miRTarBase - #MIRT604581


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pre-miRNA Information
pre-miRNA mmu-mir-466h   
Genomic Coordinates chr2: 10514891 - 10514971
Description Mus musculus miR-466h stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-466h-5p
Sequence 11| UGUGUGCAUGUGCUUGUGUGUA |32
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Trim71   
Synonyms 2610206G21Rik, AL022943, Gm1127, Lin41, lin-41, mLin41, mlin-41
Description tripartite motif-containing 71
Transcript NM_001042503   
Expression
Putative miRNA Targets on Trim71
3'UTR of Trim71
(miRNA target sites are highlighted)		 
>Trim71|NM_001042503|3'UTR
   1 TTGTGTCTTCTGGGGTTTTCTGTGTTTAGGGTGTGCATGTGTGTGTCTCTCATTTTTGAATTTCAAAGAAGAAATCGTCT
  81 CAGGGATATTTCTTTTTCTTTTTCCCCCCCTTTATTTTTATTATTTTTTTTTTTTTAAGAACAAAAGTACAACATTGCCT
 161 AAGTCCTACCTCAGCTTAAATTTTTTTTTTTTTTTTTTTTTTACAGATGAATGTAATTCTCCTCTGTGCAGGGCTTGAGC
 241 CTGTGAAGTGATAATTTCTATCTACCTCAACTCTTTGCATTTCCCTCTCTGGCAAGCCTCTTGGCTCACATCCTCTTCCC
 321 TCCTGAAGGCCTAGGAGGCACAGGACAGCGGTCCTGCAACCTTGAGGGCACTGGAAGCATATGGTGGGGTGCATGCATTT
 401 TGTAGATTGAGCCAAGGAAACCCAAAAACTACTAAGTAAAAAAGAGAAGTATAAGATGTTGGAAAGATAGGATTTAAAAT
 481 TCATAATTGTAGTGTATTTGTGTTCTTGAGAATACTGTGTTTATGTGGGGTTAGGTTGTGTGTGGTTTGCTCTTTTCTTT
 561 TTTATCGATGCAACAGGGCTTTTCTCTGTTTACCTCAGTGTGTCTGACATCTCATCCCTAGGAGATATGGCTGTGGCTTG
 641 CTAGCCTAGAAAAGCAGACCATTCATATTTTCGGGTGTCTCATCTTTGTATCTGGTAAAGAAGGGTGGGTGTGTGGGAGT
 721 GTGTGTGTGCACACGCGTGCATGTTTGTGTTTCAAAGCCATCTTTGCTTGAGGAGAAAAGCTGGACTTGACACAAGACAT
 801 CCCAAGCAAATTTCTTCCCAGTTTGCTAAGAAACTCGATTTAGCTCAGGACTCTAGGTTGAAAGTGTTCGCAAAGGTTAC
 881 GTGTCTGTGTTTTTGTTGTTTGTTATAAAGACTTAGGTATAAGAAAATAAGTTAACCTTGTTCTCAGAGAGAGAGGGGCT
 961 TTTCAGATGTGGACCAGGGTGCCACAGAAGTGGGGGGTGCTGGAGAAGTGAGGCACCCCCACATCAGGGACTGTTCAGGA
1041 GGGCGGGGGACCTCGTCTCTATGTGTGAGGGTGTGGTAGGCAAAGGGATAGCTTTGATTTGAGAAGCACAATGTTTTTTC
1121 CTGCTTTGGAAGTTCAGGGAATGTAGAAGATTGTCTGACTGGTGGGAGCACCTCACTCCCTAACCCTTGGCTCAGGTCTG
1201 TTTCTCAGGAACCTTCCCTAAGTCTGAGTGGAGGCAGACCTCAAGGTTAACACAAAGCCCCGTTCCTCCCCCAAAGGCCT
1281 TTGAATGTAAAGGATTTACAAACCAAACCACCCAACAGGAAGTGTAGGACACAGTATATGTCTTTTTTTTTTTTTAATTT
1361 TATTTTATTTAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' auguguguuCGUGUACGUGUGu 5'
                   |::::||||||| 
Target 5' tgggagtgtGTGTGTGCACACg 3'
 714 - 735 149.00 -15.30
2 
miRNA  3' auGUG-UGUUCGUGU--------ACGUGUGu 5'
            ||| : |||||:|        ||||::| 
Target 5' ggCACTGGAAGCATATGGTGGGGTGCATGCa 3'
 367 - 397 122.00 -14.90
3 
miRNA  3' augUGUGUUCG---UGUAC-GUGUgu 5'
             | | ||||   || || ||||  
Target 5' aggAGAAAAGCTGGACTTGACACAag 3'
 771 - 796 107.00 -7.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622572. RNA binding protein: AGO2. Condition:WT2 ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' auGUGUGUUCGUGUACGUGUGu 5'
            :::: :||::::||::::| 
Target 5' ugUGUGGGAGUGUGUGUGUGC- 3'
 		19 - 39
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
CLIP-seq Support 1 for dataset GSM4751763
Method / RBP HITS-CLIP / AGO
Cell line / Condition adipose tissue / RNA sequencing of eWAT 4
Location of target site NM_001042503 | 3UTR | GGGAGUGUGUGUGUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Accession Series GSE142677
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM622572
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT2
Location of target site NM_001042503 | 3UTR | CUGGUAAAGAAGGGUGGGUGUGUGGGAGUGUGUGUGUGC
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
87 mmu-miR-466h-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577339 Zfp157 zinc finger protein 157 2 2
MIRT577488 Tns4 tensin 4 2 6
MIRT578067 Oxsm 3-oxoacyl-ACP synthase, mitochondrial 2 2
MIRT578343 Ltf lactotransferrin 2 2
MIRT578743 Gm4841 predicted gene 4841 2 4
MIRT578822 Fermt1 fermitin family member 1 2 2
MIRT579127 Chrnd cholinergic receptor, nicotinic, delta polypeptide 2 2
MIRT579135 Chrna1 cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle) 2 2
MIRT579305 BC021785 major facilitator superfamily domain containing 4B5 2 4
MIRT579417 Agtrap angiotensin II, type I receptor-associated protein 2 2
MIRT579732 Zfp92 zinc finger protein 92 2 4
MIRT579978 Wrn Werner syndrome RecQ like helicase 2 4
MIRT581035 Sim1 single-minded homolog 1 (Drosophila) 2 4
MIRT581532 Pten phosphatase and tensin homolog 2 2
MIRT582312 Nab1 Ngfi-A binding protein 1 2 4
MIRT582843 Itga9 integrin alpha 9 2 4
MIRT582976 Igf2 insulin-like growth factor 2 2 4
MIRT583470 Foxk1 forkhead box K1 2 4
MIRT584662 B4galt6 UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 6 2 4
MIRT585220 Zfp488 zinc finger protein 488 2 4
MIRT585299 Zfp26 zinc finger protein 26 2 2
MIRT585311 Zfp248 zinc finger protein 248 2 4
MIRT585835 Slc6a17 solute carrier family 6 (neurotransmitter transporter), member 17 2 4
MIRT586077 Rhobtb1 Rho-related BTB domain containing 1 2 2
MIRT586329 Pgm5 phosphoglucomutase 5 2 4
MIRT587381 Dzip3 DAZ interacting protein 3, zinc finger 2 2
MIRT587611 Cml2 N-acetyltransferase 8 (GCN5-related) family member 2 2 2
MIRT587631 Clec7a C-type lectin domain family 7, member a 2 8
MIRT587725 Cd28 CD28 antigen 2 2
MIRT587758 Cd200r1 CD200 receptor 1 2 2
MIRT588012 Akap7 A kinase (PRKA) anchor protein 7 2 2
MIRT588296 1700019G17Rik N-acetyltransferase 8 (GCN5-related) family member 4 2 2
MIRT588555 Uhrf1bp1l UHRF1 (ICBP90) binding protein 1-like 2 2
MIRT588689 Tet2 tet methylcytosine dioxygenase 2 2 2
MIRT588893 Slc1a2 solute carrier family 1 (glial high affinity glutamate transporter), member 2 2 4
MIRT589021 Rnf11 ring finger protein 11 2 2
MIRT589101 Rasal2 RAS protein activator like 2 2 4
MIRT590106 Etv3 ets variant 3 2 2
MIRT590170 Elovl6 ELOVL family member 6, elongation of long chain fatty acids (yeast) 2 4
MIRT590929 Tacr2 tachykinin receptor 2 2 2
MIRT591061 Ptpre protein tyrosine phosphatase, receptor type, E 2 4
MIRT591144 Nsun3 NOL1/NOP2/Sun domain family member 3 2 2
MIRT591210 Mdm2 transformed mouse 3T3 cell double minute 2 2 2
MIRT591429 Car10 carbonic anhydrase 10 2 4
MIRT591502 Acot2 acyl-CoA thioesterase 2 2 4
MIRT591557 Zfp449 zinc finger protein 449 2 2
MIRT591632 Ubtf upstream binding transcription factor, RNA polymerase I 2 4
MIRT591952 Ceacam1 carcinoembryonic antigen-related cell adhesion molecule 1 2 2
MIRT591973 Ap1ar adaptor-related protein complex 1 associated regulatory protein 2 2
MIRT592040 Tnfrsf13c tumor necrosis factor receptor superfamily, member 13c 2 2
MIRT592091 Smo smoothened, frizzled class receptor 2 2
MIRT592109 Slc25a12 solute carrier family 25 (mitochondrial carrier, Aralar), member 12 2 2
MIRT592174 Oxtr oxytocin receptor 2 2
MIRT592217 Map3k7 mitogen-activated protein kinase kinase kinase 7 2 2
MIRT592224 Magee2 melanoma antigen, family E, 2 2 6
MIRT592250 Lcp2 lymphocyte cytosolic protein 2 2 4
MIRT592267 Kcnj16 potassium inwardly-rectifying channel, subfamily J, member 16 2 2
MIRT592390 Trp53i11 transformation related protein 53 inducible protein 11 2 6
MIRT592420 Stxbp5l syntaxin binding protein 5-like 2 2
MIRT592441 Snx12 sorting nexin 12 2 2
MIRT592515 Npr3 natriuretic peptide receptor 3 2 2
MIRT592626 Mbnl3 muscleblind like splicing factor 3 2 4
MIRT592703 Gfra2 glial cell line derived neurotrophic factor family receptor alpha 2 2 2
MIRT592746 Epas1 endothelial PAS domain protein 1 2 2
MIRT592765 Dmd dystrophin, muscular dystrophy 2 4
MIRT592843 Akap2 A kinase (PRKA) anchor protein 2 2 2
MIRT592989 Cacna2d2 calcium channel, voltage-dependent, alpha 2/delta subunit 2 2 2
MIRT593003 Bend4 BEN domain containing 4 2 2
MIRT593228 Fndc3a fibronectin type III domain containing 3A 2 2
MIRT593415 Neurod2 neurogenic differentiation 2 2 4
MIRT593453 Rab11fip1 RAB11 family interacting protein 1 (class I) 2 2
MIRT593482 Havcr2 hepatitis A virus cellular receptor 2 2 2
MIRT593517 Csf2ra colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) 2 2
MIRT594037 2810006K23Rik RIKEN cDNA 2810006K23 gene 2 2
MIRT594129 Fam104a family with sequence similarity 104, member A 1 1
MIRT595670 Rxrb retinoid X receptor beta 2 2
MIRT595701 Fndc7 fibronectin type III domain containing 7 2 2
MIRT596268 Bnc2 basonuclin 2 2 2
MIRT596281 Slc6a8 solute carrier family 6 (neurotransmitter transporter, creatine), member 8 2 2
MIRT598763 Gpr68 G protein-coupled receptor 68 2 2
MIRT601111 Btrc beta-transducin repeat containing protein 2 2
MIRT601202 Arhgef9 CDC42 guanine nucleotide exchange factor (GEF) 9 2 2
MIRT603151 Ubxn8 UBX domain protein 8 2 2
MIRT603192 Trim65 tripartite motif-containing 65 2 2
MIRT604581 Trim71 tripartite motif-containing 71 2 2
MIRT605447 St18 suppression of tumorigenicity 18 2 2
MIRT605591 Ncam1 neural cell adhesion molecule 1 2 2

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