pre-miRNA Information
pre-miRNA mmu-mir-466e   
Genomic Coordinates chr2: 10479088 - 10479171
Synonyms mmu-mir-466e, Mir466e
Description Mus musculus miR-466e stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-466e-3p
Sequence 51| UAUACAUACACGCACACAUAAGA |73
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Tacr2   
Synonyms Nk2r, Skr, Tac2r
Description tachykinin receptor 2
Transcript NM_009314   
Expression
Putative miRNA Targets on Tacr2
3'UTR of Tacr2
(miRNA target sites are highlighted)
>Tacr2|NM_009314|3'UTR
   1 AGTCTTGAGCTTGTCTGCAGGGCCCAGGCTTAGCCCCTTTGAGAATCGGCTGATGGAAAAGCCCTTACATAATAGCTTGT
  81 CCCACACCCCATTCTTCAACACACCAACAGAAAAGAAGCAAGACGGTGCTCAGAGCGCCACAGGCAATGCTGATACAAAG
 161 AGCTTGGAGAGCAGACACCCATCATAGGCATTCCAGCAACCGAGGAAGGGGCCAGGAAGACTGGGAACCAACATTTGGCC
 241 AATCCAGGAGCTGCAAAGTGAGTTGTCTCCCGGGAGTATAGAGAGGGGTGGAAAAACGATCTCAGGGAAGACAACTGCAG
 321 TCATAATGAGAAAGGCAGCTGAAGGATGGTGACATGGCTCAGCAGCGAGGTCGTAAAGCCACTCTCTGCTAAGCCTCATG
 401 ACCTAAGTGCAGTTCCTGAAACCCACACAGAAGGAAAGAACCAACGTCTGCAAGCTGTCCCCTGACCTCCCCAAGTGTGT
 481 GTATGCACATGTGTGTGTGCATGTATATCTGCACATGTATGCACACACACACACACACACACACATGTACATGTGCCTGT
 561 GCACATACAATGTTAAAGAGAAAGAAGGCAACCACTCCAGTGTTAGGGTCACAGGAAAAGGTGGGGACTGTGGCAGCCTG
 641 CAGAGGACAAGCACCCAAAGGGAAACTCAGCTGCTTTGCTCTGGAACACAGTTGCCAGGTTTTCCAAATAAAGCCTGAGA
 721 TCT
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' agaaUACACACGCACAUACAUAu 5'
              |||||||:||| |||||| 
Target 5' gcacATGTGTGTGTGCATGTATa 3'
485 - 507 159.00 -23.40
2
miRNA  3' agaauacacacgcaCAUACAUAu 5'
                        ||:|||:| 
Target 5' cctgacctccccaaGTGTGTGTa 3'
461 - 483 113.00 -11.12
3
miRNA  3' agaaUACA--CACGCACAUACAUau 5'
              ||||   ||:|  |:|| |  
Target 5' acacATGTACATGTGCCTGTGCAca 3'
541 - 565 89.00 -8.30
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1
miRNA  3' agaaUACACACGCACAUACAUAu 5'
              |||||||:||| |||||| 
Target 5' ---cAUGUGUGUGUGCAUGUAUa 3'
1 - 20
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
CLIP-seq Support 1 for dataset GSM622570
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1A
Location of target site NM_009314 | 3UTR | CAUGUGUGUGUGCAUGUAUAUCUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
CLIP-seq Support 2 for dataset GSM622571
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1B
Location of target site NM_009314 | 3UTR | CAUGUGUGUGUGCAUGUAUAUCUG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
186 mmu-miR-466e-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577218 Zfp963 zinc finger protein 963 2 6
MIRT577289 Zfp446 zinc finger protein 446 2 2
MIRT577554 Tdrd1 tudor domain containing 1 2 6
MIRT577573 Tctn3 tectonic family member 3 2 2
MIRT577705 Slc28a3 solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 2 2
MIRT577720 Slc22a15 solute carrier family 22 (organic anion/cation transporter), member 15 2 2
MIRT577765 Serpinb10 serine (or cysteine) peptidase inhibitor, clade B (ovalbumin), member 10 1 1
MIRT577864 Rassf5 Ras association (RalGDS/AF-6) domain family member 5 2 2
MIRT577925 Prl5a1 prolactin family 5, subfamily a, member 1 2 2
MIRT577975 Pira2 paired-Ig-like receptor A2 2 2
MIRT577982 Pira1 paired-Ig-like receptor A1 2 2
MIRT578457 Ints12 integrator complex subunit 12 2 2
MIRT578576 Hist1h1d histone cluster 1, H1d 2 2
MIRT578612 Haus2 HAUS augmin-like complex, subunit 2 2 2
MIRT578669 Gramd1c GRAM domain containing 1C 2 2
MIRT578694 Golt1a golgi transport 1A 2 2
MIRT578832 Fech ferrochelatase 2 2
MIRT578857 Mtfr2 mitochondrial fission regulator 2 1 3
MIRT578898 Erich1 glutamate rich 1 2 2
MIRT579230 Ccdc138 coiled-coil domain containing 138 2 2
MIRT579236 Casp8 caspase 8 2 2
MIRT579263 Bub1 BUB1, mitotic checkpoint serine/threonine kinase 2 2
MIRT579331 Atf7 activating transcription factor 7 2 2
MIRT579464 Acot11 acyl-CoA thioesterase 11 2 4
MIRT579502 A630001G21Rik RIKEN cDNA A630001G21 gene 2 2
MIRT579550 Tmem241 transmembrane protein 241 1 1
MIRT579628 Mettl20 electron transfer flavoprotein beta subunit lysine methyltransferase 1 1
MIRT579756 Zfp608 zinc finger protein 608 2 2
MIRT579920 Zbtb39 zinc finger and BTB domain containing 39 2 2
MIRT580179 Twist2 twist basic helix-loop-helix transcription factor 2 2 2
MIRT580204 Ttc7 tetratricopeptide repeat domain 7 2 2
MIRT580221 Tspan12 tetraspanin 12 2 2
MIRT580287 Trhr thyrotropin releasing hormone receptor 2 2
MIRT580313 Tnrc6b trinucleotide repeat containing 6b 2 2
MIRT580389 Slc35g1 solute carrier family 35, member G1 1 1
MIRT580402 Tmem167 transmembrane protein 167 2 2
MIRT580422 Tmem161b transmembrane protein 161B 2 2
MIRT580482 Tigd5 tigger transposable element derived 5 2 2
MIRT580511 Tet3 tet methylcytosine dioxygenase 3 2 2
MIRT580643 Stxbp6 syntaxin binding protein 6 (amisyn) 2 4
MIRT580700 Stard8 START domain containing 8 2 2
MIRT580754 Spry1 sprouty homolog 1 (Drosophila) 2 2
MIRT580818 Snx27 sorting nexin family member 27 2 2
MIRT580863 Slc8a1 solute carrier family 8 (sodium/calcium exchanger), member 1 2 2
MIRT580877 Slc7a11 solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 2 2
MIRT581027 Sipa1l1 signal-induced proliferation-associated 1 like 1 2 2
MIRT581107 Sept8 septin 8 2 2
MIRT581119 Sept3 septin 3 2 2
MIRT581208 Satb2 special AT-rich sequence binding protein 2 2 2
MIRT581412 Rbm47 RNA binding motif protein 47 2 4
MIRT581498 Pvrl4 nectin cell adhesion molecule 4 2 2
MIRT581511 Ptpro protein tyrosine phosphatase, receptor type, O 2 2
MIRT581550 Ptdss1 phosphatidylserine synthase 1 2 2
MIRT581612 Prkcd protein kinase C, delta 2 2
MIRT581621 Prkca protein kinase C, alpha 2 2
MIRT581701 Ppm1k protein phosphatase 1K (PP2C domain containing) 2 2
MIRT581727 Pofut1 protein O-fucosyltransferase 1 2 2
MIRT581758 Plxna1 plexin A1 2 2
MIRT581814 Plag1 pleiomorphic adenoma gene 1 2 2
MIRT581840 Piwil2 piwi-like RNA-mediated gene silencing 2 2 2
MIRT581872 Pik3ca phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha 2 2
MIRT581889 Pias1 protein inhibitor of activated STAT 1 2 2
MIRT581924 Pgm2l1 phosphoglucomutase 2-like 1 2 2
MIRT581979 Pcsk5 proprotein convertase subtilisin/kexin type 5 2 2
MIRT582089 Ogdh oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) 2 2
MIRT582162 Nln neurolysin (metallopeptidase M3 family) 2 2
MIRT582194 Nfkbia nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha 2 2
MIRT582310 Nacc2 nucleus accumbens associated 2, BEN and BTB (POZ) domain containing 2 8
MIRT582534 Mapk1 mitogen-activated protein kinase 1 2 2
MIRT582686 Lhfpl4 lipoma HMGIC fusion partner-like protein 4 2 6
MIRT582901 Il3 interleukin 3 2 2
MIRT582926 Il15ra interleukin 15 receptor, alpha chain 2 2
MIRT582947 Ikzf5 IKAROS family zinc finger 5 2 2
MIRT583009 Iars2 isoleucine-tRNA synthetase 2, mitochondrial 2 2
MIRT583029 Htt huntingtin 2 2
MIRT583120 Hecw1 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 2 2
MIRT583215 Gpr123 adhesion G protein-coupled receptor A1 2 6
MIRT583298 Glra2 glycine receptor, alpha 2 subunit 2 2
MIRT583335 Paxbp1 PAX3 and PAX7 binding protein 1 1 3
MIRT583370 Gabra2 gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2 2 2
MIRT583404 Fzd3 frizzled class receptor 3 2 2
MIRT583537 Fbxo45 F-box protein 45 2 2
MIRT583598 Fam63b MINDY lysine 48 deubiquitinase 2 2 2
MIRT583638 Tmem236 transmembrane protein 236 1 1
MIRT583975 Dennd4a DENN/MADD domain containing 4A 2 2
MIRT584001 Dcdc2a doublecortin domain containing 2a 2 2
MIRT584050 Cysltr1 cysteinyl leukotriene receptor 1 2 2
MIRT584093 Csrnp3 cysteine-serine-rich nuclear protein 3 2 2
MIRT584300 Chic1 cysteine-rich hydrophobic domain 1 2 2
MIRT584316 Chek1 checkpoint kinase 1 2 6
MIRT584366 Cdk19 cyclin-dependent kinase 19 2 2
MIRT584724 Atp8b1 ATPase, class I, type 8B, member 1 2 2
MIRT584781 Arl8a ADP-ribosylation factor-like 8A 2 2
MIRT584804 Arl15 ADP-ribosylation factor-like 15 2 2
MIRT584827 Arhgef33 Rho guanine nucleotide exchange factor (GEF) 33 2 2
MIRT584906 Alg6 asparagine-linked glycosylation 6 (alpha-1,3,-glucosyltransferase) 2 2
MIRT584953 Adamts4 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 2 6
MIRT585037 Lrrc71 leucine rich repeat containing 71 1 1
MIRT585057 Pcnxl4 pecanex homolog 4 1 1
MIRT585104 Zfp941 zinc finger protein 941 2 2
MIRT585152 Zfp874b zinc finger protein 874b 2 2
MIRT585337 Ybey ybeY metallopeptidase 2 2
MIRT585398 Wfdc12 WAP four-disulfide core domain 12 2 2
MIRT585482 Txlnb taxilin beta 2 2
MIRT585516 Trp53rk transformation related protein 53 regulating kinase B 2 2
MIRT585552 Tpte transmembrane phosphatase with tensin homology 2 2
MIRT585706 Tbc1d24 TBC1 domain family, member 24 2 2
MIRT585749 Stat1 signal transducer and activator of transcription 1 2 2
MIRT585894 Slc1a7 solute carrier family 1 (glutamate transporter), member 7 2 2
MIRT585919 Slc16a9 solute carrier family 16 (monocarboxylic acid transporters), member 9 2 2
MIRT586027 Sass6 SAS-6 centriolar assembly protein 2 4
MIRT586036 Rsad1 radical S-adenosyl methionine domain containing 1 2 2
MIRT586137 Rbm41 RNA binding motif protein 41 2 2
MIRT586185 Ptprr protein tyrosine phosphatase, receptor type, R 2 2
MIRT586347 Pdxk pyridoxal (pyridoxine, vitamin B6) kinase 2 2
MIRT586392 Padi2 peptidyl arginine deiminase, type II 2 4
MIRT586426 Oip5 Opa interacting protein 5 2 2
MIRT586504 Neu4 sialidase 4 2 2
MIRT586627 Mfsd2b major facilitator superfamily domain containing 2B 2 2
MIRT586676 Mbd4 methyl-CpG binding domain protein 4 2 2
MIRT586952 Gstt3 glutathione S-transferase, theta 3 2 2
MIRT587235 Nxpe3 neurexophilin and PC-esterase domain family, member 3 1 1
MIRT587548 Cxcl5 chemokine (C-X-C motif) ligand 5 2 2
MIRT587740 Cd28 CD28 antigen 2 2
MIRT587785 Ccpg1 cell cycle progression 1 2 2
MIRT587902 BC003965 cDNA sequence BC003965 2 2
MIRT588023 Akap7 A kinase (PRKA) anchor protein 7 2 2
MIRT588064 Fam211b leucine rich repeat containing 75B 1 1
MIRT588077 AI429214 expressed sequence AI429214 2 2
MIRT588154 Ccdc169 coiled-coil domain containing 169 1 1
MIRT588370 Zfp462 zinc finger protein 462 2 2
MIRT588437 Ythdc2 YTH domain containing 2 2 2
MIRT588493 Wbp4 WW domain binding protein 4 2 2
MIRT588518 Uty ubiquitously transcribed tetratricopeptide repeat gene, Y chromosome 2 2
MIRT588605 Tsc22d3 TSC22 domain family, member 3 2 2
MIRT588652 Tmem200a transmembrane protein 200A 2 2
MIRT588747 Synj2bp synaptojanin 2 binding protein 2 2
MIRT588778 Strbp spermatid perinuclear RNA binding protein 2 2
MIRT588794 Ss18l1 SS18, nBAF chromatin remodeling complex subunit like 1 2 6
MIRT588817 Srgap3 SLIT-ROBO Rho GTPase activating protein 3 2 2
MIRT588838 Sort1 sortilin 1 2 2
MIRT588962 Scn2a1 sodium channel, voltage-gated, type II, alpha 2 2
MIRT589067 Rbbp9 retinoblastoma binding protein 9, serine hydrolase 2 2
MIRT589077 Rbbp5 retinoblastoma binding protein 5, histone lysine methyltransferase complex subunit 2 2
MIRT589087 Rasal2 RAS protein activator like 2 2 2
MIRT589114 Rasa2 RAS p21 protein activator 2 2 2
MIRT589121 Rai1 retinoic acid induced 1 2 2
MIRT589139 Rad51d RAD51 paralog D 1 1
MIRT589277 Pitpnb phosphatidylinositol transfer protein, beta 2 2
MIRT589409 Nrf1 nuclear respiratory factor 1 2 2
MIRT589459 Nceh1 neutral cholesterol ester hydrolase 1 2 2
MIRT589513 Mtf1 metal response element binding transcription factor 1 2 2
MIRT589634 Loxl3 lysyl oxidase-like 3 2 2
MIRT589758 Iws1 IWS1, SUPT6 interacting protein 2 2
MIRT589896 Gtpbp2 GTP binding protein 2 2 2
MIRT589946 Gfod1 glucose-fructose oxidoreductase domain containing 1 2 2
MIRT590058 Fbxl17 F-box and leucine-rich repeat protein 17 2 4
MIRT590129 Ephb3 Eph receptor B3 2 2
MIRT590405 Ccna2 cyclin A2 2 2
MIRT590436 Casz1 castor zinc finger 1 2 2
MIRT590466 Car5b carbonic anhydrase 5b, mitochondrial 2 2
MIRT590476 Camsap2 calmodulin regulated spectrin-associated protein family, member 2 1 1
MIRT590512 Bsn bassoon 2 6
MIRT590570 B3gnt2 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 2 2
MIRT590731 Ado 2-aminoethanethiol (cysteamine) dioxygenase 2 2
MIRT590816 1110059G10Rik RIKEN cDNA 1110059G10 gene 2 2
MIRT591122 Oas3 2'-5' oligoadenylate synthetase 3 2 2
MIRT591368 E2f1 E2F transcription factor 1 2 2
MIRT592056 Tbc1d13 TBC1 domain family, member 13 2 2
MIRT592232 Ly96 lymphocyte antigen 96 2 2
MIRT592722 Fbxl14 F-box and leucine-rich repeat protein 14 2 6
MIRT592778 Diap2 diaphanous related formin 2 2 6
MIRT593254 Tas1r3 taste receptor, type 1, member 3 2 2
MIRT594456 Evi2b ecotropic viral integration site 2b 2 2
MIRT594616 Myt1l myelin transcription factor 1-like 2 2
MIRT594672 Ehf ets homologous factor 2 2
MIRT594751 Wdr89 WD repeat domain 89 2 2
MIRT595407 Arid2 AT rich interactive domain 2 (ARID, RFX-like) 2 2
MIRT595645 Abi3bp ABI gene family, member 3 (NESH) binding protein 2 2
MIRT603345 Slc16a10 solute carrier family 16 (monocarboxylic acid transporters), member 10 2 2
MIRT604327 Bpifc BPI fold containing family C 2 2
MIRT604500 2510049J12Rik makorin, ring finger protein 2, opposite strand 2 2
MIRT604864 Mef2a myocyte enhancer factor 2A 2 2
MIRT605407 Tacr2 tachykinin receptor 2 2 2
MIRT605870 Atpbd4 diphthamine biosynthesis 6 2 2
MIRT606108 Unc5c unc-5 netrin receptor C 2 2

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