pre-miRNA Information
pre-miRNA mmu-mir-743b   
Genomic Coordinates chrX: 66777256 - 66777332
Description Mus musculus miR-743b stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA mmu-miR-743b-3p
Sequence 46| GAAAGACAUCAUGCUGAAUAGA |67
Evidence Experimental
Experiments Cloned
Putative Targets

Gene Information
Gene Symbol 0610030E20Rik   
Synonyms 1500031N04Rik, 2810411C16Rik, AI461894
Description RIKEN cDNA 0610030E20 gene
Transcript NM_026696   
Expression
Putative miRNA Targets on 0610030E20Rik
3'UTR of 0610030E20Rik
(miRNA target sites are highlighted)
>0610030E20Rik|NM_026696|3'UTR
   1 CGGGGCGGAGGCAGGATTCACTGCACTTGGCAGAATTTTGCCCAAGGAGCTCACCCCTAGTTTGGGGGGTGTCAGGATGA
  81 GTCCATGTGATCTTCAGGGAGCCTGTCCTCCTTCTCTAAGCTGCTGTATCCACAAAGGTCTTGCAGGACCACATCTGACC
 161 TGTCTCTCAGCAGCCATTTTTCTTGTCTCATTCATCTAACCTAACATTCTGGAATTTTATGACAGCTTTGGCTAACTTGG
 241 TGTTTTGGGAGCAGAGACTTTAGGGAGAGCCAGGCTTTCTGAAGCTTTTGCCTGTGTGGTGCTGTCTTGATGAGGTCGTT
 321 TGTCAGGCCCCTTCATGTTTCTGCTTTCTTCCACTCATCTTTGCCTAGGCCTGTTTTCAGCTGTCACTTTTCCTTTCTCC
 401 TATCTTTTTGCCTTAGGTAGAATCTATGTCCCCCACCCCCAGCATGACTGTTTCTCTGCCACTCAGGAATTTAGCATGGG
 481 ACTTCTGGGAGCCTGCCTTCCTAACAAGGTCTCCAAGTACTTCTGATGCCAATAAACAGGAGAGCTTTGGTTTTGAGGCC
 561 TCTTTGGAAGCACATGTCCTTCCCCTTTTGTGGCTTGCCCAAGCTCCTCATAGGCCTCACTGCTTAAAACTGTCTACGTA
 641 GGAGACCCAGGAAGAAGATGCTGAGCTAAGCTGCAGTCCTTAGTTGAAAAACCTGTCCTGCTACTCGTGGTGGCACATGC
 721 CTTTAGTCCCAGTACTCAGGACTCAGGAGGTAGAGATGGGTAGATCTCTGTGAGTTCAAAGCCAGCCTGGTCATATCAAC
 801 ACATCTAGTTCCAGGACAGACAGTGGTTGAGAGAGCCTATCTCAAAACAAAACAAAAAAAGAAGAAGAAGAAGAAGATGA
 881 ACCCGCCTTGGCTAGGCTTACCAAATTCACCATCTAGCTGTAAAGCAACTACCTTCTGCTCAGACTAAAGGGGAATTATC
 961 AAAGCAGTCTTAAAAGTAACTCTTCAATTGTAGTTTAGCAATCTTGAATGAATGAAGTTGAGAGAGTTGACAATAGCTAT
1041 AATTGTTCTGGGGCAGGAGGGCTCTAGTTGCAAAAAAGAGAGAGTTGTGAAGAACATCACAGAGGAAGTGAAATGGCCCT
1121 TTCTCTGCCCCATGCTTTCTTTCCTACCTCCCAAGAATATGTCTGAGCCTGATGAGACACACTTTAACCCCAGCATGAAA
1201 GAGGCCAAAGTATGAGGACTGCTGCAAGTTAAAGACCAACCTAAGCTGTCTCAAGAAAAAACTAAATAAATGTAACTTAG
1281 GTCCTTTGGGTAAAGATCCCCAGACATAGCATTTTTCCAACGGGGGACATGTTTTTCAACACAAGCTTAGGCTAAGGTCT
1361 GAAGTCCACAAGACATCCTGTTCACAGAATTCAGGTGCACCTACGATTTCCTGACATAGGAACCTAACTTCAGCATCTTG
1441 AAACATGAATCATAAAACAGCCTACAAGCCCTGAGACTGCCCACAACCAAACCTCCTACCCCAGGGCTTTGTCTTCTAGG
1521 ATTTCAAGATCATTCTGACTGTATGAGTTCAGTAGCTTTCTCTTCAGGGACACTTGCGATCCCTCCTGTGTTTTATCCCA
1601 CTCTGCTGAAAACATGTAATATAGTTGGCCATCTGCTTTACGCCATTAAAACAGTGAGTCACAGCTTGCCATGCCAGAAC
1681 TCTGGGACTTGAGTTAGGTGGTAAGTTAGAAGGGCCTGGTTCTAGAGGTGGCCGGAGACCTGAATGCTCTAAACCAGGAG
1761 AGCTGGTCTCAGGGTTCAGTGCCTTAAGCATTGTCAGTCCTAATCCCAGGATGCTGTCAAAGGGAAAAGCCTTCATCCTG
1841 TACTTGTGCACTAGTGCTGTAAGTGCCTGTGGACTTACATGGTTAGATACCATCAGAGCCTCCCGGGTGAAGGAGGGTCT
1921 GCCAAACTCCATGCTCTTCATCCTGCAAGTCACTATAGTCTCCAGTTAATCAGTCCATATTCTGACTTGAAACACAGGAT
2001 TCACTGGAACGCTGGTCGGGACAAAGAACATTGGAACTTTTTGGGGGTCTGTGTTCTTAGAGCTGTGCTTCCTATATCCT
2081 GATTCTGTTATTGAACCAAAAAACACCTGAAGAATCTGAGGACCCGACACCTACAAATTTGCCGTTATTCCCACTTCTTG
2161 ATGAAGGAATAGCTAGATGAACAAAGTGTTCTGCAGAGGCCTTTTATGTGTTTATGTGGTCCTCAAAGACCTCAGATAGG
2241 AGACTGGTGGAAACTTCTCTAAGTTCTTTTTCCAGAAGCACTTTGCTTATCAGTGTGTATGAAAGATTTTCTAGCTAAGA
2321 AAGCTCTCTTGATTCTTGCTAGTTTATCCTGTATGACTACCTAAAGCCCCAAGCCTGTGCCTAAATGAAGAGTGGTCATT
2401 CTGAGCCAGCCTGTTAGAGCTGATGTTTCTAGGCCTCCTCCCTAAAATGCCTCTTGGGGCTGCCCTCAGCCTTATGGCTT
2481 AGCTTGATTGCTCTGCTGGCCTATTCTGGGGTCTGTAATAATACTGGCCAGTTTATTTGGCAGACCTGCTTCCAGATAGA
2561 TGTCTCTTAACCTATTTCAAAACTCACGGGCCATAGTGAGTTCTCAGAACTGCCCAGTGTTTTTGCACAGTAGGCAGGAC
2641 TTAACTACAAACTGCCTCAGAATCAACATTCATTTGAATAGATAAGTTCTTATCTGTCTTTTGTGTGTGTGTGGGGGGGG
2721 GGGAGATGGGGGCAAGGGAGACTGGGCTACAGGTATGACGGTACATGCCAGTAATCCCAGCATTTGAAAGGCTGAGATAA
2801 AAGGTTTGCCCCATGTCCCAGAGGCAGGAAGGTCTTTTAGTTTGAGGCCAGCCTTATCTACAAAGCAAGTTCCAGACAAC
2881 CAGGACTATGTAGAGAGACCCTGTCTCAAACCCCTACCCCCATTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTT
2961 TCTTTCTTTCTTTCTTTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCTCCTC
3041 TCCTCTCCTCTCCTCTCCTCTCCTCTCTCTCCTCTCTCTCTCTTCTCTTCCTTCTCCTCTTCTCTCTCTCCCCTCTCTCT
3121 CTTCTCTCTCTGGAATGGTTTTTTCCCCTCAGTTCTTAACTAGAATTAAGCCTGTATTTTACAGTTTTGTGATTCCTGAA
3201 CTGAGTATATATTATTTATAAGAGAGAATTGGTTTTGCAAGGGGGTTTGGGCTGTGCAAGAATAATGATAAAAACTGGGG
3281 TTGTACCCCACTGACAAGCATGAATGACTTTGAAGAGTGTGGAGCAAGAAGGGAAAAGATATATAAGTGTATATGATGAG
3361 AATAAAGTATATACTGGCTGCAGTCTAGCCAGCAGGGGTGGATCTAAAATAAATGTGAAAGAACATTCCATCTCTCTGAT
3441 CATCTGGGGTGCATTGCTAACTAGAGAGCAGCCCAGACAGGTGGGAAGCTCTTTGGGTGGTGAGTATCTAGCCTGCTGTG
3521 TGTTTGACAAGACCTGTGTCAGGGTGGTGCTTCCCAGATAACTCGAATTTAAATGAGACCTTTGTATAATGAGCATTCGA
3601 CTTTCCTACCTCTGAGCCTTTTGAAGAAATTGCATATGGAATGTTGACAGTTATTTGAAACCTTAACTCTTTTGAATTCC
3681 TGAGGAGCTGTTGGCATCCAGCCCAAATTTCCTTGGTGTTAGAGTACTGCTAGAAGTGAAAGCCTTCCTAGTTAGGGGTT
3761 CCTCCTTTCCCAGAGCAAACAGAACAAGAGGTTGGGAATGGTGGTGAACAGCTCAAGAGACAGAGATAGGAGGATCACTG
3841 TATGTTTGAGAGTAGCCTAAGCTACACAGCAAGCATCAGCGTGTCCTGGCTATGTCATGGTGCTAAATGCAGAAAAAGCC
3921 ATACATTCTTTTTCTCATTTCCAGTGTTTCTGTATAAAAAAATGAATTATGCAAACTGTTTGAAAATCATGGACGGTACT
4001 TTTTAAACCAATAAACTATTTTTACAGCATTTTTTTGCCAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1
miRNA  3' agaUAAGUCGUACUACAGAAAg 5'
             :|||  :||  ||||||| 
Target 5' taaGTTC-TTAT-CTGTCTTTt 3'
2683 - 2702 145.00 -6.70
2
miRNA  3' agauaAGUCGU-AC----UACAGAAAg 5'
               |||| | ||    :|||:||| 
Target 5' agttcTCAGAACTGCCCAGTGTTTTTg 3'
2599 - 2625 135.00 -5.70
3
miRNA  3' agauaagUCGUAC-UACAGAAAg 5'
                 |||||| ||| |||| 
Target 5' actgacaAGCATGAATGACTTTg 3'
3290 - 3312 135.00 -12.60
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions mESCs
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM622570. RNA binding protein: AGO2. Condition:WT1A ...

- Leung AK; Young AG; Bhutkar A; Zheng GX; et al., 2011, Nature structural & molecular biology.

Article - Leung AK; Young AG; Bhutkar A; Zheng GX; et al.
- Nature structural & molecular biology, 2011
MicroRNAs (miRNAs) are 19-22-nucleotide noncoding RNAs that post-transcriptionally regulate mRNA targets. We have identified endogenous miRNA binding sites in mouse embryonic stem cells (mESCs), by performing photo-cross-linking immunoprecipitation using antibodies to Argonaute (Ago2) followed by deep sequencing of RNAs (CLIP-seq). We also performed CLIP-seq in Dicer(-)/(-) mESCs that lack mature miRNAs, allowing us to define whether the association of Ago2 with the identified sites was miRNA dependent. A significantly enriched motif, GCACUU, was identified only in wild-type mESCs in 3' untranslated and coding regions. This motif matches the seed of a miRNA family that constitutes ~68% of the mESC miRNA population. Unexpectedly, a G-rich motif was enriched in sequences cross-linked to Ago2 in both the presence and absence of miRNAs. Expression analysis and reporter assays confirmed that the seed-related motif confers miRNA-directed regulation on host mRNAs and that the G-rich motif can modulate this regulation.
LinkOut: [PMID: 21258322]
CLIP-seq Support 1 for dataset GSM622570
Method / RBP HITS-CLIP / AGO2
Cell line / Condition mESCs / WT1A
Location of target site NM_026696 | 3UTR | UUGUGUGUGUGUGGGGGGGGGGGAG
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 21258322 / GSE25310
CLIP-seq Viewer Link
101 mmu-miR-743b-3p Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT577961 Pm20d2 peptidase M20 domain containing 2 2 2
MIRT578387 Klra2 killer cell lectin-like receptor, subfamily A, member 2 2 6
MIRT579831 Zfhx3 zinc finger homeobox 3 2 8
MIRT580616 Syp synaptophysin 2 2
MIRT580731 Srrm4 serine/arginine repetitive matrix 4 2 2
MIRT581440 Rasa2 RAS p21 protein activator 2 2 2
MIRT582031 Pappa pregnancy-associated plasma protein A 2 2
MIRT582678 Lin28b lin-28 homolog B (C. elegans) 2 8
MIRT582831 Itih5 inter-alpha (globulin) inhibitor H5 2 2
MIRT583084 Hlcs holocarboxylase synthetase (biotin- [propriony-Coenzyme A-carboxylase (ATP-hydrolysing)] ligase) 2 8
MIRT583879 Dusp18 dual specificity phosphatase 18 2 6
MIRT583900 Dstyk dual serine/threonine and tyrosine protein kinase 2 4
MIRT585980 Sfxn4 sideroflexin 4 2 4
MIRT590345 Cntn2 contactin 2 2 6
MIRT591765 Ppm1f protein phosphatase 1F (PP2C domain containing) 2 4
MIRT592938 Astn2 astrotactin 2 2 2
MIRT593579 Zfp709 zinc finger protein 709 2 2
MIRT593675 Slc5a3 solute carrier family 5 (inositol transporters), member 3 2 2
MIRT593750 Peg10 paternally expressed 10 2 2
MIRT593771 Nmnat3 nicotinamide nucleotide adenylyltransferase 3 2 2
MIRT593825 Krit1 KRIT1, ankyrin repeat containing 2 2
MIRT593859 Gm5460 predicted gene 5460 2 2
MIRT593871 Galnt10 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 2 2
MIRT593891 Elovl5 ELOVL family member 5, elongation of long chain fatty acids (yeast) 2 2
MIRT593935 Cxcl3 chemokine (C-X-C motif) ligand 3 2 2
MIRT593937 Cttnbp2nl CTTNBP2 N-terminal like 2 2
MIRT594001 Aptx aprataxin 2 2
MIRT594019 Tmem241 transmembrane protein 241 1 1
MIRT594730 Zfp931 zinc finger protein 931 2 2
MIRT594766 Tmlhe trimethyllysine hydroxylase, epsilon 2 2
MIRT594836 Opa3 optic atrophy 3 2 2
MIRT594873 Magt1 magnesium transporter 1 2 2
MIRT594927 Gm14326 predicted gene 14326 2 2
MIRT594941 Fosl2 fos-like antigen 2 2 2
MIRT594980 Eda2r ectodysplasin A2 receptor 2 2
MIRT595014 Cpd carboxypeptidase D 2 2
MIRT595076 Yae1d1 Yae1 domain containing 1 1 1
MIRT595132 Lrrc4c leucine rich repeat containing 4C 2 2
MIRT595148 Epha7 Eph receptor A7 2 2
MIRT595169 Zpbp zona pellucida binding protein 2 2
MIRT595180 Tsn translin 2 2
MIRT595185 Trim25 tripartite motif-containing 25 2 2
MIRT595187 Top2a topoisomerase (DNA) II alpha 2 2
MIRT595193 Tmtc3 transmembrane and tetratricopeptide repeat containing 3 2 2
MIRT595199 Tbc1d2b TBC1 domain family, member 2B 2 2
MIRT595204 St6gal1 beta galactoside alpha 2,6 sialyltransferase 1 2 2
MIRT595210 Snx27 sorting nexin family member 27 2 2
MIRT595226 Serinc1 serine incorporator 1 2 2
MIRT595229 Sema4d sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D 2 2
MIRT595232 Sec24a Sec24 related gene family, member A (S. cerevisiae) 2 2
MIRT595235 Scn3a sodium channel, voltage-gated, type III, alpha 2 2
MIRT595242 Rc3h1 RING CCCH (C3H) domains 1 2 2
MIRT595255 Ppm1k protein phosphatase 1K (PP2C domain containing) 2 2
MIRT595259 Plxna4 plexin A4 2 2
MIRT595264 Phf3 PHD finger protein 3 2 2
MIRT595271 Nxph1 neurexophilin 1 2 2
MIRT595273 Npas3 neuronal PAS domain protein 3 2 2
MIRT595298 Kcnv1 potassium channel, subfamily V, member 1 2 2
MIRT595317 Gna14 guanine nucleotide binding protein, alpha 14 2 2
MIRT595321 Gm10318 predicted gene 10318 2 2
MIRT595328 Galnt4 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 4 2 2
MIRT595347 Frem2 Fras1 related extracellular matrix protein 2 2 2
MIRT595375 Epb4.1l4a erythrocyte membrane protein band 4.1 like 4a 2 2
MIRT595380 Dusp11 dual specificity phosphatase 11 (RNA/RNP complex 1-interacting) 2 2
MIRT595383 Dek DEK oncogene (DNA binding) 2 2
MIRT595385 Dcun1d1 DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae) 2 2
MIRT595391 Cetn3 centrin 3 2 2
MIRT595394 Cd8a CD8 antigen, alpha chain 2 2
MIRT595398 Asprv1 aspartic peptidase, retroviral-like 1 2 2
MIRT595403 Arid2 AT rich interactive domain 2 (ARID, RFX-like) 2 2
MIRT595414 Ankrd16 ankyrin repeat domain 16 2 2
MIRT595416 Anapc11 anaphase promoting complex subunit 11 2 2
MIRT595421 Akna AT-hook transcription factor 2 2
MIRT595438 9930013L23Rik cell migration inducing protein, hyaluronan binding 2 2
MIRT595441 9030617O03Rik D-glutamate cyclase 2 2
MIRT595459 Zswim6 zinc finger SWIM-type containing 6 2 2
MIRT595465 Tspan13 tetraspanin 13 2 2
MIRT595467 Tmem41b transmembrane protein 41B 2 2
MIRT595472 Sv2b synaptic vesicle glycoprotein 2 b 2 2
MIRT595475 Sgk3 serum/glucocorticoid regulated kinase 3 2 2
MIRT595480 Scaf11 SR-related CTD-associated factor 11 2 2
MIRT595485 Phox2b paired-like homeobox 2b 2 2
MIRT595490 Pgp phosphoglycolate phosphatase 2 2
MIRT595494 Nr1d2 nuclear receptor subfamily 1, group D, member 2 2 2
MIRT595511 Lrig1 leucine-rich repeats and immunoglobulin-like domains 1 2 2
MIRT595524 Grm2 glutamate receptor, metabotropic 2 2 2
MIRT595528 Foxn3 forkhead box N3 2 2
MIRT595536 Fign fidgetin 2 2
MIRT595546 Celf2 CUGBP, Elav-like family member 2 2 2
MIRT595553 Cd28 CD28 antigen 2 2
MIRT595840 Sumf1 sulfatase modifying factor 1 2 2
MIRT595953 Rab6a RAB6A, member RAS oncogene family 1 1
MIRT598445 Lix1 limb and CNS expressed 1 2 2
MIRT600750 Iws1 IWS1, SUPT6 interacting protein 2 2
MIRT601150 Bach2 BTB and CNC homology, basic leucine zipper transcription factor 2 2 2
MIRT602248 Grm1 glutamate receptor, metabotropic 1 2 2
MIRT603333 Slc35d2 solute carrier family 35, member D2 2 2
MIRT603399 Serpina1a serine (or cysteine) peptidase inhibitor, clade A, member 1A 2 2
MIRT604755 Rad51d RAD51 paralog D 1 1
MIRT605865 Atpbd4 diphthamine biosynthesis 6 2 2
MIRT606713 0610030E20Rik RIKEN cDNA 0610030E20 gene 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-743b Propranolol approved 4946 Quantitative real-time PCR heart 22847192 2012 down-regulated
miR-743b-3p (S)-3,5-dihydroxyphenylglycine (DHPG) NULL 443586 Quantitative real-time PCR mouse brain 22309833 2012 down-regulated

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