miRTarBase - #MIRT603333


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pre-miRNA Information
pre-miRNA mmu-mir-743b   
Genomic Coordinates chrX: 66777256 - 66777332
Description Mus musculus miR-743b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-743b-3p
Sequence 46| GAAAGACAUCAUGCUGAAUAGA |67
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Slc35d2   
Synonyms 5730408I21Rik, SQV7L, UGTrel8, hfrc, mUGTrel8
Description solute carrier family 35, member D2
Transcript NM_001001321   
Expression
Putative miRNA Targets on Slc35d2
3'UTR of Slc35d2
(miRNA target sites are highlighted)		 
>Slc35d2|NM_001001321|3'UTR
   1 GCTGAAGACAGAGTTGCAGGCCTAGGCTGGAAAGAGCAGCCTCAGCAGGAGTGGGTAGACCAGAGGTCCCACACATGGGG
  81 CAGTCACCTGCTTGGTCAGAGCACAAAAAGGAATGTCTTCGAAATACAAAGAAAGCTACCTCACACGTCACTGCACAATG
 161 AGCCACGAGGAACCCACGACTCACAGTACAAAACCTTCCAACTAAGTGAATTTGCTAAAGTCATCCACAGGGCCCTCACT
 241 ATGTGCTGGGGAAGATGTTTCAAGGGTGACTTTGCTTATCAGCACTGTGGCCTTAATTTTAAAGGCCTCATCCAAGGAAA
 321 AAGGGAAAACTTTTTGTTGTTGAACCATCTCTTTGTCTTTGTAAGAGTCATTCAGTTTTGAATGTGTTGGTATTTTTTTT
 401 CCAGGTCATTACAGTGAAATAAAGTCCTGTCTATATATGCCGCACAGTGTGATATTACAGTATGATAGAAAATAGTTAGT
 481 TATTCAGTGTCCAGGAATATTCCTGGGGTTGAGGTGTATAGAGTGCTCACTGTACTCAGGAGACAAATGAGTTTGATCTC
 561 TGCACTCATATAAAAAGTCAGGCATGGAGCATATCAATTATCTCAGCTTTGGGTAGCAGAGACAGACATGTCCTGAGACT
 641 CTGGCTTGCCAGCCAGTCTTAAATGGCAAGCATCAGGTTCAGTGAGAGACACTGTCTCAAAGAATAGGATGGAGAGTGAC
 721 TGAGGAAGACACCCATCATTAGCCTCCGGTCTCCACATACACATGCACACACATACATACATGTGTGCACACATTACTCC
 801 ATCTTTTTTTTTAAAAAGATTTTATTTATTTATTATATGTAAGTACACTGTAGCTGTCTTTAGATCTCATTACGGGTGGT
 881 TGTGAGCCACCATGTGGTTGCTGGGACTTGAACTCAGGACCTCCAGAAGAGCAGTCATTGCTCTTAACTGCTGAGCCATC
 961 TCACCAGCCCACATTACTCCATCTTGAAGGAACAACAAGATAGCCTTTTAAGGACAAGTGTTGGAAAAACTTTGCAAAAA
1041 CCCAAAGCCATCAGTTCATCTCCGTAGTCTTAGACATTTGAATGTGTGGTCCCTGGTTGGGTGGCATTGCTGGAGGAAAT
1121 GTGTCCCTGGGGTGGGCTCTGAGGTTTCAAAGTCTCCATCCATTTCAGGTTTGCTTGCTCAGTTTCTTGTTTGTGGTTTG
1201 ACGAGCTCTCAGCTGTTCCTGCCACACTGATCTCTCTGGAACTGTAAGCCCAAATAAACTCTTCTATACATT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' agaUAAGUCGUA-CUACAGAAAg 5'
             :| || ::| | ||||||| 
Target 5' taaGTACACTGTAGCTGTCTTTa 3'
 840 - 862 151.00 -9.30
2 
miRNA  3' agAUAA-GUCGUA---CUACAGAAAg 5'
            |:||  | |||     ||||||| 
Target 5' gtTGTTGAACCATCTCTTTGTCTTTg 3'
 336 - 361 144.00 -6.00
3 
miRNA  3' agauaagUCGUACUACAGAAag 5'
                 || |  |||||||  
Target 5' cacaaaaAGGA--ATGTCTTcg 3'
 102 - 121 127.00 -5.50
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
CLIP-seq Support 1 for dataset GSM4751761
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 2
Location of target site NM_001001321 | 3UTR | AGAUCUCAUUACGGGUGG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM622570
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1A
Location of target site NM_001001321 | 3UTR | AGAUCUCAUUACGGGUGGUUGUGAGCCACCAUGUGGUUGCUGGGACUUGAACUCAGGACCUCCAGAAGAGCAGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 3 for dataset GSM622571
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1B
Location of target site NM_001001321 | 3UTR | AGAUCUCAUUACGGGUGGUUGUGAGCCACCAUGUGGUUGCUGGGACUUGAACUCAGGACCUCCAGAAGAGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 4 for dataset GSM622572
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT2
Location of target site NM_001001321 | 3UTR | AGAUCUCAUUACGGGUGGUUGUGAGCCACCAUGUGGUUGCUGGGACUUGAACUCAGGACCUCCAGAAGAGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
101 mmu-miR-743b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577961 Pm20d2 peptidase M20 domain containing 2 2 2
MIRT578387 Klra2 killer cell lectin-like receptor, subfamily A, member 2 2 6
MIRT579831 Zfhx3 zinc finger homeobox 3 2 8
MIRT580616 Syp synaptophysin 2 2
MIRT580731 Srrm4 serine/arginine repetitive matrix 4 2 2
MIRT581440 Rasa2 RAS p21 protein activator 2 2 2
MIRT582031 Pappa pregnancy-associated plasma protein A 2 2
MIRT582678 Lin28b lin-28 homolog B (C. elegans) 2 8
MIRT582831 Itih5 inter-alpha (globulin) inhibitor H5 2 2
MIRT583084 Hlcs holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) 2 8
MIRT583879 Dusp18 dual specificity phosphatase 18 2 6
MIRT583900 Dstyk dual serine/threonine and tyrosine protein kinase 2 4
MIRT585980 Sfxn4 sideroflexin 4 2 4
MIRT590345 Cntn2 contactin 2 2 6
MIRT591765 Ppm1f protein phosphatase 1F (PP2C domain containing) 2 4
MIRT592938 Astn2 astrotactin 2 2 2
MIRT593579 Zfp709 zinc finger protein 709 2 2
MIRT593675 Slc5a3 solute carrier family 5 (inositol transporters), member 3 2 2
MIRT593750 Peg10 paternally expressed 10 2 2
MIRT593771 Nmnat3 nicotinamide nucleotide adenylyltransferase 3 2 2
MIRT593825 Krit1 KRIT1, ankyrin repeat containing 2 2
MIRT593859 Gm5460 predicted gene 5460 2 2
MIRT593871 Galnt10 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 2 2
MIRT593891 Elovl5 ELOVL family member 5, elongation of long chain fatty acids (yeast) 2 2
MIRT593935 Cxcl3 chemokine (C-X-C motif) ligand 3 2 2
MIRT593937 Cttnbp2nl CTTNBP2 N-terminal like 2 2
MIRT594001 Aptx aprataxin 2 2
MIRT594019 Tmem241 transmembrane protein 241 1 1
MIRT594730 Zfp931 zinc finger protein 931 2 2
MIRT594766 Tmlhe trimethyllysine hydroxylase, epsilon 2 2
MIRT594836 Opa3 optic atrophy 3 2 2
MIRT594873 Magt1 magnesium transporter 1 2 2
MIRT594927 Gm14326 predicted gene 14326 2 2
MIRT594941 Fosl2 fos-like antigen 2 2 2
MIRT594980 Eda2r ectodysplasin A2 receptor 2 2
MIRT595014 Cpd carboxypeptidase D 2 2
MIRT595076 Yae1d1 Yae1 domain containing 1 1 1
MIRT595132 Lrrc4c leucine rich repeat containing 4C 2 2
MIRT595148 Epha7 Eph receptor A7 2 2
MIRT595169 Zpbp zona pellucida binding protein 2 2
MIRT595180 Tsn translin 2 2
MIRT595185 Trim25 tripartite motif-containing 25 2 2
MIRT595187 Top2a topoisomerase (DNA) II alpha 2 2
MIRT595193 Tmtc3 transmembrane and tetratricopeptide repeat containing 3 2 2
MIRT595199 Tbc1d2b TBC1 domain family, member 2B 2 2
MIRT595204 St6gal1 beta galactoside alpha 2,6 sialyltransferase 1 2 2
MIRT595210 Snx27 sorting nexin family member 27 2 2
MIRT595226 Serinc1 serine incorporator 1 2 2
MIRT595229 Sema4d sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D 2 2
MIRT595232 Sec24a Sec24 related gene family, member A (S. cerevisiae) 2 2
MIRT595235 Scn3a sodium channel, voltage-gated, type III, alpha 2 2
MIRT595242 Rc3h1 RING CCCH (C3H) domains 1 2 2
MIRT595255 Ppm1k protein phosphatase 1K (PP2C domain containing) 2 2
MIRT595259 Plxna4 plexin A4 2 2
MIRT595264 Phf3 PHD finger protein 3 2 2
MIRT595271 Nxph1 neurexophilin 1 2 2
MIRT595273 Npas3 neuronal PAS domain protein 3 2 2
MIRT595298 Kcnv1 potassium channel, subfamily V, member 1 2 2
MIRT595317 Gna14 guanine nucleotide binding protein, alpha 14 2 2
MIRT595321 Gm10318 predicted gene 10318 2 2
MIRT595328 Galnt4 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 2 2
MIRT595347 Frem2 Fras1 related extracellular matrix protein 2 2 2
MIRT595375 Epb4.1l4a erythrocyte membrane protein band 4.1 like 4a 2 2
MIRT595380 Dusp11 dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) 2 2
MIRT595383 Dek DEK oncogene (DNA binding) 2 2
MIRT595385 Dcun1d1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) 2 2
MIRT595391 Cetn3 centrin 3 2 2
MIRT595394 Cd8a CD8 antigen, alpha chain 2 2
MIRT595398 Asprv1 aspartic peptidase, retroviral-like 1 2 2
MIRT595403 Arid2 AT rich interactive domain 2 (ARID, RFX-like) 2 2
MIRT595414 Ankrd16 ankyrin repeat domain 16 2 2
MIRT595416 Anapc11 anaphase promoting complex subunit 11 2 2
MIRT595421 Akna AT-hook transcription factor 2 2
MIRT595438 9930013L23Rik cell migration inducing protein, hyaluronan binding 2 2
MIRT595441 9030617O03Rik D-glutamate cyclase 2 2
MIRT595459 Zswim6 zinc finger SWIM-type containing 6 2 2
MIRT595465 Tspan13 tetraspanin 13 2 2
MIRT595467 Tmem41b transmembrane protein 41B 2 2
MIRT595472 Sv2b synaptic vesicle glycoprotein 2 b 2 2
MIRT595475 Sgk3 serum/glucocorticoid regulated kinase 3 2 2
MIRT595480 Scaf11 SR-related CTD-associated factor 11 2 2
MIRT595485 Phox2b paired-like homeobox 2b 2 2
MIRT595490 Pgp phosphoglycolate phosphatase 2 2
MIRT595494 Nr1d2 nuclear receptor subfamily 1, group D, member 2 2 2
MIRT595511 Lrig1 leucine-rich repeats and immunoglobulin-like domains 1 2 2
MIRT595524 Grm2 glutamate receptor, metabotropic 2 2 2
MIRT595528 Foxn3 forkhead box N3 2 2
MIRT595536 Fign fidgetin 2 2
MIRT595546 Celf2 CUGBP, Elav-like family member 2 2 2
MIRT595553 Cd28 CD28 antigen 2 2
MIRT595840 Sumf1 sulfatase modifying factor 1 2 2
MIRT595953 Rab6a RAB6A, member RAS oncogene family 1 1
MIRT598445 Lix1 limb and CNS expressed 1 2 2
MIRT600750 Iws1 IWS1, SUPT6 interacting protein 2 2
MIRT601150 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 2 2
MIRT602248 Grm1 glutamate receptor, metabotropic 1 2 2
MIRT603333 Slc35d2 solute carrier family 35, member D2 2 2
MIRT603399 Serpina1a serine (or cysteine) peptidase inhibitor, clade A, member 1A 2 2
MIRT604755 Rad51d RAD51 paralog D 1 1
MIRT605865 Atpbd4 diphthamine biosynthesis 6 2 2
MIRT606713 0610030E20Rik RIKEN cDNA 0610030E20 gene 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-743b Propranolol approved 4946 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-743b-3p (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 down-regulated

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