miRTarBase - #MIRT606490


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pre-miRNA Information
pre-miRNA mmu-mir-433   
Genomic Coordinates chr12: 109591715 - 109591838
Synonyms Mirn433, mmu-mir-433, Mir433
Description Mus musculus miR-433 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-433-3p
Sequence 67| AUCAUGAUGGGCUCCUCGGUGU |88
Evidence Experimental
Experiments PCR
Putative Targets

Gene Information
Gene Symbol Cln8   
Synonyms mnd
Description ceroid-lipofuscinosis, neuronal 8
Transcript NM_012000   
Expression
Putative miRNA Targets on Cln8
3'UTR of Cln8
(miRNA target sites are highlighted)		 
>Cln8|NM_012000|3'UTR
   1 CGGCGCCCACCCAGGGACTGGGTGGACCATGATGCCGACTGGGGAGTGTCCAGGGTTGGACAAAGCTCCACAGTGACTCT
  81 TTACGTATGGATGGGGACATAGATAAGTTTATATCTAGGTGAATACTAACTTGTTCCTTACTATGAATTCTAAAGTACCC
 161 ATGAAGTATTGCTGGGGCCTCGACGTGCCTGGAAGAGGTCTTTGCCATCATGACTTTCCTCTGAGTCTTCTGGCGTGGCC
 241 CCAACCACAGCACATCTGCCAAGGCAGTGCTAGGGAAGGAGCAATGGCAGCCAGGTGTGCAGGCTGCTGAGGAAGCCAGT
 321 CTGGAGCCCTGCGACGATTCATCGGGAAGGACCTCGTGTGGGTGTGGGTGTGCCTTATTTGGAAGTCGTGTTCTTTTTCA
 401 TTGCAACAAATTGACCCTAAGAAAATGTGATTTGCTGATCATATTTATTTACTAGCCTGGGGAATTTTAAGCCCGTGGCA
 481 GTGCACTGCCCTCTAGTGGCAAGAGGCAGAAGGACAGAGTTATGGTAAGGGGTTCTCCTTGGCTTACTAGAGTCTGCCCA
 561 CCCCTCCTGGAGTGGTGGTGGTGGTGTGAAATCTTGTACTTCAAAAACAAACAAGCAAACAACAAAAAACCCCAAGGAAC
 641 CTAATAACCTTTTTGAAAAACAAAATAATGAGAATTTTTAGTGATATATTTCTTATTTGACAGAATAAGGTGTCTGGAGG
 721 GGGTCCTGTACTCATCCTCGTGTCGCTCATCCTGAGTCGCTAGGGCCCCGAGCAAGCACCTTGCCTGACATGACCATGGG
 801 AACATGTTCTCGCCGCGCTGTTTGCAGGTTAGACCTCCAGACACCGGCTAGCAATGCTATCGGCAGACACAAAAACTTTC
 881 CCAGAGAACAGTTAAAGAACAAGGGGGTTTTTGTTTGTTTGTTTGTTTGTTTGTTTGGGTTTTTGTTGATTTGTTTTGTC
 961 AAAACTACCTGAGAAATGGTGGTTGCTCTTGAAAATCATGTGTGACTTAGGCTTGCCTCAGCAGCCCTTTAACTTTATGA
1041 GGATGTATGATGCCTATTTTATTATAAAGTAGCCCATTAACTTTATGAGGAGGTATGATGCCTATTTATTTCAATCTAAG
1121 AAAGCCCTTACTTTGTGTTCCTAAGATAATCAACTGAACTGAAAACCCTGTAGCTCCTGTGTCTGCTTTGTTGAGGCACC
1201 AGCCTGGCATTTTTGTTTTTTTTTTGTTTTTTTTTAAGCTGGGCAGAGGCTTAATATAGTGACAGCTCTTTCCAAAACCC
1281 AAACATACTTCCTGTCTGATAACGTGACCCGAAGCTCACCTGAGTGCCTGAGAACACTCTGCGTCCTGAACATGTTGGCA
1361 AGTCTTCAATGGGACCAGGATGGGGTTAGAGAGTGTCTTAGTCGGGCATTTCTATTCCTGCCCAAAACATCATGACCAAG
1441 AAGCAAGTTGGGGAGGAAAGGGTTTATTCAGCTTACACTTTTCCACATGGCTGTTCATCACTAAAGGAAGTCAGGACTAG
1521 AACTCAAGCAGGTCAGGAAGCAGGAGCTGATGCAGAGGCCATGGGTTGATGTGACTTACTGGCTTGCCCTGGCTTTCTCA
1601 GCCTGCTCTCTTAAAGAAGCCAAGACTACCAGCCCAAGGTTGGCACCACCCACAATGGGCCCTCCCCCCTTGATCACTGA
1681 TTGAGAAAATGCCCCACAGCTGGATCTCATGGAGGCGTTTCCTCAAGGGAGGCTCCTTTCTCTGTGGTAACAACAGCTTG
1761 TGTCAAGCTAACACACAGAAGCGGCTGGGACAGGGAGATCATCTGACTTTCGCTGGCCTCTACAAGGAGTGTGACCTCAG
1841 GTTACATCCTCCGTCTTCACGTGTGTCAGCTGAGTGCCATTCCATAGCTCAGGTGACCTTTGGTCACTCCACACACATCT
1921 CCTTTCTGACCAGGACTTCTCCCCGGAACCTTGGAGACAAGTATTCATGAGAAGGCTCTGCAATGGAGCAAGCACTAGTC
2001 CCTGCCTAGAGTAGTGGCTCTCTCGCCATGATTTTTTTAATACAGTTGCTCATGCTGTGGTGATCCCCAACCATGAAGTT
2081 ATTTTCGTTGCTACTTCATAACCGTAATTTTGCTATTATCATAATTAAATATCTGCGTGTCCCAACCTCTTGTGAAAGGG
2161 TCGATCGACCACCAAAGAGGTCATGACCCACAGGTTGAGAACACTGGCCTAGAGAGGGGCCACCCTTCCCAGCTATAGTA
2241 CCTTTGTGGTCTCCAGTGTCCTGCCAGACAGATCTCGGAGAGGGGCATGGTCCAGAGGACACGAGGAGCCGCAGCTCACT
2321 AATGTGAGCCTAGGCTGGCCTGCTCTGTGGCCCACACTGGCCAAGATTCCACTGCACGTGTGCCCCACTAGACTCCTACA
2401 TCAGCTGTTAGGAATTAGAATGGGGTATCGCTTGGTAGTAGAGAACTTACTTAGCATGTAGGAAGCCTTCCTGGCTTCTG
2481 TCTTCAGCCACTGGAGAAGAGACGCTCTCCTACTTGACCGGTATGGAAAATCAAAAACGTTCTCCCTTTCTCGCCACGTA
2561 GATGTTTTACATGTTCCCATGTTTTCAACAGTAGAGGATGTGATGGTCAGGGGCAGCAGCCCAGCGTCTTCCCCATCTCT
2641 TCCCGATGGCTTGCCCTTTTCCCGATGGCTTGCCCTTGCCCCTTCAATTTAGCAACGAGGTTCCAACTTCTCTCAGCAGC
2721 ATACCATAGGCTCCTCAAAGTAGCACTGGACTTGGTGCAGCAGAGCTAGTGACTGGCTGTGTGCTGTTGTCTTCTGTGAA
2801 GCCTATGGGTCACAAGGCTCTCCTTCCACAGACAGCTCCTCAGTAGGCCCTGTGGCTTCCACGGCAACATTGCTAGCAAG
2881 AGGCCATACCCCTACCTGAGCCCTTAAGTCTCTGTTCACACAGCCTGTGGTGACCCTCCTGATGGGAACTAAGCATTGCC
2961 TCATTCCTGTGTGCCGTAGGGGCGACACCTGAGCCTGGGTCCTAGAGAAAGAGCAGAGACTAGCCATTTCTAGTTTTGTA
3041 GGGTGGAGGTCCAAAGTCAGGTGCTGGCAGCTTCAGTCAGGAAGAGGCCCAGTACTTCCTACGTGGAATGTTTCTTTACC
3121 TCCAGGAGAAAGCTATGCCATATATCTAAGGAGCAGAAGGGCTGAAAAGATTCAGGAAGCCCAAGTGAGTTTCCTGGAGG
3201 CCTTTATAAGGATCACTGTCTGGACACATGGTGACTTAAGTGTTTTCTTTGGCTGGGGCTGTCTCTGATTGTGGAAGCTA
3281 GTGGCTGGGAGGAGGCCCTTGCCTATCCCCAACCATGGATGTAATGCGTCCACATAACCTTAGTGGGGCACATTCAAATG
3361 CTAGTACAAAAAAGAGTGTTGCCACACCCCGTGTCAGTAAGATCTCATTCGTTCCACAAAGGGCTGATGCCTCTTGTTCC
3441 CTGTAGAGAGAATATTGTATGTATTGTATGGATGCGTCACCTGTCAATTAAAAAGCCTATGTGTGGCCTACGGCTTAGGC
3521 AAAAACTAGAAGGTGGAACATCCAGGGGGCAGAAAGAATTCTGGGATAGAGCCAGGCAGGAGGTCGCCTGGTAAGACATG
3601 AGGAGACTGATGTGTGGTACCCAAACACAGGTAACCAGCCACATGGCAAAATGTAGATTAAAATAAATTTTAAGTTATGA
3681 TTCTAGTCAGAGAAGAGCCTAGCTATCTGGCCAAGGTATTTTGTAAATATATTTTGAGCCTGAGTCTTATTTCTGAGAGC
3761 ATGGGGCTGGGGGATGGGAGGGAGAACCGGAGGTGATTTTTACAGTTCTCTGACCATTTCCAGTGATGGCTGCCCAGCCA
3841 CTGGCAAGTGAGTGGGTGAACCACCCAATGGAAGGTAACCTCTGTCTTCCAAGTGGGGTCCTGGCAGAAGACATGCTAAC
3921 CCAGAGCTAGACAGTGTTTGCTGAGGGCATATTCAACAAGGTAAGCCAGGGTAGGAGGCCAGGAAGCCAGCTCCATCTCA
4001 CACAGCCAGAAGGGCTTTGGGGAGAGACAGGTAGCCAGTACAGGAACTATGGCGCTAGTCTTTCCTCTCACGGTGACAGA
4081 CCAGTGAGTTGGTTACACAGATTGATACATAGGAGACATTTCTTTTGTTAAAACTATGAAGTGTGTTACAAACTAAAAGG
4161 CTCTGGGTTGTTGGTACAGTGTGAATGTTGGCAGGGCATAGCCTAAAATGGAATTTGCCCCTTGGGATGTTGAGTCTAAC
4241 TCAGGCTGAGGACCAGGCTCAGGCCACGCCTAAGCTTCCCTTCTCTTCAATGCTGGCTGTTTTCGGCCCCCAGTTCCATC
4321 CGAGGGCCTGGAGCTGCAGGAAGAAGCCAGAGCCATCTATGATCTCAGTTCAACAGGCCCCATAAGACTCACCTAGCAGA
4401 AAACCAACAAAAGACACACGTGTCATTAATACTTGATACATTTAAGTCAATTCTGTGTCTAAATTTTTGCTGTGTCCTAA
4481 TGTTTCAGGATATTAATTCAATGTGCCTTTTTTTGACCCCCCCTCCATTTCAGTATACTATGCACATTTTTATTTCTGTT
4561 ACAAATTTTTAATTGCCTAGGGTTAGAGAGAGAGCTCAGTGGTAAATGTTTGTATTGTATACACTAGTCCTCAGGCTCCA
4641 GCCACAGAACCACAAATAAAAAATAAGTTAAAAAAAAAAAAGAACCCCAGCGGCCGCTACTGGATGACCCTGGGCAATGT
4721 GGTGCCCAAGGGGGGATTTCCTCTTCTCTAAAGACAAGGGGGGATGGTAATGGATAGAAGGAATTGTAAGGGTGAGGTTG
4801 GGAGGAGGGACGGCTAGGGCCTGCCATCTTCATGTAAAGTAAATAAACGTTGGTAAAACCCCCAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ugUGG--CUCCUC--G----GGUAGUACUa 5'
            :||  || |||  |    ||||||||| 
Target 5' gtGCCTGGAAGAGGTCTTTGCCATCATGAc 3'
 185 - 214 154.00 -16.50
2 
miRNA  3' uguggcuccucggGUAGUACUa 5'
                       |||||||| 
Target 5' ttcctgcccaaaaCATCATGAc 3'
 1415 - 1436 145.00 -11.20
3 
miRNA  3' ugUGGCUCCUCGGGUAGUACUa 5'
            ||| | |||   :|||||| 
Target 5' ccACCAAAGAG---GTCATGAc 3'
 2169 - 2187 141.00 -12.00
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' uguggCUCCU----CGGGU-AGUacua 5'
               |||||    |:::| |||    
Target 5' uugggGAGGAAAGGGUUUAUUCAgcuu 3'
 		15 - 41
Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
15 mmu-miR-433-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT005344 Rtl1 retrotransposon-like 1 1 1
MIRT577522 Tirap toll-interleukin 1 receptor (TIR) domain-containing adaptor protein 1 1
MIRT579573 4933427D06Rik RIKEN cDNA 4933427D06 gene 1 1
MIRT580107 Ubr1 ubiquitin protein ligase E3 component n-recognin 1 1 1
MIRT587336 Egfr epidermal growth factor receptor 1 1
MIRT588205 Tmem239 transmembrane 239 1 4
MIRT592068 Supt7l suppressor of Ty 7-like 1 1
MIRT592237 LOC100048884 major urinary protein 18 1 1
MIRT594062 Wdr26 WD repeat domain 26 1 1
MIRT594086 Scoc short coiled-coil protein 1 1
MIRT594798 Sgk3 serum/glucocorticoid regulated kinase 3 1 1
MIRT596398 Slc16a7 solute carrier family 16 (monocarboxylic acid transporters), member 7 1 1
MIRT601400 Utp23 UTP23 small subunit processome component 1 1
MIRT605117 Colec12 collectin sub-family member 12 1 1
MIRT606490 Cln8 ceroid-lipofuscinosis, neuronal 8 1 1
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-433 ACTH approved 16132265 Microarray adrenal gland 22128032 2012 down-regulated
miR-433 Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) NULL 8490 Microarray mouse brain 19270793 2009 down-regulated
miR-433 Glucose NULL 5793 Microarray endothelial cells 24394957 2014 up-regulated

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