pre-miRNA Information | |
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pre-miRNA | mmu-mir-186 |
Genomic Coordinates | chr3: 157544279 - 157544349 |
Synonyms | Mirn186, mmu-mir-186, Mir186 |
Description | Mus musculus miR-186 stem-loop |
Comment | The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies . |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
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Mature miRNA | mmu-miR-186-5p |
Sequence | 7| CAAAGAAUUCUCCUUUUGGGCU |28 |
Evidence | Experimental |
Experiments | Cloned |
Putative Targets |
Gene Information | |||||||||||||||||||||
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Gene Symbol | Intu | ||||||||||||||||||||
Synonyms | 9230116I04Rik, 9430087H23Rik, Pdzd6, Pdzk6, mKIAA1284 | ||||||||||||||||||||
Description | inturned planar cell polarity protein | ||||||||||||||||||||
Transcript | NM_175515 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on Intu | |||||||||||||||||||||
3'UTR of Intu (miRNA target sites are highlighted) |
>Intu|NM_175515|3'UTR 1 CTGTGTTTGTAAGATGCAAAGCAACACACCGATGGACCACAGGACATTGTCAGAATGACAGAAATCATCATACAGAGATA 81 AGGCTCAGTACATTCACTATTCACTATAGTACTCGTTAAATACTGAGATGCAATCCTATTGGGTTGGTCCATCAGTTCAT 161 GTGTGGTATTATACAAGACTCTGAGCTGGTACTTGATCAAAATATAGACACAAGATTGTTAATTTATTGCCATTTTGGTA 241 TACCTTGCTAATTTATTGAATAGTTTTAAATAATGTGAATTCTTTAATAAAAAATTAATATAGATGGTTTTTTAGGGTTC 321 ATATTTTAATCATTGTTTGTTTTCATAAAACCCAATACTGAATGACTGAAACCTGGTGAAGAAGTCATGACTTGAAGAGA 401 TGGCAAGGTTCTGTAGCTGGTTAGTGCTGTGTTGTGAGCAAGCGCTTGCATCAGTTAGACCTCAGAGTATGTGTAGACTA 481 ATCACATTATTATTGCATTAGGATTTTGAAACACCCTGTTAAGAAAAATGTGTTGTTTGCTTATTGATCAGTATATAAAT 561 TTACAAAACCTATGTTCTAGTTTCTTAGCAAATCAAAAGCCTGTGCACTGATTTAAGTATTTTAGTAGATACCTCACTAC 641 AGAGGCAGAGGGAGAGGATGGCAGTTAAAGTTAATGTATATTTATTTGATTCTCTTTATTGTTTATTTGGATTAGGATAC 721 ACATCTGCAATCATCTGTAAACTCCCCCTGCTTTCTTTCTGGTTCACATACATTTATGTCAGCTCTATCGAATCATATTA 801 AAGGCACTGAAGTGGGGGCTGGATCAATGGCTCAGAGGTCAGGAACAAGTACCTCATTGGCAGAGACCTGAGTTCAGCTC 881 CCAGCACCCACATTAGGTAGCTCAAACCCCCTGTAACTCCAGGGACTCCTTTGGCTTTTGCCAGGTGCCCACACACACAA 961 TTAGAAAATAAAAATCCTTTTTAAAACACTGGGGGTCGGGGTGGTGGTAAGGAACAAGCTAAAGCTATTGTATGTAACTT 1041 TTTCTATAAAATACTAATTTCTGTCTTACAAATCAGCATCAATCTGCACTGGGTTTTTCCAAAGGAGAGGGGTTGCTTTC 1121 CCCGACTGTGGCTAATGTGAGCTTCAGCGGTACAGAAAGCTGACAAGACAGATTTTCTTTGGCTGTGAGAATTTGGTGTG 1201 TTGAAAAGTATAGTAAATGCTAAATTTATTATTGCCATCTACCCAGGTGGACATCCCAGAGGCTCAATATCCCCCTACAT 1281 TTTACAGTCCTGGGTAATGTCTCTTTCAGGGGTTATATATGTTGGAAGGCAAGCTATCTATCTTAGTCAGGGTTTCTATT 1361 CCTGCACAAACATCATGGCCAAGAAGCAAGTTGGGGAGGAAAGGGTTTATTTGGCTTACACTTCCATACTGCTGTTCATC 1441 ACCAAGGAAGTCAAGACTGGAACTCAAGCAGGTCAGGAAGCAGGAGCTGATGCAGAGGCCATGGAGGGATGTTCTTTACT 1521 GGCTTGCCTCACCTGGCTTGCTCAGCCTGCTTTCTTATAGAACCAAGACTACCAGCCCTGAGATGGTCCCACCCACAAGG 1601 GGCCTTTCCCCCTTGATCACTAATTGAGAAAATGCCTGACAGTTGTATCTCATGGAGGCATTTCCTCAACTGAAGCTCCT 1681 TCCTCTGTGATAACTCCAGCTGTGTCAAGTTGACACAAAACTAGCCAGTACACTATCTAAAGCCAATCTAATGTTTGTCC 1761 CTACATTTCCCCTCCCTCAAACACTAACCCTCCAGCTTAAAACTAGAAAGCATACTAGTGCACCCCTGAGCCATTCGGTC 1841 TTATAAAACTACTGTTGCCTTGGCCATCAGTGTAAGATTCTCATCAAGGCATAGGTGACAGATACCAGCTGTTTACATCA 1921 CTGGTGACTTCTGGGCCTTCATTGCGGCTTTCAAGTCAGATAAGAAAATTGTGCACCTTACAATGTAGAGAACAGTTTGA 2001 AATATCTCCCCACGTAACTGAGTCAATTAGTTAAGACTCACTTCTGTCTTAGACGGCCCGTGTGCCGATGTGTGTTCCAG 2081 CATTTCTAAAAGTGTACTCATTAAATGTCTCAAGTTTTTGGATATAGCCTCAAATCCCGAGCTGGTTCTAGTGCTGATCA 2161 TTATGATGAATGTAGCTGTTGATGACCTCAGGTGTTCAGGCAGAACAAACTAAGACTTGGGGTTTTAATAAAGGGAACTT 2241 TTTTCCATTTAAATTTTGATCTGTTCATGCTCTCTTCATGTCAGATAGCCATGCACACATTATTCAAAATGTGGTGTGAA 2321 GGACAAGCACAGCACAGATGTTAAAGATGAGCCACTGGCAGTGCAGTGAGTGTGCATCATAATTCCATACTGAAGAGACA 2401 CAGCATTTTTAAGGAAAGAATATTGTCTTCCTTTCCATTTCCAAGTGAACACTGAAGGCAAACATAGCTGACCATGCCCG 2481 TAAATAAATTCAGACTCACATCTGAAAGTCAATCAGTCTGACTGTTGGACAGTAGAGTCCCTGTCTGTCTTGTTTTCAGG 2561 GAGAGGACAAAATATCAAGTGCAGATCCCATAAGGATGGGCTTCAGAGAAACCTCCTTGAGTCAGGTTTTATTCACATCC 2641 TGGTAGTCGTGTTCTAAAGAACATTACTGTACGAAAGTAAAATGCATGGTCTGCCTAACATTCTTAGAACTGTTATGTCT 2721 GGCTTGGTGGCACATGCCTAGAATCCAGTGTTCAGGAAGCTGAGGCAGAGGGCAGGCTGGGTTCTATCTGTAACAAAAAT 2801 AAAACCACAAGTGCTTTCTTCCTACTACCTCACGTTTCTTAGCCCCAAAGAAACTCCAGACTCTTGCTTTTTCTTTCCCT 2881 CTTCTCTCTCTTCTCTCTTTCCTTTGTTTCGGTCTTGTCAGAGAACTGTACCTAGGAGGGTACGGTAGGCAGTGTATCAC 2961 TTTTTGTGCCAAGGGACAATAGATGATGATACATACATACCTAACAAAATGGTGATTGTAAAATAATCTCTTAAATATTT 3041 CTGACACAAATCGAAAAACCGTGAAACTAGTCTACTAAGTTTTGTCTGCTTTAACCATTGTTCGTTTCCATATGCTCTCC 3121 CTAAACCATGGTTGTCATACATTGTCAATTATGAAGCATAAGCTGTGGCTACCATTTATCATCATTAAGTGGCCCCAGCT 3201 TGAGAAACAAAAGGGTACAGTTAAATCATGCCCGTTACTGCATAACATGACAGCAACGATCTGAGAAAATTACTTTGTTG 3281 TATAGTTTTGAAAATAAGGATTTATATTTTGGATTTTTTTAAATGGTAAGTTCTATTAAAACCTGAACCTACACACCAAA 3361 CTGTATGAACTAACTTAATTATGAAGAGTTTTATTTCTGTATGTAATTAAAGCTATTTTTCTACATTATCCAGAGTTTTA 3441 AATAAAAATGTTTTGGAGGTT Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
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miRNA-target interactions (Predicted by miRanda) |
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Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | mESCs |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM622573. RNA binding protein: AGO2. Condition:KO1
... - Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology. |
Article |
- Leung AK; Young AG; Bhutkar A; Zheng GX; et al. - Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
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46 mmu-miR-186-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT577608 | Stxbp4 | syntaxin binding protein 4 | ![]() |
1 | 1 | |||||||
MIRT577884 | Qpct | glutaminyl-peptide cyclotransferase (glutaminyl cyclase) | ![]() |
1 | 1 | |||||||
MIRT578137 | Noc3l | NOC3 like DNA replication regulator | ![]() |
1 | 1 | |||||||
MIRT578249 | Mnat1 | menage a trois 1 | ![]() |
1 | 1 | |||||||
MIRT579147 | Ces2g | carboxylesterase 2G | ![]() |
1 | 1 | |||||||
MIRT579219 | Ccdc138 | coiled-coil domain containing 138 | ![]() |
1 | 1 | |||||||
MIRT579578 | 4933427D06Rik | RIKEN cDNA 4933427D06 gene | ![]() |
1 | 1 | |||||||
MIRT580348 | Tmem33 | transmembrane protein 33 | ![]() |
1 | 1 | |||||||
MIRT580592 | Tbl1xr1 | transducin (beta)-like 1X-linked receptor 1 | ![]() |
1 | 1 | |||||||
MIRT580668 | Stk32a | serine/threonine kinase 32A | ![]() |
1 | 1 | |||||||
MIRT580744 | Srek1 | splicing regulatory glutamine/lysine-rich protein 1 | ![]() |
1 | 1 | |||||||
MIRT580779 | Sp6 | trans-acting transcription factor 6 | ![]() |
1 | 1 | |||||||
MIRT580847 | Sltm | SAFB-like, transcription modulator | ![]() |
1 | 1 | |||||||
MIRT580944 | Slc26a5 | solute carrier family 26, member 5 | ![]() |
1 | 1 | |||||||
MIRT581214 | Samd4 | sterile alpha motif domain containing 4 | ![]() |
1 | 1 | |||||||
MIRT581276 | Rprd2 | regulation of nuclear pre-mRNA domain containing 2 | ![]() |
1 | 1 | |||||||
MIRT581520 | Ptprb | protein tyrosine phosphatase, receptor type, B | ![]() |
1 | 1 | |||||||
MIRT582203 | Nfia | nuclear factor I/A | ![]() |
1 | 1 | |||||||
MIRT582893 | Ildr2 | immunoglobulin-like domain containing receptor 2 | ![]() |
1 | 1 | |||||||
MIRT582996 | Id4 | inhibitor of DNA binding 4 | ![]() |
1 | 1 | |||||||
MIRT583079 | Hnrnpa3 | heterogeneous nuclear ribonucleoprotein A3 | ![]() |
1 | 1 | |||||||
MIRT583125 | Heca | hdc homolog, cell cycle regulator | ![]() |
1 | 1 | |||||||
MIRT583739 | Epm2aip1 | EPM2A (laforin) interacting protein 1 | ![]() |
1 | 1 | |||||||
MIRT584127 | Crispld2 | cysteine-rich secretory protein LCCL domain containing 2 | ![]() |
1 | 1 | |||||||
MIRT584166 | Cpox | coproporphyrinogen oxidase | ![]() |
1 | 1 | |||||||
MIRT584531 | C230081A13Rik | pseudopodium-enriched atypical kinase 1 | ![]() |
1 | 1 | |||||||
MIRT584646 | Bach2 | BTB and CNC homology, basic leucine zipper transcription factor 2 | ![]() |
1 | 1 | |||||||
MIRT587978 | Armc1 | armadillo repeat containing 1 | ![]() |
1 | 1 | |||||||
MIRT588832 | Sp1 | trans-acting transcription factor 1 | ![]() |
1 | 2 | |||||||
MIRT590758 | Acbd5 | acyl-Coenzyme A binding domain containing 5 | ![]() |
1 | 1 | |||||||
MIRT593146 | Mmp12 | matrix metallopeptidase 12 | ![]() |
1 | 4 | |||||||
MIRT593495 | Gjc3 | gap junction protein, gamma 3 | ![]() |
1 | 1 | |||||||
MIRT594880 | Lrig2 | leucine-rich repeats and immunoglobulin-like domains 2 | ![]() |
1 | 1 | |||||||
MIRT594898 | Ifi204 | interferon activated gene 204 | ![]() |
1 | 1 | |||||||
MIRT594932 | Gdi2 | guanosine diphosphate (GDP) dissociation inhibitor 2 | ![]() |
1 | 1 | |||||||
MIRT594945 | Fmnl2 | formin-like 2 | ![]() |
1 | 1 | |||||||
MIRT595197 | Emc3 | ER membrane protein complex subunit 3 | ![]() |
1 | 1 | |||||||
MIRT595343 | Frmd4a | FERM domain containing 4A | ![]() |
1 | 1 | |||||||
MIRT595402 | Arsk | arylsulfatase K | ![]() |
1 | 1 | |||||||
MIRT595430 | A630033H20Rik | RIKEN cDNA A630033H20 gene | ![]() |
1 | 1 | |||||||
MIRT595947 | Nelfa | negative elongation factor complex member A, Whsc2 | ![]() |
1 | 1 | |||||||
MIRT596153 | Opn5 | opsin 5 | ![]() |
1 | 1 | |||||||
MIRT601390 | Utp23 | UTP23 small subunit processome component | ![]() |
1 | 1 | |||||||
MIRT602153 | Intu | inturned planar cell polarity protein | ![]() |
1 | 1 | |||||||
MIRT602577 | Colec12 | collectin sub-family member 12 | ![]() |
1 | 1 | |||||||
MIRT604817 | Nr5a2 | nuclear receptor subfamily 5, group A, member 2 | ![]() |
1 | 1 |
miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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