miRTarBase - #MIRT604052


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pre-miRNA Information
pre-miRNA mmu-mir-325   
Genomic Coordinates chrX: 105379082 - 105379179
Synonyms Mirn325, mmu-mir-325, Mir325
Description Mus musculus miR-325 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-325-3p
Sequence 54| UUUAUUGAGCACCUCCUAUCAA |75
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Esf1   
Synonyms 2610101J03Rik, ABTAP, AW545818, C79684
Description ESF1 nucleolar pre-rRNA processing protein homolog
Transcript NM_001081090   
Expression
Putative miRNA Targets on Esf1
3'UTR of Esf1
(miRNA target sites are highlighted)		 
>Esf1|NM_001081090|3'UTR
   1 CTGCTCTCGCCTCAAGTGTACAGAAACAGTGTAAAGGATAGTGGGAAGAACGCCACCTTTCTGAAATAGGAGAAATACCC
  81 TTTTTTGACCATTGTAAACTTGTTCTGCTTTGGGGCTGGAGAGATGGCTCAGCAATAAAGAATACTGGTGCTTTTGCAGA
 161 GCATCCGTGTTTGGTTCCCAGCTCTCACAAGGCAGCTCACAGCCATATGTAATTTGAGAGACCCGGTGCCTTTTCCTGGC
 241 CTCCATGGGCATGGCTGGCCCATAGTGTTTGCATAAACATAGTTACAGGCAAAATATTCACACACAGAAAATTTTAAAGT
 321 AAAATTTAGAAAAAATGTTCCCCTTATGTAATTGTTTACCTAATTGTAAATACTTCATGTTTTATACTGTGTATTCATAG
 401 ACTTGCCATAATGTAATTATATATACTATAGAATTCTTAAGAATTTTATATTATATGAAGTTCATTTCATATAAGTACAG
 481 TTATTCAAGCTTCCTAAGATGCTATTTACAATCTATGTAAAATAGTGTCAATTTTTTCTCTGTAAAGAATAAAAACAAGT
 561 TGAAAATATGTATATGTAGTACCTTAATCTCTGAAGGCAGGAAGCAGATCTCTGAAGCTAGTAAAATAATTTATTTTTCT
 641 AGTGTGAAAGATGGAAGCTGTGAACTTTCTTTCTGTGCTTAGTAATTTGCATGTAGAACTAATTTATAAATAAAAAGTGT
 721 GAAATGAAATT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' aacuauccucCACGAGUUAUUu 5'
                    |||||:|:||| 
Target 5' ctttctttctGTGCTTAGTAAt 3'
 665 - 686 128.00 -10.70
2 
miRNA  3' aacUAUCCUCCACG---AGU-UAUUu 5'
             ||| || || |   ||| |||| 
Target 5' attATATGAAGTTCATTTCATATAAg 3'
 450 - 475 127.00 -7.70
3 
miRNA  3' aacuauccuccacgaGUUAUUu 5'
                         |||||| 
Target 5' ggagagatggctcagCAATAAa 3'
 118 - 139 120.00 -10.20
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
27 mmu-miR-325-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT054600 Arc activity regulated cytoskeletal-associated protein 3 1
MIRT577829 Rinl Ras and Rab interactor-like 1 1
MIRT577905 Prr11 proline rich 11 1 1
MIRT578380 Krtap6-1 keratin associated protein 6-1 1 1
MIRT578550 Hsd17b1 hydroxysteroid (17-beta) dehydrogenase 1 1 1
MIRT578770 Gemin8 gem nuclear organelle associated protein 8 1 1
MIRT580288 Trhr thyrotropin releasing hormone receptor 1 1
MIRT581518 Ptprd protein tyrosine phosphatase, receptor type, D 1 1
MIRT582779 Kif1c kinesin family member 1C 1 1
MIRT585752 Stard6 StAR-related lipid transfer (START) domain containing 6 1 1
MIRT587310 Ephx3 epoxide hydrolase 3 1 4
MIRT588735 Taok3 TAO kinase 3 1 1
MIRT592550 Mup7 major urinary protein 7 1 1
MIRT592592 Mup13 major urinary protein 13 1 1
MIRT594207 Wdr12 WD repeat domain 12 1 1
MIRT594462 Epyc epiphycan 1 1
MIRT594693 Atp11b ATPase, class VI, type 11B 1 1
MIRT594929 Gjb2 gap junction protein, beta 2 1 1
MIRT594934 Frem3 Fras1 related extracellular matrix protein 3 1 1
MIRT595784 Hlf hepatic leukemia factor 1 1
MIRT595846 Set SET nuclear oncogene 1 1
MIRT595897 Cd69 CD69 antigen 1 1
MIRT603010 Klk8 kallikrein related-peptidase 8 1 1
MIRT603579 Ppm1k protein phosphatase 1K (PP2C domain containing) 1 1
MIRT604052 Esf1 ESF1 nucleolar pre-rRNA processing protein homolog 1 1
MIRT736870 GHRHR growth hormone releasing hormone receptor 2 0
MIRT756335 Gsdmd gasdermin D 3 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-325 Mistletoe lectin-I NULL NULL Microarray colorectal cancer cells CLY cells 20955366 2011 down-regulated
miR-325 Mistletoe lectin-I NULL NULL Microarray colorectal cancer cells HT-29 cells 20955366 2011 down-regulated
miR-325 Cisplatin approved 84093 Microarray CNE cells 22614822 2012 up-regulated
miR-325 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) NULL 16078949 Microarray hepatocytes 23107762 2013 up-regulated
miR-325-3p Urocortin 2 NULL 56843276 Quantitative real-time PCR anterior pituitary cells 22252941 2012 up-regulated

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