miRTarBase - #MIRT601211


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pre-miRNA Information
pre-miRNA mmu-mir-466e   
Genomic Coordinates chr2: 10479088 - 10479171
Synonyms mmu-mir-466e, Mir466e
Description Mus musculus miR-466e stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-466e-5p
Sequence 12| GAUGUGUGUGUACAUGUACAUA |33
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Arhgef9   
Synonyms 9630036L12Rik
Description CDC42 guanine nucleotide exchange factor (GEF) 9
Transcript NM_001033329   
Expression
Putative miRNA Targets on Arhgef9
3'UTR of Arhgef9
(miRNA target sites are highlighted)		 
>Arhgef9|NM_001033329|3'UTR
   1 TACCTATAGGAAGGCAGATAATTTTAAAATAAATAAAATTATATTTATAGATGGACCTTTTTTTCGGAGAAGCACTGTTG
  81 AAAATTTATATATATATATATATATAAAATGTGTGTGTGTGAGAGAGAGTGAGTGTGTGTGTGTGCGTGTGTGTGCACAC
 161 ATACATGCACACACACACACGCACACATAGACCTTTACGTATACACGTGTGTGTGTGGGCATGTGTGCACATATGCATGC
 241 ACATGCATGCACACATACATACATATACACACACAGAGAAGGACCAAAACCATTTTCTGTTGTTTTGGGGAGTTGAAATC
 321 TGTAGTTGGTAAGAAGTACCACTGATTTCCAAACATGTAATCACTTCTTCAAATTTTTAGTTCTTAACCTTGTTTCCTCC
 401 CCCTTACTCTCTGTTATTTTTCCTGTTAAGATAACTTATTCGCTCCTCTAGTTCACTGACCTGTCATTATTACCATAGAG
 481 TATTTGCATTGTTTTATTTCCCTTTCCTTATGATACTGACGCTGATAACTTTTGAATTGAATTTATGTAGTGGGTTTGGG
 561 ATTTCATTTCTCTCCCTGTCACTTTCCACTGAGAAGGGGAGGATTCCCGTCTCCTGTTCCTCATGCACCCATCTGCAAAT
 641 GTTCCTGTAGCCCTGCAGTTATACCAACAGCCTCTTCCCAGCATCCTGTGTCCACAATTGTGCCAGTCTATACATGCTCT
 721 GTTGTGCCCTGTGACTTATCTGCTTGGTATTTCTATTGCTTTCACTGTTTTAGGTATTGTGGAAGGATATAAAACCAAAC
 801 AGAAACAAAGGAGTGAACGAATGTAATGAGAGTCGAGAAGATTTCTGTTAGGGGGATAGTTTTTATTTAGGTGATTTCTT
 881 CCCATATAACAGCAATGCTAAGCCATTGAAAGTTTCCACCTAAGAATTAGTTTGGGTCCATTAGTCTCTTTTCCAAGCCT
 961 CTTTTGTTATGGATCTCCTTTTCAGTCTATCTCTTCCCTAGGCAAAAGGTTTAACTAAACTAGTAGCAAAGAAATTGAGA
1041 CTTTGCATCCTGGGGAAGAGATTTATCTCCACCCCATAACTCATAGATGGTAGGTAGGTGTCAGGCAGGGCTCTCTTTAT
1121 AGTGTTCTGGTAACCCCTGCTTCTGTTTTCAGATTCATAAGGCATCATTGTTAGTAGTTTATGGAGGCGTTCAAGAGAAA
1201 AACCTGCTTGGCCAAGAGCATTAGCCACCTTCAGATTAACCTGAAAAGAGTTCAAGGTTGACAACAGCATCTTATAGAGA
1281 CCATCAGGAAAAGAGAGAGGCTTGATCTAAGATAATATACCAGTAATTTGCATCACTGAAATTATAAGAAACATCCCCAA
1361 TTGTTAGCAGATTGCATGGATAATCTCTGTCTCCTTTTTTTGCCCTTACTGTCTTTCAGGTAGAGGATTGCGAGTATGGG
1441 ATAGTTAGAGCCATTATGGCACTCAAAGCAGGCTTTGTTCCAGACAGACTCCCATCCCAGGACAACTGAGGCAAGTGGTA
1521 GAGAAGCCTAGGGCTACTTTTACATCTGGGGGAAAAGAAAAGATCTGAGGTAGAACACTGAAGTTATTTCTTGGCAGGGT
1601 TTCCATTTGACCAGAATGTTTCCCACAAAAATCAGAATCCTCATGAGTACAAGAGTGCTTATTCTTTGTCATTTAGCCCT
1681 TTCTATGGCTAGAGATTGGAGCAAGAACAATAGAATATAAAGAAGTGTGAAAACCCACCCTATCCAGGAATCTGGAGATT
1761 GTGTCTGTCCTGGACCTCACAAGAAAATACTTCATTATAAAAAAAAAAGATCTAAGCTTACCAAGTAGCAACCATAGCTG
1841 TATGAGGTCTTCAAAAAAGCTGAATTTGTACTTCTTGACCTAGATTAAGCCCTACCCCTGAGAATCTTTCCCCTCTGAAA
1921 AACAAATTACACATTGTTGTCAGACTTTGTAGTTATCAAGGCTAACTAGCAGAATACCAGGGCTCGGGACTGGCTATCAC
2001 TGCTCAGCATATGATAGAGCTACATAAGCCCTTATGGCAAGAAATCTGGCATCGAGCACATAGACTACAATGCTCTCCCT
2081 TGGCAAACTGAAATGTGTTTCACTTCCTATGCAGGCCTCCCTTATTGCACCTTCATTTCCTACTTTCTACCCATTCTCAC
2161 TAGGCTTGGCCTTGGCAACTTTCTCCCAGGGTTAGGAGCACACCAGGAGGGCTTGTTTTGAAAGGTTTCTAGATCTATAG
2241 TATCTTTTGGCCAGACCTTCTCATTCTTTTCCCCTTTCTGAGTCCAACATTCAAGGCTCCCAGATCAGGGGTATTACCTC
2321 TCATCATCCTGTTTCTCCATCCAGTCCACTAACCTTACAAGATGTAGTAATTTGTGAAGGCACTACCAACTCCGCTCTCC
2401 TTGGATTGGAATGCCTGGCTCCACCCTCACACCTACAGCTCCAAATAGATCAGTGGCCAGTAGCCCAAAGGGAAACTCAT
2481 CAATTCCCTGGACTCAGTAGCTGAACCTGCTGCAGCCCCACGGCCATATAATCCCCCCCAAAAATAACTCTATACTTCTC
2561 CTGTTTCTGCATGGCCCCAGACTCCCCTTTCCTCAACTCAATGAGGCCCGAACTTTCTTTCTCCAGTTTTGTGAGTTTGT
2641 TCTATGAGTTCTATTATAGGGAGGTCCTAAATACAGAAATACAATTTTTCTCTCCTTCTCTCATCCACTTTCTATGTGTA
2721 TATCTTTAATTGATCTATTAAGGACAGCAGCAAGAGAGTTCTAACAATTCTAGTATGAAGCCAAATAGTGCTATTTTAGT
2801 GCTTTGGGGGTGGGGCGGGCTGGGTGACTAGATGGGCAACAGTGATTTTGACCCCTGCTGCTCTGCATTTGCCGGCTTAT
2881 TCTTTTGTTTCTATTATCCGACTGTTTGACCCTGTCAAACAAGTGTCAAAATTGTGTATTTGGAAATGTTTAACAACAAC
2961 AACAACAAAAAAGATGTTGTAATGACATAAAGCCTTATGAAAATATTTATGGAGTTCAATAAAAGAAGTAAAAAAGAAAA
3041 AAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' auACAUGUACAUGUGUGUGUAg 5'
            || ||||| |::||||||| 
Target 5' caTGCACATGCATGCACACATa 3'
 236 - 257 176.00 -17.40
2 
miRNA  3' auACAUG--UACAUGUGUGUGUAg 5'
            |||:|  :|||:::||||||| 
Target 5' tgTGTGCGTGTGTGTGCACACATa 3'
 140 - 163 170.00 -22.40
3 
miRNA  3' auacaUGUACAUGUGUGUGUAg 5'
               |||   ||:||||||| 
Target 5' cacacACACACACGCACACATa 3'
 168 - 189 157.00 -14.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622574. RNA binding protein: AGO2. Condition:KO2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' auACAUGUACAUGUGUGUGUAg 5'
            |||:::|||:::|:::::| 
Target 5' agUGUGUGUGUGUGCGUGUGUg 3'
 		16 - 37
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
CLIP-seq Support 1 for dataset GSM622574
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / KO2
Location of target site NM_001033329 | 3UTR | GUGUGAGAGAGAGUGAGUGUGUGUGUGUGCGUGUGUGUGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
145 mmu-miR-466e-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT410978 Ado 2-aminoethanethiol (cysteamine) dioxygenase 2 2
MIRT417624 Lifr leukemia inhibitory factor receptor 2 2
MIRT432330 Igf2 insulin-like growth factor 2 2 4
MIRT577332 Zfp157 zinc finger protein 157 2 2
MIRT577683 Slc39a14 solute carrier family 39 (zinc transporter), member 14 2 6
MIRT577854 Rassf5 Ras association (RalGDS/AF-6) domain family member 5 2 2
MIRT578123 Nqo2 N-ribosyldihydronicotinamide quinone reductase 2 2 2
MIRT578174 Neu1 neuraminidase 1 2 2
MIRT578293 Mavs mitochondrial antiviral signaling protein 2 2
MIRT578540 Htr1f 5-hydroxytryptamine (serotonin) receptor 1F 2 2
MIRT578635 Gtf2h2 general transcription factor II H, polypeptide 2 2 2
MIRT578659 Gramd1c GRAM domain containing 1C 2 2
MIRT578883 Fam107a family with sequence similarity 107, member A 2 6
MIRT578911 Entpd1 ectonucleoside triphosphate diphosphohydrolase 1 2 2
MIRT579075 Cox15 cytochrome c oxidase assembly protein 15 2 4
MIRT579095 Cnih3 cornichon family AMPA receptor auxiliary protein 3 2 4
MIRT579133 Chrna1 cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) 2 2
MIRT579301 BC021785 major facilitator superfamily domain containing 4B5 2 2
MIRT579361 Aplnr apelin receptor 2 2
MIRT580013 Whsc1l1 nuclear receptor binding SET domain protein 3 2 4
MIRT580126 Ubn2 ubinuclein 2 2 2
MIRT580259 Trim12c tripartite motif-containing 12C 2 2
MIRT580358 Tmem26 transmembrane protein 26 2 2
MIRT580397 Tmem170b transmembrane protein 170B 2 2
MIRT580836 Smarca2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 2 2
MIRT581003 Six4 sine oculis-related homeobox 4 2 2
MIRT581112 Sept3 septin 3 2 2
MIRT581333 Rgs8 regulator of G-protein signaling 8 2 2
MIRT581426 Rasal2 RAS protein activator like 2 2 2
MIRT581434 Rasa2 RAS p21 protein activator 2 2 2
MIRT581473 Rabgap1 RAB GTPase activating protein 1 2 2
MIRT581601 Prkcd protein kinase C, delta 2 2
MIRT581808 Plag1 pleiomorphic adenoma gene 1 2 2
MIRT581914 Pgm3 phosphoglucomutase 3 2 2
MIRT582069 Onecut2 one cut domain, family member 2 2 2
MIRT582600 Lrrc40 leucine rich repeat containing 40 2 2
MIRT582752 Klf8 Kruppel-like factor 8 2 2
MIRT583022 Htt huntingtin 2 2
MIRT583260 Gnb4 guanine nucleotide binding protein (G protein), beta 4 2 4
MIRT583313 Gfod1 glucose-fructose oxidoreductase domain containing 1 2 2
MIRT583618 Fam46a family with sequence similarity 46, member A 2 2
MIRT583813 Eif2s1 eukaryotic translation initiation factor 2, subunit 1 alpha 2 2
MIRT583908 Drp2 dystrophin related protein 2 2 2
MIRT584442 Ccdc85a coiled-coil domain containing 85A 2 2
MIRT584656 B4galt6 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 2 2
MIRT584859 Appl1 adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 2 2
MIRT584912 Akap10 A kinase (PRKA) anchor protein 10 2 2
MIRT585294 Zfp26 zinc finger protein 26 2 2
MIRT585429 Wars2 tryptophanyl tRNA synthetase 2 (mitochondrial) 2 2
MIRT585487 Txlnb taxilin beta 2 2
MIRT585694 Tbc1d24 TBC1 domain family, member 24 2 2
MIRT585739 Steap2 six transmembrane epithelial antigen of prostate 2 2 2
MIRT585878 Slc22a8 solute carrier family 22 (organic anion transporter), member 8 2 8
MIRT585907 Slc17a5 solute carrier family 17 (anion/sugar transporter), member 5 2 2
MIRT585960 Sike1 suppressor of IKBKE 1 2 6
MIRT586177 Ptprr protein tyrosine phosphatase, receptor type, R 2 2
MIRT586589 Mrgpre MAS-related GPR, member E 2 2
MIRT586806 Ints8 integrator complex subunit 8 2 2
MIRT586919 Heatr2 dynein, axonemal assembly factor 5 2 4
MIRT586945 Gstt3 glutathione S-transferase, theta 3 2 2
MIRT587123 Gfpt1 glutamine fructose-6-phosphate transaminase 1 2 2
MIRT587151 Gcnt4 glucosaminyl (N-acetyl) transferase 4, core 2 (beta-1,6-N-acetylglucosaminyltransferase) 2 2
MIRT587239 Nxpe3 neurexophilin and PC-esterase domain family, member 3 1 1
MIRT587482 D630045J12Rik RIKEN cDNA D630045J12 gene 2 8
MIRT587489 D630003M21Rik RIKEN cDNA D630003M21 gene 2 2
MIRT587602 Cml2 N-acetyltransferase 8 (GCN5-related) family member 2 2 2
MIRT587664 Cdk7 cyclin-dependent kinase 7 2 2
MIRT587741 Cd28 CD28 antigen 2 2
MIRT587752 Cd200r1 CD200 receptor 1 2 2
MIRT587775 Ccpg1 cell cycle progression 1 2 2
MIRT587839 Casp8 caspase 8 2 4
MIRT587959 Asah2 N-acylsphingosine amidohydrolase 2 2 2
MIRT588288 1700019G17Rik N-acetyltransferase 8 (GCN5-related) family member 4 2 2
MIRT588355 Zfp518b zinc finger protein 518B 2 2
MIRT588392 Zeb2 zinc finger E-box binding homeobox 2 2 2
MIRT588548 Uhrf1bp1l UHRF1 (ICBP90) binding protein 1-like 2 2
MIRT588604 Tsc22d3 TSC22 domain family, member 3 2 2
MIRT588726 Tbx22 T-box 22 2 2
MIRT588954 Scn2a1 sodium channel, voltage-gated, type II, alpha 2 2
MIRT589780 Insig2 insulin induced gene 2 2 4
MIRT589840 Hif1an hypoxia-inducible factor 1, alpha subunit inhibitor 2 2
MIRT589863 Hecw1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 2 2
MIRT589975 Gabpb2 GA repeat binding protein, beta 2 2 2
MIRT590188 Ell2 elongation factor RNA polymerase II 2 2 2
MIRT590360 Clca2 chloride channel accessory 3A2 2 2
MIRT590488 Calcoco1 calcium binding and coiled coil domain 1 2 2
MIRT590659 Apba1 amyloid beta (A4) precursor protein binding, family A, member 1 2 2
MIRT590843 Wdr46 WD repeat domain 46 2 2
MIRT590859 Vps33b vacuolar protein sorting 33B 2 4
MIRT590920 Tacr2 tachykinin receptor 2 2 2
MIRT590936 Supt7l suppressor of Ty 7-like 2 2
MIRT591004 Sh2d2a SH2 domain containing 2A 2 2
MIRT591018 Scd3 stearoyl-coenzyme A desaturase 3 2 2
MIRT591037 Rfx3 regulatory factor X, 3 (influences HLA class II expression) 2 2
MIRT591045 Rassf2 Ras association (RalGDS/AF-6) domain family member 2 2 2
MIRT591136 Nsun3 NOL1/NOP2/Sun domain family member 3 2 2
MIRT591225 Ly96 lymphocyte antigen 96 2 2
MIRT591308 Il18r1 interleukin 18 receptor 1 2 2
MIRT591349 Flrt1 fibronectin leucine rich transmembrane protein 1 2 2
MIRT591363 Egfl6 EGF-like-domain, multiple 6 2 2
MIRT591410 Cer1 cerberus 1, DAN family BMP antagonist 2 2
MIRT591443 Bcl2 B cell leukemia/lymphoma 2 2 2
MIRT591521 Abcc9 ATP-binding cassette, sub-family C (CFTR/MRP), member 9 2 2
MIRT591577 Zfhx3 zinc finger homeobox 3 2 4
MIRT591597 Xrcc3 X-ray repair complementing defective repair in Chinese hamster cells 3 2 2
MIRT591614 Vps37a vacuolar protein sorting 37A 2 2
MIRT591708 Rpusd2 RNA pseudouridylate synthase domain containing 2 2 2
MIRT591720 Rorb RAR-related orphan receptor beta 2 2
MIRT591740 Rab9b RAB9B, member RAS oncogene family 2 4
MIRT591815 Mocs1 molybdenum cofactor synthesis 1 2 2
MIRT591838 Lin7a lin-7 homolog A (C. elegans) 2 2
MIRT591850 Iglon5 IgLON family member 5 2 2
MIRT591897 Fbrs fibrosin 2 2
MIRT591978 Ap1ar adaptor-related protein complex 1 associated regulatory protein 2 2
MIRT592164 Oxtr oxytocin receptor 2 2
MIRT592241 LOC100048884 major urinary protein 18 2 2
MIRT592285 Gatc glutamyl-tRNA(Gln) amidotransferase, subunit C 2 2
MIRT592450 Ski ski sarcoma viral oncogene homolog (avian) 2 2
MIRT592466 Runx1 runt related transcription factor 1 2 2
MIRT592683 Has2 hyaluronan synthase 2 2 2
MIRT592831 Asb7 ankyrin repeat and SOCS box-containing 7 2 2
MIRT592853 Akap2 A kinase (PRKA) anchor protein 2 2 2
MIRT593348 Tmco1 transmembrane and coiled-coil domains 1 2 2
MIRT593391 Adra1b adrenergic receptor, alpha 1b 2 2
MIRT593402 Znrf3 zinc and ring finger 3 2 2
MIRT593411 Slc6a6 solute carrier family 6 (neurotransmitter transporter, taurine), member 6 2 2
MIRT593503 Enpp4 ectonucleotide pyrophosphatase/phosphodiesterase 4 2 2
MIRT594179 Vdr vitamin D (1,25-dihydroxyvitamin D3) receptor 2 2
MIRT594723 Zfp931 zinc finger protein 931 2 2
MIRT594918 Gm14326 predicted gene 14326 2 2
MIRT594954 Fblim1 filamin binding LIM protein 1 2 2
MIRT594996 Tmem245 transmembrane protein 245 1 1
MIRT595717 Calcr calcitonin receptor 2 2
MIRT601211 Arhgef9 CDC42 guanine nucleotide exchange factor (GEF) 9 2 2
MIRT604988 Fsd1l fibronectin type III and SPRY domain containing 1-like 2 2
MIRT605314 Zfp92 zinc finger protein 92 2 2
MIRT605458 St18 suppression of tumorigenicity 18 2 2
MIRT605538 Pstpip2 proline-serine-threonine phosphatase-interacting protein 2 2 2
MIRT605601 Nbeal1 neurobeachin like 1 2 2
MIRT605769 Epas1 endothelial PAS domain protein 1 2 2
MIRT605937 Sema6a sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A 2 2
MIRT605986 Mylk4 myosin light chain kinase family, member 4 2 2
MIRT606188 Slc5a8 solute carrier family 5 (iodide transporter), member 8 2 2
MIRT606238 Rab3c RAB3C, member RAS oncogene family 2 2
MIRT606736 Tacr1 tachykinin receptor 1 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-466e-5p Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 up-regulated

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