miRTarBase - #MIRT609622


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pre-miRNA Information
pre-miRNA hsa-mir-4526   
Genomic Coordinates chr18: 13611114 - 13611200
Description Homo sapiens miR-4526 stem-loop
Comment None
RNA Secondary Structure

Mature miRNA Information
Mature miRNA hsa-miR-4526
Sequence 54| GCUGACAGCAGGGCUGGCCGCU |75
Evidence Experimental
Experiments Illumina
SNPs in miRNA
Mutant ID Mutant Position Mutant Source
rs1418307303 1 dbSNP
rs1168108556 5 dbSNP
rs1050044717 6 dbSNP
rs1250280154 7 dbSNP
rs370878033 10 dbSNP
rs1008988805 19 dbSNP
rs536331678 20 dbSNP
Putative Targets

miRNA Expression profile
Human miRNA Tissue Atlas
Circulating MicroRNA Expression Profiling
Gene Information
Gene Symbol TRPC4AP   
Synonyms C20orf188, PPP1R158, TRRP4AP, TRUSS
Description transient receptor potential cation channel subfamily C member 4 associated protein
Transcript NM_015638   
Other Transcripts NM_199368   
Expression
Putative miRNA Targets on TRPC4AP
3'UTR of TRPC4AP
(miRNA target sites are highlighted)		 
>TRPC4AP|NM_015638|3'UTR
   1 CCTTGGGCCAGGCCTCGGGAGGCTGCTGGGCCAGTGTGGGTGAGCGTGGGTACGATGCCACACGCCCTGCCCTGTTCCCG
  81 TTCCTCCCTGCTGCTCTCTGCCTGCCCCAGGTCTTTGGGTACAGGCTTGGTGGGAGGGAAGTCCTAGAAGCCCTTGGTCC
 161 CCCTGGGTCTGAGGGCCCTAGGTCATGGAGAGCCTCAGTCCCCATAATGAGGACAGGGTACCATGCCCACCTTTCCTTCA
 241 GAACCCTGGGGCCCAGGGCCACCCAGAGGTAAGAGGACATTTAGCATTAGCTCTGTGTGAGCTCCTGCCGGTTTCTTGGC
 321 TGTCAGTCAGTCCCAGAGTGGGGAGGAAGATATGGGTGACCCCCACCCCCCATCTGTGAGCCAAGCCTCCCTTGTCCCTG
 401 GCCTTTGGACCCAGGCAAAGGCTTCTGAGCCCTGGGCAGGGGTGGTGGGTACCAGAGAATGCTGCCTTCCCCCAAGCCTG
 481 CCCCTCTGCCTCATTTTCCTGTAGCTCCTCTGGTTCTGTTTGCTCATTGGCCGCTGTGTTCATCCAAGGGGGTTCTCCCA
 561 GAAGTGAGGGGCCTTTCCCTCCATCCCTTGGGGCACGGGGCAGCTGTGCCTGCCCTGCCTCTGCCTGAGGCAGCCGCTCC
 641 TGCCTGAGCCTGGACATGGGGCCCTTCCTTGTGTTGCCAATTTATTAACAGCAAATAAACCAATTAAATGGAGACTATTA
 721 AATAACTTTATTTTAAAAATGAAAAAAAAAAAAAAAAAAAA
Target sites Provided by authors   Predicted by miRanda    DRVs    SNPs    DRVs & SNPs
miRNA-target interactions
(Predicted by miRanda)
ID Duplex structure Position Score MFE
1 
miRNA  3' ucGCCGGUC--GGGA-CGACAGUCg 5'
            | |||:|  :|:| |||||||| 
Target 5' tcCTGCCGGTTTCTTGGCTGTCAGt 3'
 303 - 327 162.00 -22.20
2 
miRNA  3' ucgccGGUCGGG---ACGACAGUCg 5'
               ||| |||   | |||| || 
Target 5' gacccCCACCCCCCAT-CTGTGAGc 3'
 358 - 381 120.00 -16.50
3 
miRNA  3' ucgcCGGU---CGGGAC--GACAGucg 5'
              ||||   ||||||  ||||:   
Target 5' cgatGCCACACGCCCTGCCCTGTTccc 3'
 53 - 79 112.00 -19.40
DRVs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
COSN18743399 17 COSMIC
COSN30185021 17 COSMIC
COSN31610147 26 COSMIC
COSN31531889 30 COSMIC
COSN31610696 45 COSMIC
COSN30192936 46 COSMIC
COSN30473433 48 COSMIC
COSN27000128 53 COSMIC
COSN31559945 55 COSMIC
COSN27000126 64 COSMIC
COSN30470783 78 COSMIC
COSN31538330 79 COSMIC
COSN31510332 88 COSMIC
COSN9126583 96 COSMIC
COSN30103619 108 COSMIC
COSN30190226 119 COSMIC
COSN30140657 162 COSMIC
COSN30540276 171 COSMIC
COSN30540291 172 COSMIC
COSN30173719 221 COSMIC
COSN10014805 337 COSMIC
COSN31609119 374 COSMIC
COSN17183251 384 COSMIC
COSN26649112 441 COSMIC
COSN26670708 447 COSMIC
COSN31482780 498 COSMIC
COSN31485470 682 COSMIC
COSN31779295 736 COSMIC
COSN31779294 737 COSMIC
COSN15658125 741 COSMIC
COSN21515193 741 COSMIC
SNPs in gene 3'UTRs
Mutant ID Mutant Position Mutant Source
rs369840973 1 dbSNP
rs1483813740 3 dbSNP
rs1176959354 5 dbSNP
rs773063754 6 dbSNP
rs771931893 9 dbSNP
rs748206967 11 dbSNP
rs778834984 14 dbSNP
rs755124998 16 dbSNP
rs749517265 17 dbSNP
rs780481547 18 dbSNP
rs755534950 20 dbSNP
rs199725379 34 dbSNP
rs1280207892 36 dbSNP
rs928884301 36 dbSNP
rs1436472127 37 dbSNP
rs185046445 39 dbSNP
rs376635360 42 dbSNP
rs754117468 42 dbSNP
rs373376388 45 dbSNP
rs757734177 46 dbSNP
rs549449754 53 dbSNP
rs554330546 54 dbSNP
rs1382749271 58 dbSNP
rs201093403 63 dbSNP
rs963092700 64 dbSNP
rs1009718440 77 dbSNP
rs1016007774 79 dbSNP
rs1007330988 80 dbSNP
rs1032510033 94 dbSNP
rs1285073281 94 dbSNP
rs1217133190 100 dbSNP
rs532924845 102 dbSNP
rs1425859985 104 dbSNP
rs1030178271 105 dbSNP
rs1287720853 106 dbSNP
rs1427863596 106 dbSNP
rs1168153022 107 dbSNP
rs1356094997 110 dbSNP
rs1430115406 113 dbSNP
rs1302204996 120 dbSNP
rs905404821 122 dbSNP
rs140848050 123 dbSNP
rs903103160 126 dbSNP
rs1274938383 138 dbSNP
rs1360579047 144 dbSNP
rs1355744906 146 dbSNP
rs1284783831 148 dbSNP
rs1013559225 153 dbSNP
rs1416883853 165 dbSNP
rs1338643684 167 dbSNP
rs1291400029 171 dbSNP
rs747364720 177 dbSNP
rs1489917196 184 dbSNP
rs1041705874 192 dbSNP
rs887091748 193 dbSNP
rs1247370625 194 dbSNP
rs1011617491 196 dbSNP
rs931384581 204 dbSNP
rs1184733577 208 dbSNP
rs192559769 211 dbSNP
rs1362835354 222 dbSNP
rs1449897268 224 dbSNP
rs893222411 231 dbSNP
rs899036170 234 dbSNP
rs1055910924 245 dbSNP
rs1426180988 246 dbSNP
rs1163313383 251 dbSNP
rs1323756306 252 dbSNP
rs1037464739 259 dbSNP
rs1405224458 266 dbSNP
rs796355258 267 dbSNP
rs1287684817 269 dbSNP
rs1164442190 275 dbSNP
rs201797237 281 dbSNP
rs1314801963 283 dbSNP
rs571593554 283 dbSNP
rs1045915701 286 dbSNP
rs910446670 290 dbSNP
rs1262073402 294 dbSNP
rs930178835 295 dbSNP
rs1193926730 298 dbSNP
rs1213700714 299 dbSNP
rs987556343 300 dbSNP
rs1244706431 301 dbSNP
rs550121954 309 dbSNP
rs562462939 310 dbSNP
rs971704326 315 dbSNP
rs1473634968 316 dbSNP
rs1163525786 321 dbSNP
rs1406764095 322 dbSNP
rs1290703656 325 dbSNP
rs957767976 333 dbSNP
rs987949999 333 dbSNP
rs1388644057 336 dbSNP
rs1032513218 343 dbSNP
rs1235266232 345 dbSNP
rs550598666 351 dbSNP
rs908954344 353 dbSNP
rs1381081275 362 dbSNP
rs1450593367 364 dbSNP
rs8501 365 dbSNP
rs1219422315 370 dbSNP
rs1270112612 371 dbSNP
rs1025251145 372 dbSNP
rs150022394 372 dbSNP
rs1261147249 373 dbSNP
rs375732962 375 dbSNP
rs527663000 375 dbSNP
rs1187986088 377 dbSNP
rs1292109317 378 dbSNP
rs112617727 379 dbSNP
rs1468285291 381 dbSNP
rs1014235859 384 dbSNP
rs535151593 385 dbSNP
rs1412094920 398 dbSNP
rs1436422468 413 dbSNP
rs548344978 423 dbSNP
rs14206 425 dbSNP
rs1176385578 426 dbSNP
rs1400660478 431 dbSNP
rs530259400 435 dbSNP
rs1335264953 439 dbSNP
rs1328480668 441 dbSNP
rs559679603 443 dbSNP
rs997367050 444 dbSNP
rs766342417 446 dbSNP
rs967692328 448 dbSNP
rs1351231096 450 dbSNP
rs1170964016 453 dbSNP
rs1478559334 460 dbSNP
rs1347032462 469 dbSNP
rs1200899483 478 dbSNP
rs1272042099 478 dbSNP
rs1435815529 479 dbSNP
rs991348690 480 dbSNP
rs541259359 482 dbSNP
rs1479851317 484 dbSNP
rs532642040 487 dbSNP
rs1196629113 493 dbSNP
rs1037516126 499 dbSNP
rs1479290323 502 dbSNP
rs1173608724 505 dbSNP
rs1020252411 523 dbSNP
rs1396271244 523 dbSNP
rs1190359079 525 dbSNP
rs565207301 526 dbSNP
rs1488670896 527 dbSNP
rs1058003 532 dbSNP
rs575903522 533 dbSNP
rs1332848431 535 dbSNP
rs1233907544 547 dbSNP
rs1269482243 551 dbSNP
rs1418726121 560 dbSNP
rs1337249231 583 dbSNP
rs554597666 585 dbSNP
rs893170125 586 dbSNP
rs1274976366 590 dbSNP
rs4616 591 dbSNP
rs1001999683 592 dbSNP
rs1199545706 596 dbSNP
rs907285469 598 dbSNP
rs1046313856 607 dbSNP
rs1299439769 610 dbSNP
rs1448114181 612 dbSNP
rs1391100500 620 dbSNP
rs913603106 620 dbSNP
rs867189701 621 dbSNP
rs1368744717 622 dbSNP
rs572141509 625 dbSNP
rs1387394307 627 dbSNP
rs1317612752 629 dbSNP
rs146419473 635 dbSNP
rs941631147 636 dbSNP
rs980501404 639 dbSNP
rs1243341896 643 dbSNP
rs1463216809 649 dbSNP
rs1357114439 650 dbSNP
rs969568156 653 dbSNP
rs760595520 656 dbSNP
rs750211451 660 dbSNP
rs1174874159 664 dbSNP
rs540562330 668 dbSNP
rs1261722822 669 dbSNP
rs1463939642 677 dbSNP
rs1190997927 685 dbSNP
rs1433623133 690 dbSNP
rs1162997537 691 dbSNP
rs1426940169 691 dbSNP
rs1370393102 692 dbSNP
rs1458526186 699 dbSNP
rs1025560697 700 dbSNP
rs992456817 700 dbSNP
rs951680868 701 dbSNP
rs1438968770 708 dbSNP
rs1237671553 710 dbSNP
rs1394050282 714 dbSNP
rs1025648342 718 dbSNP
rs1340698024 719 dbSNP
rs995444659 720 dbSNP
rs1258449201 721 dbSNP
rs1330544493 722 dbSNP
rs773485942 726 dbSNP
rs1018342738 727 dbSNP
rs371284276 728 dbSNP
rs538809046 729 dbSNP
rs541948497 729 dbSNP
rs1181283657 730 dbSNP
rs1424616807 730 dbSNP
rs1409873458 731 dbSNP
rs1254556454 734 dbSNP
rs1159887377 736 dbSNP
rs571483235 741 dbSNP
Experimental Support 1 for Functional miRNA-Target Interaction
miRNA:Target ----
Validation Method
     
Conditions HEK293S
Location of target site 3'UTR
Tools used in this research TargetScan , miRTarCLIP , Piranha
Original Description (Extracted from the article) ... HITS-CLIP data was present in GSM1084080. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep2_SantaCruzAb HITS-CLIP data was present in GSM1084083. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep2_SigmaAb ...

- Karginov FV; Hannon GJ, 2013, Genes & development.

miRNA-target interactions (Provided by authors)
ID Duplex structure Position
1 
miRNA  3' ucgccggucgggacgACAGUCg 5'
                         |||||| 
Target 5' ---------------UGUCAGu 3'
 		1 - 7
Article - Karginov FV; Hannon GJ
- Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
CLIP-seq Support 1 for dataset GSM1084083
Method / RBP HITS-CLIP / AGO2
Cell line / Condition HEK293S / CLIP_hippuristanol_rep2_SigmaAb
Location of target site ENST00000451813.2 | 3UTR | UGUCAGUCAGUCCCAGAGUGGGGAGGAAGAU
Tools used in this analysis TargetScan, miRTarCLIP, and Piranha
Article / Accession Series PMID: 23824327 / GSE44404
CLIP-seq Viewer Link
MiRNA-Target Expression Profile
Dataset Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
MiRNA-Target Expression Profile (TCGA)
Tumor Pearson Correlation P-value for Pearson Correlation Spearman Correlation P-value for Spearman Correlation Samples Chart
60 hsa-miR-4526 Target Genes:
Functional analysis:
ID Target Description Validation methods
Strong evidence Less strong evidence
MIRT080216 PRKACB protein kinase cAMP-activated catalytic subunit beta 2 2
MIRT100350 DDX39B DExD-box helicase 39B 2 2
MIRT445480 KDM6A lysine demethylase 6A 2 2
MIRT450233 ZNF25 zinc finger protein 25 2 2
MIRT465783 TMOD3 tropomodulin 3 2 8
MIRT466253 TMBIM6 transmembrane BAX inhibitor motif containing 6 2 2
MIRT470395 PPP1R16B protein phosphatase 1 regulatory subunit 16B 2 2
MIRT470442 PPP1R15B protein phosphatase 1 regulatory subunit 15B 2 8
MIRT476323 GLTSCR1L BRD4 interacting chromatin remodeling complex associated protein like 2 2
MIRT479706 CCNT1 cyclin T1 2 2
MIRT481925 ANKRD33B ankyrin repeat domain 33B 2 2
MIRT484016 ZNF776 zinc finger protein 776 2 4
MIRT497022 INO80B INO80 complex subunit B 2 2
MIRT502526 EPHA2 EPH receptor A2 2 4
MIRT513497 SSR1 signal sequence receptor subunit 1 2 6
MIRT517846 RPS4X ribosomal protein S4, X-linked 2 4
MIRT533734 TMEM200C transmembrane protein 200C 2 2
MIRT535904 MKNK2 MAP kinase interacting serine/threonine kinase 2 2 2
MIRT537537 EZR ezrin 2 2
MIRT538756 CACUL1 CDK2 associated cullin domain 1 2 2
MIRT540307 GFPT1 glutamine--fructose-6-phosphate transaminase 1 2 2
MIRT545510 EPT1 selenoprotein I 2 2
MIRT548829 CHIC1 cysteine rich hydrophobic domain 1 2 2
MIRT569600 TRIM29 tripartite motif containing 29 2 2
MIRT572462 ZNF516 zinc finger protein 516 2 2
MIRT575457 Ints2 integrator complex subunit 2 2 3
MIRT609411 SLC25A45 solute carrier family 25 member 45 2 2
MIRT609622 TRPC4AP transient receptor potential cation channel subfamily C member 4 associated protein 2 2
MIRT609666 INTS2 integrator complex subunit 2 2 3
MIRT609994 PIGS phosphatidylinositol glycan anchor biosynthesis class S 2 2
MIRT611691 NODAL nodal growth differentiation factor 2 2
MIRT615720 BACE2 beta-site APP-cleaving enzyme 2 2 2
MIRT616383 C1orf87 chromosome 1 open reading frame 87 2 2
MIRT616593 KLHL9 kelch like family member 9 2 2
MIRT628162 HIP1 huntingtin interacting protein 1 2 2
MIRT628376 CAMK2N1 calcium/calmodulin dependent protein kinase II inhibitor 1 2 2
MIRT629203 PAPOLA poly(A) polymerase alpha 2 2
MIRT635006 ADPRH ADP-ribosylarginine hydrolase 2 2
MIRT645493 TRIM63 tripartite motif containing 63 2 2
MIRT646750 MUC4 mucin 4, cell surface associated 2 2
MIRT649613 ITPKC inositol-trisphosphate 3-kinase C 2 2
MIRT656228 MFSD6 major facilitator superfamily domain containing 6 2 2
MIRT658196 FBXO44 F-box protein 44 2 2
MIRT661757 MICA MHC class I polypeptide-related sequence A 2 2
MIRT662866 UPF3A UPF3A, regulator of nonsense mediated mRNA decay 2 2
MIRT666738 RALY RALY heterogeneous nuclear ribonucleoprotein 2 2
MIRT690391 PARP15 poly(ADP-ribose) polymerase family member 15 2 2
MIRT699795 SEC24A SEC24 homolog A, COPII coat complex component 2 2
MIRT700033 RPL22 ribosomal protein L22 2 2
MIRT701741 MTDH metadherin 2 2
MIRT702330 KMT2A lysine methyltransferase 2A 2 2
MIRT702672 IRS2 insulin receptor substrate 2 2 2
MIRT708730 LSAMP limbic system-associated membrane protein 2 2
MIRT709230 GPSM2 G protein signaling modulator 2 2 2
MIRT717503 UFL1 UFM1 specific ligase 1 2 2
MIRT718533 PIGQ phosphatidylinositol glycan anchor biosynthesis class Q 2 2
MIRT719845 MON1B MON1 homolog B, secretory trafficking associated 2 2
MIRT720957 TMEM151B transmembrane protein 151B 2 2
MIRT724816 MSX2 msh homeobox 2 2 2
MIRT725369 MTF2 metal response element binding transcription factor 2 2 2
miRNA-Drug Associations
miRNA Small Melocule FDA CID Detection Method Condition PMID Year Expression Pattern of miRNA
miR-4 Dexamethasone approved 5743 Microarray primary rat thymocytes 20847043 2010 up-regulated
miRNA-Drug Resistance Associations
miRNA Drug Name CID NSC FDA Effect/Pattern Detection Method Level Phenotype Condition
hsa-mir-4526 Cisplatin 5460033 NSC119875 approved resistant cell line (BxPC3)
hsa-miR-4526 Gefitinib 123631 NSC715055 approved resistant cell line (PC9)
hsa-miR-4526 Osimertinib 71496458 NSC779217 approved resistant cell line (PC9)
hsa-miR-4526 Platinum 23939 resistant tissue (non-small cell lung cancer)
hsa-miR-4526 Gemcitabine 60750 NSC613327 approved sensitive cell line (PANC-1) (100 ng/ml)

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