pre-miRNA Information
pre-miRNA mmu-mir-374b   
Genomic Coordinates chrX: 103573060 - 103573154
Description Mus musculus miR-374b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-374b-5p
Sequence 22| AUAUAAUACAACCUGCUAAGUG |43
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol Gmeb1   
Synonyms 1110050A04Rik, AI256615, AI481278
Description glucocorticoid modulatory element binding protein 1
Transcript NM_001122992   
Other Transcripts NM_020273   
Expression
Putative miRNA Targets on Gmeb1
3'UTR of Gmeb1
(miRNA target sites are highlighted)
>Gmeb1|NM_001122992|3'UTR
   1 TTGGGGACAGGGCCAAGAGCTGTGTTTTGGTTTGGAATTTTAATTATGTTTATTTTTATCATTGTCCCGCTCATTTCCAC
  81 ATAGAACTTTTTTTAAAAAAAAAACAAAAACAAACAAAAAAAAACCAAACTACAAAATCTCGTTGTAACTGCAAATGTTG
 161 GGTTCCTCCTACTTCCTCATTGACAACTGGACAGAACAAGCTGCCTTTCCAGAAGTTAGAAACAGGGCACCCAGGGTCTG
 241 AACCATCTTTCCCCAAGGAAGGTAATTGCTTTTAGGGGAAGTGTCAAAATATCCAGGAATCCTTTCCAGAATAGTCTGTC
 321 TGTGTATACACGCATGGTCTCACTCTTGTAAAGACGCATTGACCAGACCCTGGAACACAGATCTGACTCCAGAAGATGAC
 401 CAGCTCTGCTGTGGATACAGAAGCACGCTTGCCTCTGTGTCCTGGATATGATGGCCTCAGATGCTGGTGTGCAAAACTGA
 481 AAGAACAGGAGGTTGGCTCTGCTTAGGGTGGAAAGTAGTGTGATCAGCAGCTCATAGGGGGAGCCCTTTGACAGGGAGGG
 561 GCCGAGGAATGGTCTTCCAGAGGTGCATCTGATGCCCCTTGCGCTCTCTGAGGGTTGGGTACCATTTTCAATTTCAGAAG
 641 AGGACAGAGATTATAGAAAGACACAAAGCAATCAAAAGATTGTCACAGCTGTGCTTTTGGGATAGCTCACCTTGCCCCCA
 721 GCCTTGTCAAAGCCCTCACCCTCCTCGCCCAGGACTATCAGTCCATTCCTTTGCAGACTGTGATGTGGTATAAATTGTTC
 801 TAATTCTTGGACTGTGGCTGTCATGGAGGTGGGGCTCCAAGGCCATCATTTTTCGTTTCTTGACTCCAGGAATGGCAGAG
 881 TCCCTCGTGCAGACCAGCTGCTATTTTTGGTGGAGCCCTGCACGTAAGCATGCAAGTGTGTGTGTGTGTGTGTGTATGTG
 961 TGTGTGTGTGTGTGTGTGTGTGTGTGTGTACATGTTTCGGGTTTGGAACCAAAAGCAGTCATTTCCCTGGTGCTTCCTTT
1041 TCTTTTCCTTCCGTCTGCTGGACCCCATGCTCAGTAGCTTTTTGGCAGACAGCAGTGACAGGTGCCTGCCTGCCTTCATT
1121 CTCTTTGCCATTCTCCTCTTCTGATACCACAGATGTTTCTGTGAGATACCGAACTCATAAAATGGCTTAGGCTTGGCAGG
1201 GACAACTGGTAACACCTTTCCTGAGGTGTGGACTAGATGGCTCCTGAGTGAGCTGCACTTTCTGGGAAAGGGACATTGTA
1281 TTTGACATTGTAAGAAGTATGACAGGAGTGTCAGTAACGTCCAGGCGTGGGGCTTCTTCCTAAATGCTGTTTGTACATTC
1361 GGTACTACTAGGTCACTCTTTCTGCTGTGATCCCATGTCGTTGATTCTCTTCAGCGTTTGGGGGAGAGTAAGCAGTTTCT
1441 GGTTTCTTTAATTATATTGTTTACTATGTTAGGGAAAATATAGAGACTCTCGAGGTGACTTTATAGGTCAAAAAAGCATA
1521 CAGCTTAGGAGCTGGAGGATTTATTATTATTATTAAAAGTAAAATTTTGGCTTTTGTTTAAAAAAAAAAAATAGAGAAGG
1601 ATGCCTGTCTAAATCTGTCTTCCTGCCTGCAGGTTTTGAAAACCCTGTAAGCAACCAAAAGTTGCACCTTCCATCTCTTA
1681 GACGGCCATGTGTCACCAGGGAAGCAAGCCCTCAGGGATCACTCTGAAGACCCTGAAGAGTGCGGCTTCTGAACTCTATT
1761 CAGATTTGTTTCAGCTTTAAAACTGGACTTTTCTTTTCAGACTCTTTAGTTAGGCTGGGGACAGAACTGAATTGGTAAAG
1841 TGGTCACCTAGCGTTCATGAAACCCTGGGCTCAGTCTCCAGCCAGAAGTAAACTGGGCATGGGGCCACATATCTGATCCT
1921 AACACTTGGGAAATAGAGACGGGAGACTTAGAAATTCAAGGTCATACATACCAAGTTCAAGACCAACCTGGGATATATGA
2001 AATAGTGTCTCAAAAAATTTTTGTTTAGGACAAGCTTTTGGTGAAAGAAGGAATTTATTCCTGCCTTCCTTACACTTTGT
2081 TAATACATAGGAATTTTTATTACATTTATTTATCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGATGGTG
2161 AGCTTGCTACGGTATGTATGTGAAGATCAGAGAACAACCTGCAGGAGTCTGTTCTCGCCTTCCACCATGTGGGTCCTGGG
2241 AATTGAACTCAGGTTGCCAGGCTTGGTGGTAAGCACCCTCATCCATTGAGCCATCTTGCCTGCTCTAAAAGTTTCGGTCT
2321 TCAGACCAAAACTGTTACTTGAACCTAGAAAGCCACATCGCATCCCTGTTGTGTCCTTTAGGTCATTTCTCTTTTCCAGA
2401 GATGGGCAGACCAATTCAGGACACGGGAGGCACCCTAGAGAATAGAGGCAGGCTCCTTCAGGTCCTGCCTTTTTGCTCTT
2481 CACAGCTGTTGTGTCATTAAAGGTTGTGTGAGCAGCATGCTTCCAAAGTCAGATTTCCTTCTTGAGACAGAGCCTGTTTA
2561 CTTAAAACAGATTACATGAGCTTTGCTGTTTTCCTGGTCTTCCAATTGCTGTCATTGCTAGCTGCAGTGGTACTTGGAGA
2641 GATGAGATCTCTCACAGACCTGGTCTAGATTATTTTAGGTGGGGTGATACACATGCAAGCTTCTGATGTGTGAATAGGCA
2721 GTTGCAATGAAATTACTCCTCAGGCTGACTTGATGGTAGCTATCTTTTCTCTAGCCATTCATTAAATAAACATTTGTAAG
2801 TACTGTCTATGTATAAGTACTAAACGCCAACTGATTGCGTCACCTAAGCCCCAGTAAGTACCATGAAGATGCAGGTTTTG
2881 TGCTAGACACTGATCTTGCAGACAAGACAATTCCTGTTCTTAAGAAGTTCATACCCAGACATCTTTGCAGTTAACCACAC
2961 TTTTTGTTTGTTTGGTTTGTTTTTTGAGACAGGGTTTCTCTGTGTAGCCCTGGCTGTCCTGGAACTCTCACTATGTAGCC
3041 AGGCTGACTGGAACTCACAGAGATCTGTTTGCCTCTGTCTTCCAAGTGCTGGGATTAAAGGTGTGCACCACCACTGCCAT
3121 AGTGAATAGTCAACCGTATTTTAATGTCATTGGTTTCTTTTGTGATCCTGTTTTATCTTAAATATTGGGAAGGACTCTGA
3201 GTCTTTAGTTCCTAGGTTTCAACAGGCAAAGTCACAGAGGTGAAGCCACCCCTGGTGAGATTTTACCTTAGGTTGAGGTG
3281 ACTGTAGCTATGGTCTTTTTTTTTTTTTTTAAAGATTTATTTATTTATTATATGTAAGTACACTGTAGCTGTCTTCAGAC
3361 ACTCCAGAAGAGGGAGTCAGATCTTGTTACAGATGGCTGTGAGCCACCATGTGGTTGCTGGGATTTGAACTCTGGACCTT
3441 CGGAAGAACAGTCGGGTGCTCTTACCCACTGAGCCATCTCACCAGCCCCAGCTATGGTCTTTTGGCCTGGAGTTAAGCAC
3521 AAAAGATCAAGCACTTACTCAGCTTGGGGTCTGAATGGCTTCCGCTAATCTGAGGCAGTTCTCTGCATACCCTAGTTGCC
3601 CTTGAACTTGGCTATGTAGAGTAGCCTGGCTTTGAGCTCATAAAGGTCCATCTGCCTCTGCCTCCTAAGCATTGGGATTA
3681 AAGCTATCACACTTGGCTCTTGCTACTCCTCAGTTTCTAGGCTTCCAAAGAGTGGTGCTGCATGCGTGCCTGGGGCCCCG
3761 GCCCCAGAGGGCAGGGGCAAGAAGAGAGAAAGCTTCAAGATCCTGTCTCACAAACCAGAAATTTAGGCTTCCACATCCCA
3841 AACCCAAAGTTACTCTACTTTTGTTTGTGTCGCTCCCCCCTCCCCCATGGACAATAGACATTCAAGATTCAAACCCACTT
3921 TGGTGTGTCGTTCCCTCACCTGCCATAGCAGGAGAGAGAACTAATATAGATGAATAGCAGGTGATTCTTTTAATGTTGCT
4001 GGTTTGTCCCATTTCATGAGAGACACTATAGATAAGTATATATGGGGAGTACTGATTTTATTTACAAAAAAAAAAAATGT
4081 ACATTTAGTGTGCTTGTGTGTGTGTGGCATGCCGGAGTACATGGTGTCTTTATCAATGAACAGCTTTCAGGATTCAGTTC
4161 TCGCCTTCCACTGTGTGGGTCCCAGGGGTCAAATTCAGTTTGCCAGGCTTGGCAAGCAAGCACCTCTACTCACTGAGCTA
4241 TCTTGCTGGCCCAATGAGATTTTTTCTTTTTCATGTATTTATTTTAGTTTATAATTGTTCTTGCAAGTATGTATGTGCAC
4321 CTCACCTCTCACGCATGCCTGGTATCTGACGTGGTTGGTCGGAGAACATTTCTGATCCCCTGGAACTGGTGTTAGAGATG
4401 GTGTCTGCTACCATGTGGGCGCTGGAAATCCAACCCAGGTTCTCTGAATGAATACATGCTCTCAACTGAGCTGTCTCTTC
4481 GGGTGATATTATAAATAACAAAACTGTTGTGAAATATGGAGTCATTGAGTCAGAACTGACCCATAATGGAGGACTATCTA
4561 GGAGATGGAAAAGAATCCTTCCCTCAACCCTCCCCAAAAAAGGTTGAATTCTGAAATTAAGCACAAAGTGGTGGTGGCAC
4641 ACATCTTTGATCCCAGCACGTAGGAGCCAGTTGGATCTCTGAGTTCAAGGCCAGACTTGTCTCGAGGACAGCCAAGGCTA
4721 CACAGAGAAATCACATCTTG
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' guGAAUCGUCCAACAUAAUAUa 5'
            |||  |:|||  ||||||| 
Target 5' ctCTT--CGGGTGATATTATAa 3'
4475 - 4494 160.00 -10.30
2
miRNA  3' guGAA---UCG---UCCAACAUAAUAua 5'
            |||   |||   ||| | ||||||  
Target 5' agCTTAGGAGCTGGAGGATTTATTATta 3'
1522 - 1549 140.00 -6.60
3
miRNA  3' gugAAUCGUCCA--AC--AUA-AUAUa 5'
             || |:| ||  ||  ||| |||| 
Target 5' acaTTTGTAAGTACTGTCTATGTATAa 3'
2790 - 2816 123.00 -5.80
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622571. RNA binding protein: AGO2. Condition:WT1B ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
76 mmu-miR-374b-5p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT418787 Map2 microtubule-associated protein 2 1 1
MIRT577252 Zfp84 zinc finger protein 84 1 1
MIRT577346 Zfp11 zinc finger protein 11 1 1
MIRT577582 Tcf21 transcription factor 21 1 1
MIRT577695 Slc35d2 solute carrier family 35, member D2 1 1
MIRT577869 Rassf5 Ras association (RalGDS/AF-6) domain family member 5 1 1
MIRT577940 Ppp6r1 protein phosphatase 6, regulatory subunit 1 1 1
MIRT577949 Pou4f2 POU domain, class 4, transcription factor 2 1 1
MIRT578202 Nadsyn1 NAD synthetase 1 1 1
MIRT578468 Impg2 interphotoreceptor matrix proteoglycan 2 1 1
MIRT578587 Hist1h1d histone cluster 1, H1d 1 1
MIRT579056 Cradd CASP2 and RIPK1 domain containing adaptor with death domain 1 1
MIRT579823 Zfp113 zinc finger protein 113 1 2
MIRT579840 Zer1 zyg-11 related, cell cycle regulator 1 1
MIRT579950 Xpo7 exportin 7 1 1
MIRT579992 Wnt3 wingless-type MMTV integration site family, member 3 1 1
MIRT579997 Wnt11 wingless-type MMTV integration site family, member 11 1 1
MIRT580191 Ttll7 tubulin tyrosine ligase-like family, member 7 1 1
MIRT580256 Trim2 tripartite motif-containing 2 1 1
MIRT580383 Tmem206 transmembrane protein 206 1 2
MIRT580415 Tmem161b transmembrane protein 161B 1 1
MIRT580534 Tead3 TEA domain family member 3 1 1
MIRT580565 Tbx4 T-box 4 1 1
MIRT580611 Syp synaptophysin 1 1
MIRT581171 Sec23ip Sec23 interacting protein 1 1
MIRT581538 Pten phosphatase and tensin homolog 1 1
MIRT581571 Prrx1 paired related homeobox 1 1 1
MIRT581741 Plxnc1 plexin C1 1 1
MIRT581794 Plaur plasminogen activator, urokinase receptor 1 1
MIRT581822 Pkib protein kinase inhibitor beta, cAMP dependent, testis specific 1 1
MIRT581971 Pdcd10 programmed cell death 10 1 1
MIRT582055 Osbp oxysterol binding protein 1 1
MIRT582142 Npr3 natriuretic peptide receptor 3 1 1
MIRT582168 Nkx2-2 NK2 homeobox 2 1 1
MIRT582469 Mex3a mex3 RNA binding family member A 1 1
MIRT582518 March5 membrane-associated ring finger (C3HC4) 5 1 2
MIRT582589 Lrrtm2 leucine rich repeat transmembrane neuronal 2 1 1
MIRT582667 Lmtk2 lemur tyrosine kinase 2 1 1
MIRT583063 Hoxa11 homeobox A11 1 1
MIRT583111 Hexim1 hexamethylene bis-acetamide inducible 1 1 1
MIRT583233 Gpc6 glypican 6 1 1
MIRT583395 Fzd5 frizzled class receptor 5 1 1
MIRT583429 Frmpd4 FERM and PDZ domain containing 4 1 1
MIRT583613 Fam46a family with sequence similarity 46, member A 1 1
MIRT583848 Eea1 early endosome antigen 1 1 1
MIRT584669 B3gat1 beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) 1 1
MIRT584731 Atp2b2 ATPase, Ca++ transporting, plasma membrane 2 1 1
MIRT584872 Ap3m1 adaptor-related protein complex 3, mu 1 subunit 1 1
MIRT585039 Lrrc71 leucine rich repeat containing 71 1 1
MIRT585303 Zfp248 zinc finger protein 248 1 3
MIRT585725 T2 brachyury 2 1 1
MIRT585759 St8sia3 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3 1 1
MIRT585986 Sfxn4 sideroflexin 4 1 1
MIRT586181 Ptprr protein tyrosine phosphatase, receptor type, R 1 1
MIRT586738 Lcn5 lipocalin 5 1 1
MIRT586778 Jmjd4 jumonji domain containing 4 1 1
MIRT587048 Gm5531 transmembrane epididymal protein 1B 1 1
MIRT587444 Dimt1 DIM1 dimethyladenosine transferase 1-like (S. cerevisiae) 1 1
MIRT588190 A130010J15Rik RIKEN cDNA A130010J15 gene 1 1
MIRT588690 Tet2 tet methylcytosine dioxygenase 2 1 1
MIRT589450 Nck2 non-catalytic region of tyrosine kinase adaptor protein 2 1 1
MIRT590325 Csgalnact2 chondroitin sulfate N-acetylgalactosaminyltransferase 2 1 1
MIRT590458 Car5b carbonic anhydrase 5b, mitochondrial 1 1
MIRT590752 Actr10 ARP10 actin-related protein 10 1 1
MIRT594398 Ints6 integrator complex subunit 6 1 1
MIRT594639 Mex3c mex3 RNA binding family member C 2 1
MIRT594870 Man1a2 mannosidase, alpha, class 1A, member 2 1 1
MIRT595871 Csmd1 CUB and Sushi multiple domains 1 1 1
MIRT596012 Ceacam1 carcinoembryonic antigen-related cell adhesion molecule 1 1 1
MIRT596332 Rpp14 ribonuclease P 14 subunit 1 1
MIRT600146 Zfp1 zinc finger protein 1 1 1
MIRT601141 Bcl11b B cell leukemia/lymphoma 11B 1 1
MIRT601571 Sumf2 sulfatase modifying factor 2 1 1
MIRT605724 Gmeb1 glucocorticoid modulatory element binding protein 1 1 1
MIRT731705 Myf6 myogenic factor 6 3 1
MIRT735635 Socs1 suppressor of cytokine signaling 1 3 0
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-374b Docosahexaenoic acid NULL 445580 Quantitative real-time PCR Caco-2 cells 24623846 2014 up-regulated
miR-374b Glucose NULL 5793 Microarray proximal tubule cell line HK-2 20067797 2010 down-regulated
miR-374b 5-Fluorouracil approved 3385 Microarray MCF-7 breast cancer cells 21506117 2011 down-regulated
miR-374b 5-Fluorouracil approved 3385 Quantitative real-time PCR MCF-7 breast cancer cells 21506117 2011 down-regulated
miR-374b Vitamin D3 approved 5280795 Quantitative real-time PCR Plasma 22594500 2012 up-regulated
miR-374b Marine fungal metabolite 1386A NULL NULL Microarray MCF-7 breast cancer cells. 22159329 2012 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
mmu-miR-374b-5p Valproic Acid 3121 NSC93819 approved sensitive High Seizure tissue
mmu-miR-374b-5p Levetiracetam 5284583 NSC760119 approved sensitive High Seizure tissue
mmu-miR-374b-5p Sunitinib 5329102 NSC750690 approved resistant High Renal Cell Cancer tissue

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