miRTarBase - #MIRT603947


—
pre-miRNA Information
pre-miRNA mmu-mir-452   
Genomic Coordinates chrX: 72262224 - 72262308
Synonyms Mirn452, mmu-mir-452, Mir452
Description Mus musculus miR-452 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-452-3p
Sequence 53| UCAGUCUCAUCUGCAAAGAGGU |74
Evidence Experimental
Experiments Illumina
Putative Targets

Gene Information
Gene Symbol Grap2   
Synonyms GRAP-2, GRB2L, GRBLG, GRID, Gads, GrbX, Grf40, GrpL, Mona, P38
Description GRB2-related adaptor protein 2
Transcript NM_010815   
Expression
Putative miRNA Targets on Grap2
3'UTR of Grap2
(miRNA target sites are highlighted)		 
>Grap2|NM_010815|3'UTR
   1 GTCGTACTGCAAGGCTTAGGGGCTTTTCGTCCAAAGCCGCCTGCAAGACAGAGAGGGGCGAGGGGGTAAAGCTGAACTTT
  81 ATAATGACACATACATGCATACATGTGCATGTGTGCCCGAGTACACAAAACATGTATATGTATGCCGATGGCTACACACG
 161 ACATTTTATAATAGTAATTTATTGGCAGGCAGGTTGGCTCGTTGATCAGCATGAGAAGGAACTTGGGTGGCGAGGGGGCC
 241 GTTTTGCCAGCTCTTCTCTGCCTGAGAGGGCAGTGGGGAGCTGGGAGCAGGGCGGGGAAATGACCTTTTCCAACCCTCAA
 321 ACTTTCACCGTTTCCTTCCTGGCTTGGGAATTTTCCAAGAGAGCAGGCAGCTCAGCAGAGATCACACCCCAGGGATGGAT
 401 GAACAGGGCAAGGCTGGGCTTGGCGAGGCACGTTAGGTAGTTCACCTGGCTCTGTCCACTTACAGGGTAGCTCCCCTCTT
 481 TTGCATAGAGCAGGCGCTGCGCCCTGAGAATTAAGGTGTAGCAGATGCAGGGCTTCAGGCACCAGGCAAGTCTGAGCAGG
 561 TCGCCAGCAAGGGTGTGGTCGGGAGGCTCCTCTTGGTGTGGGAGAGACACGCAGGCTCAGGCCTAGCCGATGTCTGGCTA
 641 GGACTTTTACTTTCCACCAGAGACTGTCCCTTGGGCAATCCTCTTCCCCTACGGCTGACAGGAGAGTTAGAACGGAGCGA
 721 TGCCACAGAGGTTCTCCACAGGCCCTTGTGGCTTACTTGAATGTAACTTGTTTCCTCGGCTGCAGAGAAAGCTGCAGCCT
 801 AGATTCTTGGAAGTGCTTGGAGAAGCCCTGGCGGAGAATGTGGGATGTGTCAGCAGCCTCACTCTGTGGTTACAAGAGTG
 881 TCTCTGTGGAGCTCAATTGGGTTTTGCTCCTGATTTTCTGTGGTTGAGGACATCTTTGCCAGTGGACGAAAGGGAAGCCA
 961 AGAGTGCTGACTGTGGATCGACTGATAAATTGACAGACTGACTCTAACCCTGACAGGAGCTTTCACGGAAATGGGTTTCC
1041 TCTTCTGTTTGAGCGCTAGGCCTTCCCCTATCCTCCCTCCACCTGGGAAGAGGAAGCCCACTGCCACTGTCCCTCTGTCA
1121 CCTTAAACTAGAATTGTGTGTCCTGGGGAATGGAGCACAGAGAAGCCTGGCTGGGTGGGGAGGGGGACTCTGGAGAGGAA
1201 GCCCAGTGCTTTTGGGCAGCCTGGGAGTAAAGATGTCAGAACAGAAGGTACTTACAAGGGTGTTGGTGTAGTCGTGTGTG
1281 TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTCTAAGATAGGAAAGGAAGAGCTGGGTGAAAGGGGTGGTAA
1361 GCATATGTGATGGGAGGGGGGCTGTCTCCTTGTCCCCTCCTGCTTTAGGGAGGGACTGGAGGGCATCTGTTAGTGAGGGA
1441 GACAAAAGAACAGTCTGTGACTCAGGAGTCACTGCAGTGTGGCACATACTCTGTCCCTTGTGACTCTGATTCAACAATCT
1521 TCTGATCCCTAGGTTCCCTTTAGAGAGAAAGAGAGGGACCAGGAGGGAAGAGATCATAGCCTGGAGGCTCAGGGATGCAG
1601 CTGCAGCCCTGGGACAGGAAAGCAGAGACAGTGTCAGAAGATGGGTGGCAGGTGGGAGACAGGGAGACTGTGGGGGGTGG
1681 GGGTGGGGTAGTATCAATTGGGGCAGCAAGGGGAGACATGGGCAGGTGGTACAGAAAGAACTCTTACCCCTCCCCCGGAG
1761 AAGGTAGGAGCCCCCCCCCTAACACCCCCCCCCCAGCAGGTGAGGAAAGAGTGCATCAGGAGGAGGTGAAGGGCAGAGAA
1841 GGCGCCAAGGAGGAAAAATGAGGCCGGAAGCTGAGGCAAAGTGGGCCCAAGCTTGGTAGTAGGTGATGTGAGACATTACA
1921 GTAAATAAAAGCCAAAAGTGGTGTTTGGTTTTACAGTATTTGCAAACCATATGCTTTCCAACAGCCCACAAACTTCATCC
2001 ATTTGGCTGATTCTGTGGGAGGAGCAGCCCTGGCTGAGGCAGAGATGACTAGCCTGGTTCTGGTCTAGTTACTATGCCTT
2081 GAAATACTCTATGAGAATACAGTAATAAAGTTTTGCCAGCCTGTATCTGGTGCTCATGCTGCACAGCCCTAGGAGAAGGG
2161 GGTACTCCTCACCTTGGGGCAGCAAAACCCCTAGAACAGGCATCCTGTTCTAGCAGGAGCCCTGGTGGGAGAAGGTAGGA
2241 GGAATTCTTTATTGAGGTATCCTCATTAGCAGGGTTCGATTCGAAAGGGAACAGGAGAGTCACCCCAAACCATGAAGGGA
2321 GACCAAGGACTCCCAGGGGCTGAGCCTAGGAGGCCAGCACCAAACTGTCTACCTGGGGCTCAGATCTCTTTGGATTCTGC
2401 TACTGCTTCCTGGGAAGTAGGTAAGGAAGAGAGCATGAGCCATCATACCCCCCAACTCTCTCTCTCTCTCTCTCTGGCTG
2481 CACAGCCAACTGCAAGTACACGGTCTCTGATTTTGGCCGACACACCATAGGTGCGACTTCATGACATCTGAGCAGAGAAC
2561 TTCTAAGAAGAGCCAGTTCCTGTTGACCAATCAGAGTTTCCTCCCCACTTGCTGAAAAATCACTTCATTTCGAATATCCA
2641 GCAGCTACCATGGCCCACTCCCCATTACATGGTCTCAGTCTTTCCAAGATCTCAAGTATGTGAGCATTGTCTGGGGGCCG
2721 TGTTAAAATGCTGGCTCTGACTCAGCCTTATATCTATCTCTAACAAGCTCCCAGGTGCTTTGATCAGCAAGGGCATCGGT
2801 CTCACAAATCTAATTGGATTCTTTTTCTGGGGATAGTCAGCCACTGCTCTCTGTTGCTAGCGAATAAGTCACTTTGGCTT
2881 TGGGACAGGCACAACTCGTTACTGTCTTTATGACACCATTCCTGTTTGGCGGCCAGTGACTTCTTTTTTACATCTTCATC
2961 GGGGATCAGAAATTTTCCAAGTGACAGGCATTTGGCTGGAGACTCACACAGGAATTCCCTACTAGAAATCACTTTAACAG
3041 GAGCAGAAGCTGTGGAAGGTGCAGGAAGACTTCTCAGGAAATCCTGAAAGAGAAAAATCAATGTCCATCACTTTGGGCTC
3121 TGGGCAAGGCAGGGAATATCACTAGATCTCAGCTCACAGCCCACACAAGGGACAGACCGTCCTGGTCAAAATAATTGCAT
3201 GACACAATTGTTTTGTCTAGTTTCAATGTGACTATCTGTAAGACAAGAGTGTGAAAAAAATCTACCACTGCAATTCTTTA
3281 AAACAAATTTATTTTTGTTATTGTTTTTCAAGTCCTTCCACTTCATCTCCCAGCCGGCGGCATCTTTCTGTCTCCCATAG
3361 CAACTGCACTATCTTCTGTACTTGTTGCCCTGCATTCCTTCCTGTGCCTGGTACACTCTCCACTCAGACACCAAGGCAAT
3441 CTTTGGACAGACAAACCCAATCACACCACTTTTCTTCACAGGCATTTATCCTTGAACCTAAAATCCCAGCCCGGTCTTGC
3521 AAGGTCTGGCCCCTTCGGGATCATCTCTCCAACTTCCTCTTGCTTTCATACCCCTTCCCCACTGTCCTTCCTTCAGCCTC
3601 CAGATGGCTAAGAACCTTCCGAAGGGGGAGTCATAACTGTGGGTCCCCCCAGGATGCACATCCTGTGCTTTCCAGCTGAC
3681 TGCTGTTCAGATCTGGGTTCACACGTCACTTCCTCTCATCACCCCCATGTGGAATAAAATACATCTTTAGATTACCTCTT
3761 GAGTCCCATGGTTTTCCTGAACACCATGCACACAGATGGGGGCACTAGATCGAGTGGTGTGACCATTAGACTGTATGCTT
3841 CTTGGGGTGTGTGTGGGGTCATTGTATTTTGTAGATACTTTATATATTCATGGCACATGGGGAAGGCTGTCAGTAGATAT
3921 GTGTCTAATGAATGATATTGTGTTTCTCTAAGGTGAAAACTCAGGTATAAAATGAGAAAAGGGTGAGACAAGATGCTCTA
4001 ACGAGAAGGTGAAACACTCTTACAAGGAAACTCATTAAGATAGGAAGTAACCAACTCAAAATACCTTCAGGAAGTCCCTG
4081 AAACTGACTAGATTAACTAGGCCCCTTTCTCCTCTGTAATATATAAGCAGCAAAGACAGCTGTGATTCTTAGAAGATTCT
4161 CAAACAAAACATCCCAGAATAATCAAAGACCAGCCAAAAGCCTGAAAAAAAAAAAAAAAAAAAAAAAAAGAAGAGACCAG
4241 CTGAACTGCCTG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' uggaGAAACGUCUACUCUGACu 5'
              |||| ||   ||||||| 
Target 5' tttaCTTTCCACCAGAGACTGt 3'
 646 - 667 158.00 -13.40
2 
miRNA  3' uggagaaacGUCUACUCUGACu 5'
                   |||: ||||||| 
Target 5' ggtgggagaCAGG-GAGACTGt 3'
 1651 - 1671 148.00 -15.30
3 
miRNA  3' ugGA-GAAACGUCUACUCUGACu 5'
            || ||||  :|| |:||||| 
Target 5' tcCTGCTTTAGGGA-GGGACTGg 3'
 1398 - 1419 143.00 -18.70
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' uggagaaacgucuaCUCUGACu 5'
                        ||||||| 
Target 5' -------------gGAGACUGu 3'
 		1 - 9
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
CLIP-seq Support 1 for dataset GSM622571
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1B
Location of target site NM_010815 | 3UTR | GGGAGACUGUGGGGGGUGGGGGU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM622572
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT2
Location of target site NM_010815 | 3UTR | GGAGACUGUGGGGGGUGGGGGUGGGGUAGUAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
26 mmu-miR-452-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT429003 Nfib nuclear factor I/B 2 2
MIRT577016 Vmp1 vacuole membrane protein 1 2 8
MIRT580097 Usp54 ubiquitin specific peptidase 54 2 2
MIRT581145 Senp1 SUMO1/sentrin specific peptidase 1 2 2
MIRT581215 Samd4 sterile alpha motif domain containing 4 2 2
MIRT582710 Lcorl ligand dependent nuclear receptor corepressor-like 2 6
MIRT583742 Ephb3 Eph receptor B3 2 10
MIRT583790 Elk4 ELK4, member of ETS oncogene family 2 2
MIRT584103 Csnk1e casein kinase 1, epsilon 2 6
MIRT587976 Armc1 armadillo repeat containing 1 2 2
MIRT590630 Arl5a ADP-ribosylation factor-like 5A 2 2
MIRT591969 Bbx bobby sox HMG box containing 2 2
MIRT591998 Wrn Werner syndrome RecQ like helicase 2 2
MIRT594081 Sema6d sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D 2 2
MIRT594218 Ube2k ubiquitin-conjugating enzyme E2K 2 2
MIRT594859 Nacc1 nucleus accumbens associated 1, BEN and BTB (POZ) domain containing 2 2
MIRT595550 Celf2 CUGBP, Elav-like family member 2 2 2
MIRT595570 Mtf1 metal response element binding transcription factor 1 2 2
MIRT596982 Tango6 transport and golgi organization 6 1 1
MIRT599081 Eid2b EP300 interacting inhibitor of differentiation 2B 2 2
MIRT599176 Dhdh dihydrodiol dehydrogenase (dimeric) 2 2
MIRT600982 Denr density-regulated protein 2 2
MIRT602199 Hlcs holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) 2 2
MIRT603280 Stambp STAM binding protein 2 2
MIRT603947 Grap2 GRB2-related adaptor protein 2 2 2
MIRT606257 Polr1e polymerase (RNA) I polypeptide E 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-452 Formaldehyde NULL 712 Microarray Human lung epithelial cells (A549) 21147603 2011 down-regulated
miR-452 Phenethyl isothiocyanate(PEITC) NULL 16741 Microarray neonatal mice liver 20145010 2010 up-regulated
miR-452 Tert-butyl hydroperoxide (t-BHP) NULL 6410 Microarray mouse auditory cells 20510889 2010 down-regulated
miR-452 Reversine NULL 210332 Microarray C2C12 myoblast cells 24513286 2014 down-regulated

Error report submission