pre-miRNA Information
pre-miRNA mmu-mir-186   
Genomic Coordinates chr3: 157544279 - 157544349
Synonyms Mirn186, mmu-mir-186, Mir186
Description Mus musculus miR-186 stem-loop
Comment The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies .
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-186-5p
Sequence 7| CAAAGAAUUCUCCUUUUGGGCU |28
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Intu   
Synonyms 9230116I04Rik, 9430087H23Rik, Pdzd6, Pdzk6, mKIAA1284
Description inturned planar cell polarity protein
Transcript NM_175515   
Expression
Putative miRNA Targets on Intu
3'UTR of Intu
(miRNA target sites are highlighted)
>Intu|NM_175515|3'UTR
   1 CTGTGTTTGTAAGATGCAAAGCAACACACCGATGGACCACAGGACATTGTCAGAATGACAGAAATCATCATACAGAGATA
  81 AGGCTCAGTACATTCACTATTCACTATAGTACTCGTTAAATACTGAGATGCAATCCTATTGGGTTGGTCCATCAGTTCAT
 161 GTGTGGTATTATACAAGACTCTGAGCTGGTACTTGATCAAAATATAGACACAAGATTGTTAATTTATTGCCATTTTGGTA
 241 TACCTTGCTAATTTATTGAATAGTTTTAAATAATGTGAATTCTTTAATAAAAAATTAATATAGATGGTTTTTTAGGGTTC
 321 ATATTTTAATCATTGTTTGTTTTCATAAAACCCAATACTGAATGACTGAAACCTGGTGAAGAAGTCATGACTTGAAGAGA
 401 TGGCAAGGTTCTGTAGCTGGTTAGTGCTGTGTTGTGAGCAAGCGCTTGCATCAGTTAGACCTCAGAGTATGTGTAGACTA
 481 ATCACATTATTATTGCATTAGGATTTTGAAACACCCTGTTAAGAAAAATGTGTTGTTTGCTTATTGATCAGTATATAAAT
 561 TTACAAAACCTATGTTCTAGTTTCTTAGCAAATCAAAAGCCTGTGCACTGATTTAAGTATTTTAGTAGATACCTCACTAC
 641 AGAGGCAGAGGGAGAGGATGGCAGTTAAAGTTAATGTATATTTATTTGATTCTCTTTATTGTTTATTTGGATTAGGATAC
 721 ACATCTGCAATCATCTGTAAACTCCCCCTGCTTTCTTTCTGGTTCACATACATTTATGTCAGCTCTATCGAATCATATTA
 801 AAGGCACTGAAGTGGGGGCTGGATCAATGGCTCAGAGGTCAGGAACAAGTACCTCATTGGCAGAGACCTGAGTTCAGCTC
 881 CCAGCACCCACATTAGGTAGCTCAAACCCCCTGTAACTCCAGGGACTCCTTTGGCTTTTGCCAGGTGCCCACACACACAA
 961 TTAGAAAATAAAAATCCTTTTTAAAACACTGGGGGTCGGGGTGGTGGTAAGGAACAAGCTAAAGCTATTGTATGTAACTT
1041 TTTCTATAAAATACTAATTTCTGTCTTACAAATCAGCATCAATCTGCACTGGGTTTTTCCAAAGGAGAGGGGTTGCTTTC
1121 CCCGACTGTGGCTAATGTGAGCTTCAGCGGTACAGAAAGCTGACAAGACAGATTTTCTTTGGCTGTGAGAATTTGGTGTG
1201 TTGAAAAGTATAGTAAATGCTAAATTTATTATTGCCATCTACCCAGGTGGACATCCCAGAGGCTCAATATCCCCCTACAT
1281 TTTACAGTCCTGGGTAATGTCTCTTTCAGGGGTTATATATGTTGGAAGGCAAGCTATCTATCTTAGTCAGGGTTTCTATT
1361 CCTGCACAAACATCATGGCCAAGAAGCAAGTTGGGGAGGAAAGGGTTTATTTGGCTTACACTTCCATACTGCTGTTCATC
1441 ACCAAGGAAGTCAAGACTGGAACTCAAGCAGGTCAGGAAGCAGGAGCTGATGCAGAGGCCATGGAGGGATGTTCTTTACT
1521 GGCTTGCCTCACCTGGCTTGCTCAGCCTGCTTTCTTATAGAACCAAGACTACCAGCCCTGAGATGGTCCCACCCACAAGG
1601 GGCCTTTCCCCCTTGATCACTAATTGAGAAAATGCCTGACAGTTGTATCTCATGGAGGCATTTCCTCAACTGAAGCTCCT
1681 TCCTCTGTGATAACTCCAGCTGTGTCAAGTTGACACAAAACTAGCCAGTACACTATCTAAAGCCAATCTAATGTTTGTCC
1761 CTACATTTCCCCTCCCTCAAACACTAACCCTCCAGCTTAAAACTAGAAAGCATACTAGTGCACCCCTGAGCCATTCGGTC
1841 TTATAAAACTACTGTTGCCTTGGCCATCAGTGTAAGATTCTCATCAAGGCATAGGTGACAGATACCAGCTGTTTACATCA
1921 CTGGTGACTTCTGGGCCTTCATTGCGGCTTTCAAGTCAGATAAGAAAATTGTGCACCTTACAATGTAGAGAACAGTTTGA
2001 AATATCTCCCCACGTAACTGAGTCAATTAGTTAAGACTCACTTCTGTCTTAGACGGCCCGTGTGCCGATGTGTGTTCCAG
2081 CATTTCTAAAAGTGTACTCATTAAATGTCTCAAGTTTTTGGATATAGCCTCAAATCCCGAGCTGGTTCTAGTGCTGATCA
2161 TTATGATGAATGTAGCTGTTGATGACCTCAGGTGTTCAGGCAGAACAAACTAAGACTTGGGGTTTTAATAAAGGGAACTT
2241 TTTTCCATTTAAATTTTGATCTGTTCATGCTCTCTTCATGTCAGATAGCCATGCACACATTATTCAAAATGTGGTGTGAA
2321 GGACAAGCACAGCACAGATGTTAAAGATGAGCCACTGGCAGTGCAGTGAGTGTGCATCATAATTCCATACTGAAGAGACA
2401 CAGCATTTTTAAGGAAAGAATATTGTCTTCCTTTCCATTTCCAAGTGAACACTGAAGGCAAACATAGCTGACCATGCCCG
2481 TAAATAAATTCAGACTCACATCTGAAAGTCAATCAGTCTGACTGTTGGACAGTAGAGTCCCTGTCTGTCTTGTTTTCAGG
2561 GAGAGGACAAAATATCAAGTGCAGATCCCATAAGGATGGGCTTCAGAGAAACCTCCTTGAGTCAGGTTTTATTCACATCC
2641 TGGTAGTCGTGTTCTAAAGAACATTACTGTACGAAAGTAAAATGCATGGTCTGCCTAACATTCTTAGAACTGTTATGTCT
2721 GGCTTGGTGGCACATGCCTAGAATCCAGTGTTCAGGAAGCTGAGGCAGAGGGCAGGCTGGGTTCTATCTGTAACAAAAAT
2801 AAAACCACAAGTGCTTTCTTCCTACTACCTCACGTTTCTTAGCCCCAAAGAAACTCCAGACTCTTGCTTTTTCTTTCCCT
2881 CTTCTCTCTCTTCTCTCTTTCCTTTGTTTCGGTCTTGTCAGAGAACTGTACCTAGGAGGGTACGGTAGGCAGTGTATCAC
2961 TTTTTGTGCCAAGGGACAATAGATGATGATACATACATACCTAACAAAATGGTGATTGTAAAATAATCTCTTAAATATTT
3041 CTGACACAAATCGAAAAACCGTGAAACTAGTCTACTAAGTTTTGTCTGCTTTAACCATTGTTCGTTTCCATATGCTCTCC
3121 CTAAACCATGGTTGTCATACATTGTCAATTATGAAGCATAAGCTGTGGCTACCATTTATCATCATTAAGTGGCCCCAGCT
3201 TGAGAAACAAAAGGGTACAGTTAAATCATGCCCGTTACTGCATAACATGACAGCAACGATCTGAGAAAATTACTTTGTTG
3281 TATAGTTTTGAAAATAAGGATTTATATTTTGGATTTTTTTAAATGGTAAGTTCTATTAAAACCTGAACCTACACACCAAA
3361 CTGTATGAACTAACTTAATTATGAAGAGTTTTATTTCTGTATGTAATTAAAGCTATTTTTCTACATTATCCAGAGTTTTA
3441 AATAAAAATGTTTTGGAGGTT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' ucGGGUU--UUCCUCUUAAGAAAc 5'
            :::||  || | ||||||||| 
Target 5' gtTTTAAATAATGTGAATTCTTTa 3'
263 - 286 162.00 -6.90
2
miRNA  3' ucgGGU-UUUCCUCUUAAGAAAc 5'
             ||| ::|||:  :|||||| 
Target 5' aggCCATGGAGGGATGTTCTTTa 3'
1496 - 1518 143.00 -16.30
3
miRNA  3' ucgggUUUUCCU----------CUUAAGAAAc 5'
               | |||||          |:|||:||| 
Target 5' tgaaaATAAGGATTTATATTTTGGATTTTTTt 3'
3289 - 3320 131.00 -6.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
46 mmu-miR-186-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577608 Stxbp4 syntaxin binding protein 4 1 1
MIRT577884 Qpct glutaminyl-peptide cyclotransferase (glutaminyl cyclase) 1 1
MIRT578137 Noc3l NOC3 like DNA replication regulator 1 1
MIRT578249 Mnat1 menage a trois 1 1 1
MIRT579147 Ces2g carboxylesterase 2G 1 1
MIRT579219 Ccdc138 coiled-coil domain containing 138 1 1
MIRT579578 4933427D06Rik RIKEN cDNA 4933427D06 gene 1 1
MIRT580348 Tmem33 transmembrane protein 33 1 1
MIRT580592 Tbl1xr1 transducin (beta)-like 1X-linked receptor 1 1 1
MIRT580668 Stk32a serine/threonine kinase 32A 1 1
MIRT580744 Srek1 splicing regulatory glutamine/lysine-rich protein 1 1 1
MIRT580779 Sp6 trans-acting transcription factor 6 1 1
MIRT580847 Sltm SAFB-like, transcription modulator 1 1
MIRT580944 Slc26a5 solute carrier family 26, member 5 1 1
MIRT581214 Samd4 sterile alpha motif domain containing 4 1 1
MIRT581276 Rprd2 regulation of nuclear pre-mRNA domain containing 2 1 1
MIRT581520 Ptprb protein tyrosine phosphatase, receptor type, B 1 1
MIRT582203 Nfia nuclear factor I/A 1 1
MIRT582893 Ildr2 immunoglobulin-like domain containing receptor 2 1 1
MIRT582996 Id4 inhibitor of DNA binding 4 1 1
MIRT583079 Hnrnpa3 heterogeneous nuclear ribonucleoprotein A3 1 1
MIRT583125 Heca hdc homolog, cell cycle regulator 1 1
MIRT583739 Epm2aip1 EPM2A (laforin) interacting protein 1 1 1
MIRT584127 Crispld2 cysteine-rich secretory protein LCCL domain containing 2 1 1
MIRT584166 Cpox coproporphyrinogen oxidase 1 1
MIRT584531 C230081A13Rik pseudopodium-enriched atypical kinase 1 1 1
MIRT584646 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 1 1
MIRT587978 Armc1 armadillo repeat containing 1 1 1
MIRT588832 Sp1 trans-acting transcription factor 1 1 2
MIRT590758 Acbd5 acyl-Coenzyme A binding domain containing 5 1 1
MIRT593146 Mmp12 matrix metallopeptidase 12 1 4
MIRT593495 Gjc3 gap junction protein, gamma 3 1 1
MIRT594880 Lrig2 leucine-rich repeats and immunoglobulin-like domains 2 1 1
MIRT594898 Ifi204 interferon activated gene 204 1 1
MIRT594932 Gdi2 guanosine diphosphate (GDP) dissociation inhibitor 2 1 1
MIRT594945 Fmnl2 formin-like 2 1 1
MIRT595197 Emc3 ER membrane protein complex subunit 3 1 1
MIRT595343 Frmd4a FERM domain containing 4A 1 1
MIRT595402 Arsk arylsulfatase K 1 1
MIRT595430 A630033H20Rik RIKEN cDNA A630033H20 gene 1 1
MIRT595947 Nelfa negative elongation factor complex member A, Whsc2 1 1
MIRT596153 Opn5 opsin 5 1 1
MIRT601390 Utp23 UTP23 small subunit processome component 1 1
MIRT602153 Intu inturned planar cell polarity protein 1 1
MIRT602577 Colec12 collectin sub-family member 12 1 1
MIRT604817 Nr5a2 nuclear receptor subfamily 5, group A, member 2 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-186 Gemcitabine approved 60750 Northern blot Mz-ChA-1 human cholangiocarcinoma cell lines 16762633 2006 down-regulated
miR-186 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-186 1,2,6-Tri-O-galloyl-beta-D-glucopyranose NULL NULL Microarray HepG2 hepatocarcinoma cells. 22506400 2011 down-regulated
miR-186 Ginsenoside Rh2 NULL 119307 Microarray NSCLC cell line A549 23152132 2013 up-regulated
miR-186 Nicotine approved 89594 Microarray Rat adrenal pheochromocytoma PC12 cell 18845019 2009 down-regulated

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