pre-miRNA Information | |
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pre-miRNA | mmu-mir-30a |
Genomic Coordinates | chr1: 23272269 - 23272339 |
Synonyms | Mirn30a, mmu-mir-30a, Mir30a |
Description | Mus musculus miR-30a stem-loop |
Comment | Landgraf et al. confirm that the 5' mature miRNA product is the predominant one . |
RNA Secondary Structure | ![]() |
Mature miRNA Information | |
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Mature miRNA | mmu-miR-30a-5p |
Sequence | 6| UGUAAACAUCCUCGACUGGAAG |27 |
Evidence | Experimental |
Experiments | Cloned |
Putative Targets |
Biomarker Information |
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Gene Information | |
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Gene Symbol | Trp53rk |
Experimental Support 1 for Functional miRNA-Target Interaction | |
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miRNA:Target | ---- |
Validation Method |
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Conditions | mESCs |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A
HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B
HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2
... - Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology. |
Article |
- Leung AK; Young AG; Bhutkar A; Zheng GX; et al. - Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
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57 mmu-miR-30a-5p Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
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Strong evidence | Less strong evidence | |||||||||||
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MIRT003714 | Ak1 | adenylate kinase 1 | ![]() |
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4 | 1 | ||||
MIRT003715 | Tnrc6a | trinucleotide repeat containing 6a | ![]() |
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4 | 1 | ||||
MIRT003716 | Abcb1b | ATP-binding cassette, sub-family B (MDR/TAP), member 1B | ![]() |
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3 | 1 | |||||
MIRT003717 | Inhba | inhibin beta-A | ![]() |
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3 | 1 | |||||
MIRT003718 | Egfr | epidermal growth factor receptor | ![]() |
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4 | 2 | ||||
MIRT004770 | Bdnf | brain derived neurotrophic factor | ![]() |
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5 | 2 | |||
MIRT007045 | Snai1 | snail family zinc finger 1 | ![]() |
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2 | 1 | ||||||
MIRT054524 | Ccne2 | cyclin E2 | ![]() |
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2 | 1 | ||||||
MIRT054525 | Celsr3 | cadherin, EGF LAG seven-pass G-type receptor 3 | ![]() |
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2 | 1 | ||||||
MIRT054526 | Mdm2 | transformed mouse 3T3 cell double minute 2 | ![]() |
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2 | 1 | ||||||
MIRT054527 | Mtdh | metadherin | ![]() |
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3 | 2 | |||||
MIRT054528 | Smad1 | SMAD family member 1 | ![]() |
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2 | 1 | ||||||
MIRT054529 | Twf1 | twinfilin actin binding protein 1 | ![]() |
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2 | 1 | ||||||
MIRT054530 | Timp3 | tissue inhibitor of metalloproteinase 3 | ![]() |
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2 | 1 | ||||||
MIRT577999 | Pgr | progesterone receptor | ![]() |
1 | 1 | |||||||
MIRT578189 | Narg2 | interactor of little elongation complex ELL subunit 2 | ![]() |
1 | 1 | |||||||
MIRT578280 | Mesdc2 | mesoderm development LRP chaperone | ![]() |
1 | 1 | |||||||
MIRT578462 | Ints12 | integrator complex subunit 12 | ![]() |
1 | 1 | |||||||
MIRT578487 | Il18r1 | interleukin 18 receptor 1 | ![]() |
1 | 2 | |||||||
MIRT580036 | Wdr89 | WD repeat domain 89 | ![]() |
1 | 3 | |||||||
MIRT580042 | Wdr44 | WD repeat domain 44 | ![]() |
1 | 1 | |||||||
MIRT580299 | Tomm70a | translocase of outer mitochondrial membrane 70 homolog A (yeast) | ![]() |
1 | 1 | |||||||
MIRT581001 | Six4 | sine oculis-related homeobox 4 | ![]() |
1 | 1 | |||||||
MIRT581268 | Rrad | Ras-related associated with diabetes | ![]() |
1 | 1 | |||||||
MIRT582213 | Nfatc3 | nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 | ![]() |
1 | 1 | |||||||
MIRT582448 | Mgea5 | meningioma expressed antigen 5 (hyaluronidase) | ![]() |
1 | 1 | |||||||
MIRT583057 | Hoxb3 | homeobox B3 | ![]() |
1 | 1 | |||||||
MIRT583627 | Strip1 | striatin interacting protein 1 | ![]() |
1 | 1 | |||||||
MIRT583684 | Amer1 | APC membrane recruitment 1 | ![]() |
1 | 1 | |||||||
MIRT583926 | Dpy19l1 | dpy-19-like 1 (C. elegans) | ![]() |
1 | 1 | |||||||
MIRT584694 | Cep170b | centrosomal protein 170B | ![]() |
1 | 3 | |||||||
MIRT585022 | Gskip | GSK3B interacting protein | ![]() |
1 | 1 | |||||||
MIRT585237 | Zfp446 | zinc finger protein 446 | ![]() |
1 | 1 | |||||||
MIRT586099 | Reep4 | receptor accessory protein 4 | ![]() |
1 | 2 | |||||||
MIRT587711 | Cd300a | CD300A molecule | ![]() |
1 | 2 | |||||||
MIRT588338 | Zfp711 | zinc finger protein 711 | ![]() |
1 | 1 | |||||||
MIRT588632 | Tnrc6b | trinucleotide repeat containing 6b | ![]() |
1 | 3 | |||||||
MIRT589298 | Pigh | phosphatidylinositol glycan anchor biosynthesis, class H | ![]() |
1 | 1 | |||||||
MIRT590161 | Eno2 | enolase 2, gamma neuronal | ![]() |
1 | 1 | |||||||
MIRT592638 | Lpar3 | lysophosphatidic acid receptor 3 | ![]() |
1 | 2 | |||||||
MIRT593645 | Tbc1d5 | TBC1 domain family, member 5 | ![]() |
1 | 1 | |||||||
MIRT595042 | Camk4 | calcium/calmodulin-dependent protein kinase IV | ![]() |
1 | 1 | |||||||
MIRT595217 | Slc16a1 | solute carrier family 16 (monocarboxylic acid transporters), member 1 | ![]() |
1 | 1 | |||||||
MIRT595451 | Ptrhd1 | peptidyl-tRNA hydrolase domain containing 1 | ![]() |
1 | 1 | |||||||
MIRT595826 | Fam227a | family with sequence similarity 227, member A | ![]() |
1 | 1 | |||||||
MIRT596025 | Cdk13 | cyclin-dependent kinase 13 | ![]() |
1 | 1 | |||||||
MIRT596356 | Serpine1 | serine (or cysteine) peptidase inhibitor, clade E, member 1 | ![]() |
1 | 1 | |||||||
MIRT596419 | Ing1 | inhibitor of growth family, member 1 | ![]() |
1 | 1 | |||||||
MIRT596513 | Zfp9 | zinc finger protein 9 | ![]() |
1 | 1 | |||||||
MIRT599308 | Csf1 | colony stimulating factor 1 (macrophage) | ![]() |
1 | 1 | |||||||
MIRT603175 | Trp53rk | transformation related protein 53 regulating kinase B | ![]() |
1 | 1 | |||||||
MIRT731329 | IL21R | interleukin 21 receptor | ![]() |
1 | 1 | |||||||
MIRT733494 | Gfra1 | glial cell line derived neurotrophic factor family receptor alpha 1 | ![]() |
1 | 0 | |||||||
MIRT735519 | SOCS1 | suppressor of cytokine signaling 1 | ![]() |
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2 | 0 | ||||||
MIRT755305 | Runx2 | runt related transcription factor 2 | 2 | 1 | ||||||||
MIRT755548 | SOCS3 | suppressor of cytokine signaling 3 | 3 | 1 | ||||||||
MIRT755880 | NCAM1 | neural cell adhesion molecule 1 | 1 | 1 |
miRNA-Drug Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||
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