAGGAAAATTCTATTTCCATTAATCCTCATAATTATTCATTTTGTGATAAAT
4001 GTAAACTTCCAATGTAGTAAGACTTAAAACCACCTTTGTTTGTTTGTGAGATAGGGCCTGTGTAGCCTCGAACCTCCTTG
4081 GAAGCCTATTTAGGTCAGGTTGGTCTTGAGCTGCAGGACCCCATTGCTGCTGCCTCACATGTAGAGAAAAGGTATCACCA
4161 CACCAGGCTTATTCACTCAGTTATGTGACAGTGTGTCTTGGTGCTGGAAAGCAGGTGTTAAAAGCCCTGGAGCTGGAGTC
4241 GCAGGTGTTCCGGGTCACCTAACGTGGGTGGAAGGATGTGAACTCAGGTCCAATGGAAGCGCAAGTGCCCTTACCTTTGT
4321 GCATCTCTCCAGCCCCACATTTATGTACACTTTGAATAATACTCTTTTTGAGAGGGGGATGAGGCTTAGTACAGTGCTTG
4401 CCTAGCATGCACAAGGCCCTGATCTATCCCCAGCACTGTACACACTGCATGATAGTGCATGCCTGTAATCTCAGCACTGA
4481 GGTAGAGGCAGGAGGATCTGAGGCTCGCTCATCCTCAGCTACCTGGTGAGTTCAAGGCCAACACCCGACTGGTTGCAGCC
4561 TGTGTTGTTGGGTGATTAAGAACATTTGCTGCTCTTGAGGGCAGGTTCAGTTCCCAGGACTCAGGTAAGCAGCATGGGCG
4641 TGTTGACACGTGCCTGCAGCTGATAGGGCTGTGACCTGCCCACCAGTCCACAGCCAGGGTCTCTGTTAAATGGGACTGCT
4721 TATTTCAACATGCAGGAATCCCCTCTAGCTTCCCAAAGCAGCAGATGCTGTCAGAGCACCTGTCTGTCACTTGTGGCCTA
4801 GCACAGCGGCTGCCCGAGGCCCTGGGAATAGATGCACAACCTGGGGATTTGGAGACAGAACCACACAGAGTTTCAGTGAC
4881 TTTGCTTGGTTCTCAGACTCAAATGCCTGTCCCCAATGACTGAACGGCACCCCAGATGCATATACTGATGAGAATGAACC
4961 CCTCAGGATGAGATCAGTCATTATTTTGTTTTGGGGGGGTGCAGGATGTACTGAAAAGATCAGAAAGGACCAGTGCCTGT
5041 CCACTTTCTGTCCACTTTCTGTGGTCCTGAGTGCGCATGGACTCAGCGCCCATCCAAACCTAAGGCGAACCTGTGAGAGC
5121 CTTCATTCTGGACTTCAGCTTTCAGGACACAAAAAATAAAAGGCTGTGTTGAGGGCAAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugACCUCCUUCCC---GGGUCUcc 5'
            ||   ||| ||   ||||||  
Target 5' aaTGACTGAACGGCACCCCAGAtg 3'
 4915 - 4938 131.00 -13.90
2 
miRNA  3' ugACCUC-CUUC----CCGGGUCUcc 5'
            | |||  |||     |||||||  
Target 5' tcTTGAGATAAGTTTCAGCCCAGAct 3'
 957 - 982 129.00 -12.20
3 
miRNA  3' ugACCUC---CUUCCCGGGUCUCc 5'
            |||||   |||   | ||||| 
Target 5' ttTGGAGACAGAACCACACAGAGt 3'
 4848 - 4871 127.00 -14.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ugACCUCCUUCCCGGGUCUCc 5'
            ||||| :| |||::|| | 
Target 5' acUGGAGAGAUGGCUUAGUGg 3'
 		4 - 24
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
23 mmu-miR-326-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT004432 Smo smoothened, frizzled class receptor 3 1
MIRT005596 Ets1 E26 avian leukemia oncogene 1, 5' domain 3 1
MIRT581306 Rnf150 ring finger protein 150 1 2
MIRT582546 Map3k7 mitogen-activated protein kinase kinase kinase 7 1 1
MIRT582559 Maff v-maf musculoaponeurotic fibrosarcoma oncogene family, protein F (avian) 1 1
MIRT582898 Ildr2 immunoglobulin-like domain containing receptor 2 1 1
MIRT583744 Ephb3 Eph receptor B3 1 5
MIRT590217 Dync1li2 dynein, cytoplasmic 1 light intermediate chain 2 1 2
MIRT590852 Vsx1 visual system homeobox 1 1 2
MIRT593069 Ccdc25 coiled-coil domain containing 25 1 1
MIRT596778 Usp22 ubiquitin specific peptidase 22 1 1
MIRT596972 Tmem179 transmembrane protein 179 1 1
MIRT597063 Tbx5 T-box 5 1 1
MIRT597081 Tars2 threonyl-tRNA synthetase 2, mitochondrial (putative) 1 1
MIRT598144 Mylip myosin regulatory light chain interacting protein 1 1
MIRT600897 Fbxl16 F-box and leucine-rich repeat protein 16 1 1
MIRT600919 Exoc8 exocyst complex component 8 1 1
MIRT604218 Clgn calmegin 1 1
MIRT604799 Phactr1 phosphatase and actin regulator 1 1 1
MIRT605009 Ept1 selenoprotein I 1 1
MIRT605050 Efr3b EFR3 homolog B 1 1
MIRT755982 Th tyrosine hydroxylase 3 1
MIRT756011 Tlr4 toll-like receptor 4 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-326 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-326 Propranolol approved 4946 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-326 Dexamethasone approved 5743 Microarray adrenals and granulosa cells 24205079 2014 up-regulated
miR-326 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 down-regulated
miR-326 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated

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