pre-miRNA Information | |
---|---|
pre-miRNA | hsa-mir-4457 |
Genomic Coordinates | chr5: 1309310 - 1309377 |
Description | Homo sapiens miR-4457 stem-loop |
Comment | None |
RNA Secondary Structure | ![]() |
Mature miRNA Information | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mature miRNA | hsa-miR-4457 | |||||||||||||||||||||
Sequence | 43| UCACAAGGUAUUGACUGGCGUA |64 | |||||||||||||||||||||
Evidence | Experimental | |||||||||||||||||||||
Experiments | Illumina | |||||||||||||||||||||
SNPs in miRNA |
|
|||||||||||||||||||||
Putative Targets |
Gene Information | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Gene Symbol | SLC38A1 | ||||||||||||||||||||
Synonyms | ATA1, NAT2, SAT1, SNAT1 | ||||||||||||||||||||
Description | solute carrier family 38 member 1 | ||||||||||||||||||||
Transcript | NM_001077484 | ||||||||||||||||||||
Other Transcripts | NM_030674 | ||||||||||||||||||||
Expression | |||||||||||||||||||||
Putative miRNA Targets on SLC38A1 | |||||||||||||||||||||
3'UTR of SLC38A1 (miRNA target sites are highlighted) |
>SLC38A1|NM_001077484|3'UTR 1 AACCCGCCGAGAAAAAGAAACATCCCTGTTGTCTGCTCAGTCAAGTCCCCACACATCAGCAATCTCTCACCACTTCTTTT 81 GCAAGTTTACAGAAGCAAACAGAAATGTACAGGATACTTAAAATGGAATAACTTTTTGGTTGCAAAACAGAGACATGGTT 161 CTATAATGCTTCATGTCCCTCCAAGATTTGAGATCAATTTAGGGATTGTGAAATTTTTTTTTCAAATTTCATACAATCAT 241 ATTTCCCAGTACTTTTCACAATCATTTTTTACCCATCTAACTCTATGTTTTGTGGCTTCCCGGTCTCTTAGAACTTTGAA 321 AACATGATATACAATAATGTTTATTTATTATACATCCAGATTCTGAAATAATTTTCCTACTGATGTTCAGCTCACACTAT 401 CTGTACCTTTTTAGAAGAGAAAAGAATCTTGAATTGTATATATTTATTTTGCTTTACAGAAAAAAATGGTTTCGTAAATA 481 ATTTGCCTATTTTGGTTAACATAGCACATGGAGATAATCATCTGAAAGTTATAGGGCACTGCCACTGCTGAATCAGAGCA 561 TGCCCAATATTTGAGGTGGCTCTGATTTCCTGGCAGCTGAACTCGGGTAGTCCAGTGGCCTAGCTGGTACCACATCTATT 641 CCCATCCAGAGACATTCTCTGGCAAGTGTTCTCAGCTGAAAAGTGGTTGGGGATGATTCTTACCTTGGTAATTAAATGAA 721 GCTACACATTTGGGTAATCTAGCAAATGAAGTATTTTTTCCCTCTTGGCAACTTGTGTCAGAGTTACTCTGGTCTGAGTC 801 AACTTTCGCTGGGGAAAACCTATGGAACCTACTGCAAAAAGATTGTCCAAAATGCCTAAGAAAATACTCCTCTGATGCAT 881 TTAGCCTTCAACCCTACCTGTCTTGCTGAAGGGAGAAAAATGTTTTAGTACATTATAGGCCCAGCAGCTTTTATTCATGT 961 CCACCAGCTAGTTGCACAGAGAATCATGTGTACCTAACTAAGGATGATCTAGGATAAGTAACTCCTGTTTTATATTGAGT 1041 ATTTTAGGGAAGTCTTTAAAAGACTTGTTTTATATCTATAAATCTAGGTTATTACAAATACAAGAATTTTGTACCTTAAA 1121 TAAGCCTCATTTCTATTTCTTCTTCATTAATTCTCCATCTAGTCTTGTGAAAAAAAAAAAAAAAAAACCCTCAGAGATAG 1201 TCTTTGTGAAGAGCTTCTGACAGAATCACTGAGTACCTTCCTTCCCCCAGATGAGGAAGACAAGGGGGTCTCAGTGTCTG 1281 TGCTGTCTCCTCTTCTCTTCCCCAACCAAGGACTGTGCCATTACTGCCCGTCTCAACTGTCCATGCAGGAGGACAGAGTT 1361 GCCTGGTACTCTTACCCTTGTCCCTCTCCTAAAGGGAGCACAAGGAAACTGAAGAGACTGAAAAAGAAGAGAGTTTGTAG 1441 CTGAAAAAGAATAGGGATAGCAAGGAAACCCAGAACTGCATTCCCCTAAGTGGGGCCATCCCATGTGATTGAATTGTCCA 1521 TAGCTTGCCTATGGTGAGAAATGTGCATGCTCCGTGAGCTGGTCTCTTGAAACAGGACTTATGCTTCCTCTATATTCTGG 1601 TTAAATTTTCCAAACACATAAGTTCACTGAGCACAGATTTCTTATCCAGAGACAAGTAGAATCTAACCGCAGACTGTTGG 1681 CAGAGTTTCCAGGCACTTAGCCATGTTCCCTTCCTGACTCAAATCCCCAAAGGCCTTCACTCTCACTGAGAATCACACTA 1761 CTGTCCCATAGATAAGGCAGGCATTGAAGCACCTGTCGTGATCCTCTAGGGGGGAGAATGAAAGGTTATTTCCTGCATTG 1841 CATCATCATAGCTTTTAATATAATGCTACAGAATCATATCCACATTAGGTTAGAGTTCAGATATTTGGATATGAATACCT 1921 AACCTAGCCATATCCATGGCCATCTCTGTTCTTTTCAGCAATGTTTTCCATATTATATTAGCAATGACAGAAACAGAACA 2001 AGCCAAGATCCAGTCAGTTCTTGGGAGCTTGTCTAGAGCACCAAGTAATGAAATAGCCAGGTAGTGGGATGACTGTACCT 2081 TTAAAAATACATAATTTAGTTTGCAAGCTATATTATGCTACTTTCTATTTTCCTTGTTACTTTATAGCAATTCATTTTAC 2161 CCTCACAAAGTCAATTTAGAACCTTATCATTAACTGGGATGTGTAGTGATATTTTTGGGCCTCTGGGTTTCATGTGTCAA 2241 TACAAGGAATATTTATTTAAAATAGATTTATTTAGAGGAGGCACAGTGTTGTTGATCTGTGTGACACCACCCATATTTTT 2321 AAAAACCTTTGTATGTTTCTCTAAATTTGTTGTTGACTGAATATAATAGACCCTACCATAATTCGTCAAATATCACTGAT 2401 TAGTTACATCCTTTGTGTGAGATTAGCTGTAAAGTATACTGCTCTTATTCTTATTCAGAATAGTTAATTGGTAGCCAAAA 2481 ATACATGTATCACAGATGTTAGGTCGAATTTAAACAGCACAGTCAAGTGCTATGGAAGTTTTTCTGCTAAATTAGTAGAT 2561 TAAAGAATACTATACCCTAGGCATGGGGAGCAGCACGTTTTCCTTTGGTAGGTAGGATCTCTATACTAGTGAACAGTGCC 2641 AGTTCCACACTTTGGACTTAGAACTGTTCTCTAGTTATTGTAACACAGAATACTGTCAATCCCTAATTTACTTAATGTTA 2721 CTTATTGGAAGTGGGGCTGATGAAATACGCACAGGAGGGAAATCTACTGTGTTTAGGCACAGGCAGCCCCAGTGTATAAG 2801 GAGATCATATTCCAAAAGGTTGTCAGTTGGTTGTTTGCAACCTGGAATGTATTTTCCTTTAGAGACCAGGTTATCCATGG 2881 TGGTTAGGCCCCTAGAGCAGCTGGAAAAGATGATCAAACCAATAGGTTAGCTGACATCGAATAATGTAATAGGTTTGCTA 2961 AAGAATCTAACCATCAAATATAATATTGTTTCCAGGGAGGGTGTTTGTTCAGAGTTGCCTGTTAGTAGAATCTGGACTGT 3041 CCATCCCAGCCACATCCCACCTACTGGACAGTAGGGGTAGAGATGCCACCAGACTACAGGTGACCAGATTGGCCTGAAAC 3121 ATGGTGTCCAGTAAAAACGGGGAGGAATAAGTCCATGACTGCTGGAGAGCTTGGTGGTAGGAGGAGGGTGAAGGAGGGTG 3201 AGGGGGCCTCTCATGGGTCAGAAACCTCCAGGGACATCCCTCAGCTGGTAACTCTGCTGTTGTCCGGAGGGTTCAGGCTT 3281 GGTGTGCCTTCTTCTGTTTGTCTGACTTTTGGCTCCTATGGTTCATCTCCTGCCCTGCCCACCACAGAAAAGGATGCTGC 3361 TGCATAGCTTCTCCAATGTACCAGTCATTGAGGCCAGCGCGCAGCACTTTTAATGTTTTTAGTGCTAGTAACTGTGTTTT 3441 AACTCTCCAGGAATAAGCTGGGAGGTTAGAAAACAAGAAAAAAGGGGGAAAAAAAAACCTCTCCACCCACTTTCCTTTTT 3521 TACAGTTACAGACCCTGTTGAGAAAAAGAAGCACCCAGATGGGTGATGGTGATAGGGCTTGTGGTGGACACAGGGTTGTG 3601 GTAAGATTCCTCAGTGCTTCGGCGAGCACCTAAGACTATTACCCTGGAACTGACTGCTTTCTAGAGCTTGATTCATGCCT 3681 CTAAGCCAGTGGTTCTCAAAGTGTGGCCCCTAGACCAGTAGCATCAGCATCATCGGGGACCTCATTAGAATGCAGATTCT 3761 CAGCCCCCTCCCAGACATGGTGAAGTCAGAAGCTCTCAGGGAGGGACTCAGCAAATGATTCTAAAGCGCATCAAGTTTGA 3841 GAACCACCCTAAGCCAATGAGTCTCCCTTTTACTTCCCATTAGTCCTCCCTCACCAGATGATTCCTAGGACTCATGGTGC 3921 AACATACAGGAGCCAGGCTTTAGGGAGGGGAGGAAAGGAGCCTGGCGGCAGGGGTCTCTGCAATGGCAAGAAAGTGAGTG 4001 AATCCAGCGCTGCCACCTGGTTATCCCCTTCTCGTTGAAAATAGCAGCGCAGGTGCAAATTCCTAAACACAGGCTGCCTG 4081 CACTCGGTGTTACCTCGGGCTGAGGCATGATAATATTTTATTTTTTAAAGCTGTGAGGAAATGAAAGTGAGGCTTTGGTT 4161 GGGGCGAGGCAGTTGTGAAAGAAATTAAAATAAAAGACCCTTTGTAAATGCAGACTTAGAAGAAATACTGAATTTGTGTC 4241 AGAAGTTCTCCAGTGTTTGTGTTAATCGTGTGGTGATAATCCTGTCCTCCTTTTAAAGCGAATTCTCTACTGAAAGGTCT 4321 GCTCTGCTTAAGGAGCTACAAACTGCTCTCAAAAGAATGAAATACTGAGTTCCAATTCAGTGAGGCACAGTGTTGGACTA 4401 TGGCACATTTAGTTGGAGTCGGGGGGAGGTCAGGAATATGATCAGATAATGGATTTTATACCTTAGAGCAAAATCTATTA 4481 GTCTCTCTCAGTTTATCAATTTAAATGGCTTTAGGCTTATAGGGGGTGTAAACTTTAAGAATATAATTCTCCCATTCAAG 4561 TTTACAGCAAACATCTAGCCACCTTCAAAACAAAGAATATACAGACCATCATTTAGCAATACTAATACATGATTTTCCTT 4641 GGGGATGGCAGGTTTGAGAATCCTTTAGCAACAGGACATACTTCCCCTAAATTACAGTGAATTATTTATAACGAGATAAA 4721 GCTTTCAGGTACAAGCTGAAGGTGGGGTGTCTAACAACTAAAAACTATCACTAAATCTCAAAGAGAAAGTTCTTGCAAAA 4801 TATGTAAAGTTCACAAGGTGCAGACATTTTCCTTCTTTAGGCTTTTATCTAAGGAAGGGCTATGAAACTGGGCCCATCTG 4881 TATACAGGCTCAAATTTACGTTTTTAAAGGAAGAATCTATGCAGCTGAGGCTTATTGCAGGAAATACTTCATTTGTATGT 4961 AAATATTATTGTAAATAAATAGGAGGCTGTATATTTTTGAAGTTGTTGATCTGCACTGAAATAGAAGTCTCTAGGATCTG 5041 CATATAAACAATAAATGTTTCCTAGAAATTAGTGGTTTTGTTTGGGAATTAGAAAAATTTACATTCTCTCCCAGGTAACA 5121 TAGTTCTCTCAATGTAAACTTGGAACCTGAAACCCTACTTAATTTAGAGAAAGAAATGTCTGAGAAATAGTTCCCTTGAT 5201 TTCTTATGCTGGCATTAAGATCAATTATTTAACAGATAGTCCTCTGAGATACAAGTAAGGAATATTTAGACACAAATCCT 5281 TGTCCTATAGAAACAGATATACCCAAATGACAAATTGTTTCTTCATAATGATCTTCCATTTTTAACACTGATACCACTAC 5361 ACAGTACATATGAAAACAGAAGCTGGGGAGAAGAATGTTTTTTTCACAATTGAATTGCTGCATTTCTCAAACTTTGGGAT 5441 CTATGAAAGCGGGGAGAGGGAACCTGAATATTAATTATGAGCACAAATTTGAAGGAAAGAAAACAAAGAACCATTATCTA 5521 ATCAAGCTTTGAAAGTCCTGCATGTTTGCCTTTTATTTTAGTGTTGACGCCAACATAGACTGTCTAAGGTATTTTTTTCC 5601 CCAAACACTTGAATCTTGGTCGTTGGTATGTAATCCACTCTCTAGAGTCCAGTGTACTTTAGACTTCATCTGAGTCCAAT 5681 ACATGTACCACACTACTGTTTTATTAATGTAAAAACCTTGTAAATGAATTTCAGATGGGTGATTTAAGTGAGTCACAAGT 5761 CACAAAACTTTGCTATTCATAGTTAATCAAATAGAACTGGGTTTTTTTTTTCAGAGTGTGGTGTAAATAAAGAAATATAA 5841 GAAGTTCTGTTCTATAACTGCTCTGTTAACATAGTTTTTAAACATTAAAAAATGTGAACTAAAAGTATTTAAAAA Target sites
Provided by authors
Predicted by miRanda
DRVs
SNPs
DRVs & SNPs
|
||||||||||||||||||||
miRNA-target interactions (Predicted by miRanda) |
|
||||||||||||||||||||
DRVs in gene 3'UTRs | |||||||||||||||||||||
SNPs in gene 3'UTRs |
Experimental Support 1 for Functional miRNA-Target Interaction | |
---|---|
miRNA:Target | ---- |
Validation Method |
|
Conditions | HeLa |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in Chi_ControlB_2A8_130_50. RNA binding protein: AGO. Condition:HeLa cell Control B
... - Chi SW; Zang JB; Mele A; Darnell RB, 2009, Nature. |
Article |
- Chi SW; Zang JB; Mele A; Darnell RB - Nature, 2009
MicroRNAs (miRNAs) have critical roles in the regulation of gene expression; however, as miRNA activity requires base pairing with only 6-8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. Recently, high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP) has identified functional protein-RNA interaction sites. Here we use HITS-CLIP to covalently crosslink native argonaute (Ago, also called Eif2c) protein-RNA complexes in mouse brain. This produced two simultaneous data sets-Ago-miRNA and Ago-mRNA binding sites-that were combined with bioinformatic analysis to identify interaction sites between miRNA and target mRNA. We validated genome-wide interaction maps for miR-124, and generated additional maps for the 20 most abundant miRNAs present in P13 mouse brain. Ago HITS-CLIP provides a general platform for exploring the specificity and range of miRNA action in vivo, and identifies precise sequences for targeting clinically relevant miRNA-mRNA interactions.
LinkOut: [PMID: 19536157]
|
Experimental Support 2 for Functional miRNA-Target Interaction | |
---|---|
miRNA:Target | ---- |
Validation Method |
|
Conditions | HEK293S |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM1084041. RNA binding protein: AGO2. Condition:CLIP_arsenite_rep1
HITS-CLIP data was present in GSM1084044. RNA binding protein: AGO2. Condition:CLIP_noarsenite_rep3
HITS-CLIP data was present in GSM1084045. RNA binding protein: AGO2. Condition:CLIP_arsenite_rep3
HITS-CLIP data was present in GSM1084046. RNA binding protein: AGO2. Condition:CLIP_noarsenite_rep4
HITS-CLIP data was present in GSM1084047. RNA binding protein: AGO2. Condition:CLIP_arsenite_rep4
HITS-CLIP data was present in GSM1084064. RNA binding protein: AGO2. Condition:CLIP_noemetine_AbnovaAb
HITS-CLIP data was present in GSM1084065. RNA binding protein: AGO2. Condition:CLIP_emetine_AbnovaAb
HITS-CLIP data was present in GSM1084066. RNA binding protein: AGO2. Condition:CLIP_noemetine_SantaCruzAb
HITS-CLIP data was present in GSM1084068. RNA binding protein: AGO2. Condition:CLIP_noemetine_SigmaAb
HITS-CLIP data was present in GSM1084069. RNA binding protein: AGO2. Condition:CLIP_emetine_SigmaAb
HITS-CLIP data was present in GSM1084074. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep1_SantaCruzAb
HITS-CLIP data was present in GSM1084075. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep1_SantaCruzAb
HITS-CLIP data was present in GSM1084076. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep1_SigmaAb
HITS-CLIP data was present in GSM1084079. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep2_AbnovaAb
HITS-CLIP data was present in GSM1084080. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep2_SantaCruzAb
HITS-CLIP data was present in GSM1084082. RNA binding protein: AGO2. Condition:CLIP_nohippuristanol_rep2_SigmaAb
HITS-CLIP data was present in GSM1084083. RNA binding protein: AGO2. Condition:CLIP_hippuristanol_rep2_SigmaAb
... - Karginov FV; Hannon GJ, 2013, Genes & development. |
Article |
- Karginov FV; Hannon GJ - Genes & development, 2013
When adapting to environmental stress, cells attenuate and reprogram their translational output. In part, these altered translation profiles are established through changes in the interactions between RNA-binding proteins and mRNAs. The Argonaute 2 (Ago2)/microRNA (miRNA) machinery has been shown to participate in stress-induced translational up-regulation of a particular mRNA, CAT-1; however, a detailed, transcriptome-wide understanding of the involvement of Ago2 in the process has been lacking. Here, we profiled the overall changes in Ago2-mRNA interactions upon arsenite stress by cross-linking immunoprecipitation (CLIP) followed by high-throughput sequencing (CLIP-seq). Ago2 displayed a significant remodeling of its transcript occupancy, with the majority of 3' untranslated region (UTR) and coding sequence (CDS) sites exhibiting stronger interaction. Interestingly, target sites that were destined for release from Ago2 upon stress were depleted in miRNA complementarity signatures, suggesting an alternative mode of interaction. To compare the changes in Ago2-binding patterns across transcripts with changes in their translational states, we measured mRNA profiles on ribosome/polysome gradients by RNA sequencing (RNA-seq). Increased Ago2 occupancy correlated with stronger repression of translation for those mRNAs, as evidenced by a shift toward lighter gradient fractions upon stress, while release of Ago2 was associated with the limited number of transcripts that remained translated. Taken together, these data point to a role for Ago2 and the mammalian miRNAs in mediating the translational component of the stress response.
LinkOut: [PMID: 23824327]
|
Experimental Support 3 for Functional miRNA-Target Interaction | |
---|---|
miRNA:Target | ---- |
Validation Method |
|
Conditions | Cardiac Tissues |
Location of target site | 3'UTR |
Tools used in this research | TargetScan , miRTarCLIP , Piranha |
Original Description (Extracted from the article) |
...
HITS-CLIP data was present in GSM2202479. RNA binding protein: AGO2. Condition:S4_LV_29yo_Male_AGO2_bound_RNA
HITS-CLIP data was present in GSM2202477. RNA binding protein: AGO2. Condition:S2_LV_25yo_Male_AGO2_bound_RNA
... - Spengler RM; Zhang X; Cheng C; McLendon JM; et al., 2016, Nucleic acids research. |
Article |
Elucidation of transcriptome-wide microRNA binding sites in human cardiac tissues by Ago2 HITS-CLIP.
- Spengler RM; Zhang X; Cheng C; McLendon JM; et al.- Nucleic acids research, 2016
MicroRNAs (miRs) have emerged as key biological effectors in human health and disease. These small noncoding RNAs are incorporated into Argonaute (Ago) proteins, where they direct post-transcriptional gene silencing via base-pairing with target transcripts. Although miRs have become intriguing biological entities and attractive therapeutic targets, the translational impacts of miR research remain limited by a paucity of empirical miR targeting data, particularly in human primary tissues. Here, to improve our understanding of the diverse roles miRs play in cardiovascular function and disease, we applied high-throughput methods to globally profile miR:target interactions in human heart tissues. We deciphered Ago2:RNA interactions using crosslinking immunoprecipitation coupled with high-throughput sequencing (HITS-CLIP) to generate the first transcriptome-wide map of miR targeting events in human myocardium, detecting 4000 cardiac Ago2 binding sites across >2200 target transcripts. Our initial exploration of this interactome revealed an abundance of miR target sites in gene coding regions, including several sites pointing to new miR-29 functions in regulating cardiomyocyte calcium, growth and metabolism. Also, we uncovered several clinically-relevant interactions involving common genetic variants that alter miR targeting events in cardiomyopathy-associated genes. Overall, these data provide a critical resource for bolstering translational miR research in heart, and likely beyond.
LinkOut: [PMID: 27418678]
|
CLIP-seq Support 1 for dataset GSM4903833 | |
---|---|
Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / CTL_TD_21_a |
Location of target site | NM_030674 | 3UTR | UUAUUACAAAUACAAGAAUUUUGUACCUUAAAUAAGCCUCAUUUCUAUUUCUUCUUCAUUAAUUCUCCAUCUAGUC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 2 for dataset GSM4903834 | |
---|---|
Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | Dermal fibroblasts / CTL_TD_21_b |
Location of target site | NM_030674 | 3UTR | UCUUCAUUAAUUCUCCAUCUAGUCUUGUGAAAAAAAAAAAAAAAAAACCCUCAGAGAUAGUCUUUGUGAAGAGCUUCUGACAGAAUCACUGAGUACCUUCCUUCCCCCAGAUGAGGAAGACAAGGGG |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Accession Series | GSE161239 |
CLIP-seq Viewer | Link |
CLIP-seq Support 3 for dataset Chi_ControlB_2A8_130_50 | |
---|---|
Method / RBP | HITS-CLIP / AGO |
Cell line / Condition | HeLa / HeLa cell Control B |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 19536157 / Chi_HITSCLIP |
CLIP-seq Viewer | Link |
CLIP-seq Support 4 for dataset GSM1084041 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_arsenite_rep1 |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 5 for dataset GSM1084044 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_noarsenite_rep3 |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 6 for dataset GSM1084045 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_arsenite_rep3 |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 7 for dataset GSM1084046 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_noarsenite_rep4 |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 8 for dataset GSM1084047 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_arsenite_rep4 |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 9 for dataset GSM1084064 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_noemetine_AbnovaAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 10 for dataset GSM1084065 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_emetine_AbnovaAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 11 for dataset GSM1084066 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_noemetine_SantaCruzAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 12 for dataset GSM1084068 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_noemetine_SigmaAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 13 for dataset GSM1084069 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_emetine_SigmaAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 14 for dataset GSM1084074 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_nohippuristanol_rep1_SantaCruzAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 15 for dataset GSM1084075 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_hippuristanol_rep1_SantaCruzAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 16 for dataset GSM1084076 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_nohippuristanol_rep1_SigmaAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 17 for dataset GSM1084079 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_hippuristanol_rep2_AbnovaAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 18 for dataset GSM1084082 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_nohippuristanol_rep2_SigmaAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAA |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
CLIP-seq Support 19 for dataset GSM1084083 | |
---|---|
Method / RBP | HITS-CLIP / AGO2 |
Cell line / Condition | HEK293S / CLIP_hippuristanol_rep2_SigmaAb |
Location of target site | ENST00000398637.5 | 3UTR | UUGUGAAAAAAAAAAAAAAAAAAC |
Tools used in this analysis | TargetScan, miRTarCLIP, and Piranha |
Article / Accession Series | PMID: 23824327 / GSE44404 |
CLIP-seq Viewer | Link |
MiRNA-Target Expression Profile | |||||||
---|---|---|---|---|---|---|---|
|
MiRNA-Target Expression Profile (TCGA) | |||||||
---|---|---|---|---|---|---|---|
|
83 hsa-miR-4457 Target Genes:
Functional analysis:
ID![]() |
Target | Description | Validation methods |
![]() |
![]() |
|||||||
Strong evidence | Less strong evidence | |||||||||||
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
![]() |
|||||
MIRT098032 | SOBP | sine oculis binding protein homolog | ![]() |
![]() |
2 | 2 | ||||||
MIRT202580 | PCMTD2 | protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2 | ![]() |
![]() |
2 | 6 | ||||||
MIRT247137 | WEE1 | WEE1 G2 checkpoint kinase | ![]() |
![]() |
2 | 4 | ||||||
MIRT349266 | PTBP1 | polypyrimidine tract binding protein 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT363756 | EIF4EBP1 | eukaryotic translation initiation factor 4E binding protein 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT443586 | FAM84B | family with sequence similarity 84 member B | ![]() |
![]() |
2 | 2 | ||||||
MIRT452333 | EIF5AL1 | eukaryotic translation initiation factor 5A-like 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT453864 | ZBTB40 | zinc finger and BTB domain containing 40 | ![]() |
![]() |
2 | 4 | ||||||
MIRT471486 | PDE4D | phosphodiesterase 4D | ![]() |
![]() |
2 | 4 | ||||||
MIRT476290 | GMFB | glia maturation factor beta | ![]() |
![]() |
2 | 8 | ||||||
MIRT479897 | CCDC117 | coiled-coil domain containing 117 | ![]() |
![]() |
2 | 4 | ||||||
MIRT484251 | ANK1 | ankyrin 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT491473 | TMEM214 | transmembrane protein 214 | ![]() |
![]() |
2 | 2 | ||||||
MIRT493804 | GAN | gigaxonin | ![]() |
![]() |
2 | 6 | ||||||
MIRT499252 | VAV3 | vav guanine nucleotide exchange factor 3 | ![]() |
![]() |
2 | 4 | ||||||
MIRT502271 | HNRNPA1 | heterogeneous nuclear ribonucleoprotein A1 | ![]() |
![]() |
2 | 4 | ||||||
MIRT504651 | RPL9 | ribosomal protein L9 | ![]() |
![]() |
2 | 6 | ||||||
MIRT505211 | UBN2 | ubinuclein 2 | ![]() |
![]() |
2 | 8 | ||||||
MIRT508952 | SNRPB | small nuclear ribonucleoprotein polypeptides B and B1 | ![]() |
![]() |
2 | 4 | ||||||
MIRT512691 | POP1 | POP1 homolog, ribonuclease P/MRP subunit | ![]() |
![]() |
2 | 2 | ||||||
MIRT513320 | SCUBE3 | signal peptide, CUB domain and EGF like domain containing 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT513870 | HOXA5 | homeobox A5 | ![]() |
![]() |
2 | 2 | ||||||
MIRT517342 | ZNF529 | zinc finger protein 529 | ![]() |
![]() |
2 | 4 | ||||||
MIRT518947 | LSG1 | large 60S subunit nuclear export GTPase 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT520867 | SUGT1 | SGT1 homolog, MIS12 kinetochore complex assembly cochaperone | ![]() |
![]() |
2 | 2 | ||||||
MIRT528325 | GIGYF2 | GRB10 interacting GYF protein 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT531990 | SLCO1B3 | solute carrier organic anion transporter family member 1B3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT533298 | USP46 | ubiquitin specific peptidase 46 | ![]() |
![]() |
2 | 2 | ||||||
MIRT545257 | TRIM36 | tripartite motif containing 36 | ![]() |
![]() |
2 | 4 | ||||||
MIRT547038 | POGZ | pogo transposable element derived with ZNF domain | ![]() |
![]() |
2 | 2 | ||||||
MIRT556103 | MOAP1 | modulator of apoptosis 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT558321 | DR1 | down-regulator of transcription 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT558521 | CSRNP3 | cysteine and serine rich nuclear protein 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT560906 | TMED10 | transmembrane p24 trafficking protein 10 | ![]() |
![]() |
2 | 2 | ||||||
MIRT568448 | ARPP19 | cAMP regulated phosphoprotein 19 | ![]() |
![]() |
2 | 2 | ||||||
MIRT570586 | OTUD7B | OTU deubiquitinase 7B | ![]() |
![]() |
2 | 2 | ||||||
MIRT572799 | SIGLEC14 | sialic acid binding Ig like lectin 14 | ![]() |
![]() |
2 | 2 | ||||||
MIRT573863 | C9orf78 | chromosome 9 open reading frame 78 | ![]() |
![]() |
2 | 2 | ||||||
MIRT575058 | P2ry1 | purinergic receptor P2Y, G-protein coupled 1 | ![]() |
![]() |
2 | 5 | ||||||
MIRT609931 | SLC38A1 | solute carrier family 38 member 1 | ![]() |
![]() |
2 | 4 | ||||||
MIRT610836 | ZNF585A | zinc finger protein 585A | ![]() |
![]() |
2 | 4 | ||||||
MIRT611474 | P2RY1 | purinergic receptor P2Y1 | ![]() |
![]() |
2 | 7 | ||||||
MIRT613569 | YY2 | YY2 transcription factor | ![]() |
![]() |
2 | 2 | ||||||
MIRT618626 | GREB1 | growth regulation by estrogen in breast cancer 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT620606 | SAP30 | Sin3A associated protein 30 | ![]() |
![]() |
2 | 2 | ||||||
MIRT621017 | CLSTN3 | calsyntenin 3 | ![]() |
![]() |
2 | 4 | ||||||
MIRT635314 | FAM179A | TOG array regulator of axonemal microtubules 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT635919 | GLTSCR2 | NOP53 ribosome biogenesis factor | ![]() |
![]() |
2 | 2 | ||||||
MIRT640598 | TM9SF4 | transmembrane 9 superfamily member 4 | ![]() |
![]() |
2 | 2 | ||||||
MIRT641784 | YWHAB | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta | ![]() |
![]() |
2 | 4 | ||||||
MIRT644067 | IQCE | IQ motif containing E | ![]() |
![]() |
2 | 2 | ||||||
MIRT648288 | TRAPPC2L | trafficking protein particle complex 2 like | ![]() |
![]() |
2 | 2 | ||||||
MIRT658085 | FOXR2 | forkhead box R2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT659077 | DEPTOR | DEP domain containing MTOR interacting protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT665307 | ZBTB37 | zinc finger and BTB domain containing 37 | ![]() |
![]() |
2 | 2 | ||||||
MIRT665975 | SYTL4 | synaptotagmin like 4 | ![]() |
![]() |
2 | 2 | ||||||
MIRT666302 | SLC25A25 | solute carrier family 25 member 25 | ![]() |
![]() |
2 | 2 | ||||||
MIRT674906 | RASSF9 | Ras association domain family member 9 | ![]() |
![]() |
2 | 2 | ||||||
MIRT680086 | THAP1 | THAP domain containing 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT681488 | DIP2A | disco interacting protein 2 homolog A | ![]() |
![]() |
2 | 2 | ||||||
MIRT691244 | DFNB59 | pejvakin | ![]() |
![]() |
2 | 2 | ||||||
MIRT692362 | AGTRAP | angiotensin II receptor associated protein | ![]() |
![]() |
2 | 2 | ||||||
MIRT693035 | MB21D1 | Mab-21 domain containing 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT693837 | STAT5A | signal transducer and activator of transcription 5A | ![]() |
![]() |
2 | 2 | ||||||
MIRT694479 | LRTOMT | leucine rich transmembrane and O-methyltransferase domain containing | ![]() |
![]() |
2 | 2 | ||||||
MIRT696070 | ZNF264 | zinc finger protein 264 | ![]() |
![]() |
2 | 2 | ||||||
MIRT696578 | TTC21B | tetratricopeptide repeat domain 21B | ![]() |
![]() |
2 | 2 | ||||||
MIRT696760 | MTFMT | mitochondrial methionyl-tRNA formyltransferase | ![]() |
![]() |
2 | 2 | ||||||
MIRT697307 | ZNF652 | zinc finger protein 652 | ![]() |
![]() |
2 | 2 | ||||||
MIRT700152 | RNF115 | ring finger protein 115 | ![]() |
![]() |
2 | 2 | ||||||
MIRT701056 | PARP2 | poly(ADP-ribose) polymerase 2 | ![]() |
![]() |
2 | 2 | ||||||
MIRT701198 | OTUD3 | OTU deubiquitinase 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT701335 | NSD1 | nuclear receptor binding SET domain protein 1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT702656 | ITGA3 | integrin subunit alpha 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT703618 | FBXO45 | F-box protein 45 | ![]() |
![]() |
2 | 2 | ||||||
MIRT704673 | CHTOP | chromatin target of PRMT1 | ![]() |
![]() |
2 | 2 | ||||||
MIRT708894 | ZNF780A | zinc finger protein 780A | ![]() |
![]() |
2 | 2 | ||||||
MIRT711620 | DGKH | diacylglycerol kinase eta | ![]() |
![]() |
2 | 2 | ||||||
MIRT713745 | TMEM81 | transmembrane protein 81 | ![]() |
![]() |
2 | 2 | ||||||
MIRT719712 | CD101 | CD101 molecule | ![]() |
![]() |
2 | 2 | ||||||
MIRT720294 | DLGAP3 | DLG associated protein 3 | ![]() |
![]() |
2 | 2 | ||||||
MIRT722606 | CCDC152 | coiled-coil domain containing 152 | ![]() |
![]() |
2 | 2 | ||||||
MIRT724566 | ACSBG1 | acyl-CoA synthetase bubblegum family member 1 | ![]() |
![]() |
2 | 2 |
miRNA-Drug Associations | ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
miRNA-Drug Resistance Associations | ||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|